Multiple origin of metallicolous populations of the pseudometallophyte Arabidopsis halleri (Brassicaceae) in central Europe: the cpDNA testimony
Identifieur interne : 001165 ( Istex/Corpus ); précédent : 001164; suivant : 001166Multiple origin of metallicolous populations of the pseudometallophyte Arabidopsis halleri (Brassicaceae) in central Europe: the cpDNA testimony
Auteurs : Maxime Pauwels ; Pierre Saumitou-Laprade ; Anne Catherine Holl ; Daniel Petit ; Isabelle BonninSource :
- Molecular Ecology [ 0962-1083 ] ; 2005-12.
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Abstract
The population structure of the pseudo‐metallophyte herb, Arabidopsis halleri, was studied using Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism (PCR–RFLP) on chloroplast DNA (cpDNA). The history of metallicolous (M) populations showing increased zinc tolerance was investigated. Eight primer‐enzyme combinations out of 72 tested were applied to a total of 625 individuals from 28 widespread populations, 14 of them being M. Eleven distinct chlorotypes were found: five were common to nonmetallicolous (NM) and M populations, whereas six were only observed in one edaphic type (five in NM and one in M). No difference in chlorotype diversity between edaphic types was detected. Computed on the basis of chlorotype frequencies, the level of population differentiation was high but remained the same when taking into account levels of molecular divergence between chlorotypes. Isolation by distance was largely responsible for population differentiation. Geographically isolated groups of M populations were more genetically related to their closest NM populations than to each other. Our results suggest that M populations have been founded separately from distinct NM populations without suffering founding events and that the evolution towards increased tolerance observed in the distinct M population groups occurred independently.
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DOI: 10.1111/j.1365-294X.2005.02739.x
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pierre.saumitou‐laprade@univ‐lille1.fr</mods:affiliation></affiliation></author><author><name sortKey="Holl, Anne Catherine" sort="Holl, Anne Catherine" uniqKey="Holl A" first="Anne Catherine" last="Holl">Anne Catherine Holl</name><affiliation><mods:affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</mods:affiliation></affiliation></author><author><name sortKey="Petit, Daniel" sort="Petit, Daniel" uniqKey="Petit D" first="Daniel" last="Petit">Daniel Petit</name><affiliation><mods:affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</mods:affiliation></affiliation></author><author><name sortKey="Bonnin, Isabelle" sort="Bonnin, Isabelle" uniqKey="Bonnin I" first="Isabelle" last="Bonnin">Isabelle Bonnin</name><affiliation><mods:affiliation>UMR de Génétique Végétale (CNRS‐INRA‐UPS‐INAPG), Ferme du Moulon, F‐91190 Gif sur Yvette, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Ecology</title><idno type="ISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2005-12">2005-12</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">14</biblScope><biblScope unit="page" from="4403">4403</biblScope><biblScope unit="page" to="4414">4414</biblScope></imprint><idno type="ISSN">0962-1083</idno></series><idno type="istex">FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE</idno><idno type="DOI">10.1111/j.1365-294X.2005.02739.x</idno><idno type="ArticleID">MEC2739</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0962-1083</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis halleri</term><term>PCR–RFLP</term><term>cpDNA</term><term>heavy metal pollution</term><term>local adaptation</term><term>pseudometallophyte</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The population structure of the pseudo‐metallophyte herb, Arabidopsis halleri, was studied using Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism (PCR–RFLP) on chloroplast DNA (cpDNA). The history of metallicolous (M) populations showing increased zinc tolerance was investigated. Eight primer‐enzyme combinations out of 72 tested were applied to a total of 625 individuals from 28 widespread populations, 14 of them being M. Eleven distinct chlorotypes were found: five were common to nonmetallicolous (NM) and M populations, whereas six were only observed in one edaphic type (five in NM and one in M). No difference in chlorotype diversity between edaphic types was detected. Computed on the basis of chlorotype frequencies, the level of population differentiation was high but remained the same when taking into account levels of molecular divergence between chlorotypes. Isolation by distance was largely responsible for population differentiation. Geographically isolated groups of M populations were more genetically related to their closest NM populations than to each other. Our results suggest that M populations have been founded separately from distinct NM populations without suffering founding events and that the evolution towards increased tolerance observed in the distinct M population groups occurred independently.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>MAXIME PAUWELS</name><affiliations><json:string>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</json:string></affiliations></json:item><json:item><name>PIERRE SAUMITOU‐LAPRADE</name><affiliations><json:string>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</json:string><json:string>E-mail: pierre.saumitou‐laprade@univ‐lille1.fr</json:string></affiliations></json:item><json:item><name>ANNE CATHERINE HOLL</name><affiliations><json:string>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</json:string></affiliations></json:item><json:item><name>DANIEL PETIT</name><affiliations><json:string>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</json:string></affiliations></json:item><json:item><name>ISABELLE BONNIN</name><affiliations><json:string>UMR de Génétique Végétale (CNRS‐INRA‐UPS‐INAPG), Ferme du Moulon, F‐91190 Gif sur Yvette, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Arabidopsis halleri</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cpDNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>heavy metal pollution</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>local adaptation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR–RFLP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pseudometallophyte</value></json:item></subject><articleId><json:string>MEC2739</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The population structure of the pseudo‐metallophyte herb, Arabidopsis halleri, was studied using Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism (PCR–RFLP) on chloroplast DNA (cpDNA). The history of metallicolous (M) populations showing increased zinc tolerance was investigated. Eight primer‐enzyme combinations out of 72 tested were applied to a total of 625 individuals from 28 widespread populations, 14 of them being M. Eleven distinct chlorotypes were found: five were common to nonmetallicolous (NM) and M populations, whereas six were only observed in one edaphic type (five in NM and one in M). No difference in chlorotype diversity between edaphic types was detected. Computed on the basis of chlorotype frequencies, the level of population differentiation was high but remained the same when taking into account levels of molecular divergence between chlorotypes. Isolation by distance was largely responsible for population differentiation. Geographically isolated groups of M populations were more genetically related to their closest NM populations than to each other. Our results suggest that M populations have been founded separately from distinct NM populations without suffering founding events and that the evolution towards increased tolerance observed in the distinct M population groups occurred independently.</abstract><qualityIndicators><score>7.268</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595 x 782 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1352</abstractCharCount><pdfWordCount>7510</pdfWordCount><pdfCharCount>49413</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>189</abstractWordCount></qualityIndicators><title>Multiple origin of metallicolous populations of the pseudometallophyte Arabidopsis halleri (Brassicaceae) in central Europe: the cpDNA testimony</title><refBibs><json:item><author><json:item><name>SA Al‐Hiyaly</name></json:item><json:item><name>T McNeilly</name></json:item><json:item><name>AD Bradshaw</name></json:item></author><host><volume>110</volume><pages><last>580</last><first>571</first></pages><author></author><title>New Phytologist</title></host><title>The effect of zinc contamination from electricity pylons — evolution in a replicated situation</title></json:item><json:item><author><json:item><name>IA Al‐Shehbaz</name></json:item><json:item><name>SLJ O'Kane</name></json:item></author><host><author></author><title>The Arabidopsis Book</title></host><title>Taxonomy and Phylogeny of Arabidopsis (Brassicaceae)</title></json:item><json:item><author><json:item><name>J Antonovics</name></json:item><json:item><name>AD Bradshaw</name></json:item><json:item><name>RG Turner</name></json:item></author><host><volume>7</volume><pages><last>85</last><first>1</first></pages><author></author><title>Advances in Ecological Research</title></host><title>Heavy metal tolerance in plants</title></json:item><json:item><author><json:item><name>AJM Baker</name></json:item></author><host><volume>106</volume><pages><last>111</last><first>93</first></pages><issue>Suppl.</issue><author></author><title>New Phytologist</title></host><title>Metal tolerance</title></json:item><json:item><author><json:item><name>V Bert</name></json:item><json:item><name>I Bonnin</name></json:item><json:item><name>P Saumitou‐Laprade</name></json:item><json:item><name>P de Laguerie</name></json:item><json:item><name>D Petit</name></json:item></author><host><volume>155</volume><pages><last>57</last><first>47</first></pages><author></author><title>New Phytologist</title></host><title>Do Arabidopsis halleri from nonmetallicolous populations accumulate zinc and cadmium more effectively than those from metallicolous populations?</title></json:item><json:item><author><json:item><name>V Bert</name></json:item><json:item><name>MR Macnair</name></json:item><json:item><name>P de Laguerie</name></json:item><json:item><name>P Saumitou‐Laprade</name></json:item><json:item><name>D Petit</name></json:item></author><host><volume>146</volume><pages><last>233</last><first>225</first></pages><author></author><title>New Phytologist</title></host><title>Zinc tolerance and accumulation in metallicolous and non‐metallicolous populations of Arabidopsis halleri (Brassicaceae)</title></json:item><json:item><author><json:item><name>A Berton</name></json:item></author><host><volume>93</volume><pages><last>145</last><first>139</first></pages><author></author><title>Bulletin de la Société de Botanique de France</title></host><title>Presentation de plantes Arabis Halleri, Armeria elongata, Oenanthe fluviatilis, Galinsoga parviflora discoidea</title></json:item><json:item><author><json:item><name>AD Bradshaw</name></json:item></author><host><pages><last>25</last><first>1</first></pages><author></author><title>Evolutionary Ecolgy</title></host><title>The importance of evolutionary ideas in ecology and vice versa</title></json:item><json:item><author><json:item><name>AD Bradshaw</name></json:item><json:item><name>T McNeilly</name></json:item></author><host><pages><last>5</last><first>2</first></pages><author></author><title>Ecological Responses to Environmental Stresses</title></host><title>Stress tolerance in plants: the evolutionary framework</title></json:item><json:item><author><json:item><name>R Brooks</name></json:item></author><host><pages><last>202</last><first>181</first></pages><author></author><title>Plants That Hyperaccumulate Heavy Metals</title></host><title>Phytoarcheology and hyperaccumulators</title></json:item><json:item><host><author></author><title>Buse A, Norris D, Harmens H et al. 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biology</json:string></scienceMetrix></categories><publicationDate>2005</publicationDate><copyrightDate>2005</copyrightDate><doi><json:string>10.1111/j.1365-294X.2005.02739.x</json:string></doi><id>FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Multiple origin of metallicolous populations of the pseudometallophyte Arabidopsis halleri (Brassicaceae) in central Europe: the cpDNA testimony</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>WILEY</p></availability><date>2005</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Multiple origin of metallicolous populations of the pseudometallophyte Arabidopsis halleri (Brassicaceae) in central Europe: the cpDNA testimony</title><author xml:id="author-1"><persName><forename type="first">MAXIME</forename><surname>PAUWELS</surname></persName><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation></author><author xml:id="author-2"><persName><forename type="first">PIERRE</forename><surname>SAUMITOU‐LAPRADE</surname></persName><email>pierre.saumitou‐laprade@univ‐lille1.fr</email><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation></author><author xml:id="author-3"><persName><forename type="first">ANNE CATHERINE</forename><surname>HOLL</surname></persName><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation></author><author xml:id="author-4"><persName><forename type="first">DANIEL</forename><surname>PETIT</surname></persName><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation></author><author xml:id="author-5"><persName><forename type="first">ISABELLE</forename><surname>BONNIN</surname></persName><affiliation>UMR de Génétique Végétale (CNRS‐INRA‐UPS‐INAPG), Ferme du Moulon, F‐91190 Gif sur Yvette, France</affiliation></author></analytic><monogr><title level="j">Molecular Ecology</title><idno type="pISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><idno type="DOI">10.1111/(ISSN)1365-294X</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2005-12"></date><biblScope unit="volume">14</biblScope><biblScope unit="issue">14</biblScope><biblScope unit="page" from="4403">4403</biblScope><biblScope unit="page" to="4414">4414</biblScope></imprint></monogr><idno type="istex">FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE</idno><idno type="DOI">10.1111/j.1365-294X.2005.02739.x</idno><idno type="ArticleID">MEC2739</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2005</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The population structure of the pseudo‐metallophyte herb, Arabidopsis halleri, was studied using Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism (PCR–RFLP) on chloroplast DNA (cpDNA). The history of metallicolous (M) populations showing increased zinc tolerance was investigated. Eight primer‐enzyme combinations out of 72 tested were applied to a total of 625 individuals from 28 widespread populations, 14 of them being M. Eleven distinct chlorotypes were found: five were common to nonmetallicolous (NM) and M populations, whereas six were only observed in one edaphic type (five in NM and one in M). No difference in chlorotype diversity between edaphic types was detected. Computed on the basis of chlorotype frequencies, the level of population differentiation was high but remained the same when taking into account levels of molecular divergence between chlorotypes. Isolation by distance was largely responsible for population differentiation. Geographically isolated groups of M populations were more genetically related to their closest NM populations than to each other. Our results suggest that M populations have been founded separately from distinct NM populations without suffering founding events and that the evolution towards increased tolerance observed in the distinct M population groups occurred independently.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Arabidopsis halleri</term></item><item><term>cpDNA</term></item><item><term>heavy metal pollution</term></item><item><term>local adaptation</term></item><item><term>PCR–RFLP</term></item><item><term>pseudometallophyte</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2005-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-294X</doi><issn type="print">0962-1083</issn><issn type="electronic">1365-294X</issn><idGroup><id type="product" value="MEC"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="MOLECULAR ECOLOGY">Molecular Ecology</title></titleGroup></publicationMeta><publicationMeta level="part" position="12014"><doi origin="wiley">10.1111/mec.2005.14.issue-14</doi><numberingGroup><numbering type="journalVolume" number="14">14</numbering><numbering type="journalIssue" number="14">14</numbering></numberingGroup><coverDate startDate="2005-12">December 2005</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="15" status="forIssue"><doi origin="wiley">10.1111/j.1365-294X.2005.02739.x</doi><idGroup><id type="unit" value="MEC2739"></id></idGroup><countGroup><count type="pageTotal" number="12"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title><title type="tocHeading2">Phylogeography, Speciation and Hybridization</title></titleGroup><eventGroup><event type="firstOnline" date="2005-10-17"></event><event type="publishedOnlineFinalForm" date="2005-10-31"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.15 mode:FullText" date="2010-07-19"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="4403">4403</numbering><numbering type="pageLast" number="4414">4414</numbering></numberingGroup><correspondenceTo> Pierre Saumitou‐Laprade, Fax: +33 3 20436979; E‐mail: <email normalForm="pierre.saumitou-laprade@univ-lille1.fr">pierre.saumitou‐laprade@univ‐lille1.fr</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:MEC.MEC2739.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 13 May 2005; revision accepted 10 August 2005</unparsedEditorialHistory><countGroup><count type="figureTotal" number="3"></count><count type="tableTotal" number="4"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="64"></count><count type="wordTotal" number="7263"></count></countGroup><titleGroup><title type="main">Multiple origin of metallicolous populations of the pseudometallophyte <i>Arabidopsis halleri</i> (Brassicaceae) in central Europe: the cpDNA testimony</title><title type="shortAuthors">M. PAUWELS <i>ET AL.</i></title><title type="short">CPDNA DIVERSITY IN <i>ARABIDOPSIS HALLERI</i></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>MAXIME</givenNames><familyName>PAUWELS</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" corresponding="yes"><personName><givenNames>PIERRE</givenNames><familyName>SAUMITOU‐LAPRADE</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>ANNE CATHERINE</givenNames><familyName>HOLL</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>DANIEL</givenNames><familyName>PETIT</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>ISABELLE</givenNames><familyName>BONNIN</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR"><unparsedAffiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>UMR de Génétique Végétale (CNRS‐INRA‐UPS‐INAPG), Ferme du Moulon, F‐91190 Gif sur Yvette, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1"><i>Arabidopsis halleri</i></keyword><keyword xml:id="k2">cpDNA</keyword><keyword xml:id="k3">heavy metal pollution</keyword><keyword xml:id="k4">local adaptation</keyword><keyword xml:id="k5">PCR–RFLP</keyword><keyword xml:id="k6">pseudometallophyte</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The population structure of the pseudo‐metallophyte herb, <i>Arabidopsis halleri</i>, was studied using Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism (PCR–RFLP) on chloroplast DNA (cpDNA). The history of metallicolous (M) populations showing increased zinc tolerance was investigated. Eight primer‐enzyme combinations out of 72 tested were applied to a total of 625 individuals from 28 widespread populations, 14 of them being M. Eleven distinct chlorotypes were found: five were common to nonmetallicolous (NM) and M populations, whereas six were only observed in one edaphic type (five in NM and one in M). No difference in chlorotype diversity between edaphic types was detected. Computed on the basis of chlorotype frequencies, the level of population differentiation was high but remained the same when taking into account levels of molecular divergence between chlorotypes. Isolation by distance was largely responsible for population differentiation. Geographically isolated groups of M populations were more genetically related to their closest NM populations than to each other. Our results suggest that M populations have been founded separately from distinct NM populations without suffering founding events and that the evolution towards increased tolerance observed in the distinct M population groups occurred independently.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Multiple origin of metallicolous populations of the pseudometallophyte Arabidopsis halleri (Brassicaceae) in central Europe: the cpDNA testimony</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>CPDNA DIVERSITY IN ARABIDOPSIS HALLERI</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Multiple origin of metallicolous populations of the pseudometallophyte Arabidopsis halleri (Brassicaceae) in central Europe: the cpDNA testimony</title></titleInfo><name type="personal"><namePart type="given">MAXIME</namePart><namePart type="family">PAUWELS</namePart><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PIERRE</namePart><namePart type="family">SAUMITOU‐LAPRADE</namePart><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation><affiliation>E-mail: pierre.saumitou‐laprade@univ‐lille1.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ANNE CATHERINE</namePart><namePart type="family">HOLL</namePart><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DANIEL</namePart><namePart type="family">PETIT</namePart><affiliation>Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille, Bâtiment SN2, F‐59655 Villeneuve d’Ascq cedex, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ISABELLE</namePart><namePart type="family">BONNIN</namePart><affiliation>UMR de Génétique Végétale (CNRS‐INRA‐UPS‐INAPG), Ferme du Moulon, F‐91190 Gif sur Yvette, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2005-12</dateIssued><edition>Received 13 May 2005; revision accepted 10 August 2005</edition><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">3</extent><extent unit="tables">4</extent><extent unit="references">64</extent><extent unit="words">7263</extent></physicalDescription><abstract lang="en">The population structure of the pseudo‐metallophyte herb, Arabidopsis halleri, was studied using Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism (PCR–RFLP) on chloroplast DNA (cpDNA). The history of metallicolous (M) populations showing increased zinc tolerance was investigated. Eight primer‐enzyme combinations out of 72 tested were applied to a total of 625 individuals from 28 widespread populations, 14 of them being M. Eleven distinct chlorotypes were found: five were common to nonmetallicolous (NM) and M populations, whereas six were only observed in one edaphic type (five in NM and one in M). No difference in chlorotype diversity between edaphic types was detected. Computed on the basis of chlorotype frequencies, the level of population differentiation was high but remained the same when taking into account levels of molecular divergence between chlorotypes. Isolation by distance was largely responsible for population differentiation. Geographically isolated groups of M populations were more genetically related to their closest NM populations than to each other. Our results suggest that M populations have been founded separately from distinct NM populations without suffering founding events and that the evolution towards increased tolerance observed in the distinct M population groups occurred independently.</abstract><subject lang="en"><genre>keywords</genre><topic>Arabidopsis halleri</topic><topic>cpDNA</topic><topic>heavy metal pollution</topic><topic>local adaptation</topic><topic>PCR–RFLP</topic><topic>pseudometallophyte</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Ecology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0962-1083</identifier><identifier type="eISSN">1365-294X</identifier><identifier type="DOI">10.1111/(ISSN)1365-294X</identifier><identifier type="PublisherID">MEC</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>14</number></detail><detail type="issue"><caption>no.</caption><number>14</number></detail><extent unit="pages"><start>4403</start><end>4414</end><total>12</total></extent></part></relatedItem><identifier type="istex">FCFA1FCD74DE4F8B02A77D633E92ED5452C478AE</identifier><identifier type="DOI">10.1111/j.1365-294X.2005.02739.x</identifier><identifier type="ArticleID">MEC2739</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Science Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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