Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China
Identifieur interne : 001150 ( Ncbi/Curation ); précédent : 001149; suivant : 001151Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China
Auteurs : Belgees Boufana ; Gérald Umhang ; Jiamin Qiu ; Xingwang Chen ; Samia Lahmar ; Franck Boué ; David Jenkins ; Philip CraigSource :
- The American Journal of Tropical Medicine and Hygiene [ 0002-9637 ] ; 2013.
Abstract
To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of
Url:
DOI: 10.4269/ajtmh.12-0331
PubMed: 23438764
PubMed Central: 3617872
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001712
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :001572
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001B78
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001150
Links to Exploration step
PMC:3617872Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of Three PCR Assays for the Differentiation between <italic>Echinococcus shiquicus</italic>
, <italic>E. granulosus</italic>
(G1 genotype), and <italic>E. multilocularis</italic>
DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China</title>
<author><name sortKey="Boufana, Belgees" sort="Boufana, Belgees" uniqKey="Boufana B" first="Belgees" last="Boufana">Belgees Boufana</name>
</author>
<author><name sortKey="Umhang, Gerald" sort="Umhang, Gerald" uniqKey="Umhang G" first="Gérald" last="Umhang">Gérald Umhang</name>
</author>
<author><name sortKey="Qiu, Jiamin" sort="Qiu, Jiamin" uniqKey="Qiu J" first="Jiamin" last="Qiu">Jiamin Qiu</name>
</author>
<author><name sortKey="Chen, Xingwang" sort="Chen, Xingwang" uniqKey="Chen X" first="Xingwang" last="Chen">Xingwang Chen</name>
</author>
<author><name sortKey="Lahmar, Samia" sort="Lahmar, Samia" uniqKey="Lahmar S" first="Samia" last="Lahmar">Samia Lahmar</name>
</author>
<author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name>
</author>
<author><name sortKey="Jenkins, David" sort="Jenkins, David" uniqKey="Jenkins D" first="David" last="Jenkins">David Jenkins</name>
</author>
<author><name sortKey="Craig, Philip" sort="Craig, Philip" uniqKey="Craig P" first="Philip" last="Craig">Philip Craig</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23438764</idno>
<idno type="pmc">3617872</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617872</idno>
<idno type="RBID">PMC:3617872</idno>
<idno type="doi">10.4269/ajtmh.12-0331</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">001712</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001712</idno>
<idno type="wicri:Area/Pmc/Curation">001572</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001572</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001B78</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001B78</idno>
<idno type="wicri:Area/Ncbi/Merge">001150</idno>
<idno type="wicri:Area/Ncbi/Curation">001150</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of Three PCR Assays for the Differentiation between <italic>Echinococcus shiquicus</italic>
, <italic>E. granulosus</italic>
(G1 genotype), and <italic>E. multilocularis</italic>
DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China</title>
<author><name sortKey="Boufana, Belgees" sort="Boufana, Belgees" uniqKey="Boufana B" first="Belgees" last="Boufana">Belgees Boufana</name>
</author>
<author><name sortKey="Umhang, Gerald" sort="Umhang, Gerald" uniqKey="Umhang G" first="Gérald" last="Umhang">Gérald Umhang</name>
</author>
<author><name sortKey="Qiu, Jiamin" sort="Qiu, Jiamin" uniqKey="Qiu J" first="Jiamin" last="Qiu">Jiamin Qiu</name>
</author>
<author><name sortKey="Chen, Xingwang" sort="Chen, Xingwang" uniqKey="Chen X" first="Xingwang" last="Chen">Xingwang Chen</name>
</author>
<author><name sortKey="Lahmar, Samia" sort="Lahmar, Samia" uniqKey="Lahmar S" first="Samia" last="Lahmar">Samia Lahmar</name>
</author>
<author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name>
</author>
<author><name sortKey="Jenkins, David" sort="Jenkins, David" uniqKey="Jenkins D" first="David" last="Jenkins">David Jenkins</name>
</author>
<author><name sortKey="Craig, Philip" sort="Craig, Philip" uniqKey="Craig P" first="Philip" last="Craig">Philip Craig</name>
</author>
</analytic>
<series><title level="j">The American Journal of Tropical Medicine and Hygiene</title>
<idno type="ISSN">0002-9637</idno>
<idno type="eISSN">1476-1645</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of <italic>Echinococcus shiquicus</italic>
, <italic>Echinococcus granulosus</italic>
G1, and <italic>Echinococcus multilocularis</italic>
DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for <italic>E. shiquicus</italic>
primers that faintly detected <italic>E. equinus</italic>
DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of <italic>E. shiquicus</italic>
coproDNA using necropsy-positive fox samples.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001150 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001150 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:3617872 |texte= Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:23438764" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |