MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina
Identifieur interne : 000142 ( Pmc/Curation ); précédent : 000141; suivant : 000143MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina
Auteurs : Danlei Li [République populaire de Chine] ; Feng Wang [République populaire de Chine] ; Chao Wang [République populaire de Chine] ; Li Zou [République populaire de Chine] ; Zhiying Wang [République populaire de Chine] ; Qiaoli Chen [République populaire de Chine] ; Chunyang Niu [République populaire de Chine] ; Ruizhi Zhang [République populaire de Chine] ; Yaming Ling [République populaire de Chine] ; Bowen Wang [République populaire de Chine]Source :
- BMC Genomics [ 1471-2164 ] ; 2016.
Abstract
Rust caused by
To study the susceptibility of poplar to
We found that the defense-related post-transcriptional regulation of the susceptible poplar ‘Robusta’ functions normally only at the stage of PAMPs and PAMP-triggered immunity (PTI). More importantly, the miRNA-mediated post-transcriptional regulation of defense signal pathway genes were inactivated by the infection of virulent rust at the stage of effector-triggered susceptibility and during the following stages of salicylic acid and hypersensitive responses. This inactivation was the major characteristic of ‘Robusta’ susceptibility.
The online version of this article (doi:10.1186/s12864-015-2286-6) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12864-015-2286-6
PubMed: 26768277
PubMed Central: 4714501
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Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Zou, Li" sort="Zou, Li" uniqKey="Zou L" first="Li" last="Zou">Li Zou</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Zhiying" sort="Wang, Zhiying" uniqKey="Wang Z" first="Zhiying" last="Wang">Zhiying Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Qiaoli" sort="Chen, Qiaoli" uniqKey="Chen Q" first="Qiaoli" last="Chen">Qiaoli Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Niu, Chunyang" sort="Niu, Chunyang" uniqKey="Niu C" first="Chunyang" last="Niu">Chunyang Niu</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Ruizhi" sort="Zhang, Ruizhi" uniqKey="Zhang R" first="Ruizhi" last="Zhang">Ruizhi Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Ling, Yaming" sort="Ling, Yaming" uniqKey="Ling Y" first="Yaming" last="Ling">Yaming Ling</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Bowen" sort="Wang, Bowen" uniqKey="Wang B" first="Bowen" last="Wang">Bowen Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26768277</idno><idno type="pmc">4714501</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714501</idno><idno type="RBID">PMC:4714501</idno><idno type="doi">10.1186/s12864-015-2286-6</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000142</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000142</idno><idno type="wicri:Area/Pmc/Curation">000142</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000142</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent <italic>Melampsora larici-populina</italic></title><author><name sortKey="Li, Danlei" sort="Li, Danlei" uniqKey="Li D" first="Danlei" last="Li">Danlei Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Feng" sort="Wang, Feng" uniqKey="Wang F" first="Feng" last="Wang">Feng Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Zou, Li" sort="Zou, Li" uniqKey="Zou L" first="Li" last="Zou">Li Zou</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Zhiying" sort="Wang, Zhiying" uniqKey="Wang Z" first="Zhiying" last="Wang">Zhiying Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Qiaoli" sort="Chen, Qiaoli" uniqKey="Chen Q" first="Qiaoli" last="Chen">Qiaoli Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Niu, Chunyang" sort="Niu, Chunyang" uniqKey="Niu C" first="Chunyang" last="Niu">Chunyang Niu</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Ruizhi" sort="Zhang, Ruizhi" uniqKey="Zhang R" first="Ruizhi" last="Zhang">Ruizhi Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Ling, Yaming" sort="Ling, Yaming" uniqKey="Ling Y" first="Yaming" last="Ling">Yaming Ling</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Bowen" sort="Wang, Bowen" uniqKey="Wang B" first="Bowen" last="Wang">Bowen Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Rust caused by <italic>Melampsora larici-populina</italic> is one of the most damaging diseases of poplars. Rust is considered to be a model pathogen for genetic studies because both pathogen and host genomes are available. The poplar ‘Robusta’, whose general rust resistance is defeated by virulent rust E4, provides suitable host material for studies of the gene regulation involved in rust resistance/susceptibility. In this study, we investigated the microRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent rust. We were particularly interested in delineating the host-pathogen interactions with a specific focus on microRNAs (miRNAs).</p></sec><sec><title>Results</title><p>To study the susceptibility of poplar to <italic>M. larici-populina</italic>, small RNA (sRNA) libraries, a degradome cDNA library and digital gene expression libraries were constructed for rust-inoculated and rust-free susceptible poplar ‘Robusta’ leaves through high-throughput sequencing. Altogether, 12,722 regulating interactions were identified. The results delineated the framework of post-transcriptional regulation of gene expression in the susceptible poplar, which was infected by the virulent rust. The results indicated that pathogen-associated molecular patterns (PAMPs) and PAMP-triggered immunity were induced by the infection of virulent rust E4 and that miRNAs still functioned at this stage. After this stage, miRNA-regulated <italic>R</italic> genes, such as TIR-NBS-LRR and CC-NBS-LRR, were not fully functional. Additionally, the rust-responsive miRNAs did not regulate the signaling component genes related to the salicylic acid pathway or the hypersensitive response.</p></sec><sec><title>Conclusions</title><p>We found that the defense-related post-transcriptional regulation of the susceptible poplar ‘Robusta’ functions normally only at the stage of PAMPs and PAMP-triggered immunity (PTI). More importantly, the miRNA-mediated post-transcriptional regulation of defense signal pathway genes were inactivated by the infection of virulent rust at the stage of effector-triggered susceptibility and during the following stages of salicylic acid and hypersensitive responses. This inactivation was the major characteristic of ‘Robusta’ susceptibility.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12864-015-2286-6) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Van Kraayenoord, Cws" uniqKey="Van Kraayenoord C">CWS Van Kraayenoord</name></author><author><name sortKey="Laundon, Gf" uniqKey="Laundon G">GF Laundon</name></author><author><name sortKey="Spiers, Ag" uniqKey="Spiers A">AG Spiers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Newcombe, G" uniqKey="Newcombe G">G Newcombe</name></author><author><name sortKey="Chastagner, Ga" uniqKey="Chastagner G">GA Chastagner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pei, Mh" uniqKey="Pei M">MH Pei</name></author><author><name sortKey="Shang, Yz" uniqKey="Shang Y">YZ Shang</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Bean, Wj" uniqKey="Bean W">WJ Bean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boyle, J" uniqKey="Boyle J">J Boyle</name></author><author><name sortKey="Winjum, J" uniqKey="Winjum J">J Winjum</name></author><author><name sortKey="Kavanagh, K" uniqKey="Kavanagh K">K Kavanagh</name></author><author><name sortKey="Jensen, E" uniqKey="Jensen E">E Jensen</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Steenackers, V" uniqKey="Steenackers V">V Steenackers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pinon, J" uniqKey="Pinon J">J Pinon</name></author><author><name sortKey="Van Dam, Bc" uniqKey="Van Dam B">BC van Dam</name></author><author><name sortKey="Genetet, I" uniqKey="Genetet I">I Genetet</name></author><author><name sortKey="De Kam, M" uniqKey="De Kam M">M De Kam</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Nancy, Ae" uniqKey="Nancy A">AE Nancy</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Baohong, Z" uniqKey="Baohong Z">Z Baohong</name></author><author><name sortKey="Xiaoping, P" uniqKey="Xiaoping P">P Xiaoping</name></author><author><name sortKey="Cobb, Gp" uniqKey="Cobb G">GP Cobb</name></author><author><name sortKey="Anderson, Ta" uniqKey="Anderson T">TA Anderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jones Rhoades, Mww" uniqKey="Jones Rhoades M">MWW Jones-Rhoades</name></author><author><name sortKey="Bartel, Dpp" uniqKey="Bartel D">DPP Bartel</name></author><author><name sortKey="Bartel, B" uniqKey="Bartel B">B Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jones Rhoades, Mww" uniqKey="Jones Rhoades M">MWW Jones-Rhoades</name></author><author><name sortKey="David, Pb" uniqKey="David P">PB David</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Padmanabhan, C" uniqKey="Padmanabhan C">C Padmanabhan</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fahlgren, N" uniqKey="Fahlgren N">N Fahlgren</name></author><author><name sortKey="Howell, Md" uniqKey="Howell M">MD Howell</name></author><author><name sortKey="Kasschau, Kd" uniqKey="Kasschau K">KD Kasschau</name></author><author><name sortKey="Chapman, Ej" uniqKey="Chapman E">EJ Chapman</name></author><author><name sortKey="Sullivan, Cm" uniqKey="Sullivan C">CM Sullivan</name></author><author><name sortKey="Cumbie, Js" uniqKey="Cumbie J">JS Cumbie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jagadeeswaran, G" uniqKey="Jagadeeswaran G">G Jagadeeswaran</name></author><author><name sortKey="Saini, A" uniqKey="Saini A">A Saini</name></author><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, Xf" uniqKey="He X">XF He</name></author><author><name sortKey="Fang, Yy" uniqKey="Fang Y">YY Fang</name></author><author><name sortKey="Feng, L" uniqKey="Feng L">L Feng</name></author><author><name sortKey="Guo, Hs" uniqKey="Guo H">HS Guo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, Jp" uniqKey="Zhao J">JP Zhao</name></author><author><name sortKey="Jiang, Xl" uniqKey="Jiang X">XL Jiang</name></author><author><name sortKey="Zhang, By" uniqKey="Zhang B">BY Zhang</name></author><author><name sortKey="Su, Xh" uniqKey="Su X">XH Su</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petre, B" uniqKey="Petre B">B Petre</name></author><author><name sortKey="Morin, E" uniqKey="Morin E">E Morin</name></author><author><name sortKey="Tisserant, E" uniqKey="Tisserant E">E Tisserant</name></author><author><name sortKey="Hacquard, S" uniqKey="Hacquard S">S Hacquard</name></author><author><name sortKey="Da Silva, C" uniqKey="Da Silva C">C Da Silva</name></author><author><name sortKey="Poulain, J" uniqKey="Poulain J">J Poulain</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olivier, V" uniqKey="Olivier V">V Olivier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hacquard, S" uniqKey="Hacquard S">S Hacquard</name></author><author><name sortKey="Delaruelle, C" uniqKey="Delaruelle C">C Delaruelle</name></author><author><name sortKey="Legue, V" uniqKey="Legue V">V Legué</name></author><author><name sortKey="Tisserant, E" uniqKey="Tisserant E">E Tisserant</name></author><author><name sortKey="Kohler, A" uniqKey="Kohler A">A Kohler</name></author><author><name sortKey="Frey, P" uniqKey="Frey P">P Frey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duplessis, S" uniqKey="Duplessis S">S Duplessis</name></author><author><name sortKey="Hacquard, S" uniqKey="Hacquard S">S Hacquard</name></author><author><name sortKey="Delaruelle, C" uniqKey="Delaruelle C">C Delaruelle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abdelali, B" uniqKey="Abdelali B">B Abdelali</name></author><author><name sortKey="Phillip, Kw" uniqKey="Phillip K">KW Phillip</name></author><author><name sortKey="Scott, Dl" uniqKey="Scott D">DL Scott</name></author><author><name sortKey="Claude, Wd" uniqKey="Claude W">WD Claude</name></author><author><name sortKey="John, Ec" uniqKey="John E">EC John</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vitantonio, P" uniqKey="Vitantonio P">P Vitantonio</name></author><author><name sortKey="Gyorgy, S" uniqKey="Gyorgy S">S Gyorgy</name></author><author><name sortKey="Simon, M" uniqKey="Simon M">M Simon</name></author><author><name sortKey="Laura, M" uniqKey="Laura M">M Laura</name></author><author><name sortKey="Vincent, M" uniqKey="Vincent M">M Vincent</name></author><author><name sortKey="Tamas, D" uniqKey="Tamas D">D Tamas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rajagopalan, R" uniqKey="Rajagopalan R">R Rajagopalan</name></author><author><name sortKey="Vaucheret, H" uniqKey="Vaucheret H">H Vaucheret</name></author><author><name sortKey="Trejo, J" uniqKey="Trejo J">J Trejo</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Jing, R" uniqKey="Jing R">R Jing</name></author><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Schwach, F" uniqKey="Schwach F">F Schwach</name></author><author><name sortKey="Rusholme, Prl" uniqKey="Rusholme P">PRL Rusholme</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jinghua, Y" uniqKey="Jinghua Y">Y Jinghua</name></author><author><name sortKey="Xunyan, L" uniqKey="Xunyan L">L Xunyan</name></author><author><name sortKey="Baochen, X" uniqKey="Baochen X">X Baochen</name></author><author><name sortKey="Na, Z" uniqKey="Na Z">Z Na</name></author><author><name sortKey="Xiaodong, Y" uniqKey="Xiaodong Y">Y Xiaodong</name></author><author><name sortKey="Mingfang, Z" uniqKey="Mingfang Z">Z Mingfang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simon, A" uniqKey="Simon A">A Simon</name></author><author><name sortKey="Wolfgang, H" uniqKey="Wolfgang H">H Wolfgang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="By Yoav, B" uniqKey="By Yoav B">B By Yoav</name></author><author><name sortKey="Daniel, Y" uniqKey="Daniel Y">Y Daniel</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Laurans, F" uniqKey="Laurans F">F Laurans</name></author><author><name sortKey="Pilate, G" uniqKey="Pilate G">G Pilate</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rinaldi, C" uniqKey="Rinaldi C">C Rinaldi</name></author><author><name sortKey="Kohler, A" uniqKey="Kohler A">A Kohler</name></author><author><name sortKey="Frey, P" uniqKey="Frey P">P Frey</name></author><author><name sortKey="Duchaussoy, F" uniqKey="Duchaussoy F">F Duchaussoy</name></author><author><name sortKey="Ningre, N" uniqKey="Ningre N">N Ningre</name></author><author><name sortKey="Couloux, A" uniqKey="Couloux A">A Couloux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hacquard, S" uniqKey="Hacquard S">S Hacquard</name></author><author><name sortKey="Veneault Fourrey, C" uniqKey="Veneault Fourrey C">C Veneault-Fourrey</name></author><author><name sortKey="Delaruelle, C" uniqKey="Delaruelle C">C Delaruelle</name></author><author><name sortKey="Frey, P" uniqKey="Frey P">P Frey</name></author><author><name sortKey="Martin, F" uniqKey="Martin F">F Martin</name></author><author><name sortKey="Duplessis, S" uniqKey="Duplessis S">S Duplessis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glazebrook, J" uniqKey="Glazebrook J">J Glazebrook</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nawrath, C" uniqKey="Nawrath C">C Nawrath</name></author><author><name sortKey="Metraux, Jp" uniqKey="Metraux J">JP Metraux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author><author><name sortKey="Dunoyer, P" uniqKey="Dunoyer P">P Dunoyer</name></author><author><name sortKey="Jay, F" uniqKey="Jay F">F Jay</name></author><author><name sortKey="Arnold, B" uniqKey="Arnold B">B Arnold</name></author><author><name sortKey="Dharmasiri, N" uniqKey="Dharmasiri N">N Dharmasiri</name></author><author><name sortKey="Estelle, M" uniqKey="Estelle M">M Estelle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monk, Ce" uniqKey="Monk C">CE Monk</name></author><author><name sortKey="Hutvagner, G" uniqKey="Hutvagner G">G Hutvagner</name></author><author><name sortKey="Arthur, Jsc" uniqKey="Arthur J">JSC Arthur</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Pt" uniqKey="Liu P">PT Liu</name></author><author><name sortKey="Wheelwright, M" uniqKey="Wheelwright M">M Wheelwright</name></author><author><name sortKey="Teles, R" uniqKey="Teles R">R Teles</name></author><author><name sortKey="Komisopoulou, E" uniqKey="Komisopoulou E">E Komisopoulou</name></author><author><name sortKey="Edfeldt, K" uniqKey="Edfeldt K">K Edfeldt</name></author><author><name sortKey="Ferguson, B" uniqKey="Ferguson B">B Ferguson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, F" uniqKey="Ma F">F Ma</name></author><author><name sortKey="Xu, S" uniqKey="Xu S">S Xu</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Liu, M" uniqKey="Liu M">M Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Om, Pg" uniqKey="Om P">PG Om</name></author><author><name sortKey="Pradeep, S" uniqKey="Pradeep S">S Pradeep</name></author><author><name sortKey="Raj, Kg" uniqKey="Raj K">KG Raj</name></author><author><name sortKey="Indu, S" uniqKey="Indu S">S Indu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jonathan, Dgj" uniqKey="Jonathan D">DGJ Jonathan</name></author><author><name sortKey="Jeffery, Ld" uniqKey="Jeffery L">LD Jeffery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dangl, Jl" uniqKey="Dangl J">JL Dangl</name></author><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Annegret, K" uniqKey="Annegret K">K Annegret</name></author><author><name sortKey="Cecile, R" uniqKey="Cecile R">R Cécile</name></author><author><name sortKey="Sebastien, D" uniqKey="Sebastien D">D Sébastien</name></author><author><name sortKey="Marie, B" uniqKey="Marie B">B Marie</name></author><author><name sortKey="Danny, G" uniqKey="Danny G">G Danny</name></author><author><name sortKey="Frederic, D" uniqKey="Frederic D">D Frédéric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Padubidri, Vs" uniqKey="Padubidri V">VS Padubidri</name></author><author><name sortKey="Ho Ming, C" uniqKey="Ho Ming C">C Ho-Ming</name></author><author><name sortKey="Kanu, P" uniqKey="Kanu P">P Kanu</name></author><author><name sortKey="Donna, Mb" uniqKey="Donna M">MB Donna</name></author><author><name sortKey="Bruno, As" uniqKey="Bruno A">AS Bruno</name></author><author><name sortKey="David, Cb" uniqKey="David C">CB David</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Qh" uniqKey="Zhu Q">QH Zhu</name></author><author><name sortKey="Fan, L" uniqKey="Fan L">L Fan</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Xu, H" uniqKey="Xu H">H Xu</name></author><author><name sortKey="Llewellyn, D" uniqKey="Llewellyn D">D Llewellyn</name></author><author><name sortKey="Wilson, I" uniqKey="Wilson I">I Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glazebrook, J" uniqKey="Glazebrook J">J Glazebrook</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Robert Seilaniantz, A" uniqKey="Robert Seilaniantz A">A Robert-Seilaniantz</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author><author><name sortKey="Bari, R" uniqKey="Bari R">R Bari</name></author><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grant, Mr" uniqKey="Grant M">MR Grant</name></author><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhaorong, M" uniqKey="Zhaorong M">M Zhaorong</name></author><author><name sortKey="Ceyda, C" uniqKey="Ceyda C">C Ceyda</name></author><author><name sortKey="Michael, Ja" uniqKey="Michael J">JA Michael</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Addo Quaye, C" uniqKey="Addo Quaye C">C Addo-Quaye</name></author><author><name sortKey="Eshoo, Tw" uniqKey="Eshoo T">TW Eshoo</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Addo Quaye, C" uniqKey="Addo Quaye C">C Addo-Quaye</name></author><author><name sortKey="Miller, W" uniqKey="Miller W">W Miller</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, S" uniqKey="Lu S">S Lu</name></author><author><name sortKey="Sun, Yh" uniqKey="Sun Y">YH Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peng, S" uniqKey="Peng S">S Peng</name></author><author><name sortKey="Dan, L" uniqKey="Dan L">L Dan</name></author><author><name sortKey="Zhoujia, Z" uniqKey="Zhoujia Z">Z Zhoujia</name></author><author><name sortKey="Weilun, Y" uniqKey="Weilun Y">Y Weilun</name></author><author><name sortKey="Xinli, X" uniqKey="Xinli X">X Xinli</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Genomics</journal-id><journal-id journal-id-type="iso-abbrev">BMC Genomics</journal-id><journal-title-group><journal-title>BMC Genomics</journal-title></journal-title-group><issn pub-type="epub">1471-2164</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26768277</article-id><article-id pub-id-type="pmc">4714501</article-id><article-id pub-id-type="publisher-id">2286</article-id><article-id pub-id-type="doi">10.1186/s12864-015-2286-6</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent <italic>Melampsora larici-populina</italic></article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Li</surname><given-names>Danlei</given-names></name><address><phone>+86-451-82190384</phone><email>danleili@126.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes" equal-contrib="yes"><name><surname>Wang</surname><given-names>Feng</given-names></name><address><phone>+86-451-82190384</phone><email>kingsummit@126.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Chao</given-names></name><address><phone>+86-451-82190384</phone><email>plantdisease@126.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Zou</surname><given-names>Li</given-names></name><address><phone>+86-451-82190384</phone><email>rustfungi@163.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Wang</surname><given-names>Zhiying</given-names></name><address><phone>+86-451-82191512</phone><email>nematodefungi@126.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Qiaoli</given-names></name><address><phone>+86-451-82190384</phone><email>melampsora@126.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Niu</surname><given-names>Chunyang</given-names></name><address><phone>+86-451-82190384</phone><email>virulencerust@126.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Ruizhi</given-names></name><address><phone>+86-451-82190384</phone><email>forestrydisease@163.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Ling</surname><given-names>Yaming</given-names></name><address><phone>+86-451-82190384</phone><email>kingrust@sina.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Bowen</given-names></name><address><phone>+86-451-82190384</phone><email>nematode2015@sina.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1">College of Forestry, Northeast Forestry University, Harbin, 150040 China</aff></contrib-group><pub-date pub-type="epub"><day>15</day><month>1</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>15</day><month>1</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>17</volume><elocation-id>59</elocation-id><history><date date-type="received"><day>26</day><month>8</month><year>2015</year></date><date date-type="accepted"><day>10</day><month>12</month><year>2015</year></date></history><permissions><copyright-statement>© Li et al. 2016</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>Rust caused by <italic>Melampsora larici-populina</italic> is one of the most damaging diseases of poplars. Rust is considered to be a model pathogen for genetic studies because both pathogen and host genomes are available. The poplar ‘Robusta’, whose general rust resistance is defeated by virulent rust E4, provides suitable host material for studies of the gene regulation involved in rust resistance/susceptibility. In this study, we investigated the microRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent rust. We were particularly interested in delineating the host-pathogen interactions with a specific focus on microRNAs (miRNAs).</p></sec><sec><title>Results</title><p>To study the susceptibility of poplar to <italic>M. larici-populina</italic>, small RNA (sRNA) libraries, a degradome cDNA library and digital gene expression libraries were constructed for rust-inoculated and rust-free susceptible poplar ‘Robusta’ leaves through high-throughput sequencing. Altogether, 12,722 regulating interactions were identified. The results delineated the framework of post-transcriptional regulation of gene expression in the susceptible poplar, which was infected by the virulent rust. The results indicated that pathogen-associated molecular patterns (PAMPs) and PAMP-triggered immunity were induced by the infection of virulent rust E4 and that miRNAs still functioned at this stage. After this stage, miRNA-regulated <italic>R</italic> genes, such as TIR-NBS-LRR and CC-NBS-LRR, were not fully functional. Additionally, the rust-responsive miRNAs did not regulate the signaling component genes related to the salicylic acid pathway or the hypersensitive response.</p></sec><sec><title>Conclusions</title><p>We found that the defense-related post-transcriptional regulation of the susceptible poplar ‘Robusta’ functions normally only at the stage of PAMPs and PAMP-triggered immunity (PTI). More importantly, the miRNA-mediated post-transcriptional regulation of defense signal pathway genes were inactivated by the infection of virulent rust at the stage of effector-triggered susceptibility and during the following stages of salicylic acid and hypersensitive responses. This inactivation was the major characteristic of ‘Robusta’ susceptibility.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12864-015-2286-6) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd><italic>Melampsora larici-populina</italic></kwd><kwd><italic>Populus</italic></kwd><kwd>Susceptibility</kwd><kwd>microRNAs</kwd><kwd>Degradome</kwd><kwd>DGE</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100001809</institution-id><institution>National Natural Science Foundation of China</institution></institution-wrap></funding-source><award-id>31500524</award-id><award-id>31300571</award-id><principal-award-recipient><name><surname>Li</surname><given-names>Danlei</given-names></name><name><surname>Wang</surname><given-names>Chao</given-names></name></principal-award-recipient></award-group><award-group><funding-source><institution>Fundamental Research Funds for the Central Universities</institution></funding-source><award-id>2572015CA09</award-id><award-id>2572015EY02</award-id><principal-award-recipient><name><surname>Wang</surname><given-names>Feng</given-names></name></principal-award-recipient></award-group><award-group><funding-source><institution>National High Technology Research and Development Program of China (863 Program)</institution></funding-source></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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