Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pera.
Identifieur interne : 000718 ( Ncbi/Merge ); précédent : 000717; suivant : 000719Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pera.
Auteurs : Rúbia P. Purcino [Brésil] ; Camilo Lázaro Medina ; Daniel Martins De Souza ; Flávia Vischi Winck ; Eduardo Caruso Machado ; José Camilo Novello ; Marcos Antonio Machado ; Paulo MazzaferaSource :
- Journal of experimental botany [ 0022-0957 ] ; 2007.
English descriptors
- KwdEn :
- Amino Acids (metabolism), Citrus sinensis (enzymology), Citrus sinensis (metabolism), Citrus sinensis (microbiology), Electrophoresis, Gel, Two-Dimensional, Nitrates (metabolism), Nitrogen (metabolism), Plant Diseases, Plant Leaves (metabolism), Plant Leaves (microbiology), Plant Proteins (metabolism), Polyamines (metabolism), Proteome, Xylella (physiology), Xylem (metabolism), Xylem (microbiology).
- MESH :
- chemical , metabolism : Amino Acids, Nitrates, Nitrogen, Plant Proteins, Polyamines.
- enzymology : Citrus sinensis.
- metabolism : Citrus sinensis, Plant Leaves, Xylem.
- microbiology : Citrus sinensis, Plant Leaves, Xylem.
- physiology : Xylella.
- Electrophoresis, Gel, Two-Dimensional, Plant Diseases, Proteome.
Abstract
Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.
DOI: 10.1093/jxb/erm138
PubMed: 17609534
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Purcino</name><affiliation wicri:level="2"><nlm:affiliation>Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Medina, Camilo Lazaro" sort="Medina, Camilo Lazaro" uniqKey="Medina C" first="Camilo Lázaro" last="Medina">Camilo Lázaro Medina</name></author><author><name sortKey="Martins De Souza, Daniel" sort="Martins De Souza, Daniel" uniqKey="Martins De Souza D" first="Daniel" last="Martins De Souza">Daniel Martins De Souza</name></author><author><name sortKey="Winck, Flavia Vischi" sort="Winck, Flavia Vischi" uniqKey="Winck F" first="Flávia Vischi" last="Winck">Flávia Vischi Winck</name></author><author><name sortKey="Machado, Eduardo Caruso" sort="Machado, Eduardo Caruso" uniqKey="Machado E" first="Eduardo Caruso" last="Machado">Eduardo Caruso Machado</name></author><author><name sortKey="Novello, Jose Camilo" sort="Novello, Jose Camilo" uniqKey="Novello J" first="José Camilo" last="Novello">José Camilo Novello</name></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name></author><author><name sortKey="Mazzafera, Paulo" sort="Mazzafera, Paulo" uniqKey="Mazzafera P" first="Paulo" last="Mazzafera">Paulo Mazzafera</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17609534</idno><idno type="pmid">17609534</idno><idno type="doi">10.1093/jxb/erm138</idno><idno type="wicri:Area/PubMed/Corpus">000B79</idno><idno type="wicri:Area/PubMed/Curation">000B79</idno><idno type="wicri:Area/PubMed/Checkpoint">000B79</idno><idno type="wicri:Area/Ncbi/Merge">000718</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. 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Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17609534</PMID><DateCreated><Year>2007</Year><Month>9</Month><Day>28</Day></DateCreated><DateCompleted><Year>2008</Year><Month>01</Month><Day>29</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0022-0957</ISSN><JournalIssue CitedMedium="Print"><Volume>58</Volume><Issue>11</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J. Exp. Bot.</ISOAbbreviation></Journal><ArticleTitle>Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. 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Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Purcino</LastName><ForeName>Rúbia P</ForeName><Initials>RP</Initials><AffiliationInfo><Affiliation>Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Medina</LastName><ForeName>Camilo Lázaro</ForeName><Initials>CL</Initials></Author><Author ValidYN="Y"><LastName>Martins de Souza</LastName><ForeName>Daniel</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Winck</LastName><ForeName>Flávia Vischi</ForeName><Initials>FV</Initials></Author><Author ValidYN="Y"><LastName>Machado</LastName><ForeName>Eduardo Caruso</ForeName><Initials>EC</Initials></Author><Author ValidYN="Y"><LastName>Novello</LastName><ForeName>José Camilo</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Machado</LastName><ForeName>Marcos Antonio</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Mazzafera</LastName><ForeName>Paulo</ForeName><Initials>P</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>Jul</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino 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PubStatus="pubmed"><Year>2007</Year><Month>7</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>1</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>7</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17609534</ArticleId><ArticleId IdType="pii">erm138</ArticleId><ArticleId IdType="doi">10.1093/jxb/erm138</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region></list><tree><noCountry><name sortKey="Machado, Eduardo Caruso" sort="Machado, Eduardo Caruso" uniqKey="Machado E" first="Eduardo Caruso" last="Machado">Eduardo Caruso Machado</name><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name><name sortKey="Martins De Souza, Daniel" sort="Martins De Souza, Daniel" uniqKey="Martins De Souza D" first="Daniel" last="Martins De Souza">Daniel Martins De Souza</name><name sortKey="Mazzafera, Paulo" sort="Mazzafera, Paulo" uniqKey="Mazzafera P" first="Paulo" last="Mazzafera">Paulo Mazzafera</name><name sortKey="Medina, Camilo Lazaro" sort="Medina, Camilo Lazaro" uniqKey="Medina C" first="Camilo Lázaro" last="Medina">Camilo Lázaro Medina</name><name sortKey="Novello, Jose Camilo" sort="Novello, Jose Camilo" uniqKey="Novello J" first="José Camilo" last="Novello">José Camilo Novello</name><name sortKey="Winck, Flavia Vischi" sort="Winck, Flavia Vischi" uniqKey="Winck F" first="Flávia Vischi" last="Winck">Flávia Vischi Winck</name></noCountry><country name="Brésil"><region name="État de São Paulo"><name sortKey="Purcino, Rubia P" sort="Purcino, Rubia P" uniqKey="Purcino R" first="Rúbia P" last="Purcino">Rúbia P. 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