Oxidative DNA damage in mouse sperm chromosomes: Size matters.
Identifieur interne : 002A39 ( Main/Exploration ); précédent : 002A38; suivant : 002A40Oxidative DNA damage in mouse sperm chromosomes: Size matters.
Auteurs : Ayhan Kocer [France] ; Joelle Henry-Berger [France] ; Anais Noblanc [France] ; Alexandre Champroux [France] ; Romain Pogorelcnik [France] ; Rachel Guiton [France] ; Laurent Janny [France] ; Hanae Pons-Rejraji [France] ; Fabrice Saez [France] ; Graham D. Johnson [États-Unis] ; Stephen A. Krawetz [États-Unis] ; Juan G. Alvarez [États-Unis] ; R John Aitken [Australie] ; Joël R. Drevet [France]Source :
- Free radical biology & medicine [ 1873-4596 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- anatomopathologie : Spermatozoïdes.
- génétique : Chromosomes, Noyau de la cellule, Stress oxydatif, Éléments SINE.
- métabolisme : Spermatozoïdes.
- Altération de l'ADN, Animaux, Immunoprécipitation, Mâle, Souris, Souris de lignée C57BL, Technique FISH.
English descriptors
- KwdEn :
- MESH :
- genetics : Cell Nucleus, Chromosomes, Oxidative Stress, Short Interspersed Nucleotide Elements.
- metabolism : Spermatozoa.
- pathology : Spermatozoa.
- Animals, DNA Damage, Immunoprecipitation, In Situ Hybridization, Fluorescence, Male, Mice, Mice, Inbred C57BL.
Abstract
Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.
DOI: 10.1016/j.freeradbiomed.2015.10.419
PubMed: 26510519
Affiliations:
- Australie, France, États-Unis
- Auvergne (région administrative), Auvergne-Rhône-Alpes, Massachusetts, Michigan
- Aubière, Clermont-Ferrand
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002662
- to stream PubMed, to step Curation: 002593
- to stream PubMed, to step Checkpoint: 002593
- to stream Ncbi, to step Merge: 002C85
- to stream Ncbi, to step Curation: 002C85
- to stream Ncbi, to step Checkpoint: 002C85
- to stream Main, to step Merge: 002A39
- to stream Main, to step Curation: 002A39
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Oxidative DNA damage in mouse sperm chromosomes: Size matters.</title>
<author><name sortKey="Kocer, Ayhan" sort="Kocer, Ayhan" uniqKey="Kocer A" first="Ayhan" last="Kocer">Ayhan Kocer</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Henry Berger, Joelle" sort="Henry Berger, Joelle" uniqKey="Henry Berger J" first="Joelle" last="Henry-Berger">Joelle Henry-Berger</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Noblanc, Anais" sort="Noblanc, Anais" uniqKey="Noblanc A" first="Anais" last="Noblanc">Anais Noblanc</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Champroux, Alexandre" sort="Champroux, Alexandre" uniqKey="Champroux A" first="Alexandre" last="Champroux">Alexandre Champroux</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pogorelcnik, Romain" sort="Pogorelcnik, Romain" uniqKey="Pogorelcnik R" first="Romain" last="Pogorelcnik">Romain Pogorelcnik</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guiton, Rachel" sort="Guiton, Rachel" uniqKey="Guiton R" first="Rachel" last="Guiton">Rachel Guiton</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Janny, Laurent" sort="Janny, Laurent" uniqKey="Janny L" first="Laurent" last="Janny">Laurent Janny</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pons Rejraji, Hanae" sort="Pons Rejraji, Hanae" uniqKey="Pons Rejraji H" first="Hanae" last="Pons-Rejraji">Hanae Pons-Rejraji</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Saez, Fabrice" sort="Saez, Fabrice" uniqKey="Saez F" first="Fabrice" last="Saez">Fabrice Saez</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Johnson, Graham D" sort="Johnson, Graham D" uniqKey="Johnson G" first="Graham D" last="Johnson">Graham D. Johnson</name>
<affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201</wicri:regionArea>
<placeName><region type="state">Michigan</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Krawetz, Stephen A" sort="Krawetz, Stephen A" uniqKey="Krawetz S" first="Stephen A" last="Krawetz">Stephen A. Krawetz</name>
<affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201</wicri:regionArea>
<placeName><region type="state">Michigan</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Alvarez, Juan G" sort="Alvarez, Juan G" uniqKey="Alvarez J" first="Juan G" last="Alvarez">Juan G. Alvarez</name>
<affiliation wicri:level="2"><nlm:affiliation>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Aitken, R John" sort="Aitken, R John" uniqKey="Aitken R" first="R John" last="Aitken">R John Aitken</name>
<affiliation wicri:level="1"><nlm:affiliation>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan</wicri:regionArea>
<wicri:noRegion>Callaghan</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Drevet, Joel R" sort="Drevet, Joel R" uniqKey="Drevet J" first="Joël R" last="Drevet">Joël R. Drevet</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France. Electronic address: joel.drevet@univ-bpclermont.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2015">2015</date>
<idno type="RBID">pubmed:26510519</idno>
<idno type="pmid">26510519</idno>
<idno type="doi">10.1016/j.freeradbiomed.2015.10.419</idno>
<idno type="wicri:Area/PubMed/Corpus">002662</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002662</idno>
<idno type="wicri:Area/PubMed/Curation">002593</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002593</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002593</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002593</idno>
<idno type="wicri:Area/Ncbi/Merge">002C85</idno>
<idno type="wicri:Area/Ncbi/Curation">002C85</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002C85</idno>
<idno type="wicri:Area/Main/Merge">002A39</idno>
<idno type="wicri:Area/Main/Curation">002A39</idno>
<idno type="wicri:Area/Main/Exploration">002A39</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Oxidative DNA damage in mouse sperm chromosomes: Size matters.</title>
<author><name sortKey="Kocer, Ayhan" sort="Kocer, Ayhan" uniqKey="Kocer A" first="Ayhan" last="Kocer">Ayhan Kocer</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Aubière</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Henry Berger, Joelle" sort="Henry Berger, Joelle" uniqKey="Henry Berger J" first="Joelle" last="Henry-Berger">Joelle Henry-Berger</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Aubière</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Noblanc, Anais" sort="Noblanc, Anais" uniqKey="Noblanc A" first="Anais" last="Noblanc">Anais Noblanc</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Aubière</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Champroux, Alexandre" sort="Champroux, Alexandre" uniqKey="Champroux A" first="Alexandre" last="Champroux">Alexandre Champroux</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Aubière</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pogorelcnik, Romain" sort="Pogorelcnik, Romain" uniqKey="Pogorelcnik R" first="Romain" last="Pogorelcnik">Romain Pogorelcnik</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Aubière</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Guiton, Rachel" sort="Guiton, Rachel" uniqKey="Guiton R" first="Rachel" last="Guiton">Rachel Guiton</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Aubière</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Janny, Laurent" sort="Janny, Laurent" uniqKey="Janny L" first="Laurent" last="Janny">Laurent Janny</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pons Rejraji, Hanae" sort="Pons Rejraji, Hanae" uniqKey="Pons Rejraji H" first="Hanae" last="Pons-Rejraji">Hanae Pons-Rejraji</name>
<affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Saez, Fabrice" sort="Saez, Fabrice" uniqKey="Saez F" first="Fabrice" last="Saez">Fabrice Saez</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Johnson, Graham D" sort="Johnson, Graham D" uniqKey="Johnson G" first="Graham D" last="Johnson">Graham D. Johnson</name>
<affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201</wicri:regionArea>
<placeName><region type="state">Michigan</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Krawetz, Stephen A" sort="Krawetz, Stephen A" uniqKey="Krawetz S" first="Stephen A" last="Krawetz">Stephen A. Krawetz</name>
<affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201</wicri:regionArea>
<placeName><region type="state">Michigan</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Alvarez, Juan G" sort="Alvarez, Juan G" uniqKey="Alvarez J" first="Juan G" last="Alvarez">Juan G. Alvarez</name>
<affiliation wicri:level="2"><nlm:affiliation>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Aitken, R John" sort="Aitken, R John" uniqKey="Aitken R" first="R John" last="Aitken">R John Aitken</name>
<affiliation wicri:level="1"><nlm:affiliation>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan</wicri:regionArea>
<wicri:noRegion>Callaghan</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Drevet, Joel R" sort="Drevet, Joel R" uniqKey="Drevet J" first="Joël R" last="Drevet">Joël R. Drevet</name>
<affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France. Electronic address: joel.drevet@univ-bpclermont.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
<wicri:noRegion>63171 Aubière cedex</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Free radical biology & medicine</title>
<idno type="eISSN">1873-4596</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Nucleus (genetics)</term>
<term>Chromosomes (genetics)</term>
<term>DNA Damage</term>
<term>Immunoprecipitation</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Oxidative Stress (genetics)</term>
<term>Short Interspersed Nucleotide Elements (genetics)</term>
<term>Spermatozoa (metabolism)</term>
<term>Spermatozoa (pathology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Altération de l'ADN</term>
<term>Animaux</term>
<term>Chromosomes (génétique)</term>
<term>Immunoprécipitation</term>
<term>Mâle</term>
<term>Noyau de la cellule (génétique)</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Spermatozoïdes (anatomopathologie)</term>
<term>Spermatozoïdes (métabolisme)</term>
<term>Stress oxydatif (génétique)</term>
<term>Technique FISH</term>
<term>Éléments SINE (génétique)</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Spermatozoïdes</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Nucleus</term>
<term>Chromosomes</term>
<term>Oxidative Stress</term>
<term>Short Interspersed Nucleotide Elements</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chromosomes</term>
<term>Noyau de la cellule</term>
<term>Stress oxydatif</term>
<term>Éléments SINE</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Spermatozoa</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Spermatozoïdes</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Spermatozoa</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>DNA Damage</term>
<term>Immunoprecipitation</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Altération de l'ADN</term>
<term>Animaux</term>
<term>Immunoprécipitation</term>
<term>Mâle</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Technique FISH</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>États-Unis</li>
</country>
<region><li>Auvergne (région administrative)</li>
<li>Auvergne-Rhône-Alpes</li>
<li>Massachusetts</li>
<li>Michigan</li>
</region>
<settlement><li>Aubière</li>
<li>Clermont-Ferrand</li>
</settlement>
</list>
<tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Kocer, Ayhan" sort="Kocer, Ayhan" uniqKey="Kocer A" first="Ayhan" last="Kocer">Ayhan Kocer</name>
</region>
<name sortKey="Champroux, Alexandre" sort="Champroux, Alexandre" uniqKey="Champroux A" first="Alexandre" last="Champroux">Alexandre Champroux</name>
<name sortKey="Drevet, Joel R" sort="Drevet, Joel R" uniqKey="Drevet J" first="Joël R" last="Drevet">Joël R. Drevet</name>
<name sortKey="Guiton, Rachel" sort="Guiton, Rachel" uniqKey="Guiton R" first="Rachel" last="Guiton">Rachel Guiton</name>
<name sortKey="Henry Berger, Joelle" sort="Henry Berger, Joelle" uniqKey="Henry Berger J" first="Joelle" last="Henry-Berger">Joelle Henry-Berger</name>
<name sortKey="Janny, Laurent" sort="Janny, Laurent" uniqKey="Janny L" first="Laurent" last="Janny">Laurent Janny</name>
<name sortKey="Noblanc, Anais" sort="Noblanc, Anais" uniqKey="Noblanc A" first="Anais" last="Noblanc">Anais Noblanc</name>
<name sortKey="Pogorelcnik, Romain" sort="Pogorelcnik, Romain" uniqKey="Pogorelcnik R" first="Romain" last="Pogorelcnik">Romain Pogorelcnik</name>
<name sortKey="Pons Rejraji, Hanae" sort="Pons Rejraji, Hanae" uniqKey="Pons Rejraji H" first="Hanae" last="Pons-Rejraji">Hanae Pons-Rejraji</name>
<name sortKey="Saez, Fabrice" sort="Saez, Fabrice" uniqKey="Saez F" first="Fabrice" last="Saez">Fabrice Saez</name>
</country>
<country name="États-Unis"><region name="Michigan"><name sortKey="Johnson, Graham D" sort="Johnson, Graham D" uniqKey="Johnson G" first="Graham D" last="Johnson">Graham D. Johnson</name>
</region>
<name sortKey="Alvarez, Juan G" sort="Alvarez, Juan G" uniqKey="Alvarez J" first="Juan G" last="Alvarez">Juan G. Alvarez</name>
<name sortKey="Krawetz, Stephen A" sort="Krawetz, Stephen A" uniqKey="Krawetz S" first="Stephen A" last="Krawetz">Stephen A. Krawetz</name>
</country>
<country name="Australie"><noRegion><name sortKey="Aitken, R John" sort="Aitken, R John" uniqKey="Aitken R" first="R John" last="Aitken">R John Aitken</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A39 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002A39 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:26510519 |texte= Oxidative DNA damage in mouse sperm chromosomes: Size matters. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26510519" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |