Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria
Identifieur interne : 003998 ( Ncbi/Merge ); précédent : 003997; suivant : 003999Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria
Auteurs : Eva C. Boritsch [France] ; Varun Khanna [France] ; Alexandre Pawlik [France] ; Nadine Honoré [France] ; Victor H. Navas [France] ; Laurence Ma [France] ; Christiane Bouchier [France] ; Torsten Seemann [Australie] ; Philip Supply [France] ; Timothy P. Stinear [Australie] ; Roland Brosch [France]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2016.
Abstract
Whereas most of the more than 130 described mycobacterial species are harmless saprophytes,
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DOI: 10.1073/pnas.1604921113
PubMed: 27528665
PubMed Central: 5024641
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Boritsch</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name><affiliation wicri:level="1"><nlm:aff id="aff2">IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pawlik, Alexandre" sort="Pawlik, Alexandre" uniqKey="Pawlik A" first="Alexandre" last="Pawlik">Alexandre Pawlik</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Honore, Nadine" sort="Honore, Nadine" uniqKey="Honore N" first="Nadine" last="Honoré">Nadine Honoré</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Navas, Victor H" sort="Navas, Victor H" uniqKey="Navas V" first="Victor H." last="Navas">Victor H. Navas</name><affiliation wicri:level="1"><nlm:aff id="aff3">IP, Lymphocyte Cell Biology Unit, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ma, Laurence" sort="Ma, Laurence" uniqKey="Ma L" first="Laurence" last="Ma">Laurence Ma</name><affiliation wicri:level="1"><nlm:aff id="aff4">IP, PF1-Plate-Forme Génomique, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bouchier, Christiane" sort="Bouchier, Christiane" uniqKey="Bouchier C" first="Christiane" last="Bouchier">Christiane Bouchier</name><affiliation wicri:level="1"><nlm:aff id="aff4">IP, PF1-Plate-Forme Génomique, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Seemann, Torsten" sort="Seemann, Torsten" uniqKey="Seemann T" first="Torsten" last="Seemann">Torsten Seemann</name><affiliation wicri:level="1"><nlm:aff id="aff5">Victorian Life Sciences Computation Initiative,<institution>University of Melbourne</institution>, Carlton, VIC 3053,<country>Australia</country>;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Supply, Philip" sort="Supply, Philip" uniqKey="Supply P" first="Philip" last="Supply">Philip Supply</name><affiliation wicri:level="1"><nlm:aff id="aff6">INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille,<institution>Université de Lille</institution>, 59000 Lille,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Stinear, Timothy P" sort="Stinear, Timothy P" uniqKey="Stinear T" first="Timothy P." last="Stinear">Timothy P. Stinear</name><affiliation wicri:level="1"><nlm:aff id="aff7">Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,<institution>University of Melbourne</institution>, Melbourne, VIC 3000,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Brosch, Roland" sort="Brosch, Roland" uniqKey="Brosch R" first="Roland" last="Brosch">Roland Brosch</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>Whereas most of the more than 130 described mycobacterial species are harmless saprophytes, <italic>Mycobacterium tuberculosis</italic>, the human tuberculosis-causing agent, represents one of the deadliest bacterial pathogens in the history of humankind. To explore the mechanisms behind this spectacular evolutionary trajectory toward pathogenicity, we have experimentally investigated the faculty of different tuberculosis-causing mycobacteria in conducting horizontal gene transfer (HGT). Our studies identified unique chromosomal DNA transfer between strains of the <italic>Mycobacterium canettii</italic> clade, which resemble most closely the putative common ancestor of the <italic>M. tuberculosis</italic> complex. This outstanding feature suggests that during the evolution of <italic>M. tuberculosis</italic>, HGT might have represented the major mechanism for acquisition of genes that helped these mycobacteria to increasingly resist host defenses and become major pathogens.</p></div></front></TEI><double pmid="27528665"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria</title><author><name sortKey="Boritsch, Eva C" sort="Boritsch, Eva C" uniqKey="Boritsch E" first="Eva C." last="Boritsch">Eva C. Boritsch</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name><affiliation wicri:level="1"><nlm:aff id="aff2">IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pawlik, Alexandre" sort="Pawlik, Alexandre" uniqKey="Pawlik A" first="Alexandre" last="Pawlik">Alexandre Pawlik</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Honore, Nadine" sort="Honore, Nadine" uniqKey="Honore N" first="Nadine" last="Honoré">Nadine Honoré</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Navas, Victor H" sort="Navas, Victor H" uniqKey="Navas V" first="Victor H." last="Navas">Victor H. Navas</name><affiliation wicri:level="1"><nlm:aff id="aff3">IP, Lymphocyte Cell Biology Unit, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ma, Laurence" sort="Ma, Laurence" uniqKey="Ma L" first="Laurence" last="Ma">Laurence Ma</name><affiliation wicri:level="1"><nlm:aff id="aff4">IP, PF1-Plate-Forme Génomique, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bouchier, Christiane" sort="Bouchier, Christiane" uniqKey="Bouchier C" first="Christiane" last="Bouchier">Christiane Bouchier</name><affiliation wicri:level="1"><nlm:aff id="aff4">IP, PF1-Plate-Forme Génomique, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Seemann, Torsten" sort="Seemann, Torsten" uniqKey="Seemann T" first="Torsten" last="Seemann">Torsten Seemann</name><affiliation wicri:level="1"><nlm:aff id="aff5">Victorian Life Sciences Computation Initiative,<institution>University of Melbourne</institution>, Carlton, VIC 3053,<country>Australia</country>;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Supply, Philip" sort="Supply, Philip" uniqKey="Supply P" first="Philip" last="Supply">Philip Supply</name><affiliation wicri:level="1"><nlm:aff id="aff6">INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille,<institution>Université de Lille</institution>, 59000 Lille,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Stinear, Timothy P" sort="Stinear, Timothy P" uniqKey="Stinear T" first="Timothy P." last="Stinear">Timothy P. Stinear</name><affiliation wicri:level="1"><nlm:aff id="aff7">Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,<institution>University of Melbourne</institution>, Melbourne, VIC 3000,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Brosch, Roland" sort="Brosch, Roland" uniqKey="Brosch R" first="Roland" last="Brosch">Roland Brosch</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27528665</idno><idno type="pmc">5024641</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024641</idno><idno type="RBID">PMC:5024641</idno><idno type="doi">10.1073/pnas.1604921113</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">001C04</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001C04</idno><idno type="wicri:Area/Pmc/Curation">001A62</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001A62</idno><idno type="wicri:Area/Pmc/Checkpoint">000819</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000819</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria</title><author><name sortKey="Boritsch, Eva C" sort="Boritsch, Eva C" uniqKey="Boritsch E" first="Eva C." last="Boritsch">Eva C. Boritsch</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name><affiliation wicri:level="1"><nlm:aff id="aff2">IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pawlik, Alexandre" sort="Pawlik, Alexandre" uniqKey="Pawlik A" first="Alexandre" last="Pawlik">Alexandre Pawlik</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Honore, Nadine" sort="Honore, Nadine" uniqKey="Honore N" first="Nadine" last="Honoré">Nadine Honoré</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Navas, Victor H" sort="Navas, Victor H" uniqKey="Navas V" first="Victor H." last="Navas">Victor H. Navas</name><affiliation wicri:level="1"><nlm:aff id="aff3">IP, Lymphocyte Cell Biology Unit, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ma, Laurence" sort="Ma, Laurence" uniqKey="Ma L" first="Laurence" last="Ma">Laurence Ma</name><affiliation wicri:level="1"><nlm:aff id="aff4">IP, PF1-Plate-Forme Génomique, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bouchier, Christiane" sort="Bouchier, Christiane" uniqKey="Bouchier C" first="Christiane" last="Bouchier">Christiane Bouchier</name><affiliation wicri:level="1"><nlm:aff id="aff4">IP, PF1-Plate-Forme Génomique, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Seemann, Torsten" sort="Seemann, Torsten" uniqKey="Seemann T" first="Torsten" last="Seemann">Torsten Seemann</name><affiliation wicri:level="1"><nlm:aff id="aff5">Victorian Life Sciences Computation Initiative,<institution>University of Melbourne</institution>, Carlton, VIC 3053,<country>Australia</country>;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Supply, Philip" sort="Supply, Philip" uniqKey="Supply P" first="Philip" last="Supply">Philip Supply</name><affiliation wicri:level="1"><nlm:aff id="aff6">INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille,<institution>Université de Lille</institution>, 59000 Lille,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Stinear, Timothy P" sort="Stinear, Timothy P" uniqKey="Stinear T" first="Timothy P." last="Stinear">Timothy P. Stinear</name><affiliation wicri:level="1"><nlm:aff id="aff7">Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,<institution>University of Melbourne</institution>, Melbourne, VIC 3000,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Brosch, Roland" sort="Brosch, Roland" uniqKey="Brosch R" first="Roland" last="Brosch">Roland Brosch</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Institut Pasteur (IP)</institution>, Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris,<country>France</country>;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>Whereas most of the more than 130 described mycobacterial species are harmless saprophytes, <italic>Mycobacterium tuberculosis</italic>, the human tuberculosis-causing agent, represents one of the deadliest bacterial pathogens in the history of humankind. To explore the mechanisms behind this spectacular evolutionary trajectory toward pathogenicity, we have experimentally investigated the faculty of different tuberculosis-causing mycobacteria in conducting horizontal gene transfer (HGT). Our studies identified unique chromosomal DNA transfer between strains of the <italic>Mycobacterium canettii</italic> clade, which resemble most closely the putative common ancestor of the <italic>M. tuberculosis</italic> complex. This outstanding feature suggests that during the evolution of <italic>M. tuberculosis</italic>, HGT might have represented the major mechanism for acquisition of genes that helped these mycobacteria to increasingly resist host defenses and become major pathogens.</p></div></front></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria.</title><author><name sortKey="Boritsch, Eva C" sort="Boritsch, Eva C" uniqKey="Boritsch E" first="Eva C" last="Boritsch">Eva C. Boritsch</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name><affiliation wicri:level="1"><nlm:affiliation>IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Pawlik, Alexandre" sort="Pawlik, Alexandre" uniqKey="Pawlik A" first="Alexandre" last="Pawlik">Alexandre Pawlik</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Honore, Nadine" sort="Honore, Nadine" uniqKey="Honore N" first="Nadine" last="Honoré">Nadine Honoré</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Navas, Victor H" sort="Navas, Victor H" uniqKey="Navas V" first="Victor H" last="Navas">Victor H. Navas</name><affiliation wicri:level="1"><nlm:affiliation>IP, Lymphocyte Cell Biology Unit, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, Lymphocyte Cell Biology Unit, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Ma, Laurence" sort="Ma, Laurence" uniqKey="Ma L" first="Laurence" last="Ma">Laurence Ma</name><affiliation wicri:level="1"><nlm:affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, PF1-Plate-Forme Génomique, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Bouchier, Christiane" sort="Bouchier, Christiane" uniqKey="Bouchier C" first="Christiane" last="Bouchier">Christiane Bouchier</name><affiliation wicri:level="1"><nlm:affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, PF1-Plate-Forme Génomique, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Seemann, Torsten" sort="Seemann, Torsten" uniqKey="Seemann T" first="Torsten" last="Seemann">Torsten Seemann</name><affiliation wicri:level="4"><nlm:affiliation>Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, VIC 3053, Australia;</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, VIC 3053</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Supply, Philip" sort="Supply, Philip" uniqKey="Supply P" first="Philip" last="Supply">Philip Supply</name><affiliation wicri:level="1"><nlm:affiliation>INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille, Université de Lille, 59000 Lille, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille, Université de Lille, 59000 Lille</wicri:regionArea><wicri:noRegion>59000 Lille</wicri:noRegion><wicri:noRegion>59000 Lille</wicri:noRegion></affiliation></author><author><name sortKey="Stinear, Timothy P" sort="Stinear, Timothy P" uniqKey="Stinear T" first="Timothy P" last="Stinear">Timothy P. Stinear</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3000</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Brosch, Roland" sort="Brosch, Roland" uniqKey="Brosch R" first="Roland" last="Brosch">Roland Brosch</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France; roland.brosch@pasteur.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27528665</idno><idno type="pmid">27528665</idno><idno type="doi">10.1073/pnas.1604921113</idno><idno type="wicri:Area/PubMed/Corpus">001A18</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A18</idno><idno type="wicri:Area/PubMed/Curation">001994</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001994</idno><idno type="wicri:Area/PubMed/Checkpoint">001994</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001994</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria.</title><author><name sortKey="Boritsch, Eva C" sort="Boritsch, Eva C" uniqKey="Boritsch E" first="Eva C" last="Boritsch">Eva C. Boritsch</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name><affiliation wicri:level="3"><nlm:affiliation>IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Pawlik, Alexandre" sort="Pawlik, Alexandre" uniqKey="Pawlik A" first="Alexandre" last="Pawlik">Alexandre Pawlik</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Honore, Nadine" sort="Honore, Nadine" uniqKey="Honore N" first="Nadine" last="Honoré">Nadine Honoré</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Navas, Victor H" sort="Navas, Victor H" uniqKey="Navas V" first="Victor H" last="Navas">Victor H. Navas</name><affiliation wicri:level="3"><nlm:affiliation>IP, Lymphocyte Cell Biology Unit, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, Lymphocyte Cell Biology Unit, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Ma, Laurence" sort="Ma, Laurence" uniqKey="Ma L" first="Laurence" last="Ma">Laurence Ma</name><affiliation wicri:level="3"><nlm:affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, PF1-Plate-Forme Génomique, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Bouchier, Christiane" sort="Bouchier, Christiane" uniqKey="Bouchier C" first="Christiane" last="Bouchier">Christiane Bouchier</name><affiliation wicri:level="3"><nlm:affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, PF1-Plate-Forme Génomique, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Seemann, Torsten" sort="Seemann, Torsten" uniqKey="Seemann T" first="Torsten" last="Seemann">Torsten Seemann</name><affiliation wicri:level="4"><nlm:affiliation>Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, VIC 3053, Australia;</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, VIC 3053</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Supply, Philip" sort="Supply, Philip" uniqKey="Supply P" first="Philip" last="Supply">Philip Supply</name><affiliation wicri:level="1"><nlm:affiliation>INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille, Université de Lille, 59000 Lille, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille, Université de Lille, 59000 Lille</wicri:regionArea><wicri:noRegion>59000 Lille</wicri:noRegion><wicri:noRegion>59000 Lille</wicri:noRegion></affiliation></author><author><name sortKey="Stinear, Timothy P" sort="Stinear, Timothy P" uniqKey="Stinear T" first="Timothy P" last="Stinear">Timothy P. Stinear</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3000</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Brosch, Roland" sort="Brosch, Roland" uniqKey="Brosch R" first="Roland" last="Brosch">Roland Brosch</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France; roland.brosch@pasteur.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a matter of debate. Here, by using fluorescence-assisted mating assays and whole genome sequencing, we present unique experimental evidence of chromosomal DNA transfer between tubercle bacilli of the early-branching Mycobacterium canettii clade. We found that the obtained recombinants had received multiple donor-derived DNA fragments in the size range of 100 bp to 118 kbp, fragments large enough to contain whole operons. Although the transfer frequency between M. canettii strains was low and no transfer could be observed among classical Mycobacterium tuberculosis complex (MTBC) strains, our study provides the proof of concept for genetic exchange in tubercle bacilli. This outstanding, now experimentally validated phenomenon presumably played a key role in the early evolution of the MTBC toward pathogenicity. Moreover, our findings also provide important information for the risk evaluation of potential transfer of drug resistance and fitness mutations among clinically relevant mycobacterial strains.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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