Characterization of the low affinity transport system for NO3− uptake by Citrus roots
Identifieur interne : 000A93 ( Istex/Curation ); précédent : 000A92; suivant : 000A94Characterization of the low affinity transport system for NO3− uptake by Citrus roots
Auteurs : M. Cerezo [Espagne] ; V. Flors [Espagne] ; F. Legaz [Espagne] ; Pilar Garc A-Agust N [Espagne]Source :
- Plant Science [ 0168-9452 ] ; 2000.
English descriptors
- KwdEn :
- 2,4-DNP, 2,4-dinitrophenol, Citrus, DCCD, N,N’-dicyclohexyl-carbodiimide, DES, diethylstilbestrol, DTT, dithiothreitol, EDTA, ethylennediaminetetraacetic acid, FAD, flavin adenine dinucleotide, H+-ATPase, HATS or LATS, high-affinity or low-affinity transport systems, respectively, Inhibitors, LATS, Mes, 2(N-morpholino)-ethanesulfonic acid, NADH, β-nicotinamide adenine dinucleotide, reduced form, NR(A), nitrate reductase (activity), Nitrate uptake, PVPP, polyvinylpyrrolidone K 15, Tris, Tris(hydroxymethyl)-aminomethane, ΔΨ, cell membrane electrical potential difference.
Abstract
Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO3− starvation, plants were transferred to solutions enriched with K15NO3 (96% atoms 15N excess) to measure 15NO3− uptake rates as a function of external 15NO3− concentrations. Two different NO3− uptake systems were found. Between 1 and 50 mM 15NO3− in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO3−], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO3− uptake rate measured at 5 or 30 mM external [15NO3−]. The extent of the inhibition depended on the [NO3−] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn, Gln) to the uptake solution resulted in a decrease in 15NO3− uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO42− did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3% and 41.4% respectively at 5mM external [15NO3−]. However, these compounds had little effect when 15NO3− uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced 15NO3− uptake by 68.8%–35.6%, at both external [15NO3−]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO3− uptake.
Url:
DOI: 10.1016/S0168-9452(00)00363-0
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A93
Links to Exploration step
ISTEX:15CAA853982BCC7BB6C3EFEE833DA518B399E92ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of the low affinity transport system for NO3− uptake by Citrus roots</title><author><name sortKey="Cerezo, M" sort="Cerezo, M" uniqKey="Cerezo M" first="M" last="Cerezo">M. Cerezo</name><affiliation wicri:level="1"><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071</wicri:regionArea></affiliation></author><author><name sortKey="Flors, V" sort="Flors, V" uniqKey="Flors V" first="V" last="Flors">V. Flors</name><affiliation wicri:level="1"><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071</wicri:regionArea></affiliation></author><author><name sortKey="Legaz, F" sort="Legaz, F" uniqKey="Legaz F" first="F" last="Legaz">F. Legaz</name><affiliation wicri:level="1"><mods:affiliation>Departamento de Citricultura, IVIA, Apartado Oficial 46113, Moncada, Valencia, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Citricultura, IVIA, Apartado Oficial 46113, Moncada, Valencia</wicri:regionArea></affiliation></author><author><name sortKey="Garc A Agust N, Pilar" sort="Garc A Agust N, Pilar" uniqKey="Garc A Agust N P" first="Pilar" last="Garc A-Agust N">Pilar Garc A-Agust N</name><affiliation wicri:level="1"><mods:affiliation>E-mail: garciap@exp.uji.es</mods:affiliation><country wicri:rule="url">Espagne</country></affiliation><affiliation wicri:level="1"><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:15CAA853982BCC7BB6C3EFEE833DA518B399E92A</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0168-9452(00)00363-0</idno><idno type="url">https://api.istex.fr/document/15CAA853982BCC7BB6C3EFEE833DA518B399E92A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A93</idno><idno type="wicri:Area/Istex/Curation">000A93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterization of the low affinity transport system for NO3− uptake by Citrus roots</title><author><name sortKey="Cerezo, M" sort="Cerezo, M" uniqKey="Cerezo M" first="M" last="Cerezo">M. Cerezo</name><affiliation wicri:level="1"><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071</wicri:regionArea></affiliation></author><author><name sortKey="Flors, V" sort="Flors, V" uniqKey="Flors V" first="V" last="Flors">V. Flors</name><affiliation wicri:level="1"><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071</wicri:regionArea></affiliation></author><author><name sortKey="Legaz, F" sort="Legaz, F" uniqKey="Legaz F" first="F" last="Legaz">F. Legaz</name><affiliation wicri:level="1"><mods:affiliation>Departamento de Citricultura, IVIA, Apartado Oficial 46113, Moncada, Valencia, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Citricultura, IVIA, Apartado Oficial 46113, Moncada, Valencia</wicri:regionArea></affiliation></author><author><name sortKey="Garc A Agust N, Pilar" sort="Garc A Agust N, Pilar" uniqKey="Garc A Agust N P" first="Pilar" last="Garc A-Agust N">Pilar Garc A-Agust N</name><affiliation wicri:level="1"><mods:affiliation>E-mail: garciap@exp.uji.es</mods:affiliation><country wicri:rule="url">Espagne</country></affiliation><affiliation wicri:level="1"><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I, Castellon12071</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Science</title><title level="j" type="abbrev">PSL</title><idno type="ISSN">0168-9452</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">160</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="95">95</biblScope><biblScope unit="page" to="104">104</biblScope></imprint><idno type="ISSN">0168-9452</idno></series><idno type="istex">15CAA853982BCC7BB6C3EFEE833DA518B399E92A</idno><idno type="DOI">10.1016/S0168-9452(00)00363-0</idno><idno type="PII">S0168-9452(00)00363-0</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-9452</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2,4-DNP, 2,4-dinitrophenol</term><term>Citrus</term><term>DCCD, N,N’-dicyclohexyl-carbodiimide</term><term>DES, diethylstilbestrol</term><term>DTT, dithiothreitol</term><term>EDTA, ethylennediaminetetraacetic acid</term><term>FAD, flavin adenine dinucleotide</term><term>H+-ATPase</term><term>HATS or LATS, high-affinity or low-affinity transport systems, respectively</term><term>Inhibitors</term><term>LATS</term><term>Mes, 2(N-morpholino)-ethanesulfonic acid</term><term>NADH, β-nicotinamide adenine dinucleotide, reduced form</term><term>NR(A), nitrate reductase (activity)</term><term>Nitrate uptake</term><term>PVPP, polyvinylpyrrolidone K 15</term><term>Tris, Tris(hydroxymethyl)-aminomethane</term><term>ΔΨ, cell membrane electrical potential difference</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO3− starvation, plants were transferred to solutions enriched with K15NO3 (96% atoms 15N excess) to measure 15NO3− uptake rates as a function of external 15NO3− concentrations. Two different NO3− uptake systems were found. Between 1 and 50 mM 15NO3− in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO3−], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO3− uptake rate measured at 5 or 30 mM external [15NO3−]. The extent of the inhibition depended on the [NO3−] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn, Gln) to the uptake solution resulted in a decrease in 15NO3− uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO42− did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3% and 41.4% respectively at 5mM external [15NO3−]. However, these compounds had little effect when 15NO3− uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced 15NO3− uptake by 68.8%–35.6%, at both external [15NO3−]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO3− uptake.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A93 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000A93 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:15CAA853982BCC7BB6C3EFEE833DA518B399E92A
|texte= Characterization of the low affinity transport system for NO3− uptake by Citrus roots
}}
| This area was generated with Dilib version V0.6.25. |  |