AustralieFrV1, Main, Exploration, bibRecord, 004755

Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China

Identifieur interne : 004755 ( Main/Exploration ); précédent : 004754; suivant : 004756

Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China

Auteurs : Belgees Boufana ; Gérald Umhang ; Jiamin Qiu ; Xingwang Chen ; Samia Lahmar ; Franck Boué ; David Jenkins ; Philip Craig

Source :

The American Journal of Tropical Medicine and Hygiene [ 0002-9637 ] ; 2013.

RBID : PMC:3617872

Abstract

To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of Echinococcus shiquicus, Echinococcus granulosus G1, and Echinococcus multilocularis DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for E. shiquicus primers that faintly detected E. equinus DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of E. shiquicus coproDNA using necropsy-positive fox samples.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617872

DOI: 10.4269/ajtmh.12-0331
PubMed: 23438764
PubMed Central: 3617872

Affiliations:

Le document en format XML

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 (G1 genotype), and <italic>E. multilocularis</italic>
 DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China</title>
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<author><name sortKey="Qiu, Jiamin" sort="Qiu, Jiamin" uniqKey="Qiu J" first="Jiamin" last="Qiu">Jiamin Qiu</name>
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<author><name sortKey="Chen, Xingwang" sort="Chen, Xingwang" uniqKey="Chen X" first="Xingwang" last="Chen">Xingwang Chen</name>
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<author><name sortKey="Lahmar, Samia" sort="Lahmar, Samia" uniqKey="Lahmar S" first="Samia" last="Lahmar">Samia Lahmar</name>
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<author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name>
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<author><name sortKey="Jenkins, David" sort="Jenkins, David" uniqKey="Jenkins D" first="David" last="Jenkins">David Jenkins</name>
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<author><name sortKey="Craig, Philip" sort="Craig, Philip" uniqKey="Craig P" first="Philip" last="Craig">Philip Craig</name>
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 (G1 genotype), and <italic>E. multilocularis</italic>
 DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China</title>
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<author><name sortKey="Lahmar, Samia" sort="Lahmar, Samia" uniqKey="Lahmar S" first="Samia" last="Lahmar">Samia Lahmar</name>
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<author><name sortKey="Jenkins, David" sort="Jenkins, David" uniqKey="Jenkins D" first="David" last="Jenkins">David Jenkins</name>
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<author><name sortKey="Craig, Philip" sort="Craig, Philip" uniqKey="Craig P" first="Philip" last="Craig">Philip Craig</name>
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<series><title level="j">The American Journal of Tropical Medicine and Hygiene</title>
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<front><div type="abstract" xml:lang="en"><p>To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of <italic>Echinococcus shiquicus</italic>
, <italic>Echinococcus granulosus</italic>
 G1, and <italic>Echinococcus multilocularis</italic>
 DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for <italic>E. shiquicus</italic>
 primers that faintly detected <italic>E. equinus</italic>
 DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of <italic>E. shiquicus</italic>
 coproDNA using necropsy-positive fox samples.</p>
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<tree><noCountry><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name>
<name sortKey="Boufana, Belgees" sort="Boufana, Belgees" uniqKey="Boufana B" first="Belgees" last="Boufana">Belgees Boufana</name>
<name sortKey="Chen, Xingwang" sort="Chen, Xingwang" uniqKey="Chen X" first="Xingwang" last="Chen">Xingwang Chen</name>
<name sortKey="Craig, Philip" sort="Craig, Philip" uniqKey="Craig P" first="Philip" last="Craig">Philip Craig</name>
<name sortKey="Jenkins, David" sort="Jenkins, David" uniqKey="Jenkins D" first="David" last="Jenkins">David Jenkins</name>
<name sortKey="Lahmar, Samia" sort="Lahmar, Samia" uniqKey="Lahmar S" first="Samia" last="Lahmar">Samia Lahmar</name>
<name sortKey="Qiu, Jiamin" sort="Qiu, Jiamin" uniqKey="Qiu J" first="Jiamin" last="Qiu">Jiamin Qiu</name>
<name sortKey="Umhang, Gerald" sort="Umhang, Gerald" uniqKey="Umhang G" first="Gérald" last="Umhang">Gérald Umhang</name>
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Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China

Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China

Source :

Abstract

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