Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat
Identifieur interne : 001042 ( Istex/Corpus ); précédent : 001041; suivant : 001043Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat
Auteurs : Etienne Paux ; Sébastien Faure ; Frédéric Choulet ; Delphine Roger ; Valérie Gauthier ; Jean-Pierre Martinant ; Pierre Sourdille ; François Balfourier ; Marie-Christine Le Paslier ; Aurélie Chauveau ; Mehmet Cakir ; Béatrice Gandon ; Catherine FeuilletSource :
- Plant Biotechnology Journal [ 1467-7644 ] ; 2010-02.
English descriptors
- KwdEn :
- Allele, Amplicon, Aneuploid, Anking, Appl, Assay, Biol, Biosystems, Biotechnology, Blackwell publishing, Bread wheat, Chinese spring, Chinese spring renan, Chromosome, Common wheat, Context sequence, Context sequences, Courtot, Courtot chinese spring, Curve analysis, Data points, Deletion, Deletion lines, Detection methods, Etienne, Etienne paux, European lines, Feuillet, Genet, Genetic, Genetic diversity, Genetic maps, Genetics, Genome, Genome saturation, Genomic, Genotype, Genotyping, Goldengate, Gupta, Hexaploid, Hexaploid wheat, High density, High level, Illumina, Illumina beadarray technology, Illumina goldengate assay, Inra, Inra journal compilation, Insertion, Insertion polymorphism, Isbp, Isbp markers, Isbpfinder, Isbps, Junction, Locus, Maize, Marker, Molecular markers, Multiplex system, Mutation, Paux, Plant biotechnology journal, Plant physiol, Polymorphic markers, Polymorphism, Presence absence, Presence absence polymorphism, Primer, Programme, Query, Renan, Saintenac, Same time, Sequence polymorphism, Sequencing, Snapshot, Snp, Sourdille, Success rate, Target loci, Theor, Throughput, Transposable, Transposable elements, Triticum aestivum, Unpublished data, Uorescence, Wheat, Wheat genome, Wheat lines, Worldwide bread wheat core collection.
- Teeft :
- Allele, Amplicon, Aneuploid, Anking, Appl, Assay, Biol, Biosystems, Biotechnology, Blackwell publishing, Bread wheat, Chinese spring, Chinese spring renan, Chromosome, Common wheat, Context sequence, Context sequences, Courtot, Courtot chinese spring, Curve analysis, Data points, Deletion, Deletion lines, Detection methods, Etienne, Etienne paux, European lines, Feuillet, Genet, Genetic, Genetic diversity, Genetic maps, Genetics, Genome, Genome saturation, Genomic, Genotype, Genotyping, Goldengate, Gupta, Hexaploid, Hexaploid wheat, High density, High level, Illumina, Illumina beadarray technology, Illumina goldengate assay, Inra, Inra journal compilation, Insertion, Insertion polymorphism, Isbp, Isbp markers, Isbpfinder, Isbps, Junction, Locus, Maize, Marker, Molecular markers, Multiplex system, Mutation, Paux, Plant biotechnology journal, Plant physiol, Polymorphic markers, Polymorphism, Presence absence, Presence absence polymorphism, Primer, Programme, Query, Renan, Saintenac, Same time, Sequence polymorphism, Sequencing, Snapshot, Snp, Sourdille, Success rate, Target loci, Theor, Throughput, Transposable, Transposable elements, Triticum aestivum, Unpublished data, Uorescence, Wheat, Wheat genome, Wheat lines, Worldwide bread wheat core collection.
Abstract
In wheat, the deployment of marker‐assisted selection has long been hampered by the lack of markers compatible with high‐throughput cost‐effective genotyping techniques. Recently, insertion site‐based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high‐throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, IsbpFinder, for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot® Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP‐derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost‐effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker‐assisted selection.
Url:
DOI: 10.1111/j.1467-7652.2009.00477.x
Links to Exploration step
ISTEX:5759BA874BA5C2AA9AD30274B75D8426174CF785Le document en format XML
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wheat</title><author><name sortKey="Paux, Etienne" sort="Paux, Etienne" uniqKey="Paux E" first="Etienne" last="Paux">Etienne Paux</name><affiliation><mods:affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: etienne.paux@clermont.inra.fr</mods:affiliation></affiliation></author><author><name sortKey="Faure, Sebastien" sort="Faure, Sebastien" uniqKey="Faure S" first="Sébastien" last="Faure">Sébastien Faure</name><affiliation><mods:affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</mods:affiliation></affiliation></author><author><name sortKey="Choulet, Frederic" sort="Choulet, Frederic" uniqKey="Choulet F" first="Frédéric" last="Choulet">Frédéric Choulet</name><affiliation><mods:affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</mods:affiliation></affiliation></author><author><name sortKey="Roger, Delphine" sort="Roger, Delphine" uniqKey="Roger D" first="Delphine" last="Roger">Delphine Roger</name><affiliation><mods:affiliation>Limagrain Verneuil Holding, Riom cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Gauthier, Valerie" sort="Gauthier, Valerie" uniqKey="Gauthier V" first="Valérie" last="Gauthier">Valérie Gauthier</name><affiliation><mods:affiliation>Limagrain Verneuil Holding, Riom cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Martinant, Jean Ierre" sort="Martinant, Jean Ierre" uniqKey="Martinant J" first="Jean-Pierre" last="Martinant">Jean-Pierre Martinant</name><affiliation><mods:affiliation>Limagrain Verneuil Holding, Riom cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Sourdille, Pierre" sort="Sourdille, Pierre" uniqKey="Sourdille P" first="Pierre" last="Sourdille">Pierre 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Chauveau</name><affiliation><mods:affiliation>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Cakir, Mehmet" sort="Cakir, Mehmet" uniqKey="Cakir M" first="Mehmet" last="Cakir">Mehmet Cakir</name><affiliation><mods:affiliation>School of Biological Sciences & Biotechnology, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia</mods:affiliation></affiliation></author><author><name sortKey="Gandon, Beatrice" sort="Gandon, Beatrice" uniqKey="Gandon B" first="Béatrice" last="Gandon">Béatrice Gandon</name><affiliation><mods:affiliation>Limagrain Verneuil Holding, Riom cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Feuillet, Catherine" sort="Feuillet, Catherine" uniqKey="Feuillet C" first="Catherine" last="Feuillet">Catherine Feuillet</name><affiliation><mods:affiliation>INRA UBP UMR 1095, 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xml:lang="en"><term>Allele</term><term>Amplicon</term><term>Aneuploid</term><term>Anking</term><term>Appl</term><term>Assay</term><term>Biol</term><term>Biosystems</term><term>Biotechnology</term><term>Blackwell publishing</term><term>Bread wheat</term><term>Chinese spring</term><term>Chinese spring renan</term><term>Chromosome</term><term>Common wheat</term><term>Context sequence</term><term>Context sequences</term><term>Courtot</term><term>Courtot chinese spring</term><term>Curve analysis</term><term>Data points</term><term>Deletion</term><term>Deletion lines</term><term>Detection methods</term><term>Etienne</term><term>Etienne paux</term><term>European lines</term><term>Feuillet</term><term>Genet</term><term>Genetic</term><term>Genetic diversity</term><term>Genetic maps</term><term>Genetics</term><term>Genome</term><term>Genome saturation</term><term>Genomic</term><term>Genotype</term><term>Genotyping</term><term>Goldengate</term><term>Gupta</term><term>Hexaploid</term><term>Hexaploid wheat</term><term>High density</term><term>High level</term><term>Illumina</term><term>Illumina beadarray technology</term><term>Illumina goldengate assay</term><term>Inra</term><term>Inra journal compilation</term><term>Insertion</term><term>Insertion polymorphism</term><term>Isbp</term><term>Isbp markers</term><term>Isbpfinder</term><term>Isbps</term><term>Junction</term><term>Locus</term><term>Maize</term><term>Marker</term><term>Molecular markers</term><term>Multiplex system</term><term>Mutation</term><term>Paux</term><term>Plant biotechnology journal</term><term>Plant physiol</term><term>Polymorphic markers</term><term>Polymorphism</term><term>Presence absence</term><term>Presence absence polymorphism</term><term>Primer</term><term>Programme</term><term>Query</term><term>Renan</term><term>Saintenac</term><term>Same time</term><term>Sequence polymorphism</term><term>Sequencing</term><term>Snapshot</term><term>Snp</term><term>Sourdille</term><term>Success rate</term><term>Target loci</term><term>Theor</term><term>Throughput</term><term>Transposable</term><term>Transposable elements</term><term>Triticum aestivum</term><term>Unpublished data</term><term>Uorescence</term><term>Wheat</term><term>Wheat genome</term><term>Wheat lines</term><term>Worldwide bread wheat core collection</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Allele</term><term>Amplicon</term><term>Aneuploid</term><term>Anking</term><term>Appl</term><term>Assay</term><term>Biol</term><term>Biosystems</term><term>Biotechnology</term><term>Blackwell publishing</term><term>Bread wheat</term><term>Chinese spring</term><term>Chinese spring renan</term><term>Chromosome</term><term>Common wheat</term><term>Context sequence</term><term>Context sequences</term><term>Courtot</term><term>Courtot chinese spring</term><term>Curve analysis</term><term>Data points</term><term>Deletion</term><term>Deletion lines</term><term>Detection methods</term><term>Etienne</term><term>Etienne paux</term><term>European lines</term><term>Feuillet</term><term>Genet</term><term>Genetic</term><term>Genetic diversity</term><term>Genetic maps</term><term>Genetics</term><term>Genome</term><term>Genome saturation</term><term>Genomic</term><term>Genotype</term><term>Genotyping</term><term>Goldengate</term><term>Gupta</term><term>Hexaploid</term><term>Hexaploid wheat</term><term>High density</term><term>High level</term><term>Illumina</term><term>Illumina beadarray technology</term><term>Illumina goldengate assay</term><term>Inra</term><term>Inra journal compilation</term><term>Insertion</term><term>Insertion polymorphism</term><term>Isbp</term><term>Isbp markers</term><term>Isbpfinder</term><term>Isbps</term><term>Junction</term><term>Locus</term><term>Maize</term><term>Marker</term><term>Molecular markers</term><term>Multiplex system</term><term>Mutation</term><term>Paux</term><term>Plant biotechnology journal</term><term>Plant physiol</term><term>Polymorphic markers</term><term>Polymorphism</term><term>Presence absence</term><term>Presence absence polymorphism</term><term>Primer</term><term>Programme</term><term>Query</term><term>Renan</term><term>Saintenac</term><term>Same time</term><term>Sequence polymorphism</term><term>Sequencing</term><term>Snapshot</term><term>Snp</term><term>Sourdille</term><term>Success rate</term><term>Target loci</term><term>Theor</term><term>Throughput</term><term>Transposable</term><term>Transposable elements</term><term>Triticum aestivum</term><term>Unpublished data</term><term>Uorescence</term><term>Wheat</term><term>Wheat genome</term><term>Wheat lines</term><term>Worldwide bread wheat core collection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In wheat, the deployment of marker‐assisted selection has long been hampered by the lack of markers compatible with high‐throughput cost‐effective genotyping techniques. Recently, insertion site‐based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high‐throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, IsbpFinder, for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot® Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP‐derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost‐effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker‐assisted selection.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>polymorphism</json:string><json:string>isbps</json:string><json:string>isbp</json:string><json:string>genome</json:string><json:string>genotyping</json:string><json:string>paux</json:string><json:string>chromosome</json:string><json:string>isbp markers</json:string><json:string>programme</json:string><json:string>primer</json:string><json:string>illumina</json:string><json:string>genet</json:string><json:string>inra</json:string><json:string>hexaploid</json:string><json:string>snp</json:string><json:string>sequencing</json:string><json:string>biotechnology</json:string><json:string>inra journal compilation</json:string><json:string>isbpfinder</json:string><json:string>allele</json:string><json:string>appl</json:string><json:string>blackwell 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Paux</name><affiliations><json:string>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</json:string><json:string>E-mail: etienne.paux@clermont.inra.fr</json:string></affiliations></json:item><json:item><name>Sébastien Faure</name><affiliations><json:string>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</json:string></affiliations></json:item><json:item><name>Frédéric Choulet</name><affiliations><json:string>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</json:string></affiliations></json:item><json:item><name>Delphine Roger</name><affiliations><json:string>Limagrain Verneuil Holding, Riom cedex, France</json:string></affiliations></json:item><json:item><name>Valérie Gauthier</name><affiliations><json:string>Limagrain Verneuil Holding, Riom cedex, France</json:string></affiliations></json:item><json:item><name>Jean‐Pierre Martinant</name><affiliations><json:string>Limagrain Verneuil Holding, Riom cedex, France</json:string></affiliations></json:item><json:item><name>Pierre Sourdille</name><affiliations><json:string>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</json:string></affiliations></json:item><json:item><name>François Balfourier</name><affiliations><json:string>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</json:string></affiliations></json:item><json:item><name>Marie‐Christine Le Paslier</name><affiliations><json:string>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France</json:string></affiliations></json:item><json:item><name>Aurélie Chauveau</name><affiliations><json:string>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France</json:string></affiliations></json:item><json:item><name>Mehmet Cakir</name><affiliations><json:string>School of Biological Sciences & Biotechnology, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia</json:string></affiliations></json:item><json:item><name>Béatrice Gandon</name><affiliations><json:string>Limagrain Verneuil Holding, Riom cedex, France</json:string></affiliations></json:item><json:item><name>Catherine Feuillet</name><affiliations><json:string>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Insertion site‐based polymorphism</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>molecular marker</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>polymorphism</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>mapping</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>marker‐assisted selection</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>wheat</value></json:item></subject><articleId><json:string>PBI477</json:string></articleId><arkIstex>ark:/67375/WNG-XCMZRD14-C</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>In wheat, the deployment of marker‐assisted selection has long been hampered by the lack of markers compatible with high‐throughput cost‐effective genotyping techniques. Recently, insertion site‐based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high‐throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, IsbpFinder, for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot® Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP‐derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost‐effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker‐assisted selection.</abstract><qualityIndicators><score>9.4</score><pdfWordCount>9264</pdfWordCount><pdfCharCount>57455</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>15</pdfPageCount><pdfPageSize>595.276 x 819.213 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>200</abstractWordCount><abstractCharCount>1390</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat</title><pmid><json:string>20078842</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Plant Biotechnology Journal</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1467-7652</json:string></doi><issn><json:string>1467-7644</json:string></issn><eissn><json:string>1467-7652</json:string></eissn><publisherId><json:string>PBI</json:string></publisherId><volume>8</volume><issue>2</issue><pages><first>196</first><last>210</last><total>15</total></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>the 21st century</json:string><json:string>3753</json:string><json:string>1981</json:string><json:string>4847</json:string><json:string>1000</json:string><json:string>2007</json:string><json:string>2010</json:string><json:string>1980</json:string></date><geogName></geogName><orgName><json:string>Idaho Technology Inc.</json:string><json:string>Roche Diagnostics, Mannheim, Germany</json:string><json:string>Life Technologies</json:string><json:string>Plant Biotechnology Journal</json:string><json:string>Blackwell Publishing Ltd</json:string><json:string>Illumina, Inc., San Diego</json:string><json:string>Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia Received</json:string><json:string>Biotium Inc</json:string><json:string>France Unite INRA</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>J.P. 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(2009)</json:string><json:string>Quarrie et al., 2005</json:string><json:string>Semagn et al., 2006</json:string><json:string>Mohring et al.</json:string><json:string>Koebner, 2004</json:string><json:string>Anderson et al., 1993</json:string><json:string>Visscher et al., 1996</json:string><json:string>Zavolan and Kepler, 2001</json:string><json:string>Reynolds et al., 2007</json:string><json:string>Gao et al.</json:string><json:string>Paux et al., 2008</json:string><json:string>Herrmann et al.</json:string><json:string>Clark et al., 2005</json:string><json:string>Kalendar and Schulman, 2006</json:string><json:string>Kim and Misra, 2007</json:string><json:string>16% of all crop area; FAOstat, 2008</json:string><json:string>Gupta et al., 1999</json:string><json:string>Ririe et al., 1997</json:string><json:string>Huang et al., 2002</json:string><json:string>Rabinowicz et al., 2005</json:string><json:string>McNeil et al. (2008)</json:string><json:string>Saintenac from the highly fragmented organization of repeats in the wheat genome as a result of deletion and nested insertions within TEs (Kronmiller and Wise, 2008</json:string><json:string>Lander et al., 1987</json:string><json:string>Etienne Paux et al.</json:string><json:string>Lin et al. (2009)</json:string><json:string>Kumar et al., 1997</json:string><json:string>Akhunov et al. (2009)</json:string><json:string>DeRose-Wilson and Gaut, 2007</json:string><json:string>Cone et al., 2002</json:string><json:string>Lukaszewski and Curtis, 1993</json:string><json:string>Paux 2005</json:string><json:string>Very et al., 2006</json:string><json:string>Somers et al., 2003</json:string><json:string>Paux et al., 2006</json:string><json:string>Plaschke et al., 1995</json:string><json:string>Bartos et al., 2008</json:string><json:string>Koebner and Summers, 2003</json:string><json:string>Endo and Gill, 1996</json:string><json:string>Akbari et al., 2006</json:string><json:string>Ganal et al., 2009</json:string><json:string>Kenward et al., 1999</json:string><json:string>Prasad et al., 2000</json:string><json:string>Pati et al., 2004</json:string><json:string>Varshney et al., 2007</json:string><json:string>Kruglyak and Nickerson, 2001</json:string><json:string>Li et al., 2004</json:string><json:string>Paux et al.</json:string><json:string>Schulman et al., 2004</json:string><json:string>FAOstat, 2008</json:string><json:string>Roy et al., 2006</json:string><json:string>Peleg et al., 2008</json:string><json:string>Wicker et al., 2002</json:string><json:string>Kalendar et al., 1999</json:string><json:string>Jeffery et al., 2007</json:string><json:string>Krypuy et al., 2006</json:string><json:string>Branco et al., 2007</json:string><json:string>Rutherford et al., 2000</json:string><json:string>Collins et al., 1998</json:string><json:string>Gill et al., 2004</json:string><json:string>Thuillet et al., 2004</json:string><json:string>Wittwer et al., 2003</json:string><json:string>Collard and Mackill, 2008</json:string><json:string>Sourdille et al., 2003</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-XCMZRD14-C</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - plant sciences</json:string><json:string>2 - biotechnology & applied microbiology</json:string></wos><scienceMetrix><json:string>1 - applied sciences</json:string><json:string>2 - enabling & strategic technologies</json:string><json:string>3 - biotechnology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Agricultural and Biological Sciences</json:string><json:string>3 - Plant Science</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Agricultural and Biological Sciences</json:string><json:string>3 - Agronomy and Crop Science</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biotechnology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>2010</publicationDate><copyrightDate>2010</copyrightDate><doi><json:string>10.1111/j.1467-7652.2009.00477.x</json:string></doi><id>5759BA874BA5C2AA9AD30274B75D8426174CF785</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/5759BA874BA5C2AA9AD30274B75D8426174CF785/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/5759BA874BA5C2AA9AD30274B75D8426174CF785/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/5759BA874BA5C2AA9AD30274B75D8426174CF785/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><licence>© 2010 INRA. Journal compilation © 2010 Blackwell Publishing Ltd</licence></availability><date type="published" when="2010-02"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat</title><title level="a" type="short">ISBPs: new markers for wheat breeding</title><author xml:id="author-0000" role="corresp"><persName><forename type="first">Etienne</forename><surname>Paux</surname></persName><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France<address><country key="FR"></country></address></affiliation><note type="foot">
These authors contributed equally to this work.</note><affiliation>* Correspondence (fax +33 473 62 44 53; e‐mail etienne.paux@clermont.inra.fr)</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Sébastien</forename><surname>Faure</surname></persName><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France<address><country key="FR"></country></address></affiliation><affiliation>Note: † † These authors contributed equally to this work.</affiliation><note type="foot">
Current address: Genetics & Genomics in Cereals, BIOGEMMA, 8 rue des Frères Lumière, 63028 Clermont Ferrand cedex 2, France.</note></author><author xml:id="author-0002"><persName><forename type="first">Frédéric</forename><surname>Choulet</surname></persName><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">Delphine</forename><surname>Roger</surname></persName><affiliation>Limagrain Verneuil Holding, Riom cedex, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Valérie</forename><surname>Gauthier</surname></persName><affiliation>Limagrain Verneuil Holding, Riom cedex, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0005"><persName><forename type="first">Jean‐Pierre</forename><surname>Martinant</surname></persName><affiliation>Limagrain Verneuil Holding, Riom cedex, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0006"><persName><forename type="first">Pierre</forename><surname>Sourdille</surname></persName><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0007"><persName><forename type="first">François</forename><surname>Balfourier</surname></persName><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0008"><persName><forename type="first">Marie‐Christine</forename><surname>Le Paslier</surname></persName><affiliation>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0009"><persName><forename type="first">Aurélie</forename><surname>Chauveau</surname></persName><affiliation>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0010"><persName><forename type="first">Mehmet</forename><surname>Cakir</surname></persName><affiliation>School of Biological Sciences & Biotechnology, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia<address><country key="AU"></country></address></affiliation></author><author xml:id="author-0011"><persName><forename type="first">Béatrice</forename><surname>Gandon</surname></persName><affiliation>Limagrain Verneuil Holding, Riom cedex, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0012"><persName><forename type="first">Catherine</forename><surname>Feuillet</surname></persName><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France<address><country key="FR"></country></address></affiliation></author><idno type="istex">5759BA874BA5C2AA9AD30274B75D8426174CF785</idno><idno type="ark">ark:/67375/WNG-XCMZRD14-C</idno><idno type="DOI">10.1111/j.1467-7652.2009.00477.x</idno><idno type="unit">PBI477</idno><idno type="toTypesetVersion">file:PBI.PBI477.pdf</idno></analytic><monogr><title level="j" type="main">Plant Biotechnology Journal</title><title level="j" type="alt">PLANT BIOTECHNOLOGY JOURNAL</title><idno type="pISSN">1467-7644</idno><idno type="eISSN">1467-7652</idno><idno type="book-DOI">10.1111/(ISSN)1467-7652</idno><idno type="book-part-DOI">10.1111/pbi.2010.8.issue-2</idno><idno type="product">PBI</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">8</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="196">196</biblScope><biblScope unit="page" to="210">210</biblScope><biblScope unit="page-count">15</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-02"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><head>Summary</head><p>In wheat, the deployment of marker‐assisted selection has long been hampered by the lack of markers compatible with high‐throughput cost‐effective genotyping techniques. Recently, insertion site‐based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high‐throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, <hi rend="italic">IsbpFinder,</hi> for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot<hi rend="superscript">®</hi> Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP‐derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost‐effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker‐assisted selection.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="k1">Insertion site‐based polymorphism</term><term xml:id="k2">molecular marker</term><term xml:id="k3">polymorphism</term><term xml:id="k4">mapping</term><term xml:id="k5">marker‐assisted selection</term><term xml:id="k6">wheat</term></keywords><keywords rend="tocHeading1"><term>Original Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5759BA874BA5C2AA9AD30274B75D8426174CF785/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1467-7652</doi><issn type="print">1467-7644</issn><issn type="electronic">1467-7652</issn><idGroup><id type="product" value="PBI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="PLANT BIOTECHNOLOGY JOURNAL">Plant Biotechnology Journal</title></titleGroup></publicationMeta><publicationMeta level="part" position="02002"><doi origin="wiley">10.1111/pbi.2010.8.issue-2</doi><numberingGroup><numbering type="journalVolume" number="8">8</numbering><numbering type="journalIssue" number="2">2</numbering></numberingGroup><coverDate startDate="2010-02">February 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="8" status="forIssue"><doi origin="wiley">10.1111/j.1467-7652.2009.00477.x</doi><idGroup><id type="unit" value="PBI477"></id></idGroup><countGroup><count type="pageTotal" number="15"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright>© 2010 INRA. 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<i>Correspondence</i> (fax +33 473 62 44 53; e‐mail <email>etienne.paux@clermont.inra.fr</email>)</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:PBI.PBI477.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 17 July 2009; revised 15 October 2009; accepted 15 October 2009.</unparsedEditorialHistory><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="0"></count></countGroup><titleGroup><title type="main">Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat</title><title type="shortAuthors"><i>Etienne Paux</i> et al.</title><title type="short">ISBPs: new markers for wheat breeding</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" noteRef="#fn1" corresponding="yes"><personName><givenNames>Etienne</givenNames><familyName>Paux</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" noteRef="#fn1 #fn2"><personName><givenNames>Sébastien</givenNames><familyName>Faure</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Frédéric</givenNames><familyName>Choulet</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>Delphine</givenNames><familyName>Roger</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>Valérie</givenNames><familyName>Gauthier</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a2"><personName><givenNames>Jean‐Pierre</givenNames><familyName>Martinant</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a1"><personName><givenNames>Pierre</givenNames><familyName>Sourdille</familyName></personName></creator><creator creatorRole="author" xml:id="cr8" affiliationRef="#a1"><personName><givenNames>François</givenNames><familyName>Balfourier</familyName></personName></creator><creator creatorRole="author" xml:id="cr9" affiliationRef="#a3"><personName><givenNames>Marie‐Christine</givenNames><familyName>Le Paslier</familyName></personName></creator><creator creatorRole="author" xml:id="cr10" affiliationRef="#a3"><personName><givenNames>Aurélie</givenNames><familyName>Chauveau</familyName></personName></creator><creator creatorRole="author" xml:id="cr11" affiliationRef="#a4"><personName><givenNames>Mehmet</givenNames><familyName>Cakir</familyName></personName></creator><creator creatorRole="author" xml:id="cr12" affiliationRef="#a2"><personName><givenNames>Béatrice</givenNames><familyName>Gandon</familyName></personName></creator><creator creatorRole="author" xml:id="cr13" affiliationRef="#a1"><personName><givenNames>Catherine</givenNames><familyName>Feuillet</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR"><unparsedAffiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>Limagrain Verneuil Holding, Riom cedex, France</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="FR"><unparsedAffiliation>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="AU"><unparsedAffiliation>School of Biological Sciences & Biotechnology, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">Insertion site‐based polymorphism</keyword><keyword xml:id="k2">molecular marker</keyword><keyword xml:id="k3">polymorphism</keyword><keyword xml:id="k4">mapping</keyword><keyword xml:id="k5">marker‐assisted selection</keyword><keyword xml:id="k6">wheat</keyword></keywordGroup><supportingInformation><p><b>Table S1</b> List of 157 sequenced ISBP markers, with sequence of origin and primers used for PCR amplification.</p><p><b>Table S2</b> List of 25 ISBPs and 53 ISBP‐derived SNPs genotyped on Illumina platform. Each marker is associated with the name of the corresponding ISBP, the type of polymorphism (presence/absence or SNP), the design score, the context sequence as well as the ASO A, ASO B and LSO primers.</p><p><b>Table S3</b> List of 46 European and 46 Australian wheat lines.</p><p>Please note: Wiley‐Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.</p><supportingInfoItem><mediaResource alt="supporting info item" href="urn-x:wiley:14677644:media:pbi477:PBI_477_sm_Supplementarytables"></mediaResource><caption>Supporting info item</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>In wheat, the deployment of marker‐assisted selection has long been hampered by the lack of markers compatible with high‐throughput cost‐effective genotyping techniques. Recently, insertion site‐based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high‐throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, <i>IsbpFinder,</i> for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot<sup>®</sup> Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP‐derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost‐effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker‐assisted selection.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><label>†</label><p>
These authors contributed equally to this work.</p></note><note xml:id="fn2"><label>‡</label><p>
Current address: Genetics & Genomics in Cereals, BIOGEMMA, 8 rue des Frères Lumière, 63028 Clermont Ferrand cedex 2, France.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>ISBPs: new markers for wheat breeding</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Insertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat</title></titleInfo><name type="personal"><namePart type="given">Etienne</namePart><namePart type="family">Paux</namePart><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</affiliation><description>These authors contributed equally to this work.</description><affiliation>E-mail: etienne.paux@clermont.inra.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sébastien</namePart><namePart type="family">Faure</namePart><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</affiliation><description>Note: These authors contributed equally to this work.</description><description>Current address: Genetics & Genomics in Cereals, BIOGEMMA, 8 rue des Frères Lumière, 63028 Clermont Ferrand cedex 2, France.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frédéric</namePart><namePart type="family">Choulet</namePart><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Delphine</namePart><namePart type="family">Roger</namePart><affiliation>Limagrain Verneuil Holding, Riom cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Valérie</namePart><namePart type="family">Gauthier</namePart><affiliation>Limagrain Verneuil Holding, Riom cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean‐Pierre</namePart><namePart type="family">Martinant</namePart><affiliation>Limagrain Verneuil Holding, Riom cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pierre</namePart><namePart type="family">Sourdille</namePart><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">François</namePart><namePart type="family">Balfourier</namePart><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marie‐Christine</namePart><namePart type="family">Le Paslier</namePart><affiliation>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Aurélie</namePart><namePart type="family">Chauveau</namePart><affiliation>Unité INRA‐EPGV UR1279, CEA, Institut de Génomique, Centre National de Génotypage, Evry cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mehmet</namePart><namePart type="family">Cakir</namePart><affiliation>School of Biological Sciences & Biotechnology, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Béatrice</namePart><namePart type="family">Gandon</namePart><affiliation>Limagrain Verneuil Holding, Riom cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Catherine</namePart><namePart type="family">Feuillet</namePart><affiliation>INRA UBP UMR 1095, Genetics, Diversity & Ecophysiology of Cereals, Clermont Ferrand, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-02</dateIssued><edition>Received 17 July 2009; revised 15 October 2009; accepted 15 October 2009.</edition><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">5</extent><extent unit="tables">0</extent></physicalDescription><abstract lang="en">In wheat, the deployment of marker‐assisted selection has long been hampered by the lack of markers compatible with high‐throughput cost‐effective genotyping techniques. Recently, insertion site‐based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high‐throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, IsbpFinder, for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot® Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP‐derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost‐effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker‐assisted selection.</abstract><subject lang="en"><genre>keywords</genre><topic>Insertion site‐based polymorphism</topic><topic>molecular marker</topic><topic>polymorphism</topic><topic>mapping</topic><topic>marker‐assisted selection</topic><topic>wheat</topic></subject><relatedItem type="host"><titleInfo><title>Plant Biotechnology Journal</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><note type="content"> Table S1 List of 157 sequenced ISBP markers, with sequence of origin and primers used for PCR amplification. Table S2 List of 25 ISBPs and 53 ISBP‐derived SNPs genotyped on Illumina platform. Each marker is associated with the name of the corresponding ISBP, the type of polymorphism (presence/absence or SNP), the design score, the context sequence as well as the ASO A, ASO B and LSO primers. Table S3 List of 46 European and 46 Australian wheat lines. Please note: Wiley‐Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Table S1 List of 157 sequenced ISBP markers, with sequence of origin and primers used for PCR amplification. Table S2 List of 25 ISBPs and 53 ISBP‐derived SNPs genotyped on Illumina platform. Each marker is associated with the name of the corresponding ISBP, the type of polymorphism (presence/absence or SNP), the design score, the context sequence as well as the ASO A, ASO B and LSO primers. Table S3 List of 46 European and 46 Australian wheat lines. Please note: Wiley‐Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Table S1 List of 157 sequenced ISBP markers, with sequence of origin and primers used for PCR amplification. Table S2 List of 25 ISBPs and 53 ISBP‐derived SNPs genotyped on Illumina platform. Each marker is associated with the name of the corresponding ISBP, the type of polymorphism (presence/absence or SNP), the design score, the context sequence as well as the ASO A, ASO B and LSO primers. Table S3 List of 46 European and 46 Australian wheat lines. Please note: Wiley‐Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Table S1 List of 157 sequenced ISBP markers, with sequence of origin and primers used for PCR amplification. Table S2 List of 25 ISBPs and 53 ISBP‐derived SNPs genotyped on Illumina platform. Each marker is associated with the name of the corresponding ISBP, the type of polymorphism (presence/absence or SNP), the design score, the context sequence as well as the ASO A, ASO B and LSO primers. Table S3 List of 46 European and 46 Australian wheat lines. Please note: Wiley‐Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.Supporting Info Item: Supporting info item - </note><identifier type="ISSN">1467-7644</identifier><identifier type="eISSN">1467-7652</identifier><identifier type="DOI">10.1111/(ISSN)1467-7652</identifier><identifier type="PublisherID">PBI</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>8</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>196</start><end>210</end><total>15</total></extent></part></relatedItem><identifier type="istex">5759BA874BA5C2AA9AD30274B75D8426174CF785</identifier><identifier type="ark">ark:/67375/WNG-XCMZRD14-C</identifier><identifier type="DOI">10.1111/j.1467-7652.2009.00477.x</identifier><identifier type="ArticleID">PBI477</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 INRA. 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