Characterization of the low affinity transport system for NO3− uptake by Citrus roots
Identifieur interne : 002F65 ( Main/Exploration ); précédent : 002F64; suivant : 002F66Characterization of the low affinity transport system for NO3− uptake by Citrus roots
Auteurs : M. Cerezo [Espagne] ; V. Flors [Espagne] ; F. Legaz [Espagne] ; Pilar Garc A-Agust N [Espagne]Source :
- Plant Science [ 0168-9452 ] ; 2000.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Nutrition.
English descriptors
- KwdEn :
- 2,4-DNP, 2,4-dinitrophenol, Absorption, Adenosinetriphosphatase, Affinity, Characterization, Citrus, DCCD, N,N’-dicyclohexyl-carbodiimide, DES, diethylstilbestrol, DTT, dithiothreitol, EDTA, ethylennediaminetetraacetic acid, FAD, flavin adenine dinucleotide, H+-ATPase, HATS or LATS, high-affinity or low-affinity transport systems, respectively, Inhibition, Inhibitors, Ionic channel, Isotope labelling, LATS, Membrane protein, Mes, 2(N-morpholino)-ethanesulfonic acid, NADH, β-nicotinamide adenine dinucleotide, reduced form, NR(A), nitrate reductase (activity), Nitrate uptake, Nitrates, Nitrogen Isotopes, Nutrition, PVPP, polyvinylpyrrolidone K 15, Regulation(control), Root, Tris, Tris(hydroxymethyl)-aminomethane, pH, ΔΨ, cell membrane electrical potential difference.
Abstract
Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO3− starvation, plants were transferred to solutions enriched with K15NO3 (96% atoms 15N excess) to measure 15NO3− uptake rates as a function of external 15NO3− concentrations. Two different NO3− uptake systems were found. Between 1 and 50 mM 15NO3− in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO3−], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO3− uptake rate measured at 5 or 30 mM external [15NO3−]. The extent of the inhibition depended on the [NO3−] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn, Gln) to the uptake solution resulted in a decrease in 15NO3− uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO42− did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3% and 41.4% respectively at 5mM external [15NO3−]. However, these compounds had little effect when 15NO3− uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced 15NO3− uptake by 68.8%–35.6%, at both external [15NO3−]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO3− uptake.
Url:
DOI: 10.1016/S0168-9452(00)00363-0
Affiliations:
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Le document en format XML
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<term>Affinity</term>
<term>Characterization</term>
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<term>DCCD, N,N’-dicyclohexyl-carbodiimide</term>
<term>DES, diethylstilbestrol</term>
<term>DTT, dithiothreitol</term>
<term>EDTA, ethylennediaminetetraacetic acid</term>
<term>FAD, flavin adenine dinucleotide</term>
<term>H+-ATPase</term>
<term>HATS or LATS, high-affinity or low-affinity transport systems, respectively</term>
<term>Inhibition</term>
<term>Inhibitors</term>
<term>Ionic channel</term>
<term>Isotope labelling</term>
<term>LATS</term>
<term>Membrane protein</term>
<term>Mes, 2(N-morpholino)-ethanesulfonic acid</term>
<term>NADH, β-nicotinamide adenine dinucleotide, reduced form</term>
<term>NR(A), nitrate reductase (activity)</term>
<term>Nitrate uptake</term>
<term>Nitrates</term>
<term>Nitrogen Isotopes</term>
<term>Nutrition</term>
<term>PVPP, polyvinylpyrrolidone K 15</term>
<term>Regulation(control)</term>
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<term>Tris, Tris(hydroxymethyl)-aminomethane</term>
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<term>ΔΨ, cell membrane electrical potential difference</term>
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<term>Canal ionique</term>
<term>Caractérisation</term>
<term>Citrus sinensis Poncirus trifoliata</term>
<term>Inhibition</term>
<term>Marquage isotopique</term>
<term>Nitrate</term>
<term>Nutrition</term>
<term>Protéine membranaire</term>
<term>Racine</term>
<term>Régulation</term>
<term>pH</term>
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<front><div type="abstract" xml:lang="en">Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO3− starvation, plants were transferred to solutions enriched with K15NO3 (96% atoms 15N excess) to measure 15NO3− uptake rates as a function of external 15NO3− concentrations. Two different NO3− uptake systems were found. Between 1 and 50 mM 15NO3− in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO3−], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO3− uptake rate measured at 5 or 30 mM external [15NO3−]. The extent of the inhibition depended on the [NO3−] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn, Gln) to the uptake solution resulted in a decrease in 15NO3− uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO42− did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3% and 41.4% respectively at 5mM external [15NO3−]. However, these compounds had little effect when 15NO3− uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced 15NO3− uptake by 68.8%–35.6%, at both external [15NO3−]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO3− uptake.</div>
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<name sortKey="Flors, V" sort="Flors, V" uniqKey="Flors V" first="V" last="Flors">V. Flors</name>
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