Systems-based analysis of the Sarcocystis neurona genome identifies pathways that contribute to a heteroxenous life cycle.
Identifieur interne : 000565 ( PubMed/Corpus ); précédent : 000564; suivant : 000566Systems-based analysis of the Sarcocystis neurona genome identifies pathways that contribute to a heteroxenous life cycle.
Auteurs : Tomasz Blazejewski ; Nirvana Nursimulu ; Viviana Pszenny ; Sriveny Dangoudoubiyam ; Sivaranjani Namasivayam ; Melissa A. Chiasson ; Kyle Chessman ; Michelle Tonkin ; Lakshmipuram S. Swapna ; Stacy S. Hung ; Joshua Bridgers ; Stacy M. Ricklefs ; Martin J. Boulanger ; Jitender P. Dubey ; Stephen F. Porcella ; Jessica C. Kissinger ; Daniel K. Howe ; Michael E. Grigg ; John ParkinsonSource :
- mBio [ 2150-7511 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Protozoan Proteins.
- classification : Sarcocystis.
- genetics : Sarcocystis.
- growth & development : Sarcocystis.
- metabolism : Sarcocystis.
- parasitology : Sarcocystosis.
- veterinary : Sarcocystosis.
- Animals, Genome, Protozoan, Humans, Life Cycle Stages, Phylogeny.
Abstract
Sarcocystis neurona is a member of the coccidia, a clade of single-celled parasites of medical and veterinary importance including Eimeria, Sarcocystis, Neospora, and Toxoplasma. Unlike Eimeria, a single-host enteric pathogen, Sarcocystis, Neospora, and Toxoplasma are two-host parasites that infect and produce infectious tissue cysts in a wide range of intermediate hosts. As a genus, Sarcocystis is one of the most successful protozoan parasites; all vertebrates, including birds, reptiles, fish, and mammals are hosts to at least one Sarcocystis species. Here we sequenced Sarcocystis neurona, the causal agent of fatal equine protozoal myeloencephalitis. The S. neurona genome is 127 Mbp, more than twice the size of other sequenced coccidian genomes. Comparative analyses identified conservation of the invasion machinery among the coccidia. However, many dense-granule and rhoptry kinase genes, responsible for altering host effector pathways in Toxoplasma and Neospora, are absent from S. neurona. Further, S. neurona has a divergent repertoire of SRS proteins, previously implicated in tissue cyst formation in Toxoplasma. Systems-based analyses identified a series of metabolic innovations, including the ability to exploit alternative sources of energy. Finally, we present an S. neurona model detailing conserved molecular innovations that promote the transition from a purely enteric lifestyle (Eimeria) to a heteroxenous parasite capable of infecting a wide range of intermediate hosts.
DOI: 10.1128/mBio.02445-14
PubMed: 25670772
Links to Exploration step
pubmed:25670772Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Systems-based analysis of the Sarcocystis neurona genome identifies pathways that contribute to a heteroxenous life cycle.</title><author><name sortKey="Blazejewski, Tomasz" sort="Blazejewski, Tomasz" uniqKey="Blazejewski T" first="Tomasz" last="Blazejewski">Tomasz Blazejewski</name><affiliation><nlm:affiliation>Program in Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Nursimulu, Nirvana" sort="Nursimulu, Nirvana" uniqKey="Nursimulu N" first="Nirvana" last="Nursimulu">Nirvana Nursimulu</name></author><author><name sortKey="Pszenny, Viviana" sort="Pszenny, Viviana" uniqKey="Pszenny V" first="Viviana" last="Pszenny">Viviana Pszenny</name><affiliation><nlm:affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Dangoudoubiyam, Sriveny" sort="Dangoudoubiyam, Sriveny" uniqKey="Dangoudoubiyam S" first="Sriveny" last="Dangoudoubiyam">Sriveny Dangoudoubiyam</name><affiliation><nlm:affiliation>Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Namasivayam, Sivaranjani" sort="Namasivayam, Sivaranjani" uniqKey="Namasivayam S" first="Sivaranjani" last="Namasivayam">Sivaranjani Namasivayam</name><affiliation><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Chiasson, Melissa A" sort="Chiasson, Melissa A" uniqKey="Chiasson M" first="Melissa A" last="Chiasson">Melissa A. Chiasson</name><affiliation><nlm:affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Chessman, Kyle" sort="Chessman, Kyle" uniqKey="Chessman K" first="Kyle" last="Chessman">Kyle Chessman</name></author><author><name sortKey="Tonkin, Michelle" sort="Tonkin, Michelle" uniqKey="Tonkin M" first="Michelle" last="Tonkin">Michelle Tonkin</name><affiliation><nlm:affiliation>Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Swapna, Lakshmipuram S" sort="Swapna, Lakshmipuram S" uniqKey="Swapna L" first="Lakshmipuram S" last="Swapna">Lakshmipuram S. Swapna</name><affiliation><nlm:affiliation>Program in Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Hung, Stacy S" sort="Hung, Stacy S" uniqKey="Hung S" first="Stacy S" last="Hung">Stacy S. Hung</name></author><author><name sortKey="Bridgers, Joshua" sort="Bridgers, Joshua" uniqKey="Bridgers J" first="Joshua" last="Bridgers">Joshua Bridgers</name><affiliation><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ricklefs, Stacy M" sort="Ricklefs, Stacy M" uniqKey="Ricklefs S" first="Stacy M" last="Ricklefs">Stacy M. Ricklefs</name><affiliation><nlm:affiliation>U.S. Department of Agriculture, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Boulanger, Martin J" sort="Boulanger, Martin J" uniqKey="Boulanger M" first="Martin J" last="Boulanger">Martin J. Boulanger</name><affiliation><nlm:affiliation>Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Dubey, Jitender P" sort="Dubey, Jitender P" uniqKey="Dubey J" first="Jitender P" last="Dubey">Jitender P. Dubey</name><affiliation><nlm:affiliation>U.S. Department of Agriculture, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Porcella, Stephen F" sort="Porcella, Stephen F" uniqKey="Porcella S" first="Stephen F" last="Porcella">Stephen F. Porcella</name><affiliation><nlm:affiliation>Genomics Unit, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kissinger, Jessica C" sort="Kissinger, Jessica C" uniqKey="Kissinger J" first="Jessica C" last="Kissinger">Jessica C. Kissinger</name></author><author><name sortKey="Howe, Daniel K" sort="Howe, Daniel K" uniqKey="Howe D" first="Daniel K" last="Howe">Daniel K. Howe</name><affiliation><nlm:affiliation>Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Grigg, Michael E" sort="Grigg, Michael E" uniqKey="Grigg M" first="Michael E" last="Grigg">Michael E. Grigg</name><affiliation><nlm:affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA john.parkinson@utoronto.ca griggm@niaid.nih.gov.</nlm:affiliation></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation><nlm:affiliation>john.parkinson@utoronto.ca griggm@niaid.nih.gov.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25670772</idno><idno type="pmid">25670772</idno><idno type="doi">10.1128/mBio.02445-14</idno><idno type="wicri:Area/PubMed/Corpus">000565</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000565</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Systems-based analysis of the Sarcocystis neurona genome identifies pathways that contribute to a heteroxenous life cycle.</title><author><name sortKey="Blazejewski, Tomasz" sort="Blazejewski, Tomasz" uniqKey="Blazejewski T" first="Tomasz" last="Blazejewski">Tomasz Blazejewski</name><affiliation><nlm:affiliation>Program in Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Nursimulu, Nirvana" sort="Nursimulu, Nirvana" uniqKey="Nursimulu N" first="Nirvana" last="Nursimulu">Nirvana Nursimulu</name></author><author><name sortKey="Pszenny, Viviana" sort="Pszenny, Viviana" uniqKey="Pszenny V" first="Viviana" last="Pszenny">Viviana Pszenny</name><affiliation><nlm:affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Dangoudoubiyam, Sriveny" sort="Dangoudoubiyam, Sriveny" uniqKey="Dangoudoubiyam S" first="Sriveny" last="Dangoudoubiyam">Sriveny Dangoudoubiyam</name><affiliation><nlm:affiliation>Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Namasivayam, Sivaranjani" sort="Namasivayam, Sivaranjani" uniqKey="Namasivayam S" first="Sivaranjani" last="Namasivayam">Sivaranjani Namasivayam</name><affiliation><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Chiasson, Melissa A" sort="Chiasson, Melissa A" uniqKey="Chiasson M" first="Melissa A" last="Chiasson">Melissa A. Chiasson</name><affiliation><nlm:affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Chessman, Kyle" sort="Chessman, Kyle" uniqKey="Chessman K" first="Kyle" last="Chessman">Kyle Chessman</name></author><author><name sortKey="Tonkin, Michelle" sort="Tonkin, Michelle" uniqKey="Tonkin M" first="Michelle" last="Tonkin">Michelle Tonkin</name><affiliation><nlm:affiliation>Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Swapna, Lakshmipuram S" sort="Swapna, Lakshmipuram S" uniqKey="Swapna L" first="Lakshmipuram S" last="Swapna">Lakshmipuram S. Swapna</name><affiliation><nlm:affiliation>Program in Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Hung, Stacy S" sort="Hung, Stacy S" uniqKey="Hung S" first="Stacy S" last="Hung">Stacy S. Hung</name></author><author><name sortKey="Bridgers, Joshua" sort="Bridgers, Joshua" uniqKey="Bridgers J" first="Joshua" last="Bridgers">Joshua Bridgers</name><affiliation><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ricklefs, Stacy M" sort="Ricklefs, Stacy M" uniqKey="Ricklefs S" first="Stacy M" last="Ricklefs">Stacy M. Ricklefs</name><affiliation><nlm:affiliation>U.S. Department of Agriculture, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Boulanger, Martin J" sort="Boulanger, Martin J" uniqKey="Boulanger M" first="Martin J" last="Boulanger">Martin J. Boulanger</name><affiliation><nlm:affiliation>Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Dubey, Jitender P" sort="Dubey, Jitender P" uniqKey="Dubey J" first="Jitender P" last="Dubey">Jitender P. Dubey</name><affiliation><nlm:affiliation>U.S. Department of Agriculture, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, Maryland, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Porcella, Stephen F" sort="Porcella, Stephen F" uniqKey="Porcella S" first="Stephen F" last="Porcella">Stephen F. Porcella</name><affiliation><nlm:affiliation>Genomics Unit, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kissinger, Jessica C" sort="Kissinger, Jessica C" uniqKey="Kissinger J" first="Jessica C" last="Kissinger">Jessica C. Kissinger</name></author><author><name sortKey="Howe, Daniel K" sort="Howe, Daniel K" uniqKey="Howe D" first="Daniel K" last="Howe">Daniel K. Howe</name><affiliation><nlm:affiliation>Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Grigg, Michael E" sort="Grigg, Michael E" uniqKey="Grigg M" first="Michael E" last="Grigg">Michael E. Grigg</name><affiliation><nlm:affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA john.parkinson@utoronto.ca griggm@niaid.nih.gov.</nlm:affiliation></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation><nlm:affiliation>john.parkinson@utoronto.ca griggm@niaid.nih.gov.</nlm:affiliation></affiliation></author></analytic><series><title level="j">mBio</title><idno type="eISSN">2150-7511</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Genome, Protozoan</term><term>Humans</term><term>Life Cycle Stages</term><term>Phylogeny</term><term>Protozoan Proteins (genetics)</term><term>Sarcocystis (classification)</term><term>Sarcocystis (genetics)</term><term>Sarcocystis (growth & development)</term><term>Sarcocystis (metabolism)</term><term>Sarcocystosis (parasitology)</term><term>Sarcocystosis (veterinary)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Sarcocystis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Sarcocystis</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Sarcocystis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Sarcocystis</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Sarcocystosis</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Sarcocystosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genome, Protozoan</term><term>Humans</term><term>Life Cycle Stages</term><term>Phylogeny</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sarcocystis neurona is a member of the coccidia, a clade of single-celled parasites of medical and veterinary importance including Eimeria, Sarcocystis, Neospora, and Toxoplasma. Unlike Eimeria, a single-host enteric pathogen, Sarcocystis, Neospora, and Toxoplasma are two-host parasites that infect and produce infectious tissue cysts in a wide range of intermediate hosts. As a genus, Sarcocystis is one of the most successful protozoan parasites; all vertebrates, including birds, reptiles, fish, and mammals are hosts to at least one Sarcocystis species. Here we sequenced Sarcocystis neurona, the causal agent of fatal equine protozoal myeloencephalitis. The S. neurona genome is 127 Mbp, more than twice the size of other sequenced coccidian genomes. Comparative analyses identified conservation of the invasion machinery among the coccidia. However, many dense-granule and rhoptry kinase genes, responsible for altering host effector pathways in Toxoplasma and Neospora, are absent from S. neurona. Further, S. neurona has a divergent repertoire of SRS proteins, previously implicated in tissue cyst formation in Toxoplasma. Systems-based analyses identified a series of metabolic innovations, including the ability to exploit alternative sources of energy. Finally, we present an S. neurona model detailing conserved molecular innovations that promote the transition from a purely enteric lifestyle (Eimeria) to a heteroxenous parasite capable of infecting a wide range of intermediate hosts.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25670772</PMID><DateCreated><Year>2015</Year><Month>02</Month><Day>11</Day></DateCreated><DateCompleted><Year>2016</Year><Month>03</Month><Day>14</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>19</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2150-7511</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Feb</Month><Day>10</Day></PubDate></JournalIssue><Title>mBio</Title><ISOAbbreviation>MBio</ISOAbbreviation></Journal><ArticleTitle>Systems-based analysis of the Sarcocystis neurona genome identifies pathways that contribute to a heteroxenous life cycle.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1128/mBio.02445-14</ELocationID><ELocationID EIdType="pii" ValidYN="Y">e02445-14</ELocationID><Abstract><AbstractText Label="UNLABELLED">Sarcocystis neurona is a member of the coccidia, a clade of single-celled parasites of medical and veterinary importance including Eimeria, Sarcocystis, Neospora, and Toxoplasma. Unlike Eimeria, a single-host enteric pathogen, Sarcocystis, Neospora, and Toxoplasma are two-host parasites that infect and produce infectious tissue cysts in a wide range of intermediate hosts. As a genus, Sarcocystis is one of the most successful protozoan parasites; all vertebrates, including birds, reptiles, fish, and mammals are hosts to at least one Sarcocystis species. Here we sequenced Sarcocystis neurona, the causal agent of fatal equine protozoal myeloencephalitis. The S. neurona genome is 127 Mbp, more than twice the size of other sequenced coccidian genomes. Comparative analyses identified conservation of the invasion machinery among the coccidia. However, many dense-granule and rhoptry kinase genes, responsible for altering host effector pathways in Toxoplasma and Neospora, are absent from S. neurona. Further, S. neurona has a divergent repertoire of SRS proteins, previously implicated in tissue cyst formation in Toxoplasma. Systems-based analyses identified a series of metabolic innovations, including the ability to exploit alternative sources of energy. Finally, we present an S. neurona model detailing conserved molecular innovations that promote the transition from a purely enteric lifestyle (Eimeria) to a heteroxenous parasite capable of infecting a wide range of intermediate hosts.</AbstractText><AbstractText Label="IMPORTANCE" NlmCategory="OBJECTIVE">Sarcocystis neurona is a member of the coccidia, a clade of single-celled apicomplexan parasites responsible for major economic and health care burdens worldwide. A cousin of Plasmodium, Cryptosporidium, Theileria, and Eimeria, Sarcocystis is one of the most successful parasite genera; it is capable of infecting all vertebrates (fish, reptiles, birds, and mammals-including humans). The past decade has witnessed an increasing number of human outbreaks of clinical significance associated with acute sarcocystosis. Among Sarcocystis species, S. neurona has a wide host range and causes fatal encephalitis in horses, marine mammals, and several other mammals. To provide insights into the transition from a purely enteric parasite (e.g., Eimeria) to one that forms tissue cysts (Toxoplasma), we present the first genome sequence of S. neurona. Comparisons with other coccidian genomes highlight the molecular innovations that drive its distinct life cycle strategies.</AbstractText><CopyrightInformation>Copyright © 2015 Blazejewski et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Blazejewski</LastName><ForeName>Tomasz</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Program in Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nursimulu</LastName><ForeName>Nirvana</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Pszenny</LastName><ForeName>Viviana</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dangoudoubiyam</LastName><ForeName>Sriveny</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Namasivayam</LastName><ForeName>Sivaranjani</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Genetics, University of Georgia, Athens, Georgia, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chiasson</LastName><ForeName>Melissa A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chessman</LastName><ForeName>Kyle</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Tonkin</LastName><ForeName>Michelle</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Swapna</LastName><ForeName>Lakshmipuram S</ForeName><Initials>LS</Initials><AffiliationInfo><Affiliation>Program in Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hung</LastName><ForeName>Stacy S</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Bridgers</LastName><ForeName>Joshua</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Genetics, University of Georgia, Athens, Georgia, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ricklefs</LastName><ForeName>Stacy M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>U.S. Department of Agriculture, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boulanger</LastName><ForeName>Martin J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dubey</LastName><ForeName>Jitender P</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>U.S. Department of Agriculture, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Porcella</LastName><ForeName>Stephen F</ForeName><Initials>SF</Initials><AffiliationInfo><Affiliation>Genomics Unit, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kissinger</LastName><ForeName>Jessica C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Howe</LastName><ForeName>Daniel K</ForeName><Initials>DK</Initials><AffiliationInfo><Affiliation>Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grigg</LastName><ForeName>Michael E</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA john.parkinson@utoronto.ca griggm@niaid.nih.gov.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parkinson</LastName><ForeName>John</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>john.parkinson@utoronto.ca griggm@niaid.nih.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>PDB</DataBankName><AccessionNumberList><AccessionNumber>2x2z</AccessionNumber><AccessionNumber>2y8t</AccessionNumber></AccessionNumberList></DataBank></DataBankList><GrantList CompleteYN="Y"><Grant><GrantID>MOP 84556</GrantID><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant><Grant><Agency>Intramural NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>02</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>MBio</MedlineTA><NlmUniqueID>101519231</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015800">Protozoan Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Parasitol. 2000 Dec;86(6):1276-80</RefSource><PMID Version="1">11191904</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Parasitol. 1995 Dec;81(6):916-9</RefSource><PMID Version="1">8544064</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vet Parasitol. 2001 Feb 26;95(2-4):89-131</RefSource><PMID 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Feb 5;152(3):563-78</RefSource><PMID Version="1">11157983</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018503" MajorTopicYN="Y">Genome, Protozoan</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008018" MajorTopicYN="N">Life Cycle Stages</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015800" MajorTopicYN="N">Protozoan Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012522" MajorTopicYN="N">Sarcocystis</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012523" MajorTopicYN="N">Sarcocystosis</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4337577</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>2</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>2</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25670772</ArticleId><ArticleId IdType="pii">mBio.02445-14</ArticleId><ArticleId IdType="doi">10.1128/mBio.02445-14</ArticleId><ArticleId IdType="pmc">PMC4337577</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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