Small RNAs from the wheat stripe rust fungus (Puccinia striiformis f.sp. tritici)
Identifieur interne : 000140 ( Pmc/Curation ); précédent : 000139; suivant : 000141Small RNAs from the wheat stripe rust fungus (Puccinia striiformis f.sp. tritici)
Auteurs : Nicholas A. Mueth [États-Unis] ; Sowmya R. Ramachandran [États-Unis] ; Scot H. Hulbert [États-Unis]Source :
- BMC Genomics [ 1471-2164 ] ; 2015.
Abstract
Wheat stripe rust, caused by
We extracted small RNA from rust-infected wheat flag leaves and performed high-throughput sequencing. Two wheat cultivars were analyzed: one is susceptible; the other displays partial high-temperature adult plant resistance. Fungal-specific reads were identified by mapping to the
This work provides an inventory of small RNAs endogenous to an important plant pathogen, enabling further exploration of gene regulation on both sides of the host/parasite interaction. We conclude that small RNAs are likely to play a role in regulating the complex developmental processes involved in stripe rust pathogenicity.
The online version of this article (doi:10.1186/s12864-015-1895-4) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12864-015-1895-4
PubMed: 26391470
PubMed Central: 4578785
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Small RNAs from the wheat stripe rust fungus (<italic>Puccinia striiformis</italic> f.sp. <italic>tritici</italic>)</title><author><name sortKey="Mueth, Nicholas A" sort="Mueth, Nicholas A" uniqKey="Mueth N" first="Nicholas A." last="Mueth">Nicholas A. Mueth</name><affiliation wicri:level="2"><nlm:aff id="Aff1">Molecular Plant Sciences, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Molecular Plant Sciences, Washington State University, Pullman</wicri:cityArea></affiliation></author><author><name sortKey="Ramachandran, Sowmya R" sort="Ramachandran, Sowmya R" uniqKey="Ramachandran S" first="Sowmya R." last="Ramachandran">Sowmya R. Ramachandran</name><affiliation wicri:level="2"><nlm:aff id="Aff2">Plant Pathology, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Plant Pathology, Washington State University, Pullman</wicri:cityArea></affiliation></author><author><name sortKey="Hulbert, Scot H" sort="Hulbert, Scot H" uniqKey="Hulbert S" first="Scot H." last="Hulbert">Scot H. Hulbert</name><affiliation wicri:level="2"><nlm:aff id="Aff1">Molecular Plant Sciences, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Molecular Plant Sciences, Washington State University, Pullman</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff2">Plant Pathology, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Plant Pathology, Washington State University, Pullman</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26391470</idno><idno type="pmc">4578785</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578785</idno><idno type="RBID">PMC:4578785</idno><idno type="doi">10.1186/s12864-015-1895-4</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000140</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000140</idno><idno type="wicri:Area/Pmc/Curation">000140</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000140</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Small RNAs from the wheat stripe rust fungus (<italic>Puccinia striiformis</italic> f.sp. <italic>tritici</italic>)</title><author><name sortKey="Mueth, Nicholas A" sort="Mueth, Nicholas A" uniqKey="Mueth N" first="Nicholas A." last="Mueth">Nicholas A. Mueth</name><affiliation wicri:level="2"><nlm:aff id="Aff1">Molecular Plant Sciences, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Molecular Plant Sciences, Washington State University, Pullman</wicri:cityArea></affiliation></author><author><name sortKey="Ramachandran, Sowmya R" sort="Ramachandran, Sowmya R" uniqKey="Ramachandran S" first="Sowmya R." last="Ramachandran">Sowmya R. Ramachandran</name><affiliation wicri:level="2"><nlm:aff id="Aff2">Plant Pathology, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Plant Pathology, Washington State University, Pullman</wicri:cityArea></affiliation></author><author><name sortKey="Hulbert, Scot H" sort="Hulbert, Scot H" uniqKey="Hulbert S" first="Scot H." last="Hulbert">Scot H. Hulbert</name><affiliation wicri:level="2"><nlm:aff id="Aff1">Molecular Plant Sciences, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Molecular Plant Sciences, Washington State University, Pullman</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff2">Plant Pathology, Washington State University, Pullman, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Plant Pathology, Washington State University, Pullman</wicri:cityArea></affiliation></author></analytic><series><title level="j">BMC Genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Wheat stripe rust, caused by <italic>Puccinia striiformis</italic> f. sp. <italic>tritici</italic>, is a costly global disease that burdens farmers with yield loss and high fungicide expenses. This sophisticated biotrophic parasite infiltrates wheat leaves and develops infection structures inside host cells, appropriating nutrients while suppressing the plant defense response. Development in most eukaryotes is regulated by small RNA molecules, and the success of host-induced gene silencing technology in <italic>Puccinia spp</italic>. implies the existence of a functional RNAi system. However, some fungi lack this capability, and small RNAs have not yet been reported in rust fungi. The objective of this study was to determine whether <italic>P. striiformis</italic> carries an endogenous small RNA repertoire.</p></sec><sec><title>Results</title><p>We extracted small RNA from rust-infected wheat flag leaves and performed high-throughput sequencing. Two wheat cultivars were analyzed: one is susceptible; the other displays partial high-temperature adult plant resistance. Fungal-specific reads were identified by mapping to the <italic>P. striiformis</italic> draft genome and removing reads present in uninfected control libraries. Sequencing and bioinformatics results were verified by RT-PCR. Like other RNAi-equipped fungi, <italic>P. striiformis</italic> produces large numbers of 20–22 nt sequences with a preference for uracil at the 5′ position. Precise post-transcriptional processing and high accumulation of specific sRNA sequences were observed. Some predicted sRNA precursors possess a microRNA-like stem-loop secondary structure; others originate from much longer inverted repeats containing gene sequences. Finally, sRNA-target prediction algorithms were used to obtain a list of putative gene targets in both organisms. Predicted fungal target genes were enriched for kinases and small secreted proteins, while the list of wheat targets included homologs of known plant resistance genes.</p></sec><sec><title>Conclusions</title><p>This work provides an inventory of small RNAs endogenous to an important plant pathogen, enabling further exploration of gene regulation on both sides of the host/parasite interaction. We conclude that small RNAs are likely to play a role in regulating the complex developmental processes involved in stripe rust pathogenicity.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12864-015-1895-4) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Wellings, Cr" uniqKey="Wellings C">CR Wellings</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Line, Rf" uniqKey="Line R">RF Line</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brown, Jkm" uniqKey="Brown J">JKM Brown</name></author><author><name sortKey="Hovm Ller, Ms" uniqKey="Hovm Ller M">MS Hovmøller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hovm Ller, M" uniqKey="Hovm Ller M">M Hovmøller</name></author><author><name sortKey="Walter, S" uniqKey="Walter S">S Walter</name></author><author><name sortKey="Justesen, A" uniqKey="Justesen A">A Justesen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name></author><author><name sortKey="Wellings, C" uniqKey="Wellings C">C Wellings</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Kang, Z" uniqKey="Kang Z">Z Kang</name></author><author><name sortKey="Liu, T" uniqKey="Liu T">T Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yi, M" uniqKey="Yi M">M Yi</name></author><author><name sortKey="Valent, B" uniqKey="Valent B">B Valent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stergiopoulos, I" uniqKey="Stergiopoulos I">I Stergiopoulos</name></author><author><name sortKey="De Wit, Pjgm" uniqKey="De Wit P">PJGM de Wit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ellis, Jg" uniqKey="Ellis J">JG Ellis</name></author><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author><author><name sortKey="Lawrence, Gj" uniqKey="Lawrence G">GJ Lawrence</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cantu, D" uniqKey="Cantu D">D Cantu</name></author><author><name sortKey="Govindarajulu, M" uniqKey="Govindarajulu M">M Govindarajulu</name></author><author><name sortKey="Kozik, A" uniqKey="Kozik A">A Kozik</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Kojima, Kk" uniqKey="Kojima K">KK Kojima</name></author><author><name sortKey="Jurka, J" uniqKey="Jurka J">J Jurka</name></author><author><name sortKey="Michelmore, Rw" uniqKey="Michelmore R">RW Michelmore</name></author><author><name sortKey="Dubcovsky, J" uniqKey="Dubcovsky J">J Dubcovsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cantu, D" uniqKey="Cantu D">D Cantu</name></author><author><name sortKey="Segovia, V" uniqKey="Segovia V">V Segovia</name></author><author><name sortKey="Maclean, D" uniqKey="Maclean D">D Maclean</name></author><author><name sortKey="Bayles, R" uniqKey="Bayles R">R Bayles</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Kamoun, S" uniqKey="Kamoun S">S Kamoun</name></author><author><name sortKey="Dubcovsky, J" uniqKey="Dubcovsky J">J Dubcovsky</name></author><author><name sortKey="Saunders, Dg" uniqKey="Saunders D">DG Saunders</name></author><author><name sortKey="Uauy, C" uniqKey="Uauy C">C Uauy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Westermann, Aj" uniqKey="Westermann A">AJ Westermann</name></author><author><name sortKey="Gorski, Sa" uniqKey="Gorski S">SA Gorski</name></author><author><name sortKey="Vogel, J" uniqKey="Vogel J">J Vogel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Asai, S" uniqKey="Asai S">S Asai</name></author><author><name sortKey="Rallapalli, G" uniqKey="Rallapalli G">G Rallapalli</name></author><author><name sortKey="Piquerez, Sjm" uniqKey="Piquerez S">SJM Piquerez</name></author><author><name sortKey="Caillaud, M C" uniqKey="Caillaud M">M-C Caillaud</name></author><author><name sortKey="Furzer, Oj" uniqKey="Furzer O">OJ Furzer</name></author><author><name sortKey="Ishaque, N" uniqKey="Ishaque N">N Ishaque</name></author><author><name sortKey="Wirthmueller, L" uniqKey="Wirthmueller L">L Wirthmueller</name></author><author><name sortKey="Fabro, G" uniqKey="Fabro G">G Fabro</name></author><author><name sortKey="Shirasu, K" uniqKey="Shirasu K">K Shirasu</name></author><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poethig, Rs" uniqKey="Poethig R">RS Poethig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knauer, S" uniqKey="Knauer S">S Knauer</name></author><author><name sortKey="Holt, Al" uniqKey="Holt A">AL Holt</name></author><author><name sortKey="Rubio Somoza, I" uniqKey="Rubio Somoza I">I Rubio-Somoza</name></author><author><name sortKey="Tucker, Ej" uniqKey="Tucker E">EJ Tucker</name></author><author><name sortKey="Hinze, A" uniqKey="Hinze A">A Hinze</name></author><author><name sortKey="Pisch, M" uniqKey="Pisch M">M Pisch</name></author><author><name sortKey="Javelle, M" uniqKey="Javelle M">M Javelle</name></author><author><name sortKey="Timmermans, Mc" uniqKey="Timmermans M">MC Timmermans</name></author><author><name sortKey="Tucker, Mr" uniqKey="Tucker M">MR Tucker</name></author><author><name sortKey="Laux, T" uniqKey="Laux T">T Laux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Katiyar Agarwal, S" uniqKey="Katiyar Agarwal S">S Katiyar-Agarwal</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author><author><name sortKey="Dunoyer, P" uniqKey="Dunoyer P">P Dunoyer</name></author><author><name sortKey="Jay, F" uniqKey="Jay F">F Jay</name></author><author><name sortKey="Arnold, B" uniqKey="Arnold B">B Arnold</name></author><author><name sortKey="Dharmasiri, N" uniqKey="Dharmasiri N">N Dharmasiri</name></author><author><name sortKey="Estelle, M" uniqKey="Estelle M">M Estelle</name></author><author><name sortKey="Voinnet, O" uniqKey="Voinnet O">O Voinnet</name></author><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Lu, Y G" uniqKey="Lu Y">Y-G Lu</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y Shi</name></author><author><name sortKey="Wu, L" uniqKey="Wu L">L Wu</name></author><author><name sortKey="Xu, Y J" uniqKey="Xu Y">Y-J Xu</name></author><author><name sortKey="Huang, F" uniqKey="Huang F">F Huang</name></author><author><name sortKey="Guo, X Y" uniqKey="Guo X">X-Y Guo</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Fan, J" uniqKey="Fan J">J Fan</name></author><author><name sortKey="Zhao, J Q" uniqKey="Zhao J">J-Q Zhao</name></author><author><name sortKey="Zhang, H Y" uniqKey="Zhang H">H-Y Zhang</name></author><author><name sortKey="Xu, P Z" uniqKey="Xu P">P-Z Xu</name></author><author><name sortKey="Zhou, J M" uniqKey="Zhou J">J-M Zhou</name></author><author><name sortKey="Wu, X J" uniqKey="Wu X">X-J Wu</name></author><author><name sortKey="Wang, P R" uniqKey="Wang P">P-R Wang</name></author><author><name sortKey="Wang, W M" uniqKey="Wang W">W-M Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romano, N" uniqKey="Romano N">N Romano</name></author><author><name sortKey="Macino, G" uniqKey="Macino G">G Macino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nunes, Cc" uniqKey="Nunes C">CC Nunes</name></author><author><name sortKey="Sailsbery, Jk" uniqKey="Sailsbery J">JK Sailsbery</name></author><author><name sortKey="Dean, Ra" uniqKey="Dean R">RA Dean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drinnenberg, Ia" uniqKey="Drinnenberg I">IA Drinnenberg</name></author><author><name sortKey="Fink, Gr" uniqKey="Fink G">GR Fink</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drinnenberg, Ia" uniqKey="Drinnenberg I">IA Drinnenberg</name></author><author><name sortKey="Weinberg, De" uniqKey="Weinberg D">DE Weinberg</name></author><author><name sortKey="Xie, Kt" uniqKey="Xie K">KT Xie</name></author><author><name sortKey="Mower, Jp" uniqKey="Mower J">JP Mower</name></author><author><name sortKey="Wolfe, Kh" uniqKey="Wolfe K">KH Wolfe</name></author><author><name sortKey="Fink, Gr" uniqKey="Fink G">GR Fink</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fahlgren, N" uniqKey="Fahlgren N">N Fahlgren</name></author><author><name sortKey="Bollmann, Sr" uniqKey="Bollmann S">SR Bollmann</name></author><author><name sortKey="Kasschau, Kd" uniqKey="Kasschau K">KD Kasschau</name></author><author><name sortKey="Cuperus, Jt" uniqKey="Cuperus J">JT Cuperus</name></author><author><name sortKey="Press, Cm" uniqKey="Press C">CM Press</name></author><author><name sortKey="Sullivan, Cm" uniqKey="Sullivan C">CM Sullivan</name></author><author><name sortKey="Chapman, Ej" uniqKey="Chapman E">EJ Chapman</name></author><author><name sortKey="Hoyer, Js" uniqKey="Hoyer J">JS Hoyer</name></author><author><name sortKey="Gilbert, Kb" uniqKey="Gilbert K">KB Gilbert</name></author><author><name sortKey="Grunwald, Nj" uniqKey="Grunwald N">NJ Grünwald</name></author><author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, N" uniqKey="Jiang N">N Jiang</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name></author><author><name sortKey="Janbon, G" uniqKey="Janbon G">G Janbon</name></author><author><name sortKey="Pan, J" uniqKey="Pan J">J Pan</name></author><author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nunes, Cc" uniqKey="Nunes C">CC Nunes</name></author><author><name sortKey="Gowda, M" uniqKey="Gowda M">M Gowda</name></author><author><name sortKey="Sailsbery, J" uniqKey="Sailsbery J">J Sailsbery</name></author><author><name sortKey="Xue, M" uniqKey="Xue M">M Xue</name></author><author><name sortKey="Chen, F" uniqKey="Chen F">F Chen</name></author><author><name sortKey="Brown, De" uniqKey="Brown D">DE Brown</name></author><author><name sortKey="Oh, Y" uniqKey="Oh Y">Y Oh</name></author><author><name sortKey="Mitchell, Tk" uniqKey="Mitchell T">TK Mitchell</name></author><author><name sortKey="Dean, Ra" uniqKey="Dean R">RA Dean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiberg, A" uniqKey="Weiberg A">A Weiberg</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Lin, F M" uniqKey="Lin F">F-M Lin</name></author><author><name sortKey="Zhao, H" uniqKey="Zhao H">H Zhao</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Kaloshian, I" uniqKey="Kaloshian I">I Kaloshian</name></author><author><name sortKey="Huang, H D" uniqKey="Huang H">H-D Huang</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiberg, A" uniqKey="Weiberg A">A Weiberg</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Bellinger, M" uniqKey="Bellinger M">M Bellinger</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baulcombe, D" uniqKey="Baulcombe D">D Baulcombe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nunes, Cc" uniqKey="Nunes C">CC Nunes</name></author><author><name sortKey="Dean, Ra" uniqKey="Dean R">RA Dean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nowara, D" uniqKey="Nowara D">D Nowara</name></author><author><name sortKey="Gay, A" uniqKey="Gay A">A Gay</name></author><author><name sortKey="Lacomme, C" uniqKey="Lacomme C">C Lacomme</name></author><author><name sortKey="Shaw, J" uniqKey="Shaw J">J Shaw</name></author><author><name sortKey="Ridout, C" uniqKey="Ridout C">C Ridout</name></author><author><name sortKey="Douchkov, D" uniqKey="Douchkov D">D Douchkov</name></author><author><name sortKey="Hensel, G" uniqKey="Hensel G">G Hensel</name></author><author><name sortKey="Kumlehn, J" uniqKey="Kumlehn J">J Kumlehn</name></author><author><name sortKey="Schweizer, P" uniqKey="Schweizer P">P Schweizer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, C" uniqKey="Yin C">C Yin</name></author><author><name sortKey="Jurgenson, Je" uniqKey="Jurgenson J">JE Jurgenson</name></author><author><name sortKey="Hulbert, Sh" uniqKey="Hulbert S">SH Hulbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Panwar, V" uniqKey="Panwar V">V Panwar</name></author><author><name sortKey="Mccallum, B" uniqKey="Mccallum B">B McCallum</name></author><author><name sortKey="Bakkeren, G" uniqKey="Bakkeren G">G Bakkeren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, C" uniqKey="Yin C">C Yin</name></author><author><name sortKey="Park, J J" uniqKey="Park J">J-J Park</name></author><author><name sortKey="Gang, Dr" uniqKey="Gang D">DR Gang</name></author><author><name sortKey="Hulbert, Sh" uniqKey="Hulbert S">SH Hulbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, H C" uniqKey="Lee H">H-C Lee</name></author><author><name sortKey="Chang, S S" uniqKey="Chang S">S-S Chang</name></author><author><name sortKey="Choudhary, S" uniqKey="Choudhary S">S Choudhary</name></author><author><name sortKey="Aalto, Ap" uniqKey="Aalto A">AP Aalto</name></author><author><name sortKey="Maiti, M" uniqKey="Maiti M">M Maiti</name></author><author><name sortKey="Bamford, Dh" uniqKey="Bamford D">DH Bamford</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, C" uniqKey="Yin C">C Yin</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Han, Q" uniqKey="Han Q">Q Han</name></author><author><name sortKey="Kang, Z" uniqKey="Kang Z">Z Kang</name></author><author><name sortKey="Hulbert, Sh" uniqKey="Hulbert S">SH Hulbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carter, Ah" uniqKey="Carter A">AH Carter</name></author><author><name sortKey="Chen, Xm" uniqKey="Chen X">XM Chen</name></author><author><name sortKey="Garland Campbell, K" uniqKey="Garland Campbell K">K Garland-Campbell</name></author><author><name sortKey="Kidwell, Kk" uniqKey="Kidwell K">KK Kidwell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="S Rensen, Ck" uniqKey="S Rensen C">CK Sørensen</name></author><author><name sortKey="Justesen, Af" uniqKey="Justesen A">AF Justesen</name></author><author><name sortKey="Hovm Ller, Ms" uniqKey="Hovm Ller M">MS Hovmøller</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kozomara, A" uniqKey="Kozomara A">A Kozomara</name></author><author><name sortKey="Griffiths Jones, S" uniqKey="Griffiths Jones S">S Griffiths-Jones</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ro, S" uniqKey="Ro S">S Ro</name></author><author><name sortKey="Yan, W" uniqKey="Yan W">W Yan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, H C" uniqKey="Lee H">H-C Lee</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Gu, W" uniqKey="Gu W">W Gu</name></author><author><name sortKey="Xue, Z" uniqKey="Xue Z">Z Xue</name></author><author><name sortKey="Crosthwaite, Sk" uniqKey="Crosthwaite S">SK Crosthwaite</name></author><author><name sortKey="Pertsemlidis, A" uniqKey="Pertsemlidis A">A Pertsemlidis</name></author><author><name sortKey="Lewis, Za" uniqKey="Lewis Z">ZA Lewis</name></author><author><name sortKey="Freitag, M" uniqKey="Freitag M">M Freitag</name></author><author><name sortKey="Selker, Eu" uniqKey="Selker E">EU Selker</name></author><author><name sortKey="Mello, Cc" uniqKey="Mello C">CC Mello</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Skp" uniqKey="Lau S">SKP Lau</name></author><author><name sortKey="Chow, W N" uniqKey="Chow W">W-N Chow</name></author><author><name sortKey="Wong, Ayp" uniqKey="Wong A">AYP Wong</name></author><author><name sortKey="Yeung, Jmy" uniqKey="Yeung J">JMY Yeung</name></author><author><name sortKey="Bao, J" uniqKey="Bao J">J Bao</name></author><author><name sortKey="Zhang, N" uniqKey="Zhang N">N Zhang</name></author><author><name sortKey="Lok, S" uniqKey="Lok S">S Lok</name></author><author><name sortKey="Woo, Pcy" uniqKey="Woo P">PCY Woo</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K-Y Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyers, Bc" uniqKey="Meyers B">BC Meyers</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author><author><name sortKey="Bartel, B" uniqKey="Bartel B">B Bartel</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author><author><name sortKey="Baulcombe, D" uniqKey="Baulcombe D">D Baulcombe</name></author><author><name sortKey="Bowman, Jl" uniqKey="Bowman J">JL Bowman</name></author><author><name sortKey="Cao, X" uniqKey="Cao X">X Cao</name></author><author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Green, Pj" uniqKey="Green P">PJ Green</name></author><author><name sortKey="Griffiths Jones, S" uniqKey="Griffiths Jones S">S Griffiths-Jones</name></author><author><name sortKey="Jacobsen, Se" uniqKey="Jacobsen S">SE Jacobsen</name></author><author><name sortKey="Mallory, Ac" uniqKey="Mallory A">AC Mallory</name></author><author><name sortKey="Martienssen, Ra" uniqKey="Martienssen R">RA Martienssen</name></author><author><name sortKey="Poethig, Rs" uniqKey="Poethig R">RS Poethig</name></author><author><name sortKey="Qi, Y" uniqKey="Qi Y">Y Qi</name></author><author><name sortKey="Vaucheret, H" uniqKey="Vaucheret H">H Vaucheret</name></author><author><name sortKey="Voinnet, O" uniqKey="Voinnet O">O Voinnet</name></author><author><name sortKey="Watanabe, Y" uniqKey="Watanabe Y">Y Watanabe</name></author><author><name sortKey="Weigel, D" uniqKey="Weigel D">D Weigel</name></author><author><name sortKey="Zhu, J K" uniqKey="Zhu J">J-K Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Gao, S" uniqKey="Gao S">S Gao</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X Zhou</name></author><author><name sortKey="Xia, J" uniqKey="Xia J">J Xia</name></author><author><name sortKey="Chellappan, P" uniqKey="Chellappan P">P Chellappan</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X Zhou</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author><author><name sortKey="Westholm, Jo" uniqKey="Westholm J">JO Westholm</name></author><author><name sortKey="Lai, Ec" uniqKey="Lai E">EC Lai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lowe, Tm" uniqKey="Lowe T">TM Lowe</name></author><author><name sortKey="Eddy, Sr" uniqKey="Eddy S">SR Eddy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Donaldson, Me" uniqKey="Donaldson M">ME Donaldson</name></author><author><name sortKey="Saville, Bj" uniqKey="Saville B">BJ Saville</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dai, X" uniqKey="Dai X">X Dai</name></author><author><name sortKey="Zhao, Px" uniqKey="Zhao P">PX Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonnet, E" uniqKey="Bonnet E">E Bonnet</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Billiau, K" uniqKey="Billiau K">K Billiau</name></author><author><name sortKey="Van De Peer, Y" uniqKey="Van De Peer Y">Y Van de Peer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fahlgren, N" uniqKey="Fahlgren N">N Fahlgren</name></author><author><name sortKey="Howell, Md" uniqKey="Howell M">MD Howell</name></author><author><name sortKey="Kasschau, Kd" uniqKey="Kasschau K">KD Kasschau</name></author><author><name sortKey="Chapman, Ej" uniqKey="Chapman E">EJ Chapman</name></author><author><name sortKey="Sullivan, Cm" uniqKey="Sullivan C">CM Sullivan</name></author><author><name sortKey="Cumbie, Js" uniqKey="Cumbie J">JS Cumbie</name></author><author><name sortKey="Givan, Sa" uniqKey="Givan S">SA Givan</name></author><author><name sortKey="Law, Tf" uniqKey="Law T">TF Law</name></author><author><name sortKey="Grant, Sr" uniqKey="Grant S">SR Grant</name></author><author><name sortKey="Dangl, Jl" uniqKey="Dangl J">JL Dangl</name></author><author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Srivastava, Pk" uniqKey="Srivastava P">PK Srivastava</name></author><author><name sortKey="Moturu, T" uniqKey="Moturu T">T Moturu</name></author><author><name sortKey="Pandey, P" uniqKey="Pandey P">P Pandey</name></author><author><name sortKey="Baldwin, It" uniqKey="Baldwin I">IT Baldwin</name></author><author><name sortKey="Pandey, Sp" uniqKey="Pandey S">SP Pandey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saunders, Dgo" uniqKey="Saunders D">DGO Saunders</name></author><author><name sortKey="Win, J" uniqKey="Win J">J Win</name></author><author><name sortKey="Cano, Lm" uniqKey="Cano L">LM Cano</name></author><author><name sortKey="Szabo, Lj" uniqKey="Szabo L">LJ Szabo</name></author><author><name sortKey="Kamoun, S" uniqKey="Kamoun S">S Kamoun</name></author><author><name sortKey="Raffaele, S" uniqKey="Raffaele S">S Raffaele</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petersen, Tn" uniqKey="Petersen T">TN Petersen</name></author><author><name sortKey="Brunak, S" uniqKey="Brunak S">S Brunak</name></author><author><name sortKey="Von Heijne, G" uniqKey="Von Heijne G">G von Heijne</name></author><author><name sortKey="Nielsen, H" uniqKey="Nielsen H">H Nielsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Godfrey, D" uniqKey="Godfrey D">D Godfrey</name></author><author><name sortKey="Bohlenius, H" uniqKey="Bohlenius H">H Böhlenius</name></author><author><name sortKey="Pedersen, C" uniqKey="Pedersen C">C Pedersen</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Emmersen, J" uniqKey="Emmersen J">J Emmersen</name></author><author><name sortKey="Thordal Christensen, H" uniqKey="Thordal Christensen H">H Thordal-Christensen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hulbert, S" uniqKey="Hulbert S">S Hulbert</name></author><author><name sortKey="Pumphrey, M" uniqKey="Pumphrey M">M Pumphrey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Llave, C" uniqKey="Llave C">C Llave</name></author><author><name sortKey="Franco Zorrilla, Jm" uniqKey="Franco Zorrilla J">JM Franco-Zorrilla</name></author><author><name sortKey="Solano, R" uniqKey="Solano R">R Solano</name></author><author><name sortKey="Barajas, D" uniqKey="Barajas D">D Barajas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gijzen, M" uniqKey="Gijzen M">M Gijzen</name></author><author><name sortKey="Ishmael, C" uniqKey="Ishmael C">C Ishmael</name></author><author><name sortKey="Shrestha, Sd" uniqKey="Shrestha S">SD Shrestha</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="German, Ma" uniqKey="German M">MA German</name></author><author><name sortKey="Pillay, M" uniqKey="Pillay M">M Pillay</name></author><author><name sortKey="Jeong, D H" uniqKey="Jeong D">D-H Jeong</name></author><author><name sortKey="Hetawal, A" uniqKey="Hetawal A">A Hetawal</name></author><author><name sortKey="Luo, S" uniqKey="Luo S">S Luo</name></author><author><name sortKey="Janardhanan, P" uniqKey="Janardhanan P">P Janardhanan</name></author><author><name sortKey="Kannan, V" uniqKey="Kannan V">V Kannan</name></author><author><name sortKey="Rymarquis, La" uniqKey="Rymarquis L">LA Rymarquis</name></author><author><name sortKey="Nobuta, K" uniqKey="Nobuta K">K Nobuta</name></author><author><name sortKey="German, R" uniqKey="German R">R German</name></author><author><name sortKey="De Paoli, E" uniqKey="De Paoli E">E De Paoli</name></author><author><name sortKey="Lu, C" uniqKey="Lu C">C Lu</name></author><author><name sortKey="Schroth, G" uniqKey="Schroth G">G Schroth</name></author><author><name sortKey="Meyers, Bc" uniqKey="Meyers B">BC Meyers</name></author><author><name sortKey="Green, Pj" uniqKey="Green P">PJ Green</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y F" uniqKey="Li Y">Y-F Li</name></author><author><name sortKey="Zheng, Y" uniqKey="Zheng Y">Y Zheng</name></author><author><name sortKey="Jagadeeswaran, G" uniqKey="Jagadeeswaran G">G Jagadeeswaran</name></author><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moldenhauer, J" uniqKey="Moldenhauer J">J Moldenhauer</name></author><author><name sortKey="Moerschbacher, Bm" uniqKey="Moerschbacher B">BM Moerschbacher</name></author><author><name sortKey="Van Der Westhuizen, Aj" uniqKey="Van Der Westhuizen A">AJ van der Westhuizen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Robinson, Md" uniqKey="Robinson M">MD Robinson</name></author><author><name sortKey="Mccarthy, Dj" uniqKey="Mccarthy D">DJ McCarthy</name></author><author><name sortKey="Smyth, Gk" uniqKey="Smyth G">GK Smyth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hulsen, T" uniqKey="Hulsen T">T Hulsen</name></author><author><name sortKey="De Vlieg, J" uniqKey="De Vlieg J">J de Vlieg</name></author><author><name sortKey="Alkema, W" uniqKey="Alkema W">W Alkema</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Genomics</journal-id><journal-id journal-id-type="iso-abbrev">BMC Genomics</journal-id><journal-title-group><journal-title>BMC Genomics</journal-title></journal-title-group><issn pub-type="epub">1471-2164</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26391470</article-id><article-id pub-id-type="pmc">4578785</article-id><article-id pub-id-type="publisher-id">1895</article-id><article-id pub-id-type="doi">10.1186/s12864-015-1895-4</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Small RNAs from the wheat stripe rust fungus (<italic>Puccinia striiformis</italic> f.sp. <italic>tritici</italic>)</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mueth</surname><given-names>Nicholas A.</given-names></name><address><email>nicholas.mueth@wsu.edu</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Ramachandran</surname><given-names>Sowmya R.</given-names></name><address><email>sowmya.ramachandran@wsu.edu</email></address><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Hulbert</surname><given-names>Scot H.</given-names></name><address><email>scot_hulbert@wsu.edu</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><aff id="Aff1"><label></label>Molecular Plant Sciences, Washington State University, Pullman, WA USA</aff><aff id="Aff2"><label></label>Plant Pathology, Washington State University, Pullman, WA USA</aff></contrib-group><pub-date pub-type="epub"><day>21</day><month>9</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>21</day><month>9</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>16</volume><issue>1</issue><elocation-id>718</elocation-id><history><date date-type="received"><day>5</day><month>6</month><year>2015</year></date><date date-type="accepted"><day>6</day><month>9</month><year>2015</year></date></history><permissions><copyright-statement>© Mueth et al. 2015</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>Wheat stripe rust, caused by <italic>Puccinia striiformis</italic> f. sp. <italic>tritici</italic>, is a costly global disease that burdens farmers with yield loss and high fungicide expenses. This sophisticated biotrophic parasite infiltrates wheat leaves and develops infection structures inside host cells, appropriating nutrients while suppressing the plant defense response. Development in most eukaryotes is regulated by small RNA molecules, and the success of host-induced gene silencing technology in <italic>Puccinia spp</italic>. implies the existence of a functional RNAi system. However, some fungi lack this capability, and small RNAs have not yet been reported in rust fungi. The objective of this study was to determine whether <italic>P. striiformis</italic> carries an endogenous small RNA repertoire.</p></sec><sec><title>Results</title><p>We extracted small RNA from rust-infected wheat flag leaves and performed high-throughput sequencing. Two wheat cultivars were analyzed: one is susceptible; the other displays partial high-temperature adult plant resistance. Fungal-specific reads were identified by mapping to the <italic>P. striiformis</italic> draft genome and removing reads present in uninfected control libraries. Sequencing and bioinformatics results were verified by RT-PCR. Like other RNAi-equipped fungi, <italic>P. striiformis</italic> produces large numbers of 20–22 nt sequences with a preference for uracil at the 5′ position. Precise post-transcriptional processing and high accumulation of specific sRNA sequences were observed. Some predicted sRNA precursors possess a microRNA-like stem-loop secondary structure; others originate from much longer inverted repeats containing gene sequences. Finally, sRNA-target prediction algorithms were used to obtain a list of putative gene targets in both organisms. Predicted fungal target genes were enriched for kinases and small secreted proteins, while the list of wheat targets included homologs of known plant resistance genes.</p></sec><sec><title>Conclusions</title><p>This work provides an inventory of small RNAs endogenous to an important plant pathogen, enabling further exploration of gene regulation on both sides of the host/parasite interaction. We conclude that small RNAs are likely to play a role in regulating the complex developmental processes involved in stripe rust pathogenicity.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12864-015-1895-4) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd><italic>Puccinia</italic></kwd><kwd>Pathogen</kwd><kwd>Fungus</kwd><kwd>Stripe rust</kwd><kwd>Wheat</kwd><kwd>Small RNA</kwd><kwd>miRNA</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2015</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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