Oxidative DNA damage in mouse sperm chromosomes: Size matters.
Identifieur interne : 002662 ( PubMed/Corpus ); précédent : 002661; suivant : 002663Oxidative DNA damage in mouse sperm chromosomes: Size matters.
Auteurs : Ayhan Kocer ; Joelle Henry-Berger ; Anais Noblanc ; Alexandre Champroux ; Romain Pogorelcnik ; Rachel Guiton ; Laurent Janny ; Hanae Pons-Rejraji ; Fabrice Saez ; Graham D. Johnson ; Stephen A. Krawetz ; Juan G. Alvarez ; R John Aitken ; Joël R. DrevetSource :
- Free radical biology & medicine [ 1873-4596 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- genetics : Cell Nucleus, Chromosomes, Oxidative Stress, Short Interspersed Nucleotide Elements.
- metabolism : Spermatozoa.
- pathology : Spermatozoa.
- Animals, DNA Damage, Immunoprecipitation, In Situ Hybridization, Fluorescence, Male, Mice, Mice, Inbred C57BL.
Abstract
Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.
DOI: 10.1016/j.freeradbiomed.2015.10.419
PubMed: 26510519
Links to Exploration step
pubmed:26510519Le document en format XML
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Johnson</name><affiliation><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Krawetz, Stephen A" sort="Krawetz, Stephen A" uniqKey="Krawetz S" first="Stephen A" last="Krawetz">Stephen A. Krawetz</name><affiliation><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Alvarez, Juan G" sort="Alvarez, Juan G" uniqKey="Alvarez J" first="Juan G" last="Alvarez">Juan G. Alvarez</name><affiliation><nlm:affiliation>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Aitken, R John" sort="Aitken, R John" uniqKey="Aitken R" first="R John" last="Aitken">R John Aitken</name><affiliation><nlm:affiliation>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Drevet, Joel R" sort="Drevet, Joel R" uniqKey="Drevet J" first="Joël R" last="Drevet">Joël R. Drevet</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France. Electronic address: joel.drevet@univ-bpclermont.fr.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26510519</idno><idno type="pmid">26510519</idno><idno type="doi">10.1016/j.freeradbiomed.2015.10.419</idno><idno type="wicri:Area/PubMed/Corpus">002662</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002662</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Oxidative DNA damage in mouse sperm chromosomes: Size matters.</title><author><name sortKey="Kocer, Ayhan" sort="Kocer, Ayhan" uniqKey="Kocer A" first="Ayhan" last="Kocer">Ayhan Kocer</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Henry Berger, Joelle" sort="Henry Berger, Joelle" uniqKey="Henry Berger J" first="Joelle" last="Henry-Berger">Joelle Henry-Berger</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Noblanc, Anais" sort="Noblanc, Anais" uniqKey="Noblanc A" first="Anais" last="Noblanc">Anais Noblanc</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Champroux, Alexandre" sort="Champroux, Alexandre" uniqKey="Champroux A" first="Alexandre" last="Champroux">Alexandre Champroux</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Pogorelcnik, Romain" sort="Pogorelcnik, Romain" uniqKey="Pogorelcnik R" first="Romain" last="Pogorelcnik">Romain Pogorelcnik</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Guiton, Rachel" sort="Guiton, Rachel" uniqKey="Guiton R" first="Rachel" last="Guiton">Rachel Guiton</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Janny, Laurent" sort="Janny, Laurent" uniqKey="Janny L" first="Laurent" last="Janny">Laurent Janny</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation></affiliation></author><author><name sortKey="Pons Rejraji, Hanae" sort="Pons Rejraji, Hanae" uniqKey="Pons Rejraji H" first="Hanae" last="Pons-Rejraji">Hanae Pons-Rejraji</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation></affiliation></author><author><name sortKey="Saez, Fabrice" sort="Saez, Fabrice" uniqKey="Saez F" first="Fabrice" last="Saez">Fabrice Saez</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Johnson, Graham D" sort="Johnson, Graham D" uniqKey="Johnson G" first="Graham D" last="Johnson">Graham D. Johnson</name><affiliation><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Krawetz, Stephen A" sort="Krawetz, Stephen A" uniqKey="Krawetz S" first="Stephen A" last="Krawetz">Stephen A. Krawetz</name><affiliation><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Alvarez, Juan G" sort="Alvarez, Juan G" uniqKey="Alvarez J" first="Juan G" last="Alvarez">Juan G. Alvarez</name><affiliation><nlm:affiliation>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Aitken, R John" sort="Aitken, R John" uniqKey="Aitken R" first="R John" last="Aitken">R John Aitken</name><affiliation><nlm:affiliation>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Drevet, Joel R" sort="Drevet, Joel R" uniqKey="Drevet J" first="Joël R" last="Drevet">Joël R. Drevet</name><affiliation><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France. Electronic address: joel.drevet@univ-bpclermont.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Free radical biology & medicine</title><idno type="eISSN">1873-4596</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Nucleus (genetics)</term><term>Chromosomes (genetics)</term><term>DNA Damage</term><term>Immunoprecipitation</term><term>In Situ Hybridization, Fluorescence</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Oxidative Stress (genetics)</term><term>Short Interspersed Nucleotide Elements (genetics)</term><term>Spermatozoa (metabolism)</term><term>Spermatozoa (pathology)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Nucleus</term><term>Chromosomes</term><term>Oxidative Stress</term><term>Short Interspersed Nucleotide Elements</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>DNA Damage</term><term>Immunoprecipitation</term><term>In Situ Hybridization, Fluorescence</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26510519</PMID><DateCreated><Year>2015</Year><Month>12</Month><Day>18</Day></DateCreated><DateCompleted><Year>2016</Year><Month>10</Month><Day>18</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4596</ISSN><JournalIssue CitedMedium="Internet"><Volume>89</Volume><PubDate><Year>2015</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Free radical biology & medicine</Title><ISOAbbreviation>Free Radic. Biol. Med.</ISOAbbreviation></Journal><ArticleTitle>Oxidative DNA damage in mouse sperm chromosomes: Size matters.</ArticleTitle><Pagination><MedlinePgn>993-1002</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.freeradbiomed.2015.10.419</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0891-5849(15)01088-6</ELocationID><Abstract><AbstractText>Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kocer</LastName><ForeName>Ayhan</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henry-Berger</LastName><ForeName>Joelle</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noblanc</LastName><ForeName>Anais</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Champroux</LastName><ForeName>Alexandre</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pogorelcnik</LastName><ForeName>Romain</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guiton</LastName><ForeName>Rachel</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Janny</LastName><ForeName>Laurent</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pons-Rejraji</LastName><ForeName>Hanae</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saez</LastName><ForeName>Fabrice</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>Graham D</ForeName><Initials>GD</Initials><AffiliationInfo><Affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krawetz</LastName><ForeName>Stephen A</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alvarez</LastName><ForeName>Juan G</ForeName><Initials>JG</Initials><AffiliationInfo><Affiliation>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aitken</LastName><ForeName>R John</ForeName><Initials>RJ</Initials><AffiliationInfo><Affiliation>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Drevet</LastName><ForeName>Joël R</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France. Electronic address: joel.drevet@univ-bpclermont.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Free Radic Biol Med</MedlineTA><NlmUniqueID>8709159</NlmUniqueID><ISSNLinking>0891-5849</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002875" MajorTopicYN="N">Chromosomes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004249" MajorTopicYN="Y">DNA Damage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D047468" MajorTopicYN="N">Immunoprecipitation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017404" MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020086" MajorTopicYN="N">Short Interspersed Nucleotide Elements</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">8-Hydroxy-2′-deoxyguanosine (8-OHdG)</Keyword><Keyword MajorTopicYN="N">Mutational risk</Keyword><Keyword MajorTopicYN="N">Nuclear organization</Keyword><Keyword MajorTopicYN="N">Oxidative stress</Keyword><Keyword MajorTopicYN="N">Sperm DNA integrity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>06</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>10</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26510519</ArticleId><ArticleId IdType="pii">S0891-5849(15)01088-6</ArticleId><ArticleId IdType="doi">10.1016/j.freeradbiomed.2015.10.419</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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