Oxidative DNA damage in mouse sperm chromosomes: Size matters.
Identifieur interne : 002C85 ( Ncbi/Curation ); précédent : 002C84; suivant : 002C86Oxidative DNA damage in mouse sperm chromosomes: Size matters.
Auteurs : Ayhan Kocer [France] ; Joelle Henry-Berger [France] ; Anais Noblanc [France] ; Alexandre Champroux [France] ; Romain Pogorelcnik [France] ; Rachel Guiton [France] ; Laurent Janny [France] ; Hanae Pons-Rejraji [France] ; Fabrice Saez [France] ; Graham D. Johnson [États-Unis] ; Stephen A. Krawetz [États-Unis] ; Juan G. Alvarez [États-Unis] ; R John Aitken [Australie] ; Joël R. Drevet [France]Source :
- Free radical biology & medicine [ 1873-4596 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- anatomopathologie : Spermatozoïdes.
- génétique : Chromosomes, Noyau de la cellule, Stress oxydatif, Éléments SINE.
- métabolisme : Spermatozoïdes.
- Altération de l'ADN, Animaux, Immunoprécipitation, Mâle, Souris, Souris de lignée C57BL, Technique FISH.
English descriptors
- KwdEn :
- MESH :
- genetics : Cell Nucleus, Chromosomes, Oxidative Stress, Short Interspersed Nucleotide Elements.
- metabolism : Spermatozoa.
- pathology : Spermatozoa.
- Animals, DNA Damage, Immunoprecipitation, In Situ Hybridization, Fluorescence, Male, Mice, Mice, Inbred C57BL.
Abstract
Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.
DOI: 10.1016/j.freeradbiomed.2015.10.419
PubMed: 26510519
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Drevet</name><affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France. 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sort="Henry Berger, Joelle" uniqKey="Henry Berger J" first="Joelle" last="Henry-Berger">Joelle Henry-Berger</name><affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Aubière</settlement></placeName></affiliation></author><author><name sortKey="Noblanc, Anais" sort="Noblanc, Anais" uniqKey="Noblanc A" first="Anais" last="Noblanc">Anais Noblanc</name><affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Aubière</settlement></placeName></affiliation></author><author><name sortKey="Champroux, Alexandre" sort="Champroux, Alexandre" uniqKey="Champroux A" first="Alexandre" last="Champroux">Alexandre Champroux</name><affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Aubière</settlement></placeName></affiliation></author><author><name sortKey="Pogorelcnik, Romain" sort="Pogorelcnik, Romain" uniqKey="Pogorelcnik R" first="Romain" last="Pogorelcnik">Romain Pogorelcnik</name><affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Aubière</settlement></placeName></affiliation></author><author><name sortKey="Guiton, Rachel" sort="Guiton, Rachel" uniqKey="Guiton R" first="Rachel" last="Guiton">Rachel Guiton</name><affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Aubière</settlement></placeName></affiliation></author><author><name sortKey="Janny, Laurent" sort="Janny, Laurent" uniqKey="Janny L" first="Laurent" last="Janny">Laurent Janny</name><affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Auvergne (région administrative)</region><settlement type="city">Clermont-Ferrand</settlement></placeName></affiliation></author><author><name sortKey="Pons Rejraji, Hanae" sort="Pons Rejraji, Hanae" uniqKey="Pons Rejraji H" first="Hanae" last="Pons-Rejraji">Hanae Pons-Rejraji</name><affiliation wicri:level="3"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France; CHU Estaing, Assistance Médicale à la Procréation, Clermont-Ferrand</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Auvergne (région administrative)</region><settlement type="city">Clermont-Ferrand</settlement></placeName></affiliation></author><author><name sortKey="Saez, Fabrice" sort="Saez, Fabrice" uniqKey="Saez F" first="Fabrice" last="Saez">Fabrice Saez</name><affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea><wicri:noRegion>63171 Aubière cedex</wicri:noRegion><wicri:noRegion>63171 Aubière cedex</wicri:noRegion></affiliation></author><author><name sortKey="Johnson, Graham D" sort="Johnson, Graham D" uniqKey="Johnson G" first="Graham D" last="Johnson">Graham D. Johnson</name><affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Krawetz, Stephen A" sort="Krawetz, Stephen A" uniqKey="Krawetz S" first="Stephen A" last="Krawetz">Stephen A. Krawetz</name><affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Molecular Medicine & Genetics, Department of Obstetrics & Gynecology, Wayne State University, Detroit, MI 48201</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Alvarez, Juan G" sort="Alvarez, Juan G" uniqKey="Alvarez J" first="Juan G" last="Alvarez">Juan G. Alvarez</name><affiliation wicri:level="2"><nlm:affiliation>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centro ANDROGEN, La Coruña, Spain; Harvard Medical School, Boston, MA 02115</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Aitken, R John" sort="Aitken, R John" uniqKey="Aitken R" first="R John" last="Aitken">R John Aitken</name><affiliation wicri:level="1"><nlm:affiliation>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan</wicri:regionArea><wicri:noRegion>Callaghan</wicri:noRegion></affiliation></author><author><name sortKey="Drevet, Joel R" sort="Drevet, Joel R" uniqKey="Drevet J" first="Joël R" last="Drevet">Joël R. Drevet</name><affiliation wicri:level="1"><nlm:affiliation>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex, France. Electronic address: joel.drevet@univ-bpclermont.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>GReD laboratory, CNRS UMR6293-INSERM U1103-Clermont Université, BP80006, 63171 Aubière cedex</wicri:regionArea><wicri:noRegion>63171 Aubière cedex</wicri:noRegion><wicri:noRegion>63171 Aubière cedex</wicri:noRegion></affiliation></author></analytic><series><title level="j">Free radical biology & medicine</title><idno type="eISSN">1873-4596</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Nucleus (genetics)</term><term>Chromosomes (genetics)</term><term>DNA Damage</term><term>Immunoprecipitation</term><term>In Situ Hybridization, Fluorescence</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Oxidative Stress (genetics)</term><term>Short Interspersed Nucleotide Elements (genetics)</term><term>Spermatozoa (metabolism)</term><term>Spermatozoa (pathology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Altération de l'ADN</term><term>Animaux</term><term>Chromosomes (génétique)</term><term>Immunoprécipitation</term><term>Mâle</term><term>Noyau de la cellule (génétique)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Spermatozoïdes (anatomopathologie)</term><term>Spermatozoïdes (métabolisme)</term><term>Stress oxydatif (génétique)</term><term>Technique FISH</term><term>Éléments SINE (génétique)</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Spermatozoïdes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Nucleus</term><term>Chromosomes</term><term>Oxidative Stress</term><term>Short Interspersed Nucleotide Elements</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chromosomes</term><term>Noyau de la cellule</term><term>Stress oxydatif</term><term>Éléments SINE</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Spermatozoïdes</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>DNA Damage</term><term>Immunoprecipitation</term><term>In Situ Hybridization, Fluorescence</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Altération de l'ADN</term><term>Animaux</term><term>Immunoprécipitation</term><term>Mâle</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Technique FISH</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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