Head formation: OTX2 regulates Dkk1 and Lhx1 activity in the anterior mesendoderm.
Identifieur interne : 001E15 ( Ncbi/Curation ); précédent : 001E14; suivant : 001E16Head formation: OTX2 regulates Dkk1 and Lhx1 activity in the anterior mesendoderm.
Auteurs : Chi Kin Ip [Australie] ; Nicolas Fossat [Australie] ; Vanessa Jones [Australie] ; Thomas Lamonerie [France] ; Patrick P L. Tam [Australie]Source :
- Development (Cambridge, England) [ 1477-9129 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux, Cellules 3T3, Croisements génétiques, Facteurs de transcription (métabolisme), Facteurs de transcription Otx (physiologie), Luciferases (métabolisme), Mutation, Mésoderme (physiologie), Phénotype, Protéines et peptides de signalisation intercellulaire (métabolisme), Protéines à homéodomaine LIM (métabolisme), Régulation de l'expression des gènes au cours du développement, Souris, Tête (embryologie).
- MESH :
- embryologie : Tête.
- métabolisme : Facteurs de transcription, Luciferases, Protéines et peptides de signalisation intercellulaire, Protéines à homéodomaine LIM.
- physiologie : Facteurs de transcription Otx, Mésoderme.
- Animaux, Cellules 3T3, Croisements génétiques, Mutation, Phénotype, Régulation de l'expression des gènes au cours du développement, Souris.
English descriptors
- KwdEn :
- 3T3 Cells, Animals, Crosses, Genetic, Gene Expression Regulation, Developmental, Head (embryology), Intercellular Signaling Peptides and Proteins (metabolism), LIM-Homeodomain Proteins (metabolism), Luciferases (metabolism), Mesoderm (physiology), Mice, Mutation, Otx Transcription Factors (physiology), Phenotype, Transcription Factors (metabolism).
- MESH :
- chemical , metabolism : Intercellular Signaling Peptides and Proteins, LIM-Homeodomain Proteins, Luciferases, Transcription Factors.
- embryology : Head.
- physiology : Mesoderm, Otx Transcription Factors.
- 3T3 Cells, Animals, Crosses, Genetic, Gene Expression Regulation, Developmental, Mice, Mutation, Phenotype.
Abstract
The Otx2 gene encodes a paired-type homeobox transcription factor that is essential for the induction and the patterning of the anterior structures in the mouse embryo. Otx2 knockout embryos fail to form a head. Whereas previous studies have shown that Otx2 is required in the anterior visceral endoderm and the anterior neuroectoderm for head formation, its role in the anterior mesendoderm (AME) has not been assessed specifically. Here, we show that tissue-specific ablation of Otx2 in the AME phenocopies the truncation of the embryonic head of the Otx2 null mutant. Expression of Dkk1 and Lhx1, two genes that are also essential for head formation, is disrupted in the AME of the conditional Otx2-deficient embryos. Consistent with the fact that Dkk1 is a direct target of OTX2, we showed that OTX2 can interact with the H1 regulatory region of Dkk1 to activate its expression. Cross-species comparative analysis, RT-qPCR, ChIP-qPCR and luciferase assays have revealed two conserved regions in the Lhx1 locus to which OTX2 can bind to activate Lhx1 expression. Abnormal development of the embryonic head in Otx2;Lhx1 and Otx2;Dkk1 compound mutant embryos highlights the functional intersection of Otx2, Dkk1 and Lhx1 in the AME for head formation.
DOI: 10.1242/dev.114900
PubMed: 25231759
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :003431
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :003318
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003318
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001E15
Links to Exploration step
pubmed:25231759Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Head formation: OTX2 regulates Dkk1 and Lhx1 activity in the anterior mesendoderm.</title>
<author><name sortKey="Ip, Chi Kin" sort="Ip, Chi Kin" uniqKey="Ip C" first="Chi Kin" last="Ip">Chi Kin Ip</name>
<affiliation wicri:level="4"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006</wicri:regionArea>
<orgName type="university">Université de Sydney</orgName>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fossat, Nicolas" sort="Fossat, Nicolas" uniqKey="Fossat N" first="Nicolas" last="Fossat">Nicolas Fossat</name>
<affiliation wicri:level="4"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006, Australia nfossat@cmri.org.au.</nlm:affiliation>
<country wicri:rule="url">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006</wicri:regionArea>
<orgName type="university">Université de Sydney</orgName>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jones, Vanessa" sort="Jones, Vanessa" uniqKey="Jones V" first="Vanessa" last="Jones">Vanessa Jones</name>
<affiliation wicri:level="1"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145</wicri:regionArea>
<wicri:noRegion>New South Wales 2145</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lamonerie, Thomas" sort="Lamonerie, Thomas" uniqKey="Lamonerie T" first="Thomas" last="Lamonerie">Thomas Lamonerie</name>
<affiliation wicri:level="4"><nlm:affiliation>Equipe Neurodéveloppement, Institut de Biologie Valrose, UMR UNS/CNRS 7277/INSERM 1091, Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice cedex 2, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Equipe Neurodéveloppement, Institut de Biologie Valrose, UMR UNS/CNRS 7277/INSERM 1091, Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice cedex 2</wicri:regionArea>
<wicri:noRegion>06108 Nice cedex 2</wicri:noRegion>
<orgName type="university">Université Nice Sophia Antipolis</orgName>
<placeName><settlement type="city">Nice</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tam, Patrick P L" sort="Tam, Patrick P L" uniqKey="Tam P" first="Patrick P L" last="Tam">Patrick P L. Tam</name>
<affiliation wicri:level="4"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006</wicri:regionArea>
<orgName type="university">Université de Sydney</orgName>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:25231759</idno>
<idno type="pmid">25231759</idno>
<idno type="doi">10.1242/dev.114900</idno>
<idno type="wicri:Area/PubMed/Corpus">003431</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003431</idno>
<idno type="wicri:Area/PubMed/Curation">003318</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003318</idno>
<idno type="wicri:Area/PubMed/Checkpoint">003318</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003318</idno>
<idno type="wicri:Area/Ncbi/Merge">001E15</idno>
<idno type="wicri:Area/Ncbi/Curation">001E15</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Head formation: OTX2 regulates Dkk1 and Lhx1 activity in the anterior mesendoderm.</title>
<author><name sortKey="Ip, Chi Kin" sort="Ip, Chi Kin" uniqKey="Ip C" first="Chi Kin" last="Ip">Chi Kin Ip</name>
<affiliation wicri:level="4"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006</wicri:regionArea>
<orgName type="university">Université de Sydney</orgName>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fossat, Nicolas" sort="Fossat, Nicolas" uniqKey="Fossat N" first="Nicolas" last="Fossat">Nicolas Fossat</name>
<affiliation wicri:level="4"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006, Australia nfossat@cmri.org.au.</nlm:affiliation>
<country wicri:rule="url">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006</wicri:regionArea>
<orgName type="university">Université de Sydney</orgName>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jones, Vanessa" sort="Jones, Vanessa" uniqKey="Jones V" first="Vanessa" last="Jones">Vanessa Jones</name>
<affiliation wicri:level="1"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145</wicri:regionArea>
<wicri:noRegion>New South Wales 2145</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lamonerie, Thomas" sort="Lamonerie, Thomas" uniqKey="Lamonerie T" first="Thomas" last="Lamonerie">Thomas Lamonerie</name>
<affiliation wicri:level="4"><nlm:affiliation>Equipe Neurodéveloppement, Institut de Biologie Valrose, UMR UNS/CNRS 7277/INSERM 1091, Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice cedex 2, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Equipe Neurodéveloppement, Institut de Biologie Valrose, UMR UNS/CNRS 7277/INSERM 1091, Université Nice Sophia Antipolis, Parc Valrose, 06108 Nice cedex 2</wicri:regionArea>
<wicri:noRegion>06108 Nice cedex 2</wicri:noRegion>
<orgName type="university">Université Nice Sophia Antipolis</orgName>
<placeName><settlement type="city">Nice</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tam, Patrick P L" sort="Tam, Patrick P L" uniqKey="Tam P" first="Patrick P L" last="Tam">Patrick P L. Tam</name>
<affiliation wicri:level="4"><nlm:affiliation>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales 2006</wicri:regionArea>
<orgName type="university">Université de Sydney</orgName>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Development (Cambridge, England)</title>
<idno type="eISSN">1477-9129</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3T3 Cells</term>
<term>Animals</term>
<term>Crosses, Genetic</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Head (embryology)</term>
<term>Intercellular Signaling Peptides and Proteins (metabolism)</term>
<term>LIM-Homeodomain Proteins (metabolism)</term>
<term>Luciferases (metabolism)</term>
<term>Mesoderm (physiology)</term>
<term>Mice</term>
<term>Mutation</term>
<term>Otx Transcription Factors (physiology)</term>
<term>Phenotype</term>
<term>Transcription Factors (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules 3T3</term>
<term>Croisements génétiques</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Facteurs de transcription Otx (physiologie)</term>
<term>Luciferases (métabolisme)</term>
<term>Mutation</term>
<term>Mésoderme (physiologie)</term>
<term>Phénotype</term>
<term>Protéines et peptides de signalisation intercellulaire (métabolisme)</term>
<term>Protéines à homéodomaine LIM (métabolisme)</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Souris</term>
<term>Tête (embryologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Intercellular Signaling Peptides and Proteins</term>
<term>LIM-Homeodomain Proteins</term>
<term>Luciferases</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Tête</term>
</keywords>
<keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Head</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de transcription</term>
<term>Luciferases</term>
<term>Protéines et peptides de signalisation intercellulaire</term>
<term>Protéines à homéodomaine LIM</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Facteurs de transcription Otx</term>
<term>Mésoderme</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mesoderm</term>
<term>Otx Transcription Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>3T3 Cells</term>
<term>Animals</term>
<term>Crosses, Genetic</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Mice</term>
<term>Mutation</term>
<term>Phenotype</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules 3T3</term>
<term>Croisements génétiques</term>
<term>Mutation</term>
<term>Phénotype</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Souris</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The Otx2 gene encodes a paired-type homeobox transcription factor that is essential for the induction and the patterning of the anterior structures in the mouse embryo. Otx2 knockout embryos fail to form a head. Whereas previous studies have shown that Otx2 is required in the anterior visceral endoderm and the anterior neuroectoderm for head formation, its role in the anterior mesendoderm (AME) has not been assessed specifically. Here, we show that tissue-specific ablation of Otx2 in the AME phenocopies the truncation of the embryonic head of the Otx2 null mutant. Expression of Dkk1 and Lhx1, two genes that are also essential for head formation, is disrupted in the AME of the conditional Otx2-deficient embryos. Consistent with the fact that Dkk1 is a direct target of OTX2, we showed that OTX2 can interact with the H1 regulatory region of Dkk1 to activate its expression. Cross-species comparative analysis, RT-qPCR, ChIP-qPCR and luciferase assays have revealed two conserved regions in the Lhx1 locus to which OTX2 can bind to activate Lhx1 expression. Abnormal development of the embryonic head in Otx2;Lhx1 and Otx2;Dkk1 compound mutant embryos highlights the functional intersection of Otx2, Dkk1 and Lhx1 in the AME for head formation.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E15 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001E15 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:25231759 |texte= Head formation: OTX2 regulates Dkk1 and Lhx1 activity in the anterior mesendoderm. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:25231759" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
This area was generated with Dilib version V0.6.33. |