RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations
Identifieur interne : 003059 ( Ncbi/Checkpoint ); précédent : 003058; suivant : 003060RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations
Auteurs : Edin Hamzi [France, Danemark] ; Rikke Br Dsgaard Kj Rup [Danemark] ; Núria Mach [France] ; Guilietta Minozzi [Italie] ; Francesco Strozzi [Italie] ; Valentina Gualdi [Italie] ; John L. Williams [Italie, Australie] ; Jun Chen [États-Unis] ; Eva Wattrang [Suède] ; Bart Buitenhuis [Danemark] ; Helle Risdahl Juul-Madsen [Danemark] ; Tina S Rensen Dalgaard [Danemark]Source :
- BMC Genomics [ 1471-2164 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Lectine liant le mannose, Virus de la bronchite infectieuse.
- médecine vétérinaire : Infections à coronavirus.
- physiopathologie : Infections à coronavirus.
- sang : Lectine liant le mannose.
- Analyse de séquence d'ARN, Animaux, Poulets, Transcriptome.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Mannose-Binding Lectin.
- genetics : Infectious bronchitis virus, Mannose-Binding Lectin.
- physiopathology : Coronavirus Infections.
- veterinary : Coronavirus Infections.
- Animals, Chickens, Sequence Analysis, RNA, Transcriptome.
Abstract
Avian infectious bronchitis is a highly contagious disease of the upper-respiratory tract caused by infectious bronchitis virus (IBV). Understanding the molecular mechanisms involved in the interaction between innate and adaptive immune responses to IBV infection is a crucial element for further improvements in strategies to control IB. To this end, two chicken lines, selected for high (L10H line) and low (L10L line) serum concentration of mannose-binding lectin (MBL) were studied. In total, 32 birds from each line were used. Sixteen birds from each line were infected with IBV and sixteen were left uninfected. Eight uninfected and infected birds from each line were euthanized at 1 and 3 weeks post infection. RNA sequencing was performed on spleen samples from all 64 birds and differential gene expression analysis was performed for four comparisons: L10L line versus L10H line for uninfected birds at weeks 1 and 3, respectively, and in the same way for infected birds. Functional analysis was performed using Gene Ontology (GO) Immune System Process terms specific for
Comparing uninfected L10H and L10L birds, we identified 1698 and 1424 differentially expressed (DE) genes at weeks 1 and 3, respectively. For the IBV-infected birds, 1934 and 866 DE genes were identified between the two lines at weeks 1 and 3, respectively. The two most enriched GO terms emerging from the comparison of uninfected birds between the two lines were “Lymphocyte activation involved in immune response” and “Somatic recombination of immunoglobulin genes involved in immune response” at weeks 1 and 3, respectively. When comparing IBV-infected birds between the two lines, the most enriched GO terms were “Alpha-beta T cell activation” and “Positive regulation of leukocyte activation” at weeks 1 and 3, respectively.
Healthy birds from the two lines showed significant differences in expression profiles for subsets of adaptive and innate immunity-related genes, whereas comparison of the IBV-infected birds from the two lines showed differences in expression of immunity-related genes involved in T cell activation and proliferation. The observed transcriptome differences between the two lines indicate that selection for MBL had influenced innate as well as adaptive immunity.
The online version of this article (doi:10.1186/s12864-016-2403-1) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12864-016-2403-1
PubMed: 26819139
PubMed Central: 4729133
Affiliations:
- Australie, Danemark, France, Italie, Suède, États-Unis
- Arkansas, Lombardie, Île-de-France
- Jouy-en-Josas, Milan, Paris
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PMC:4729133Le document en format XML
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<affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Molecular Biology and Genetics, Center for Quantitative Genetics and Genomics, Aarhus University, Blichers Allé 20, P.O. Box 50, 8830 Tjele, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
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<author><name sortKey="Juul Madsen, Helle Risdahl" sort="Juul Madsen, Helle Risdahl" uniqKey="Juul Madsen H" first="Helle Risdahl" last="Juul-Madsen">Helle Risdahl Juul-Madsen</name>
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<author><name sortKey="Dalgaard, Tina S Rensen" sort="Dalgaard, Tina S Rensen" uniqKey="Dalgaard T" first="Tina S Rensen" last="Dalgaard">Tina S Rensen Dalgaard</name>
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<country xml:lang="fr">Danemark</country>
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<series><title level="j">BMC Genomics</title>
<idno type="eISSN">1471-2164</idno>
<imprint><date when="2016">2016</date>
</imprint>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chickens</term>
<term>Coronavirus Infections (physiopathology)</term>
<term>Coronavirus Infections (veterinary)</term>
<term>Infectious bronchitis virus (genetics)</term>
<term>Mannose-Binding Lectin (blood)</term>
<term>Mannose-Binding Lectin (genetics)</term>
<term>Sequence Analysis, RNA</term>
<term>Transcriptome</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ARN</term>
<term>Animaux</term>
<term>Infections à coronavirus (médecine vétérinaire)</term>
<term>Infections à coronavirus (physiopathologie)</term>
<term>Lectine liant le mannose (génétique)</term>
<term>Lectine liant le mannose (sang)</term>
<term>Poulets</term>
<term>Transcriptome</term>
<term>Virus de la bronchite infectieuse (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Mannose-Binding Lectin</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term>
<term>Mannose-Binding Lectin</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Lectine liant le mannose</term>
<term>Virus de la bronchite infectieuse</term>
</keywords>
<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Lectine liant le mannose</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chickens</term>
<term>Sequence Analysis, RNA</term>
<term>Transcriptome</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ARN</term>
<term>Animaux</term>
<term>Poulets</term>
<term>Transcriptome</term>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Avian infectious bronchitis is a highly contagious disease of the upper-respiratory tract caused by infectious bronchitis virus (IBV). Understanding the molecular mechanisms involved in the interaction between innate and adaptive immune responses to IBV infection is a crucial element for further improvements in strategies to control IB. To this end, two chicken lines, selected for high (L10H line) and low (L10L line) serum concentration of mannose-binding lectin (MBL) were studied. In total, 32 birds from each line were used. Sixteen birds from each line were infected with IBV and sixteen were left uninfected. Eight uninfected and infected birds from each line were euthanized at 1 and 3 weeks post infection. RNA sequencing was performed on spleen samples from all 64 birds and differential gene expression analysis was performed for four comparisons: L10L line versus L10H line for uninfected birds at weeks 1 and 3, respectively, and in the same way for infected birds. Functional analysis was performed using Gene Ontology (GO) Immune System Process terms specific for <italic>Gallus gallus</italic>
.</p>
</sec>
<sec><title>Results</title>
<p>Comparing uninfected L10H and L10L birds, we identified 1698 and 1424 differentially expressed (DE) genes at weeks 1 and 3, respectively. For the IBV-infected birds, 1934 and 866 DE genes were identified between the two lines at weeks 1 and 3, respectively. The two most enriched GO terms emerging from the comparison of uninfected birds between the two lines were “Lymphocyte activation involved in immune response” and “Somatic recombination of immunoglobulin genes involved in immune response” at weeks 1 and 3, respectively. When comparing IBV-infected birds between the two lines, the most enriched GO terms were “Alpha-beta T cell activation” and “Positive regulation of leukocyte activation” at weeks 1 and 3, respectively.</p>
</sec>
<sec><title>Conclusions</title>
<p>Healthy birds from the two lines showed significant differences in expression profiles for subsets of adaptive and innate immunity-related genes, whereas comparison of the IBV-infected birds from the two lines showed differences in expression of immunity-related genes involved in T cell activation and proliferation. The observed transcriptome differences between the two lines indicate that selection for MBL had influenced innate as well as adaptive immunity.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s12864-016-2403-1) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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<li>Danemark</li>
<li>France</li>
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<li>Suède</li>
<li>États-Unis</li>
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<region><li>Arkansas</li>
<li>Lombardie</li>
<li>Île-de-France</li>
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<settlement><li>Jouy-en-Josas</li>
<li>Milan</li>
<li>Paris</li>
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<name sortKey="Mach, Nuria" sort="Mach, Nuria" uniqKey="Mach N" first="Núria" last="Mach">Núria Mach</name>
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<name sortKey="Dalgaard, Tina S Rensen" sort="Dalgaard, Tina S Rensen" uniqKey="Dalgaard T" first="Tina S Rensen" last="Dalgaard">Tina S Rensen Dalgaard</name>
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<name sortKey="Kj Rup, Rikke Br Dsgaard" sort="Kj Rup, Rikke Br Dsgaard" uniqKey="Kj Rup R" first="Rikke Br Dsgaard" last="Kj Rup">Rikke Br Dsgaard Kj Rup</name>
</country>
<country name="Italie"><noRegion><name sortKey="Minozzi, Guilietta" sort="Minozzi, Guilietta" uniqKey="Minozzi G" first="Guilietta" last="Minozzi">Guilietta Minozzi</name>
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<name sortKey="Strozzi, Francesco" sort="Strozzi, Francesco" uniqKey="Strozzi F" first="Francesco" last="Strozzi">Francesco Strozzi</name>
<name sortKey="Williams, John L" sort="Williams, John L" uniqKey="Williams J" first="John L." last="Williams">John L. Williams</name>
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<country name="Australie"><noRegion><name sortKey="Williams, John L" sort="Williams, John L" uniqKey="Williams J" first="John L." last="Williams">John L. Williams</name>
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</country>
<country name="Suède"><noRegion><name sortKey="Wattrang, Eva" sort="Wattrang, Eva" uniqKey="Wattrang E" first="Eva" last="Wattrang">Eva Wattrang</name>
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