Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi
Identifieur interne : 000F17 ( Main/Exploration ); précédent : 000F16; suivant : 000F18Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi
Auteurs : Aïda Azaiez [Canada] ; Brian Boyle [Canada] ; Valérie Levee [Canada] ; Armand Seguin [Canada]Source :
- Molecular plant-microbe interactions [ 0894-0282 ] ; 2009.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Anti-infectieux (métabolisme), Basidiomycota (physiologie), Chimère (métabolisme), Clones cellulaires (MeSH), Feuilles de plante (génétique), Feuilles de plante (microbiologie), Gènes de plante (MeSH), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Paroi cellulaire (génétique), Populus (génétique), Populus (microbiologie), Populus (métabolisme), Reproductibilité des résultats (MeSH), Réaction de polymérisation en chaîne (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Séquençage par oligonucléotides en batterie (MeSH), Transduction du signal (MeSH), Transporteurs ABC (métabolisme).
- MESH :
- génétique : Feuilles de plante, Maladies des plantes, Paroi cellulaire, Populus.
- microbiologie : Feuilles de plante, Maladies des plantes, Populus.
- métabolisme : Anti-infectieux, Chimère, Populus, Transporteurs ABC.
- physiologie : Basidiomycota.
- Pascal (Inist)
- Analyse de profil d'expression de gènes, Clones cellulaires, Gènes de plante, Reproductibilité des résultats, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes végétaux, Séquençage par oligonucléotides en batterie, Transcriptome, Melampsora, Analyse quantitative, Résistance, Microbiologie, Plante, Transduction du signal.
- Wicri :
- topic : Analyse quantitative.
English descriptors
- KwdEn :
- ATP-Binding Cassette Transporters (metabolism), Anti-Infective Agents (metabolism), Basidiomycota (physiology), Cell Wall (genetics), Chimera (metabolism), Clone Cells (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Melampsora, Microbiology, Oligonucleotide Array Sequence Analysis (MeSH), Plant, Plant Diseases (genetics), Plant Diseases (microbiology), Plant Leaves (genetics), Plant Leaves (microbiology), Polymerase Chain Reaction (MeSH), Populus (genetics), Populus (metabolism), Populus (microbiology), Quantitative analysis, Reproducibility of Results (MeSH), Resistance, Signal Transduction (MeSH), Transcriptome.
- MESH :
- chemical , metabolism : ATP-Binding Cassette Transporters, Anti-Infective Agents.
- genetics : Cell Wall, Plant Diseases, Plant Leaves, Populus.
- metabolism : Chimera, Populus.
- microbiology : Plant Diseases, Plant Leaves, Populus.
- physiology : Basidiomycota.
- Clone Cells, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Reproducibility of Results, Signal Transduction.
Abstract
In natural conditions, plants are subjected to a combination of biotic stresses and often have to cope with simultaneous pathogen infections. In this report, we aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra × P. maximowiczii) to infection by two biotrophic Melampsora fungi, Melampsora larici-populina and M. medusae f. sp. deltoidae. These pathogens triggered different responses after inoculation of poplar leaves. Transcript profiling using the GeneChip Poplar Genome Array revealed a total of 416 differentially expressed transcripts whose expression level was >twofold relative to controls. Interestingly, approximately half of the differentially expressed genes in infected leaves showed altered expression following interaction with either of the Melampsora spp. We also infected poplar leaves simultaneously with both Melampsora spp. to investigate potential interaction between the responses to the individual pathogens during a mixed infection. For this mixed inoculation, the number of differentially expressed transcripts increased to 648 and our analysis showed that infection with both fungi also induced a common set of genes. The genes induced after Melampsora spp. infection were mainly related to primary and secondary metabolic processes, cell-wall reinforcement and lignification, defense and stress-related mechanisms, and signal perception and transduction.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000028
- to stream PascalFrancis, to step Curation: 000102
- to stream PascalFrancis, to step Checkpoint: 000022
- to stream Main, to step Merge: 000F35
- to stream PubMed, to step Corpus: 000129
- to stream PubMed, to step Curation: 000129
- to stream PubMed, to step Checkpoint: 000123
- to stream Ncbi, to step Merge: 000155
- to stream Ncbi, to step Curation: 000155
- to stream Ncbi, to step Checkpoint: 000155
- to stream Main, to step Merge: 000E48
- to stream Main, to step Curation: 000F17
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi</title><author><name sortKey="Azaiez, Aida" sort="Azaiez, Aida" uniqKey="Azaiez A" first="Aïda" last="Azaiez">Aïda Azaiez</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Boyle, Brian" sort="Boyle, Brian" uniqKey="Boyle B" first="Brian" last="Boyle">Brian Boyle</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Levee, Valerie" sort="Levee, Valerie" uniqKey="Levee V" first="Valérie" last="Levee">Valérie Levee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Seguin">Armand Seguin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">09-0122066</idno><date when="2009">2009</date><idno type="stanalyst">PASCAL 09-0122066 INIST</idno><idno type="RBID">Pascal:09-0122066</idno><idno type="wicri:Area/PascalFrancis/Corpus">000028</idno><idno type="wicri:Area/PascalFrancis/Curation">000102</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000022</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000022</idno><idno type="wicri:doubleKey">0894-0282:2009:Azaiez A:transcriptome:profiling:in</idno><idno type="wicri:Area/Main/Merge">000F35</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:19132871</idno><idno type="wicri:Area/PubMed/Corpus">000129</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000129</idno><idno type="wicri:Area/PubMed/Curation">000129</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000129</idno><idno type="wicri:Area/PubMed/Checkpoint">000123</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000123</idno><idno type="wicri:Area/Ncbi/Merge">000155</idno><idno type="wicri:Area/Ncbi/Curation">000155</idno><idno type="wicri:Area/Ncbi/Checkpoint">000155</idno><idno type="wicri:doubleKey">0894-0282:2009:Azaiez A:transcriptome:profiling:in</idno><idno type="wicri:Area/Main/Merge">000E48</idno><idno type="wicri:Area/Main/Curation">000F17</idno><idno type="wicri:Area/Main/Exploration">000F17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi</title><author><name sortKey="Azaiez, Aida" sort="Azaiez, Aida" uniqKey="Azaiez A" first="Aïda" last="Azaiez">Aïda Azaiez</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Boyle, Brian" sort="Boyle, Brian" uniqKey="Boyle B" first="Brian" last="Boyle">Brian Boyle</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Levee, Valerie" sort="Levee, Valerie" uniqKey="Levee V" first="Valérie" last="Levee">Valérie Levee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Seguin">Armand Seguin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1><s2>Quebec, QC G1V 4C7</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Molecular plant-microbe interactions</title><title level="j" type="abbreviated">Mol. plant-microb. interact.</title><idno type="ISSN">0894-0282</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Molecular plant-microbe interactions</title><title level="j" type="abbreviated">Mol. plant-microb. interact.</title><idno type="ISSN">0894-0282</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ATP-Binding Cassette Transporters (metabolism)</term><term>Anti-Infective Agents (metabolism)</term><term>Basidiomycota (physiology)</term><term>Cell Wall (genetics)</term><term>Chimera (metabolism)</term><term>Clone Cells (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Melampsora</term><term>Microbiology</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Plant</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (microbiology)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Populus (microbiology)</term><term>Quantitative analysis</term><term>Reproducibility of Results (MeSH)</term><term>Resistance</term><term>Signal Transduction (MeSH)</term><term>Transcriptome</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Anti-infectieux (métabolisme)</term><term>Basidiomycota (physiologie)</term><term>Chimère (métabolisme)</term><term>Clones cellulaires (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (microbiologie)</term><term>Gènes de plante (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Paroi cellulaire (génétique)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Populus (métabolisme)</term><term>Reproductibilité des résultats (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquençage par oligonucléotides en batterie (MeSH)</term><term>Transduction du signal (MeSH)</term><term>Transporteurs ABC (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>ATP-Binding Cassette Transporters</term><term>Anti-Infective Agents</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Wall</term><term>Plant Diseases</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Paroi cellulaire</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chimera</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anti-infectieux</term><term>Chimère</term><term>Populus</term><term>Transporteurs ABC</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Clone Cells</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Oligonucleotide Array Sequence Analysis</term><term>Polymerase Chain Reaction</term><term>Reproducibility of Results</term><term>Signal Transduction</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Clones cellulaires</term><term>Gènes de plante</term><term>Reproductibilité des résultats</term><term>Réaction de polymérisation en chaîne</term><term>Régulation de l'expression des gènes végétaux</term><term>Séquençage par oligonucléotides en batterie</term><term>Transcriptome</term><term>Melampsora</term><term>Analyse quantitative</term><term>Résistance</term><term>Microbiologie</term><term>Plante</term><term>Transduction du signal</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Analyse quantitative</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In natural conditions, plants are subjected to a combination of biotic stresses and often have to cope with simultaneous pathogen infections. In this report, we aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra × P. maximowiczii) to infection by two biotrophic Melampsora fungi, Melampsora larici-populina and M. medusae f. sp. deltoidae. These pathogens triggered different responses after inoculation of poplar leaves. Transcript profiling using the GeneChip Poplar Genome Array revealed a total of 416 differentially expressed transcripts whose expression level was >twofold relative to controls. Interestingly, approximately half of the differentially expressed genes in infected leaves showed altered expression following interaction with either of the Melampsora spp. We also infected poplar leaves simultaneously with both Melampsora spp. to investigate potential interaction between the responses to the individual pathogens during a mixed infection. For this mixed inoculation, the number of differentially expressed transcripts increased to 648 and our analysis showed that infection with both fungi also induced a common set of genes. The genes induced after Melampsora spp. infection were mainly related to primary and secondary metabolic processes, cell-wall reinforcement and lignification, defense and stress-related mechanisms, and signal perception and transduction.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Azaiez, Aida" sort="Azaiez, Aida" uniqKey="Azaiez A" first="Aïda" last="Azaiez">Aïda Azaiez</name></noRegion><name sortKey="Boyle, Brian" sort="Boyle, Brian" uniqKey="Boyle B" first="Brian" last="Boyle">Brian Boyle</name><name sortKey="Levee, Valerie" sort="Levee, Valerie" uniqKey="Levee V" first="Valérie" last="Levee">Valérie Levee</name><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Seguin">Armand Seguin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F17 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F17 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MelampsoraV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:09-0122066
|texte= Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi
}}
| This area was generated with Dilib version V0.6.38. |  |