Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis
Identifieur interne : 000141 ( Pmc/Curation ); précédent : 000140; suivant : 000142Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis
Auteurs : Ivett Baksa [Hongrie] ; Tibor Nagy [Hongrie] ; Endre Barta [Hongrie] ; Zoltán Havelda [Hongrie] ; Éva Várallyay [Hongrie] ; Dániel Silhavy [Hongrie] ; J Zsef Burgyán [Hongrie] ; György Szittya [Hongrie]Source :
- BMC Genomics [ 1471-2164 ] ; 2015.
Abstract
We used a comprehensive molecular approach to detect and to experimentally validate
The identified conserved and novel miRNAs along with their target mRNAs also provides a tissue specific atlas of known and new miRNA expression and their cleaved target mRNAs of
The online version of this article (doi:10.1186/s12864-015-2209-6) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12864-015-2209-6
PubMed: 26626050
PubMed Central: 4667520
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000141
Links to Exploration step
PMC:4667520Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of <italic>Nicotiana benthamiana</italic> microRNAs and their targets using high throughput sequencing and degradome analysis</title><author><name sortKey="Baksa, Ivett" sort="Baksa, Ivett" uniqKey="Baksa I" first="Ivett" last="Baksa">Ivett Baksa</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Nagy, Tibor" sort="Nagy, Tibor" uniqKey="Nagy T" first="Tibor" last="Nagy">Tibor Nagy</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Barta, Endre" sort="Barta, Endre" uniqKey="Barta E" first="Endre" last="Barta">Endre Barta</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Havelda, Zoltan" sort="Havelda, Zoltan" uniqKey="Havelda Z" first="Zoltán" last="Havelda">Zoltán Havelda</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Varallyay, Eva" sort="Varallyay, Eva" uniqKey="Varallyay E" first="Éva" last="Várallyay">Éva Várallyay</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Silhavy, Daniel" sort="Silhavy, Daniel" uniqKey="Silhavy D" first="Dániel" last="Silhavy">Dániel Silhavy</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Burgyan, J Zsef" sort="Burgyan, J Zsef" uniqKey="Burgyan J" first="J Zsef" last="Burgyán">J Zsef Burgyán</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Szittya, Gyorgy" sort="Szittya, Gyorgy" uniqKey="Szittya G" first="György" last="Szittya">György Szittya</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26626050</idno><idno type="pmc">4667520</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667520</idno><idno type="RBID">PMC:4667520</idno><idno type="doi">10.1186/s12864-015-2209-6</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000141</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000141</idno><idno type="wicri:Area/Pmc/Curation">000141</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000141</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of <italic>Nicotiana benthamiana</italic> microRNAs and their targets using high throughput sequencing and degradome analysis</title><author><name sortKey="Baksa, Ivett" sort="Baksa, Ivett" uniqKey="Baksa I" first="Ivett" last="Baksa">Ivett Baksa</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Nagy, Tibor" sort="Nagy, Tibor" uniqKey="Nagy T" first="Tibor" last="Nagy">Tibor Nagy</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Barta, Endre" sort="Barta, Endre" uniqKey="Barta E" first="Endre" last="Barta">Endre Barta</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Havelda, Zoltan" sort="Havelda, Zoltan" uniqKey="Havelda Z" first="Zoltán" last="Havelda">Zoltán Havelda</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Varallyay, Eva" sort="Varallyay, Eva" uniqKey="Varallyay E" first="Éva" last="Várallyay">Éva Várallyay</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Silhavy, Daniel" sort="Silhavy, Daniel" uniqKey="Silhavy D" first="Dániel" last="Silhavy">Dániel Silhavy</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Burgyan, J Zsef" sort="Burgyan, J Zsef" uniqKey="Burgyan J" first="J Zsef" last="Burgyán">J Zsef Burgyán</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author><author><name sortKey="Szittya, Gyorgy" sort="Szittya, Gyorgy" uniqKey="Szittya G" first="György" last="Szittya">György Szittya</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</nlm:aff><country xml:lang="fr">Hongrie</country><wicri:regionArea>Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p><italic>Nicotiana benthamiana</italic> is a widely used model plant species for research on plant-pathogen interactions as well as other areas of plant science. It can be easily transformed or agroinfiltrated, therefore it is commonly used in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. To discover and characterize the miRNAs and their cleaved target mRNAs in <italic>N. benthamiana</italic>, we sequenced small RNA transcriptomes and degradomes of two <italic>N. benthamiana</italic> accessions and validated them by Northern blots.</p></sec><sec><title>Results</title><p>We used a comprehensive molecular approach to detect and to experimentally validate <italic>N. benthamiana</italic> miRNAs and their target mRNAs from various tissues. We identified 40 conserved miRNA families and 18 novel microRNA candidates and validated their target mRNAs with a genomic scale approach. The accumulation of thirteen novel miRNAs was confirmed by Northern blot analysis. The conserved and novel miRNA targets were found to be involved in various biological processes including transcription, RNA binding, DNA modification, signal transduction, stress response and metabolic process. Among the novel miRNA targets we found the mRNA of REPRESSOR OF SILENCING (ROS1). Regulation of ROS1 by a miRNA provides a new regulatory layer to reinforce transcriptional gene silencing by a post-transcriptional repression of ROS1 activity.</p></sec><sec><title>Conclusions</title><p>The identified conserved and novel miRNAs along with their target mRNAs also provides a tissue specific atlas of known and new miRNA expression and their cleaved target mRNAs of <italic>N. benthamiana</italic>. Thus this study will serve as a valuable resource to the plant research community that will be beneficial well into the future.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12864-015-2209-6) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bombarely, A" uniqKey="Bombarely A">A Bombarely</name></author><author><name sortKey="Rosli, Hg" uniqKey="Rosli H">HG Rosli</name></author><author><name sortKey="Vrebalov, J" uniqKey="Vrebalov J">J Vrebalov</name></author><author><name sortKey="Moffett, P" uniqKey="Moffett P">P Moffett</name></author><author><name sortKey="Mueller, La" uniqKey="Mueller L">LA Mueller</name></author><author><name sortKey="Martin, Gb" uniqKey="Martin G">GB Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goodin, Mm" uniqKey="Goodin M">MM Goodin</name></author><author><name sortKey="Zaitlin, D" uniqKey="Zaitlin D">D Zaitlin</name></author><author><name sortKey="Naidu, Ra" uniqKey="Naidu R">RA Naidu</name></author><author><name sortKey="Lommel, Sa" uniqKey="Lommel S">SA Lommel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Silhavy, D" uniqKey="Silhavy D">D Silhavy</name></author><author><name sortKey="Burgyan, J" uniqKey="Burgyan J">J Burgyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petre, B" uniqKey="Petre B">B Petre</name></author><author><name sortKey="Saunders, Dg" uniqKey="Saunders D">DG Saunders</name></author><author><name sortKey="Sklenar, J" uniqKey="Sklenar J">J Sklenar</name></author><author><name sortKey="Lorrain, C" uniqKey="Lorrain C">C Lorrain</name></author><author><name sortKey="Win, J" uniqKey="Win J">J Win</name></author><author><name sortKey="Duplessis, S" uniqKey="Duplessis S">S Duplessis</name></author><author><name sortKey="Kamoun, S" uniqKey="Kamoun S">S Kamoun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nyiko, T" uniqKey="Nyiko T">T Nyiko</name></author><author><name sortKey="Kerenyi, F" uniqKey="Kerenyi F">F Kerenyi</name></author><author><name sortKey="Szabadkai, L" uniqKey="Szabadkai L">L Szabadkai</name></author><author><name sortKey="Benkovics, Ah" uniqKey="Benkovics A">AH Benkovics</name></author><author><name sortKey="Major, P" uniqKey="Major P">P Major</name></author><author><name sortKey="Sonkoly, B" uniqKey="Sonkoly B">B Sonkoly</name></author><author><name sortKey="Merai, Z" uniqKey="Merai Z">Z Merai</name></author><author><name sortKey="Barta, E" uniqKey="Barta E">E Barta</name></author><author><name sortKey="Niemiec, E" uniqKey="Niemiec E">E Niemiec</name></author><author><name sortKey="Kufel, J" uniqKey="Kufel J">J Kufel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Pan, S" uniqKey="Pan S">S Pan</name></author><author><name sortKey="Cheng, S" uniqKey="Cheng S">S Cheng</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author><author><name sortKey="Mu, D" uniqKey="Mu D">D Mu</name></author><author><name sortKey="Ni, P" uniqKey="Ni P">P Ni</name></author><author><name sortKey="Zhang, G" uniqKey="Zhang G">G Zhang</name></author><author><name sortKey="Yang, S" uniqKey="Yang S">S Yang</name></author><author><name sortKey="Li, R" uniqKey="Li R">R Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, S" uniqKey="Kim S">S Kim</name></author><author><name sortKey="Park, M" uniqKey="Park M">M Park</name></author><author><name sortKey="Yeom, Si" uniqKey="Yeom S">SI Yeom</name></author><author><name sortKey="Kim, Ym" uniqKey="Kim Y">YM Kim</name></author><author><name sortKey="Lee, Jm" uniqKey="Lee J">JM Lee</name></author><author><name sortKey="Lee, Ha" uniqKey="Lee H">HA Lee</name></author><author><name sortKey="Seo, E" uniqKey="Seo E">E Seo</name></author><author><name sortKey="Choi, J" uniqKey="Choi J">J Choi</name></author><author><name sortKey="Cheong, K" uniqKey="Cheong K">K Cheong</name></author><author><name sortKey="Kim, Kt" uniqKey="Kim K">KT Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sato, S" uniqKey="Sato S">S Sato</name></author><author><name sortKey="Tabata, S" uniqKey="Tabata S">S Tabata</name></author><author><name sortKey="Hirakawa, H" uniqKey="Hirakawa H">H Hirakawa</name></author><author><name sortKey="Asamizu, E" uniqKey="Asamizu E">E Asamizu</name></author><author><name sortKey="Shirasawa, K" uniqKey="Shirasawa K">K Shirasawa</name></author><author><name sortKey="Isobe, S" uniqKey="Isobe S">S Isobe</name></author><author><name sortKey="Kaneko, T" uniqKey="Kaneko T">T Kaneko</name></author><author><name sortKey="Nakamura, Y" uniqKey="Nakamura Y">Y Nakamura</name></author><author><name sortKey="Shibata, D" uniqKey="Shibata D">D Shibata</name></author><author><name sortKey="Aoki, K" uniqKey="Aoki K">K Aoki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qin, C" uniqKey="Qin C">C Qin</name></author><author><name sortKey="Yu, Cs" uniqKey="Yu C">CS Yu</name></author><author><name sortKey="Shen, Yo" uniqKey="Shen Y">YO Shen</name></author><author><name sortKey="Fang, Xd" uniqKey="Fang X">XD Fang</name></author><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Min, Jm" uniqKey="Min J">JM Min</name></author><author><name sortKey="Cheng, Jw" uniqKey="Cheng J">JW Cheng</name></author><author><name sortKey="Zhao, Sc" uniqKey="Zhao S">SC Zhao</name></author><author><name sortKey="Xu, M" uniqKey="Xu M">M Xu</name></author><author><name sortKey="Luo, Y" uniqKey="Luo Y">Y Luo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sierro, N" uniqKey="Sierro N">N Sierro</name></author><author><name sortKey="Battey, Jn" uniqKey="Battey J">JN Battey</name></author><author><name sortKey="Ouadi, S" uniqKey="Ouadi S">S Ouadi</name></author><author><name sortKey="Bovet, L" uniqKey="Bovet L">L Bovet</name></author><author><name sortKey="Goepfert, S" uniqKey="Goepfert S">S Goepfert</name></author><author><name sortKey="Bakaher, N" uniqKey="Bakaher N">N Bakaher</name></author><author><name sortKey="Peitsch, Mc" uniqKey="Peitsch M">MC Peitsch</name></author><author><name sortKey="Ivanov, Nv" uniqKey="Ivanov N">NV Ivanov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Burgyan, J" uniqKey="Burgyan J">J Burgyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rogers, K" uniqKey="Rogers K">K Rogers</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, Gl" uniqKey="Sun G">GL Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Li, Yf" uniqKey="Li Y">YF Li</name></author><author><name sortKey="Jagadeeswaran, G" uniqKey="Jagadeeswaran G">G Jagadeeswaran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chavez Montes, Ra" uniqKey="Chavez Montes R">RA Chavez Montes</name></author><author><name sortKey="De Fatima, R Cf" uniqKey="De Fatima R">R-CF de Fatima</name></author><author><name sortKey="De Paoli, E" uniqKey="De Paoli E">E De Paoli</name></author><author><name sortKey="Accerbi, M" uniqKey="Accerbi M">M Accerbi</name></author><author><name sortKey="Rymarquis, La" uniqKey="Rymarquis L">LA Rymarquis</name></author><author><name sortKey="Mahalingam, G" uniqKey="Mahalingam G">G Mahalingam</name></author><author><name sortKey="Marsch Martinez, N" uniqKey="Marsch Martinez N">N Marsch-Martinez</name></author><author><name sortKey="Meyers, Bc" uniqKey="Meyers B">BC Meyers</name></author><author><name sortKey="Green, Pj" uniqKey="Green P">PJ Green</name></author><author><name sortKey="De Folter, S" uniqKey="De Folter S">S de Folter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cuperus, Jt" uniqKey="Cuperus J">JT Cuperus</name></author><author><name sortKey="Fahlgren, N" uniqKey="Fahlgren N">N Fahlgren</name></author><author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author><author><name sortKey="Pan, X" uniqKey="Pan X">X Pan</name></author><author><name sortKey="Cannon, Ch" uniqKey="Cannon C">CH Cannon</name></author><author><name sortKey="Cobb, Gp" uniqKey="Cobb G">GP Cobb</name></author><author><name sortKey="Anderson, Ta" uniqKey="Anderson T">TA Anderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Naim, F" uniqKey="Naim F">F Naim</name></author><author><name sortKey="Nakasugi, K" uniqKey="Nakasugi K">K Nakasugi</name></author><author><name sortKey="Crowhurst, Rn" uniqKey="Crowhurst R">RN Crowhurst</name></author><author><name sortKey="Hilario, E" uniqKey="Hilario E">E Hilario</name></author><author><name sortKey="Zwart, Ab" uniqKey="Zwart A">AB Zwart</name></author><author><name sortKey="Hellens, Rp" uniqKey="Hellens R">RP Hellens</name></author><author><name sortKey="Taylor, Jm" uniqKey="Taylor J">JM Taylor</name></author><author><name sortKey="Waterhouse, Pm" uniqKey="Waterhouse P">PM Waterhouse</name></author><author><name sortKey="Wood, Cc" uniqKey="Wood C">CC Wood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nakasugi, K" uniqKey="Nakasugi K">K Nakasugi</name></author><author><name sortKey="Crowhurst, Rn" uniqKey="Crowhurst R">RN Crowhurst</name></author><author><name sortKey="Bally, J" uniqKey="Bally J">J Bally</name></author><author><name sortKey="Wood, Cc" uniqKey="Wood C">CC Wood</name></author><author><name sortKey="Hellens, Rp" uniqKey="Hellens R">RP Hellens</name></author><author><name sortKey="Waterhouse, Pm" uniqKey="Waterhouse P">PM Waterhouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nakasugi, K" uniqKey="Nakasugi K">K Nakasugi</name></author><author><name sortKey="Crowhurst, R" uniqKey="Crowhurst R">R Crowhurst</name></author><author><name sortKey="Bally, J" uniqKey="Bally J">J Bally</name></author><author><name sortKey="Waterhouse, P" uniqKey="Waterhouse P">P Waterhouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kawashima, Cg" uniqKey="Kawashima C">CG Kawashima</name></author><author><name sortKey="Yoshimoto, N" uniqKey="Yoshimoto N">N Yoshimoto</name></author><author><name sortKey="Maruyama Nakashita, A" uniqKey="Maruyama Nakashita A">A Maruyama-Nakashita</name></author><author><name sortKey="Tsuchiya, Yn" uniqKey="Tsuchiya Y">YN Tsuchiya</name></author><author><name sortKey="Saito, K" uniqKey="Saito K">K Saito</name></author><author><name sortKey="Takahashi, H" uniqKey="Takahashi H">H Takahashi</name></author><author><name sortKey="Dalmay, T" uniqKey="Dalmay T">T Dalmay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fujii, H" uniqKey="Fujii H">H Fujii</name></author><author><name sortKey="Chiou, Tj" uniqKey="Chiou T">TJ Chiou</name></author><author><name sortKey="Lin, Si" uniqKey="Lin S">SI Lin</name></author><author><name sortKey="Aung, K" uniqKey="Aung K">K Aung</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Pignatta, D" uniqKey="Pignatta D">D Pignatta</name></author><author><name sortKey="Bendix, C" uniqKey="Bendix C">C Bendix</name></author><author><name sortKey="Brunkard, Jo" uniqKey="Brunkard J">JO Brunkard</name></author><author><name sortKey="Cohn, Mm" uniqKey="Cohn M">MM Cohn</name></author><author><name sortKey="Tung, J" uniqKey="Tung J">J Tung</name></author><author><name sortKey="Sun, H" uniqKey="Sun H">H Sun</name></author><author><name sortKey="Kumar, P" uniqKey="Kumar P">P Kumar</name></author><author><name sortKey="Baker, B" uniqKey="Baker B">B Baker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, S" uniqKey="Tang S">S Tang</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z Li</name></author><author><name sortKey="Gui, Y" uniqKey="Gui Y">Y Gui</name></author><author><name sortKey="Xiao, B" uniqKey="Xiao B">B Xiao</name></author><author><name sortKey="Xie, J" uniqKey="Xie J">J Xie</name></author><author><name sortKey="Zhu, Qh" uniqKey="Zhu Q">QH Zhu</name></author><author><name sortKey="Fan, L" uniqKey="Fan L">L Fan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Hm" uniqKey="Chen H">HM Chen</name></author><author><name sortKey="Chen, Lt" uniqKey="Chen L">LT Chen</name></author><author><name sortKey="Patel, K" uniqKey="Patel K">K Patel</name></author><author><name sortKey="Li, Yh" uniqKey="Li Y">YH Li</name></author><author><name sortKey="Baulcombe, Dc" uniqKey="Baulcombe D">DC Baulcombe</name></author><author><name sortKey="Wu, Sh" uniqKey="Wu S">SH Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cuperus, Jt" uniqKey="Cuperus J">JT Cuperus</name></author><author><name sortKey="Carbonell, A" uniqKey="Carbonell A">A Carbonell</name></author><author><name sortKey="Fahlgren, N" uniqKey="Fahlgren N">N Fahlgren</name></author><author><name sortKey="Garcia Ruiz, H" uniqKey="Garcia Ruiz H">H Garcia-Ruiz</name></author><author><name sortKey="Burke, Rt" uniqKey="Burke R">RT Burke</name></author><author><name sortKey="Takeda, A" uniqKey="Takeda A">A Takeda</name></author><author><name sortKey="Sullivan, Cm" uniqKey="Sullivan C">CM Sullivan</name></author><author><name sortKey="Gilbert, Sd" uniqKey="Gilbert S">SD Gilbert</name></author><author><name sortKey="Montgomery, Ta" uniqKey="Montgomery T">TA Montgomery</name></author><author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shivaprasad, Pv" uniqKey="Shivaprasad P">PV Shivaprasad</name></author><author><name sortKey="Chen, Hm" uniqKey="Chen H">HM Chen</name></author><author><name sortKey="Patel, K" uniqKey="Patel K">K Patel</name></author><author><name sortKey="Bond, Dm" uniqKey="Bond D">DM Bond</name></author><author><name sortKey="Santos, Ba" uniqKey="Santos B">BA Santos</name></author><author><name sortKey="Baulcombe, Dc" uniqKey="Baulcombe D">DC Baulcombe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhai, Jx" uniqKey="Zhai J">JX Zhai</name></author><author><name sortKey="Jeong, Dh" uniqKey="Jeong D">DH Jeong</name></author><author><name sortKey="De Paoli, E" uniqKey="De Paoli E">E De Paoli</name></author><author><name sortKey="Park, S" uniqKey="Park S">S Park</name></author><author><name sortKey="Rosen, Bd" uniqKey="Rosen B">BD Rosen</name></author><author><name sortKey="Li, Yp" uniqKey="Li Y">YP Li</name></author><author><name sortKey="Gonzalez, Aj" uniqKey="Gonzalez A">AJ Gonzalez</name></author><author><name sortKey="Yan, Z" uniqKey="Yan Z">Z Yan</name></author><author><name sortKey="Kitto, Sl" uniqKey="Kitto S">SL Kitto</name></author><author><name sortKey="Grusak, Ma" uniqKey="Grusak M">MA Grusak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Voinnet, O" uniqKey="Voinnet O">O Voinnet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mi, Sj" uniqKey="Mi S">SJ Mi</name></author><author><name sortKey="Cai, T" uniqKey="Cai T">T Cai</name></author><author><name sortKey="Hu, Yg" uniqKey="Hu Y">YG Hu</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Hodges, E" uniqKey="Hodges E">E Hodges</name></author><author><name sortKey="Ni, Fr" uniqKey="Ni F">FR Ni</name></author><author><name sortKey="Wu, L" uniqKey="Wu L">L Wu</name></author><author><name sortKey="Li, S" uniqKey="Li S">S Li</name></author><author><name sortKey="Zhou, H" uniqKey="Zhou H">H Zhou</name></author><author><name sortKey="Long, Cz" uniqKey="Long C">CZ Long</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hafner, M" uniqKey="Hafner M">M Hafner</name></author><author><name sortKey="Renwick, N" uniqKey="Renwick N">N Renwick</name></author><author><name sortKey="Brown, M" uniqKey="Brown M">M Brown</name></author><author><name sortKey="Mihailovic, A" uniqKey="Mihailovic A">A Mihailovic</name></author><author><name sortKey="Holoch, D" uniqKey="Holoch D">D Holoch</name></author><author><name sortKey="Lin, C" uniqKey="Lin C">C Lin</name></author><author><name sortKey="Pena, Jt" uniqKey="Pena J">JT Pena</name></author><author><name sortKey="Nusbaum, Jd" uniqKey="Nusbaum J">JD Nusbaum</name></author><author><name sortKey="Morozov, P" uniqKey="Morozov P">P Morozov</name></author><author><name sortKey="Ludwig, J" uniqKey="Ludwig J">J Ludwig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sorefan, K" uniqKey="Sorefan K">K Sorefan</name></author><author><name sortKey="Pais, H" uniqKey="Pais H">H Pais</name></author><author><name sortKey="Hall, Ae" uniqKey="Hall A">AE Hall</name></author><author><name sortKey="Kozomara, A" uniqKey="Kozomara A">A Kozomara</name></author><author><name sortKey="Griffiths Jones, S" uniqKey="Griffiths Jones S">S Griffiths-Jones</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author><author><name sortKey="Dalmay, T" uniqKey="Dalmay T">T Dalmay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, Gh" uniqKey="Sun G">GH Sun</name></author><author><name sortKey="Wu, Xw" uniqKey="Wu X">XW Wu</name></author><author><name sortKey="Wang, Jh" uniqKey="Wang J">JH Wang</name></author><author><name sortKey="Li, Hq" uniqKey="Li H">HQ Li</name></author><author><name sortKey="Li, Xj" uniqKey="Li X">XJ Li</name></author><author><name sortKey="Gao, Hl" uniqKey="Gao H">HL Gao</name></author><author><name sortKey="Rossi, J" uniqKey="Rossi J">J Rossi</name></author><author><name sortKey="Yen, Y" uniqKey="Yen Y">Y Yen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Pantaleo, V" uniqKey="Pantaleo V">V Pantaleo</name></author><author><name sortKey="Toth, G" uniqKey="Toth G">G Toth</name></author><author><name sortKey="Rusholme Pilcher, Rl" uniqKey="Rusholme Pilcher R">RL Rusholme Pilcher</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author><author><name sortKey="Burgyan, J" uniqKey="Burgyan J">J Burgyan</name></author><author><name sortKey="Dalmay, T" uniqKey="Dalmay T">T Dalmay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Jing, R" uniqKey="Jing R">R Jing</name></author><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Schwach, F" uniqKey="Schwach F">F Schwach</name></author><author><name sortKey="Rusholme Pilcher, Rl" uniqKey="Rusholme Pilcher R">RL Rusholme Pilcher</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author><author><name sortKey="Dalmay, T" uniqKey="Dalmay T">T Dalmay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pantaleo, V" uniqKey="Pantaleo V">V Pantaleo</name></author><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Miozzi, L" uniqKey="Miozzi L">L Miozzi</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author><author><name sortKey="Dalmay, T" uniqKey="Dalmay T">T Dalmay</name></author><author><name sortKey="Burgyan, J" uniqKey="Burgyan J">J Burgyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="German, Ma" uniqKey="German M">MA German</name></author><author><name sortKey="Luo, S" uniqKey="Luo S">S Luo</name></author><author><name sortKey="Schroth, G" uniqKey="Schroth G">G Schroth</name></author><author><name sortKey="Meyers, Bc" uniqKey="Meyers B">BC Meyers</name></author><author><name sortKey="Green, Pj" uniqKey="Green P">PJ Green</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Addo Quaye, C" uniqKey="Addo Quaye C">C Addo-Quaye</name></author><author><name sortKey="Eshoo, Tw" uniqKey="Eshoo T">TW Eshoo</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="German, Ma" uniqKey="German M">MA German</name></author><author><name sortKey="Pillay, M" uniqKey="Pillay M">M Pillay</name></author><author><name sortKey="Jeong, Dh" uniqKey="Jeong D">DH Jeong</name></author><author><name sortKey="Hetawal, A" uniqKey="Hetawal A">A Hetawal</name></author><author><name sortKey="Luo, S" uniqKey="Luo S">S Luo</name></author><author><name sortKey="Janardhanan, P" uniqKey="Janardhanan P">P Janardhanan</name></author><author><name sortKey="Kannan, V" uniqKey="Kannan V">V Kannan</name></author><author><name sortKey="Rymarquis, La" uniqKey="Rymarquis L">LA Rymarquis</name></author><author><name sortKey="Nobuta, K" uniqKey="Nobuta K">K Nobuta</name></author><author><name sortKey="German, R" uniqKey="German R">R German</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Folkes, L" uniqKey="Folkes L">L Folkes</name></author><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Woolfenden, Hc" uniqKey="Woolfenden H">HC Woolfenden</name></author><author><name sortKey="Stocks, Mb" uniqKey="Stocks M">MB Stocks</name></author><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Dalmay, T" uniqKey="Dalmay T">T Dalmay</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Addo Quaye, C" uniqKey="Addo Quaye C">C Addo-Quaye</name></author><author><name sortKey="Miller, W" uniqKey="Miller W">W Miller</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Itaya, A" uniqKey="Itaya A">A Itaya</name></author><author><name sortKey="Zhong, Xh" uniqKey="Zhong X">XH Zhong</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name></author><author><name sortKey="Zhang, Jf" uniqKey="Zhang J">JF Zhang</name></author><author><name sortKey="Van Der Knaap, E" uniqKey="Van Der Knaap E">E van der Knaap</name></author><author><name sortKey="Olmstead, R" uniqKey="Olmstead R">R Olmstead</name></author><author><name sortKey="Qi, Yj" uniqKey="Qi Y">YJ Qi</name></author><author><name sortKey="Ding, Ba" uniqKey="Ding B">BA Ding</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pandey, Sp" uniqKey="Pandey S">SP Pandey</name></author><author><name sortKey="Shahi, P" uniqKey="Shahi P">P Shahi</name></author><author><name sortKey="Gase, K" uniqKey="Gase K">K Gase</name></author><author><name sortKey="Baldwin, It" uniqKey="Baldwin I">IT Baldwin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, H" uniqKey="Zhang H">H Zhang</name></author><author><name sortKey="Gao, Z" uniqKey="Gao Z">Z Gao</name></author><author><name sortKey="Zheng, X" uniqKey="Zheng X">X Zheng</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thung, L" uniqKey="Thung L">L Thung</name></author><author><name sortKey="Trusov, Y" uniqKey="Trusov Y">Y Trusov</name></author><author><name sortKey="Chakravorty, D" uniqKey="Chakravorty D">D Chakravorty</name></author><author><name sortKey="Botella, Jr" uniqKey="Botella J">JR Botella</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Urano, D" uniqKey="Urano D">D Urano</name></author><author><name sortKey="Chen, Jg" uniqKey="Chen J">JG Chen</name></author><author><name sortKey="Botella, Jr" uniqKey="Botella J">JR Botella</name></author><author><name sortKey="Jones, Am" uniqKey="Jones A">AM Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rasheed, Sa" uniqKey="Rasheed S">SA Rasheed</name></author><author><name sortKey="Teo, Cr" uniqKey="Teo C">CR Teo</name></author><author><name sortKey="Beillard, Ej" uniqKey="Beillard E">EJ Beillard</name></author><author><name sortKey="Voorhoeve, Pm" uniqKey="Voorhoeve P">PM Voorhoeve</name></author><author><name sortKey="Zhou, W" uniqKey="Zhou W">W Zhou</name></author><author><name sortKey="Ghosh, S" uniqKey="Ghosh S">S Ghosh</name></author><author><name sortKey="Casey, Pj" uniqKey="Casey P">PJ Casey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, J" uniqKey="Hu J">J Hu</name></author><author><name sortKey="Baker, A" uniqKey="Baker A">A Baker</name></author><author><name sortKey="Bartel, B" uniqKey="Bartel B">B Bartel</name></author><author><name sortKey="Linka, N" uniqKey="Linka N">N Linka</name></author><author><name sortKey="Mullen, Rt" uniqKey="Mullen R">RT Mullen</name></author><author><name sortKey="Reumann, S" uniqKey="Reumann S">S Reumann</name></author><author><name sortKey="Zolman, Bk" uniqKey="Zolman B">BK Zolman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schumann, U" uniqKey="Schumann U">U Schumann</name></author><author><name sortKey="Wanner, G" uniqKey="Wanner G">G Wanner</name></author><author><name sortKey="Veenhuis, M" uniqKey="Veenhuis M">M Veenhuis</name></author><author><name sortKey="Schmid, M" uniqKey="Schmid M">M Schmid</name></author><author><name sortKey="Gietl, C" uniqKey="Gietl C">C Gietl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kamigaki, A" uniqKey="Kamigaki A">A Kamigaki</name></author><author><name sortKey="Kondo, M" uniqKey="Kondo M">M Kondo</name></author><author><name sortKey="Mano, S" uniqKey="Mano S">S Mano</name></author><author><name sortKey="Hayashi, M" uniqKey="Hayashi M">M Hayashi</name></author><author><name sortKey="Nishimura, M" uniqKey="Nishimura M">M Nishimura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schumann, U" uniqKey="Schumann U">U Schumann</name></author><author><name sortKey="Prestele, J" uniqKey="Prestele J">J Prestele</name></author><author><name sortKey="O Een, H" uniqKey="O Een H">H O’Geen</name></author><author><name sortKey="Brueggeman, R" uniqKey="Brueggeman R">R Brueggeman</name></author><author><name sortKey="Wanner, G" uniqKey="Wanner G">G Wanner</name></author><author><name sortKey="Gietl, C" uniqKey="Gietl C">C Gietl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morales Ruiz, T" uniqKey="Morales Ruiz T">T Morales-Ruiz</name></author><author><name sortKey="Ortega Galisteo, Ap" uniqKey="Ortega Galisteo A">AP Ortega-Galisteo</name></author><author><name sortKey="Ponferrada Marin, Mi" uniqKey="Ponferrada Marin M">MI Ponferrada-Marin</name></author><author><name sortKey="Martinez Macias, Mi" uniqKey="Martinez Macias M">MI Martinez-Macias</name></author><author><name sortKey="Ariza, Rr" uniqKey="Ariza R">RR Ariza</name></author><author><name sortKey="Roldan Arjona, T" uniqKey="Roldan Arjona T">T Roldan-Arjona</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Law, Ja" uniqKey="Law J">JA Law</name></author><author><name sortKey="Jacobsen, Se" uniqKey="Jacobsen S">SE Jacobsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Sun, H" uniqKey="Sun H">H Sun</name></author><author><name sortKey="Zeng, J" uniqKey="Zeng J">J Zeng</name></author><author><name sortKey="Cao, Z" uniqKey="Cao Z">Z Cao</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Qian, W" uniqKey="Qian W">W Qian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, Bp" uniqKey="Williams B">BP Williams</name></author><author><name sortKey="Pignatta, D" uniqKey="Pignatta D">D Pignatta</name></author><author><name sortKey="Henikoff, S" uniqKey="Henikoff S">S Henikoff</name></author><author><name sortKey="Gehring, M" uniqKey="Gehring M">M Gehring</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Jy" uniqKey="Kim J">JY Kim</name></author><author><name sortKey="Kwak, Kj" uniqKey="Kwak K">KJ Kwak</name></author><author><name sortKey="Jung, Hj" uniqKey="Jung H">HJ Jung</name></author><author><name sortKey="Lee, Hj" uniqKey="Lee H">HJ Lee</name></author><author><name sortKey="Kang, H" uniqKey="Kang H">H Kang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, S" uniqKey="Li S">S Li</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X Zhou</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Zhu, L" uniqKey="Zhu L">L Zhu</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S Zhang</name></author><author><name sortKey="Zhao, Y" uniqKey="Zhao Y">Y Zhao</name></author><author><name sortKey="Guo, J" uniqKey="Guo J">J Guo</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Chen, R" uniqKey="Chen R">R Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paul, S" uniqKey="Paul S">S Paul</name></author><author><name sortKey="Datta, Sk" uniqKey="Datta S">SK Datta</name></author><author><name sortKey="Datta, K" uniqKey="Datta K">K Datta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Salamon, P" uniqKey="Salamon P">P Salamon</name></author><author><name sortKey="Burgyan, J" uniqKey="Burgyan J">J Burgyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pall, Gs" uniqKey="Pall G">GS Pall</name></author><author><name sortKey="Hamilton, Aj" uniqKey="Hamilton A">AJ Hamilton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name></author><author><name sortKey="Ridzon, Da" uniqKey="Ridzon D">DA Ridzon</name></author><author><name sortKey="Broomer, Aj" uniqKey="Broomer A">AJ Broomer</name></author><author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z Zhou</name></author><author><name sortKey="Lee, Dh" uniqKey="Lee D">DH Lee</name></author><author><name sortKey="Nguyen, Jt" uniqKey="Nguyen J">JT Nguyen</name></author><author><name sortKey="Barbisin, M" uniqKey="Barbisin M">M Barbisin</name></author><author><name sortKey="Xu, Nl" uniqKey="Xu N">NL Xu</name></author><author><name sortKey="Mahuvakar, Vr" uniqKey="Mahuvakar V">VR Mahuvakar</name></author><author><name sortKey="Andersen, Mr" uniqKey="Andersen M">MR Andersen</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Varkonyi Gasic, E" uniqKey="Varkonyi Gasic E">E Varkonyi-Gasic</name></author><author><name sortKey="Wu, R" uniqKey="Wu R">R Wu</name></author><author><name sortKey="Wood, M" uniqKey="Wood M">M Wood</name></author><author><name sortKey="Walton, Ef" uniqKey="Walton E">EF Walton</name></author><author><name sortKey="Hellens, Rp" uniqKey="Hellens R">RP Hellens</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Stocks, Mb" uniqKey="Stocks M">MB Stocks</name></author><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Mapleson, D" uniqKey="Mapleson D">D Mapleson</name></author><author><name sortKey="Woolfenden, Hc" uniqKey="Woolfenden H">HC Woolfenden</name></author><author><name sortKey="Mohorianu, I" uniqKey="Mohorianu I">I Mohorianu</name></author><author><name sortKey="Folkes, L" uniqKey="Folkes L">L Folkes</name></author><author><name sortKey="Schwach, F" uniqKey="Schwach F">F Schwach</name></author><author><name sortKey="Dalmay, T" uniqKey="Dalmay T">T Dalmay</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Grabherr, Mg" uniqKey="Grabherr M">MG Grabherr</name></author><author><name sortKey="Haas, Bj" uniqKey="Haas B">BJ Haas</name></author><author><name sortKey="Yassour, M" uniqKey="Yassour M">M Yassour</name></author><author><name sortKey="Levin, Jz" uniqKey="Levin J">JZ Levin</name></author><author><name sortKey="Thompson, Da" uniqKey="Thompson D">DA Thompson</name></author><author><name sortKey="Amit, I" uniqKey="Amit I">I Amit</name></author><author><name sortKey="Adiconis, X" uniqKey="Adiconis X">X Adiconis</name></author><author><name sortKey="Fan, L" uniqKey="Fan L">L Fan</name></author><author><name sortKey="Raychowdhury, R" uniqKey="Raychowdhury R">R Raychowdhury</name></author><author><name sortKey="Zeng, Qd" uniqKey="Zeng Q">QD Zeng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, Z" uniqKey="Du Z">Z Du</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X Zhou</name></author><author><name sortKey="Ling, Y" uniqKey="Ling Y">Y Ling</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Su, Z" uniqKey="Su Z">Z Su</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Genomics</journal-id><journal-id journal-id-type="iso-abbrev">BMC Genomics</journal-id><journal-title-group><journal-title>BMC Genomics</journal-title></journal-title-group><issn pub-type="epub">1471-2164</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26626050</article-id><article-id pub-id-type="pmc">4667520</article-id><article-id pub-id-type="publisher-id">2209</article-id><article-id pub-id-type="doi">10.1186/s12864-015-2209-6</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Identification of <italic>Nicotiana benthamiana</italic> microRNAs and their targets using high throughput sequencing and degradome analysis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Baksa</surname><given-names>Ivett</given-names></name><address><email>ivett.baksa@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Nagy</surname><given-names>Tibor</given-names></name><address><email>black007@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Barta</surname><given-names>Endre</given-names></name><address><email>barta@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Havelda</surname><given-names>Zoltán</given-names></name><address><email>havelda@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Várallyay</surname><given-names>Éva</given-names></name><address><email>varallya@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Silhavy</surname><given-names>Dániel</given-names></name><address><email>silhavy@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Burgyán</surname><given-names>József</given-names></name><address><email>burgyan@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Szittya</surname><given-names>György</given-names></name><address><phone>+36-28-526230</phone><email>szittya@abc.hu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1">Institute of Plant Biotechnology, National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent-Györgyi Albert ut 4, H-2100 Gödöllő, Hungary</aff></contrib-group><pub-date pub-type="epub"><day>1</day><month>12</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>12</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>16</volume><elocation-id>1025</elocation-id><history><date date-type="received"><day>23</day><month>7</month><year>2015</year></date><date date-type="accepted"><day>12</day><month>11</month><year>2015</year></date></history><permissions><copyright-statement>© Baksa et al. 2015</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p><italic>Nicotiana benthamiana</italic> is a widely used model plant species for research on plant-pathogen interactions as well as other areas of plant science. It can be easily transformed or agroinfiltrated, therefore it is commonly used in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. To discover and characterize the miRNAs and their cleaved target mRNAs in <italic>N. benthamiana</italic>, we sequenced small RNA transcriptomes and degradomes of two <italic>N. benthamiana</italic> accessions and validated them by Northern blots.</p></sec><sec><title>Results</title><p>We used a comprehensive molecular approach to detect and to experimentally validate <italic>N. benthamiana</italic> miRNAs and their target mRNAs from various tissues. We identified 40 conserved miRNA families and 18 novel microRNA candidates and validated their target mRNAs with a genomic scale approach. The accumulation of thirteen novel miRNAs was confirmed by Northern blot analysis. The conserved and novel miRNA targets were found to be involved in various biological processes including transcription, RNA binding, DNA modification, signal transduction, stress response and metabolic process. Among the novel miRNA targets we found the mRNA of REPRESSOR OF SILENCING (ROS1). Regulation of ROS1 by a miRNA provides a new regulatory layer to reinforce transcriptional gene silencing by a post-transcriptional repression of ROS1 activity.</p></sec><sec><title>Conclusions</title><p>The identified conserved and novel miRNAs along with their target mRNAs also provides a tissue specific atlas of known and new miRNA expression and their cleaved target mRNAs of <italic>N. benthamiana</italic>. Thus this study will serve as a valuable resource to the plant research community that will be beneficial well into the future.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12864-015-2209-6) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd><italic>Nicotiana benthamiana</italic></kwd><kwd>microRNA</kwd><kwd>Non-coding RNAs</kwd><kwd>Small RNA</kwd><kwd>Degradome</kwd><kwd>High throughput sequencing</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2015</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000141 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000141 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MelampsoraV2
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:4667520
|texte= Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:26626050" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MelampsoraV2
| This area was generated with Dilib version V0.6.38. |  |