OrangerV1, PascalFrancis, Corpus, bibRecord, 000929

Characterization of the low affinity transport system for NO3- uptake by Citrus roots

Identifieur interne : 000929 ( PascalFrancis/Corpus ); précédent : 000928; suivant : 000930

Characterization of the low affinity transport system for NO3- uptake by Citrus roots

Auteurs : M. Cerezo ; V. Flor ; F. Legaz ; Pilar Garcia-Agustin

Source :

Plant science : (Limerick) [ 0168-9452 ] ; 2000.

RBID : Pascal:01-0106859

Descripteurs français

Pascal (Inist)
- Caractérisation, Nutrition, Absorption, Protéine membranaire, Canal ionique, Adenosinetriphosphatase, Affinité, Racine, Marquage isotopique, Nitrate, Azote Isotope, Inhibition, pH, Régulation, Citrus sinensis Poncirus trifoliata.

English descriptors

KwdEn :
- Absorption, Adenosinetriphosphatase, Affinity, Characterization, Inhibition, Ionic channel, Isotope labelling, Membrane protein, Nitrates, Nitrogen Isotopes, Nutrition, Regulation(control), Root, pH.

Abstract

Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO₃^- starvation, plants were transferred to solutions enriched with K¹⁵NO₃ (96% atoms ¹⁵N excess) to measure ¹³NO₃ uptake rates as a function of external ¹⁵NO₃ concentrations. Two different NO₃ uptake systems were found. Between 1 and 50 mM ¹⁵NO₃ in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO₃], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO₃ uptake rate measured at 5 or 30 mM external [¹⁵NO₃]. The extent of the inhibition depended on the [NO₃] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn. Gln) to the uptake solution resulted in a decrease in ¹⁵NO₃ uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO²₄ did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3%, and 41.4% respectively at 5mM external [¹⁵NO₃]. However, these compounds had little effect when ¹⁵NO₃ uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced ¹⁵NO₃ uptake by 68.8%-35.6%, at both external [¹⁵NO₃]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO₃ uptake.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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N21				`@1 071`

Format Inist (serveur)

NO :	PASCAL 01-0106859 INIST
ET :	Characterization of the low affinity transport system for NO₃^- uptake by Citrus roots
AU :	CEREZO (M.); FLOR (V.); LEGAZ (F.); GARCIA-AGUSTIN (Pilar)
AF :	Departamento de Ciencias Experimentales, Unidad de Biotecnología Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I/Castellon 12071/Espagne (1 aut., 2 aut., 4 aut.); Departamento de Citricultura, IVIA, Apartado Oficial 46113/Moncada, Valencia/Espagne (3 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant science : (Limerick); ISSN 0168-9452; Coden PLSCE4; Irlande; Da. 2000; Vol. 160; No. 1; Pp. 95-104; Bibl. 51 ref.
LA :	Anglais
EA :	Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO₃^- starvation, plants were transferred to solutions enriched with K¹⁵NO₃ (96% atoms ¹⁵N excess) to measure ¹³NO₃ uptake rates as a function of external ¹⁵NO₃ concentrations. Two different NO₃ uptake systems were found. Between 1 and 50 mM ¹⁵NO₃ in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO₃], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO₃ uptake rate measured at 5 or 30 mM external [¹⁵NO₃]. The extent of the inhibition depended on the [NO₃] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn. Gln) to the uptake solution resulted in a decrease in ¹⁵NO₃ uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO²₄ did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3%, and 41.4% respectively at 5mM external [¹⁵NO₃]. However, these compounds had little effect when ¹⁵NO₃ uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced ¹⁵NO₃ uptake by 68.8%-35.6%, at both external [¹⁵NO₃]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO₃ uptake.
CC :	002A10C; 002A32E05C
FD :	Caractérisation; Nutrition; Absorption; Protéine membranaire; Canal ionique; Adenosinetriphosphatase; Affinité; Racine; Marquage isotopique; Nitrate; Azote Isotope; Inhibition; pH; Régulation; Citrus sinensis Poncirus trifoliata
FG :	Hydrolases; Enzyme; Agrume; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Nutriment; Elément minéral
ED :	Characterization; Nutrition; Absorption; Membrane protein; Ionic channel; Adenosinetriphosphatase; Affinity; Root; Isotope labelling; Nitrates; Nitrogen Isotopes; Inhibition; pH; Regulation(control)
EG :	Hydrolases; Enzyme; Citrus fruit; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Nutrient; Inorganic element
SD :	Caracterización; Nutrición; Absorción; Proteína membranar; Canal iónico; Adenosinetriphosphatase; Afinidad; Raíz; Marcación isotópica; Nitrato; Nitrógeno Isótopo; Inhibición; pH; Regulación
LO :	INIST-15982.354000094081890100
ID :	01-0106859

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Pascal:01-0106859

Le document en format XML

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<sup>-</sup>
 uptake by Citrus roots</title>
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<term>Ionic channel</term>
<term>Isotope labelling</term>
<term>Membrane protein</term>
<term>Nitrates</term>
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<term>Nitrate</term>
<term>Azote Isotope</term>
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<front><div type="abstract" xml:lang="en">Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO<sub>3</sub>
<sup>-</sup>
 starvation, plants were transferred to solutions enriched with K<sup>15</sup>
NO<sub>3</sub>
 (96% atoms <sup>15</sup>
N excess) to measure <sup>13</sup>
NO<sub>3</sub>
 uptake rates as a function of external <sup>15</sup>
NO<sub>3</sub>
 concentrations. Two different NO<sub>3</sub>
 uptake systems were found. Between 1 and 50 mM <sup>15</sup>
NO<sub>3</sub>
 in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO<sub>3</sub>
], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO<sub>3</sub>
 uptake rate measured at 5 or 30 mM external [<sup>15</sup>
NO<sub>3</sub>
]. The extent of the inhibition depended on the [NO<sub>3</sub>
] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn. Gln) to the uptake solution resulted in a decrease in <sup>15</sup>
NO<sub>3</sub>
 uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO<sup>2</sup>
<sub>4</sub>
 did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3%, and 41.4% respectively at 5mM external [<sup>15</sup>
NO<sub>3</sub>
]. However, these compounds had little effect when <sup>15</sup>
NO<sub>3</sub>
 uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced <sup>15</sup>
NO<sub>3</sub>
 uptake by 68.8%-35.6%, at both external [<sup>15</sup>
NO<sub>3</sub>
]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO<sub>3</sub>
 uptake.</div>
</front>
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<fA06><s2>1</s2>
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<fA08 i1="01" i2="1" l="ENG"><s1>Characterization of the low affinity transport system for NO<sub>3</sub>
<sup>-</sup>
 uptake by Citrus roots</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>CEREZO (M.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>FLOR (V.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>LEGAZ (F.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>GARCIA-AGUSTIN (Pilar)</s1>
</fA11>
<fA14 i1="01"><s1>Departamento de Ciencias Experimentales, Unidad de Biotecnología Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I</s1>
<s2>Castellon 12071</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Departamento de Citricultura, IVIA, Apartado Oficial 46113</s1>
<s2>Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA20><s1>95-104</s1>
</fA20>
<fA21><s1>2000</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>15982</s2>
<s5>354000094081890100</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2001 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>51 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>01-0106859</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Plant science : (Limerick)</s0>
</fA64>
<fA66 i1="01"><s0>IRL</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO<sub>3</sub>
<sup>-</sup>
 starvation, plants were transferred to solutions enriched with K<sup>15</sup>
NO<sub>3</sub>
 (96% atoms <sup>15</sup>
N excess) to measure <sup>13</sup>
NO<sub>3</sub>
 uptake rates as a function of external <sup>15</sup>
NO<sub>3</sub>
 concentrations. Two different NO<sub>3</sub>
 uptake systems were found. Between 1 and 50 mM <sup>15</sup>
NO<sub>3</sub>
 in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO<sub>3</sub>
], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO<sub>3</sub>
 uptake rate measured at 5 or 30 mM external [<sup>15</sup>
NO<sub>3</sub>
]. The extent of the inhibition depended on the [NO<sub>3</sub>
] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn. Gln) to the uptake solution resulted in a decrease in <sup>15</sup>
NO<sub>3</sub>
 uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO<sup>2</sup>
<sub>4</sub>
 did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3%, and 41.4% respectively at 5mM external [<sup>15</sup>
NO<sub>3</sub>
]. However, these compounds had little effect when <sup>15</sup>
NO<sub>3</sub>
 uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced <sup>15</sup>
NO<sub>3</sub>
 uptake by 68.8%-35.6%, at both external [<sup>15</sup>
NO<sub>3</sub>
]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO<sub>3</sub>
 uptake.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A10C</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A32E05C</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Caractérisation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Characterization</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Caracterización</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Nutrition</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Nutrition</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Nutrición</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Absorption</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Absorption</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Absorción</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Protéine membranaire</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Membrane protein</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Proteína membranar</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Canal ionique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Ionic channel</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Canal iónico</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Adenosinetriphosphatase</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Adenosinetriphosphatase</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Adenosinetriphosphatase</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Affinité</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Affinity</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Afinidad</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Racine</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Root</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Raíz</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Marquage isotopique</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Isotope labelling</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Marcación isotópica</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Nitrate</s0>
<s2>NA</s2>
<s2>FX</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Nitrates</s0>
<s2>NA</s2>
<s2>FX</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Nitrato</s0>
<s2>NA</s2>
<s2>FX</s2>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Azote Isotope</s0>
<s2>NC</s2>
<s2>NA</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Nitrogen Isotopes</s0>
<s2>NC</s2>
<s2>NA</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Nitrógeno Isótopo</s0>
<s2>NC</s2>
<s2>NA</s2>
<s5>16</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Inhibition</s0>
<s5>33</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Inhibition</s0>
<s5>33</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Inhibición</s0>
<s5>33</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>pH</s0>
<s5>34</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>pH</s0>
<s5>34</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>pH</s0>
<s5>34</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Régulation</s0>
<s5>35</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Regulation(control)</s0>
<s5>35</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Regulación</s0>
<s5>35</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Citrus sinensis  Poncirus trifoliata</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>77</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Enzyme</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Enzyme</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Enzima</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Agrume</s0>
<s5>40</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>40</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Agrios</s0>
<s5>40</s5>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
<s5>46</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
<s5>46</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
<s5>46</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Nutriment</s0>
<s5>50</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Nutrient</s0>
<s5>50</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Nutriente</s0>
<s5>50</s5>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Elément minéral</s0>
<s5>51</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Inorganic element</s0>
<s5>51</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Elemento inorgánico</s0>
<s5>51</s5>
</fC07>
<fN21><s1>071</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 01-0106859 INIST</NO>
<ET>Characterization of the low affinity transport system for NO<sub>3</sub>
<sup>-</sup>
 uptake by Citrus roots</ET>
<AU>CEREZO (M.); FLOR (V.); LEGAZ (F.); GARCIA-AGUSTIN (Pilar)</AU>
<AF>Departamento de Ciencias Experimentales, Unidad de Biotecnología Vegetal, ESTCE, Campus Riu Sec, Universidad Jaume I/Castellon 12071/Espagne (1 aut., 2 aut., 4 aut.); Departamento de Citricultura, IVIA, Apartado Oficial 46113/Moncada, Valencia/Espagne (3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant science : (Limerick); ISSN 0168-9452; Coden PLSCE4; Irlande; Da. 2000; Vol. 160; No. 1; Pp. 95-104; Bibl. 51 ref.</SO>
<LA>Anglais</LA>
<EA>Three-month old citrange Troyer (hybrid of Citrus sinensis x Poncirus trifoliata) seedlings were grown hydroponically and, after a period of NO<sub>3</sub>
<sup>-</sup>
 starvation, plants were transferred to solutions enriched with K<sup>15</sup>
NO<sub>3</sub>
 (96% atoms <sup>15</sup>
N excess) to measure <sup>13</sup>
NO<sub>3</sub>
 uptake rates as a function of external <sup>15</sup>
NO<sub>3</sub>
 concentrations. Two different NO<sub>3</sub>
 uptake systems were found. Between 1 and 50 mM <sup>15</sup>
NO<sub>3</sub>
 in the uptake solution medium, the uptake rate increased linearly due to the low affinity transport system (LATS). Nitrate reductase activity showed the same response to external [NO<sub>3</sub>
], and also appears to be regulated by the rate of nitrate uptake. Nitrate pre-treatments had a represive effect on NO<sub>3</sub>
 uptake rate measured at 5 or 30 mM external [<sup>15</sup>
NO<sub>3</sub>
]. The extent of the inhibition depended on the [NO<sub>3</sub>
] during the pre-treatment and in the uptake solution. These results suggest that the LATS of Citrus seedlings is under feedback control by the N status of the plant. Accordingly, addition of amino acids (Glu, Asp, Asn. Gln) to the uptake solution resulted in a decrease in <sup>15</sup>
NO<sub>3</sub>
 uptake rate. However, the inactivation of nitrate reductase activity after treatment of the seedlings with either 100 or 500 μM WO<sup>2</sup>
<sub>4</sub>
 did not affect the activity of the LATS. Metabolic uncouplers, 2,4-DNP and KCN, reduced the uptake rate by 43.3%, and 41.4% respectively at 5mM external [<sup>15</sup>
NO<sub>3</sub>
]. However, these compounds had little effect when <sup>15</sup>
NO<sub>3</sub>
 uptake was assayed at 30 mM external concentration. The ATPase inhibitors DCCD and DES reduced <sup>15</sup>
NO<sub>3</sub>
 uptake by 68.8%-35.6%, at both external [<sup>15</sup>
NO<sub>3</sub>
]. Nitrate uptake by the LATS declined with the increase of the solution pH beyond pH 4. The data presented are discussed in the context of the kinetics, energy dependence and regulation of NO<sub>3</sub>
 uptake.</EA>
<CC>002A10C; 002A32E05C</CC>
<FD>Caractérisation; Nutrition; Absorption; Protéine membranaire; Canal ionique; Adenosinetriphosphatase; Affinité; Racine; Marquage isotopique; Nitrate; Azote Isotope; Inhibition; pH; Régulation; Citrus sinensis  Poncirus trifoliata</FD>
<FG>Hydrolases; Enzyme; Agrume; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Nutriment; Elément minéral</FG>
<ED>Characterization; Nutrition; Absorption; Membrane protein; Ionic channel; Adenosinetriphosphatase; Affinity; Root; Isotope labelling; Nitrates; Nitrogen Isotopes; Inhibition; pH; Regulation(control)</ED>
<EG>Hydrolases; Enzyme; Citrus fruit; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Nutrient; Inorganic element</EG>
<SD>Caracterización; Nutrición; Absorción; Proteína membranar; Canal iónico; Adenosinetriphosphatase; Afinidad; Raíz; Marcación isotópica; Nitrato; Nitrógeno Isótopo; Inhibición; pH; Regulación</SD>
<LO>INIST-15982.354000094081890100</LO>
<ID>01-0106859</ID>
</server>
</inist>
</record>

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Characterization of the low affinity transport system for NO3- uptake by Citrus roots

Characterization of the low affinity transport system for NO3- uptake by Citrus roots

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