Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China
Identifieur interne : 001572 ( Pmc/Curation ); précédent : 001571; suivant : 001573Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China
Auteurs : Belgees Boufana ; Gérald Umhang ; Jiamin Qiu ; Xingwang Chen ; Samia Lahmar ; Franck Boué ; David Jenkins ; Philip CraigSource :
- The American Journal of Tropical Medicine and Hygiene [ 0002-9637 ] ; 2013.
Abstract
To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of
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DOI: 10.4269/ajtmh.12-0331
PubMed: 23438764
PubMed Central: 3617872
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of Three PCR Assays for the Differentiation between <italic>Echinococcus shiquicus</italic>, <italic>E. granulosus</italic> (G1 genotype), and <italic>E. multilocularis</italic> DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China</title><author><name sortKey="Boufana, Belgees" sort="Boufana, Belgees" uniqKey="Boufana B" first="Belgees" last="Boufana">Belgees Boufana</name></author><author><name sortKey="Umhang, Gerald" sort="Umhang, Gerald" uniqKey="Umhang G" first="Gérald" last="Umhang">Gérald Umhang</name></author><author><name sortKey="Qiu, Jiamin" sort="Qiu, Jiamin" uniqKey="Qiu J" first="Jiamin" last="Qiu">Jiamin Qiu</name></author><author><name sortKey="Chen, Xingwang" sort="Chen, Xingwang" uniqKey="Chen X" first="Xingwang" last="Chen">Xingwang Chen</name></author><author><name sortKey="Lahmar, Samia" sort="Lahmar, Samia" uniqKey="Lahmar S" first="Samia" last="Lahmar">Samia Lahmar</name></author><author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name></author><author><name sortKey="Jenkins, David" sort="Jenkins, David" uniqKey="Jenkins D" first="David" last="Jenkins">David Jenkins</name></author><author><name sortKey="Craig, Philip" sort="Craig, Philip" uniqKey="Craig P" first="Philip" last="Craig">Philip Craig</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23438764</idno><idno type="pmc">3617872</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617872</idno><idno type="RBID">PMC:3617872</idno><idno type="doi">10.4269/ajtmh.12-0331</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">001712</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001712</idno><idno type="wicri:Area/Pmc/Curation">001572</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001572</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of Three PCR Assays for the Differentiation between <italic>Echinococcus shiquicus</italic>, <italic>E. granulosus</italic> (G1 genotype), and <italic>E. multilocularis</italic> DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China</title><author><name sortKey="Boufana, Belgees" sort="Boufana, Belgees" uniqKey="Boufana B" first="Belgees" last="Boufana">Belgees Boufana</name></author><author><name sortKey="Umhang, Gerald" sort="Umhang, Gerald" uniqKey="Umhang G" first="Gérald" last="Umhang">Gérald Umhang</name></author><author><name sortKey="Qiu, Jiamin" sort="Qiu, Jiamin" uniqKey="Qiu J" first="Jiamin" last="Qiu">Jiamin Qiu</name></author><author><name sortKey="Chen, Xingwang" sort="Chen, Xingwang" uniqKey="Chen X" first="Xingwang" last="Chen">Xingwang Chen</name></author><author><name sortKey="Lahmar, Samia" sort="Lahmar, Samia" uniqKey="Lahmar S" first="Samia" last="Lahmar">Samia Lahmar</name></author><author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name></author><author><name sortKey="Jenkins, David" sort="Jenkins, David" uniqKey="Jenkins D" first="David" last="Jenkins">David Jenkins</name></author><author><name sortKey="Craig, Philip" sort="Craig, Philip" uniqKey="Craig P" first="Philip" last="Craig">Philip Craig</name></author></analytic><series><title level="j">The American Journal of Tropical Medicine and Hygiene</title><idno type="ISSN">0002-9637</idno><idno type="eISSN">1476-1645</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of <italic>Echinococcus shiquicus</italic>, <italic>Echinococcus granulosus</italic> G1, and <italic>Echinococcus multilocularis</italic> DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for <italic>E. shiquicus</italic> primers that faintly detected <italic>E. equinus</italic> DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of <italic>E. shiquicus</italic> coproDNA using necropsy-positive fox samples.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Am J Trop Med Hyg</journal-id><journal-id journal-id-type="iso-abbrev">Am. J. Trop. Med. Hyg</journal-id><journal-id journal-id-type="publisher-id">tpmd</journal-id><journal-title-group><journal-title>The American Journal of Tropical Medicine and Hygiene</journal-title></journal-title-group><issn pub-type="ppub">0002-9637</issn><issn pub-type="epub">1476-1645</issn><publisher><publisher-name>The American Society of Tropical Medicine and Hygiene</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23438764</article-id><article-id pub-id-type="pmc">3617872</article-id><article-id pub-id-type="doi">10.4269/ajtmh.12-0331</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Development of Three PCR Assays for the Differentiation between <italic>Echinococcus shiquicus</italic>, <italic>E. granulosus</italic> (G1 genotype), and <italic>E. multilocularis</italic> DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China</article-title><alt-title alt-title-type="left-running-head">BOUFANA AND OTHERS</alt-title><alt-title alt-title-type="right-running-head">DIFFERENTIATION OF <italic>E. SHIQUICUS</italic>, <italic>E. GRANULOSUS</italic>, AND <italic>E. MULTILOCULARIS</italic></alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Boufana</surname><given-names>Belgees</given-names></name><xref ref-type="corresp" rid="COR1">*</xref></contrib><contrib contrib-type="author"><name><surname>Umhang</surname><given-names>Gérald</given-names></name></contrib><contrib contrib-type="author"><name><surname>Qiu</surname><given-names>Jiamin</given-names></name></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Xingwang</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lahmar</surname><given-names>Samia</given-names></name></contrib><contrib contrib-type="author"><name><surname>Boué</surname><given-names>Franck</given-names></name></contrib><contrib contrib-type="author"><name><surname>Jenkins</surname><given-names>David</given-names></name></contrib><contrib contrib-type="author"><name><surname>Craig</surname><given-names>Philip</given-names></name></contrib></contrib-group><aff id="AFF1">Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, Salford, Greater Manchester, United Kingdom; French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Nancy Rabies and Wildlife Laboratory, Technopole Agricole et Vétérinaire, Malzéville, France; Institute of Parasitic Diseases, Sichuan Centre for Disease Control and Prevention, Chengdu, Sichuan, China; Service de Parasitologie, Ecole Nationale de Medecine Veterinaire, Sidi Thabet, Tunisia; School of Animal and Veterinary Sciences, Charles Sturt University, New South Wales, Australia</aff><author-notes><corresp id="COR1">*Address correspondence to Belgees Boufana, Cestode Zoonoses Research Group, School of Enviroment and Life Sciences, University of Salford, United Kingdom. E-mail: <email>B.Boufana@salford.ac.uk</email></corresp></author-notes><pub-date pub-type="ppub"><day>03</day><month>4</month><year>2013</year></pub-date><volume>88</volume><issue>4</issue><fpage>795</fpage><lpage>802</lpage><history><date date-type="received"><day>21</day><month>5</month><year>2012</year></date><date date-type="accepted"><day>03</day><month>12</month><year>2012</year></date></history><permissions><copyright-statement>©The American Society of Tropical Medicine and Hygiene</copyright-statement><copyright-year>2013</copyright-year></permissions><abstract><p>To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of <italic>Echinococcus shiquicus</italic>, <italic>Echinococcus granulosus</italic> G1, and <italic>Echinococcus multilocularis</italic> DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for <italic>E. shiquicus</italic> primers that faintly detected <italic>E. equinus</italic> DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of <italic>E. shiquicus</italic> coproDNA using necropsy-positive fox samples.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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