The Wound-, Pathogen-, and Ultraviolet B-Responsive MYB134 Gene Encodes an R2R3 MYB Transcription Factor That Regulates Proanthocyanidin Synthesis in Poplar
Identifieur interne : 000F18 ( Main/Exploration ); précédent : 000F17; suivant : 000F19The Wound-, Pathogen-, and Ultraviolet B-Responsive MYB134 Gene Encodes an R2R3 MYB Transcription Factor That Regulates Proanthocyanidin Synthesis in Poplar
Auteurs : Robin D. Mellway [Canada] ; Lan T. Tran [Canada] ; Michael B. Prouse [Canada] ; Malcolm M. Campbell [Canada] ; C. Peter Constabel [Canada]Source :
- Plant physiology : (Bethesda) [ 0032-0889 ] ; 2009.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Basidiomycota (physiologie), Chromatographie en phase liquide à haute performance (MeSH), Données de séquences moléculaires (MeSH), Facteurs de transcription (composition chimique), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Flavonoïdes (métabolisme), Gènes de plante (MeSH), Liaison aux protéines (MeSH), Phylogenèse (MeSH), Phénols (métabolisme), Populus (cytologie), Populus (effets des radiations), Populus (génétique), Populus (microbiologie), Proanthocyanidines (biosynthèse), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Rayons ultraviolets (MeSH), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes végétaux (effets des radiations), Stress physiologique (effets des radiations), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Voies et réseaux métaboliques (effets des radiations).
- MESH :
- biosynthèse : Proanthocyanidines.
- composition chimique : Facteurs de transcription, Protéines végétales.
- cytologie : Populus.
- effets des radiations : Populus, Régulation de l'expression des gènes végétaux, Stress physiologique, Voies et réseaux métaboliques.
- génétique : Facteurs de transcription, Populus, Protéines végétales, Régions promotrices (génétique).
- microbiologie : Populus.
- métabolisme : Facteurs de transcription, Flavonoïdes, Phénols, Protéines végétales.
- physiologie : Basidiomycota.
- Pascal (Inist)
- Analyse de profil d'expression de gènes, Blessure, Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Gènes de plante, Liaison aux protéines, Pathogène, Phylogenèse, Rayonnement UV, Déterminisme génétique, Populus, Facteur transcription, Proanthocyanidine, Physiologie végétale, Rayons ultraviolets, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Basidiomycota (physiology), Chromatography, High Pressure Liquid (MeSH), Flavonoids (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (radiation effects), Genes, Plant (MeSH), Genetic determinism, Injury, Metabolic Networks and Pathways (radiation effects), Molecular Sequence Data (MeSH), Pathogenic, Phenols (metabolism), Phylogeny (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Plant physiology, Populus, Populus (cytology), Populus (genetics), Populus (microbiology), Populus (radiation effects), Proanthocyanidin, Proanthocyanidins (biosynthesis), Promoter Regions, Genetic (genetics), Protein Binding (MeSH), Stress, Physiological (radiation effects), Transcription Factors (chemistry), Transcription Factors (genetics), Transcription Factors (metabolism), Transcription factor, Ultraviolet Rays (MeSH), Ultraviolet radiation.
- MESH :
- chemical , biosynthesis : Proanthocyanidins.
- chemical , chemistry : Plant Proteins, Transcription Factors.
- chemical , genetics : Plant Proteins, Transcription Factors.
- chemical , metabolism : Flavonoids, Phenols, Plant Proteins, Transcription Factors.
- cytology : Populus.
- genetics : Populus, Promoter Regions, Genetic.
- microbiology : Populus.
- physiology : Basidiomycota.
- radiation effects : Gene Expression Regulation, Plant, Metabolic Networks and Pathways, Populus, Stress, Physiological.
- Amino Acid Sequence, Base Sequence, Chromatography, High Pressure Liquid, Gene Expression Profiling, Genes, Plant, Molecular Sequence Data, Phylogeny, Protein Binding, Ultraviolet Rays.
Abstract
In poplar (Populus spp.), the major defense phenolics produced in leaves are the flavonoid-derived proanthocyanidins (PAs) and the salicin-based phenolic glycosides. Transcriptional activation of PA biosynthetic genes leading to PA accumulation in leaves occurs following herbivore damage and mechanical wounding as well as infection by the fungal biotroph Melampsora medusae. In this study, we have identified a poplar R2R3 MYB transcription factor gene, MYB134, that exhibits close sequence similarity to the Arabidopsis (Arabidopsis thaliana) PA regulator TRANSPARENT TESTA2 and that is coinduced with PA biosynthetic genes following mechanical wounding, M. medusae infection, and exposure to elevated ultraviolet B light. Overexpression of MYB134 in poplar resulted in transcriptional activation of the full PA biosynthetic pathway and a significant plant-wide increase in PA levels, and electrophoretic mobility shift assays showed that recombinant MYB134 protein is able to bind to promoter regions of PA pathway genes. MYB134-overexpressing plants exhibited a concomitant reduction in phenolic glycoside concentrations and other minor alterations to levels of small phenylpropanoid metabolites. Our data provide insight into the regulatory mechanisms controlling stress-induced PA metabolism in poplar, and the identification of a regulator of stress-responsive PA biosynthesis constitutes a valuable tool for manipulating PA metabolism in poplar and investigating the biological functions of PAs in resistance to biotic and abiotic stresses.
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Mellway</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Forest Biology and Department of Biology, University of Victoria</s1><s2>Victoria, British Columbia, V8W 3N5</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Victoria, British Columbia, V8W 3N5</wicri:noRegion></affiliation></author><author><name sortKey="Tran, Lan T" sort="Tran, Lan T" uniqKey="Tran L" first="Lan T." last="Tran">Lan T. Tran</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Forest Biology and Department of Biology, University of Victoria</s1><s2>Victoria, British Columbia, V8W 3N5</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Victoria, British Columbia, V8W 3N5</wicri:noRegion></affiliation></author><author><name sortKey="Prouse, Michael B" sort="Prouse, Michael B" uniqKey="Prouse M" first="Michael B." last="Prouse">Michael B. Prouse</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Cell and Systems Biology, University of Toronto</s1><s2>Toronto, Ontario, M5S 3B2</s2><s3>CAN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Centre for the Analysis of Genome Evolution and Function, University of Toronto</s1><s2>Toronto, Ontario, M5S 3B2</s2><s3>CAN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M." last="Campbell">Malcolm M. Campbell</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Cell and Systems Biology, University of Toronto</s1><s2>Toronto, Ontario, M5S 3B2</s2><s3>CAN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Centre for the Analysis of Genome Evolution and Function, University of Toronto</s1><s2>Toronto, Ontario, M5S 3B2</s2><s3>CAN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C. Peter" last="Constabel">C. Peter Constabel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Forest Biology and Department of Biology, University of Victoria</s1><s2>Victoria, British Columbia, V8W 3N5</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Victoria, British Columbia, V8W 3N5</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant physiology : (Bethesda)</title><title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title><idno type="ISSN">0032-0889</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant physiology : (Bethesda)</title><title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title><idno type="ISSN">0032-0889</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Basidiomycota (physiology)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Flavonoids (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (radiation effects)</term><term>Genes, Plant (MeSH)</term><term>Genetic determinism</term><term>Injury</term><term>Metabolic Networks and Pathways (radiation effects)</term><term>Molecular Sequence Data (MeSH)</term><term>Pathogenic</term><term>Phenols (metabolism)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant physiology</term><term>Populus</term><term>Populus (cytology)</term><term>Populus (genetics)</term><term>Populus (microbiology)</term><term>Populus (radiation effects)</term><term>Proanthocyanidin</term><term>Proanthocyanidins (biosynthesis)</term><term>Promoter Regions, Genetic (genetics)</term><term>Protein Binding (MeSH)</term><term>Stress, Physiological (radiation effects)</term><term>Transcription Factors (chemistry)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term><term>Transcription factor</term><term>Ultraviolet Rays (MeSH)</term><term>Ultraviolet radiation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Basidiomycota (physiologie)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Facteurs de transcription (composition chimique)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (métabolisme)</term><term>Flavonoïdes (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Liaison aux protéines (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phénols (métabolisme)</term><term>Populus (cytologie)</term><term>Populus (effets des radiations)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Proanthocyanidines (biosynthèse)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Rayons ultraviolets (MeSH)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes végétaux (effets des radiations)</term><term>Stress physiologique (effets des radiations)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Voies et réseaux métaboliques (effets des radiations)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Proanthocyanidins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Flavonoids</term><term>Phenols</term><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Proanthocyanidines</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Facteurs de transcription</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term><term>Stress physiologique</term><term>Voies et réseaux métaboliques</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term><term>Populus</term><term>Protéines végétales</term><term>Régions promotrices (génétique)</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de transcription</term><term>Flavonoïdes</term><term>Phénols</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Metabolic Networks and Pathways</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Chromatography, High Pressure Liquid</term><term>Gene Expression Profiling</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Protein Binding</term><term>Ultraviolet Rays</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Blessure</term><term>Chromatographie en phase liquide à haute performance</term><term>Données de séquences moléculaires</term><term>Gènes de plante</term><term>Liaison aux protéines</term><term>Pathogène</term><term>Phylogenèse</term><term>Rayonnement UV</term><term>Déterminisme génétique</term><term>Populus</term><term>Facteur transcription</term><term>Proanthocyanidine</term><term>Physiologie végétale</term><term>Rayons ultraviolets</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In poplar (Populus spp.), the major defense phenolics produced in leaves are the flavonoid-derived proanthocyanidins (PAs) and the salicin-based phenolic glycosides. Transcriptional activation of PA biosynthetic genes leading to PA accumulation in leaves occurs following herbivore damage and mechanical wounding as well as infection by the fungal biotroph Melampsora medusae. In this study, we have identified a poplar R2R3 MYB transcription factor gene, MYB134, that exhibits close sequence similarity to the Arabidopsis (Arabidopsis thaliana) PA regulator TRANSPARENT TESTA2 and that is coinduced with PA biosynthetic genes following mechanical wounding, M. medusae infection, and exposure to elevated ultraviolet B light. Overexpression of MYB134 in poplar resulted in transcriptional activation of the full PA biosynthetic pathway and a significant plant-wide increase in PA levels, and electrophoretic mobility shift assays showed that recombinant MYB134 protein is able to bind to promoter regions of PA pathway genes. MYB134-overexpressing plants exhibited a concomitant reduction in phenolic glycoside concentrations and other minor alterations to levels of small phenylpropanoid metabolites. Our data provide insight into the regulatory mechanisms controlling stress-induced PA metabolism in poplar, and the identification of a regulator of stress-responsive PA biosynthesis constitutes a valuable tool for manipulating PA metabolism in poplar and investigating the biological functions of PAs in resistance to biotic and abiotic stresses.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><country name="Canada"><noRegion><name sortKey="Mellway, Robin D" sort="Mellway, Robin D" uniqKey="Mellway R" first="Robin D." last="Mellway">Robin D. Mellway</name></noRegion><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M." last="Campbell">Malcolm M. Campbell</name><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M." last="Campbell">Malcolm M. Campbell</name><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C. Peter" last="Constabel">C. Peter Constabel</name><name sortKey="Prouse, Michael B" sort="Prouse, Michael B" uniqKey="Prouse M" first="Michael B." last="Prouse">Michael B. Prouse</name><name sortKey="Prouse, Michael B" sort="Prouse, Michael B" uniqKey="Prouse M" first="Michael B." last="Prouse">Michael B. Prouse</name><name sortKey="Tran, Lan T" sort="Tran, Lan T" uniqKey="Tran L" first="Lan T." last="Tran">Lan T. Tran</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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