Mixotrophy of Platanthera minor, an orchid associated with ectomycorrhiza-forming Ceratobasidiaceae fungi.
Identifieur interne : 002179 ( Main/Corpus ); précédent : 002178; suivant : 002180Mixotrophy of Platanthera minor, an orchid associated with ectomycorrhiza-forming Ceratobasidiaceae fungi.
Auteurs : Takahiro Yagame ; Takamichi Orihara ; Marc-André Selosse ; Masahide Yamato ; Koji IwaseSource :
- The New phytologist [ 1469-8137 ] ; 2012.
English descriptors
- KwdEn :
- Autotrophic Processes (physiology), Base Sequence (MeSH), Basidiomycota (cytology), Basidiomycota (genetics), Basidiomycota (physiology), Bayes Theorem (MeSH), Carbon Isotopes (MeSH), DNA, Intergenic (genetics), Ecosystem (MeSH), Fruiting Bodies, Fungal (cytology), Fruiting Bodies, Fungal (physiology), Japan (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (cytology), Mycorrhizae (genetics), Mycorrhizae (physiology), Nitrogen Isotopes (MeSH), Orchidaceae (cytology), Orchidaceae (microbiology), Orchidaceae (physiology), Phylogeny (MeSH).
- MESH :
- chemical , genetics : DNA, Intergenic.
- chemical : Carbon Isotopes, Nitrogen Isotopes.
- geographic : Japan.
- cytology : Basidiomycota, Fruiting Bodies, Fungal, Mycorrhizae, Orchidaceae.
- genetics : Basidiomycota, Mycorrhizae.
- microbiology : Orchidaceae.
- physiology : Autotrophic Processes, Basidiomycota, Fruiting Bodies, Fungal, Mycorrhizae, Orchidaceae.
- Base Sequence, Bayes Theorem, Ecosystem, Molecular Sequence Data, Phylogeny.
Abstract
• We investigated the fungal symbionts and carbon nutrition of a Japanese forest photosynthetic orchid, Platanthera minor, whose ecology suggests a mixotrophic syndrome, that is, a mycorrhizal association with ectomycorrhiza (ECM)-forming fungi and partial exploitation of fungal carbon. • We performed molecular identification of symbionts by PCR amplifications of the fungal ribosomal DNA on hyphal coils extracted from P. minor roots. We tested for a (13)C and (15)N enrichment characteristic of mixotrophic plants. We also tested the ectomycorrhizal abilities of orchid symbionts using a new protocol of direct inoculation of hyphal coils onto roots of Pinus densiflora seedlings. • In phylogenetic analyses, most isolated fungi were close to ECM-forming Ceratobasidiaceae clades previously detected from a few fully heterotrophic orchids or environmental ectomycorrhiza surveys. The direct inoculation of fungal coils of these fungi resulted in ectomycorrhiza formation on P. densiflora seedlings. Stable isotope analyses indicated mixotrophic nutrition of P. minor, with fungal carbon contributing from 50% to 65%. • This is the first evidence of photosynthetic orchids associated with ectomycorrhizal Ceratobasidiaceae taxa, confirming the evolution of mixotrophy in the Orchideae orchid tribe, and of ectomycorrhizal abilities in the Ceratobasidiaceae. Our new ectomycorrhiza formation technique may enhance the study of unculturable orchid mycorrhizal fungi.
DOI: 10.1111/j.1469-8137.2011.03896.x
PubMed: 21995447
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pubmed:21995447Le document en format XML
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Yamato</name></author><author><name sortKey="Iwase, Koji" sort="Iwase, Koji" uniqKey="Iwase K" first="Koji" last="Iwase">Koji Iwase</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:21995447</idno><idno type="pmid">21995447</idno><idno type="doi">10.1111/j.1469-8137.2011.03896.x</idno><idno type="wicri:Area/Main/Corpus">002179</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002179</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mixotrophy of Platanthera minor, an orchid associated with ectomycorrhiza-forming Ceratobasidiaceae fungi.</title><author><name sortKey="Yagame, Takahiro" sort="Yagame, Takahiro" uniqKey="Yagame T" first="Takahiro" last="Yagame">Takahiro Yagame</name><affiliation><nlm:affiliation>Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University 4-101 Koyama-Minami, Tottori, 680-8553, Japan. t_yagame@muses.tottori-u.ac.jp</nlm:affiliation></affiliation></author><author><name sortKey="Orihara, Takamichi" sort="Orihara, Takamichi" uniqKey="Orihara T" first="Takamichi" last="Orihara">Takamichi Orihara</name></author><author><name sortKey="Selosse, Marc Andre" sort="Selosse, Marc Andre" uniqKey="Selosse M" first="Marc-André" last="Selosse">Marc-André Selosse</name></author><author><name sortKey="Yamato, Masahide" sort="Yamato, Masahide" uniqKey="Yamato M" first="Masahide" last="Yamato">Masahide Yamato</name></author><author><name sortKey="Iwase, Koji" sort="Iwase, Koji" uniqKey="Iwase K" first="Koji" last="Iwase">Koji Iwase</name></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Autotrophic Processes (physiology)</term><term>Base Sequence (MeSH)</term><term>Basidiomycota (cytology)</term><term>Basidiomycota (genetics)</term><term>Basidiomycota (physiology)</term><term>Bayes Theorem (MeSH)</term><term>Carbon Isotopes (MeSH)</term><term>DNA, Intergenic (genetics)</term><term>Ecosystem (MeSH)</term><term>Fruiting Bodies, Fungal (cytology)</term><term>Fruiting Bodies, Fungal (physiology)</term><term>Japan (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (cytology)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (physiology)</term><term>Nitrogen Isotopes (MeSH)</term><term>Orchidaceae (cytology)</term><term>Orchidaceae (microbiology)</term><term>Orchidaceae (physiology)</term><term>Phylogeny (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Intergenic</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon Isotopes</term><term>Nitrogen Isotopes</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Japan</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Basidiomycota</term><term>Fruiting Bodies, Fungal</term><term>Mycorrhizae</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Autotrophic Processes</term><term>Basidiomycota</term><term>Fruiting Bodies, Fungal</term><term>Mycorrhizae</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Bayes Theorem</term><term>Ecosystem</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">• We investigated the fungal symbionts and carbon nutrition of a Japanese forest photosynthetic orchid, Platanthera minor, whose ecology suggests a mixotrophic syndrome, that is, a mycorrhizal association with ectomycorrhiza (ECM)-forming fungi and partial exploitation of fungal carbon. • We performed molecular identification of symbionts by PCR amplifications of the fungal ribosomal DNA on hyphal coils extracted from P. minor roots. We tested for a (13)C and (15)N enrichment characteristic of mixotrophic plants. We also tested the ectomycorrhizal abilities of orchid symbionts using a new protocol of direct inoculation of hyphal coils onto roots of Pinus densiflora seedlings. • In phylogenetic analyses, most isolated fungi were close to ECM-forming Ceratobasidiaceae clades previously detected from a few fully heterotrophic orchids or environmental ectomycorrhiza surveys. The direct inoculation of fungal coils of these fungi resulted in ectomycorrhiza formation on P. densiflora seedlings. Stable isotope analyses indicated mixotrophic nutrition of P. minor, with fungal carbon contributing from 50% to 65%. • This is the first evidence of photosynthetic orchids associated with ectomycorrhizal Ceratobasidiaceae taxa, confirming the evolution of mixotrophy in the Orchideae orchid tribe, and of ectomycorrhizal abilities in the Ceratobasidiaceae. Our new ectomycorrhiza formation technique may enhance the study of unculturable orchid mycorrhizal fungi.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21995447</PMID><DateCompleted><Year>2012</Year><Month>04</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>193</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Jan</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Mixotrophy of Platanthera minor, an orchid associated with ectomycorrhiza-forming Ceratobasidiaceae fungi.</ArticleTitle><Pagination><MedlinePgn>178-87</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2011.03896.x</ELocationID><Abstract><AbstractText>• We investigated the fungal symbionts and carbon nutrition of a Japanese forest photosynthetic orchid, Platanthera minor, whose ecology suggests a mixotrophic syndrome, that is, a mycorrhizal association with ectomycorrhiza (ECM)-forming fungi and partial exploitation of fungal carbon. • We performed molecular identification of symbionts by PCR amplifications of the fungal ribosomal DNA on hyphal coils extracted from P. minor roots. We tested for a (13)C and (15)N enrichment characteristic of mixotrophic plants. We also tested the ectomycorrhizal abilities of orchid symbionts using a new protocol of direct inoculation of hyphal coils onto roots of Pinus densiflora seedlings. • In phylogenetic analyses, most isolated fungi were close to ECM-forming Ceratobasidiaceae clades previously detected from a few fully heterotrophic orchids or environmental ectomycorrhiza surveys. The direct inoculation of fungal coils of these fungi resulted in ectomycorrhiza formation on P. densiflora seedlings. Stable isotope analyses indicated mixotrophic nutrition of P. minor, with fungal carbon contributing from 50% to 65%. • This is the first evidence of photosynthetic orchids associated with ectomycorrhizal Ceratobasidiaceae taxa, confirming the evolution of mixotrophy in the Orchideae orchid tribe, and of ectomycorrhizal abilities in the Ceratobasidiaceae. Our new ectomycorrhiza formation technique may enhance the study of unculturable orchid mycorrhizal fungi.</AbstractText><CopyrightInformation>© 2011 The Authors. 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