MelampsoraV2, PascalFrancis, Checkpoint, bibRecord, 000022

Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi

Identifieur interne : 000022 ( PascalFrancis/Checkpoint ); précédent : 000021; suivant : 000023

Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi

Auteurs : Aïda Azaiez [Canada] ; Brian Boyle [Canada] ; Valérie Levee [Canada] ; Armand Seguin [Canada]

Source :

Molecular plant-microbe interactions [ 0894-0282 ] ; 2009.

RBID : Pascal:09-0122066

Descripteurs français

Pascal (Inist)
- Transcriptome, Melampsora, Analyse quantitative, Résistance, Microbiologie, Plante.
Wicri :
- topic : Analyse quantitative.

English descriptors

KwdEn :
- Melampsora, Microbiology, Plant, Quantitative analysis, Resistance, Transcriptome.

Abstract

In natural conditions, plants are subjected to a combination of biotic stresses and often have to cope with simultaneous pathogen infections. In this report, we aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra × P. maximowiczii) to infection by two biotrophic Melampsora fungi, Melampsora larici-populina and M. medusae f. sp. deltoidae. These pathogens triggered different responses after inoculation of poplar leaves. Transcript profiling using the GeneChip Poplar Genome Array revealed a total of 416 differentially expressed transcripts whose expression level was >twofold relative to controls. Interestingly, approximately half of the differentially expressed genes in infected leaves showed altered expression following interaction with either of the Melampsora spp. We also infected poplar leaves simultaneously with both Melampsora spp. to investigate potential interaction between the responses to the individual pathogens during a mixed infection. For this mixed inoculation, the number of differentially expressed transcripts increased to 648 and our analysis showed that infection with both fungi also induced a common set of genes. The genes induced after Melampsora spp. infection were mainly related to primary and secondary metabolic processes, cell-wall reinforcement and lignification, defense and stress-related mechanisms, and signal perception and transduction.

Affiliations:

Canada

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000028
to stream PascalFrancis, to step Curation: 000102

Links to Exploration step

Pascal:09-0122066

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi</title>
<author><name sortKey="Azaiez, Aida" sort="Azaiez, Aida" uniqKey="Azaiez A" first="Aïda" last="Azaiez">Aïda Azaiez</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boyle, Brian" sort="Boyle, Brian" uniqKey="Boyle B" first="Brian" last="Boyle">Brian Boyle</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Levee, Valerie" sort="Levee, Valerie" uniqKey="Levee V" first="Valérie" last="Levee">Valérie Levee</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Seguin">Armand Seguin</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">09-0122066</idno>
<date when="2009">2009</date>
<idno type="stanalyst">PASCAL 09-0122066 INIST</idno>
<idno type="RBID">Pascal:09-0122066</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000028</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000102</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000022</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000022</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi</title>
<author><name sortKey="Azaiez, Aida" sort="Azaiez, Aida" uniqKey="Azaiez A" first="Aïda" last="Azaiez">Aïda Azaiez</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boyle, Brian" sort="Boyle, Brian" uniqKey="Boyle B" first="Brian" last="Boyle">Brian Boyle</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Levee, Valerie" sort="Levee, Valerie" uniqKey="Levee V" first="Valérie" last="Levee">Valérie Levee</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Seguin">Armand Seguin</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Quebec, QC G1V 4C7</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Molecular plant-microbe interactions</title>
<title level="j" type="abbreviated">Mol. plant-microb. interact.</title>
<idno type="ISSN">0894-0282</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Molecular plant-microbe interactions</title>
<title level="j" type="abbreviated">Mol. plant-microb. interact.</title>
<idno type="ISSN">0894-0282</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Melampsora</term>
<term>Microbiology</term>
<term>Plant</term>
<term>Quantitative analysis</term>
<term>Resistance</term>
<term>Transcriptome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Transcriptome</term>
<term>Melampsora</term>
<term>Analyse quantitative</term>
<term>Résistance</term>
<term>Microbiologie</term>
<term>Plante</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Analyse quantitative</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In natural conditions, plants are subjected to a combination of biotic stresses and often have to cope with simultaneous pathogen infections. In this report, we aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra × P. maximowiczii) to infection by two biotrophic Melampsora fungi, Melampsora larici-populina and M. medusae f. sp. deltoidae. These pathogens triggered different responses after inoculation of poplar leaves. Transcript profiling using the GeneChip Poplar Genome Array revealed a total of 416 differentially expressed transcripts whose expression level was >twofold relative to controls. Interestingly, approximately half of the differentially expressed genes in infected leaves showed altered expression following interaction with either of the Melampsora spp. We also infected poplar leaves simultaneously with both Melampsora spp. to investigate potential interaction between the responses to the individual pathogens during a mixed infection. For this mixed inoculation, the number of differentially expressed transcripts increased to 648 and our analysis showed that infection with both fungi also induced a common set of genes. The genes induced after Melampsora spp. infection were mainly related to primary and secondary metabolic processes, cell-wall reinforcement and lignification, defense and stress-related mechanisms, and signal perception and transduction.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0894-0282</s0>
</fA01>
<fA02 i1="01"><s0>MPMIEL</s0>
</fA02>
<fA03 i2="1"><s0>Mol. plant-microb. interact.</s0>
</fA03>
<fA05><s2>22</s2>
</fA05>
<fA06><s2>2</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>AZAIEZ (Aïda)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>BOYLE (Brian)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>LEVEE (Valérie)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>SEGUIN (Armand)</s1>
</fA11>
<fA14 i1="01"><s1>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy</s1>
<s2>Quebec, QC G1V 4C7</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA20><s1>190-200</s1>
</fA20>
<fA21><s1>2009</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>21598</s2>
<s5>354000184219910080</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2009 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>1 p.1/2</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>09-0122066</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Molecular plant-microbe interactions</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>In natural conditions, plants are subjected to a combination of biotic stresses and often have to cope with simultaneous pathogen infections. In this report, we aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra × P. maximowiczii) to infection by two biotrophic Melampsora fungi, Melampsora larici-populina and M. medusae f. sp. deltoidae. These pathogens triggered different responses after inoculation of poplar leaves. Transcript profiling using the GeneChip Poplar Genome Array revealed a total of 416 differentially expressed transcripts whose expression level was >twofold relative to controls. Interestingly, approximately half of the differentially expressed genes in infected leaves showed altered expression following interaction with either of the Melampsora spp. We also infected poplar leaves simultaneously with both Melampsora spp. to investigate potential interaction between the responses to the individual pathogens during a mixed infection. For this mixed inoculation, the number of differentially expressed transcripts increased to 648 and our analysis showed that infection with both fungi also induced a common set of genes. The genes induced after Melampsora spp. infection were mainly related to primary and secondary metabolic processes, cell-wall reinforcement and lignification, defense and stress-related mechanisms, and signal perception and transduction.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A34G</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Transcriptome</s0>
<s5>05</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Transcriptome</s0>
<s5>05</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Transcriptoma</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Melampsora</s0>
<s2>NS</s2>
<s5>06</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Melampsora</s0>
<s2>NS</s2>
<s5>06</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Melampsora</s0>
<s2>NS</s2>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Analyse quantitative</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Quantitative analysis</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Análisis cuantitativo</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Résistance</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Resistance</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Resistencia</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>09</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>09</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Plante</s0>
<s5>10</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Plant</s0>
<s5>10</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Planta</s0>
<s5>10</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Basidiomycota</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Basidiomycota</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Basidiomycota</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Fungi</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Fungi</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Fungi</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>089</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
<affiliations><list><country><li>Canada</li>
</country>
</list>
<tree><country name="Canada"><noRegion><name sortKey="Azaiez, Aida" sort="Azaiez, Aida" uniqKey="Azaiez A" first="Aïda" last="Azaiez">Aïda Azaiez</name>
</noRegion>
<name sortKey="Boyle, Brian" sort="Boyle, Brian" uniqKey="Boyle B" first="Brian" last="Boyle">Brian Boyle</name>
<name sortKey="Levee, Valerie" sort="Levee, Valerie" uniqKey="Levee V" first="Valérie" last="Levee">Valérie Levee</name>
<name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Seguin">Armand Seguin</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/PascalFrancis/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000022 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Checkpoint/biblio.hfd -nk 000022 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    MelampsoraV2
   |flux=    PascalFrancis
   |étape=   Checkpoint
   |type=    RBID
   |clé=     Pascal:09-0122066
   |texte=   Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi
}}

This area was generated with Dilib version V0.6.38.
Data generation: Tue Nov 24 19:18:52 2020. Site generation: Tue Nov 24 19:22:33 2020

	Serveur d'exploration Melampsora (ISTEX)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration Melampsora (ISTEX)

Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi

Transcriptome Profiling in Hybrid Poplar Following Interactions with Melampsora Rust Fungi

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri