Granzyme B mediates both direct and indirect cleavage of extracellular matrix in skin after chronic low-dose ultraviolet light irradiation.
Identifieur interne : 000566 ( PubMed/Corpus ); précédent : 000565; suivant : 000567Granzyme B mediates both direct and indirect cleavage of extracellular matrix in skin after chronic low-dose ultraviolet light irradiation.
Auteurs : Leigh G. Parkinson ; Ana Toro ; Hongyan Zhao ; Keddie Brown ; Scott J. Tebbutt ; David J. GranvilleSource :
- Aging cell [ 1474-9726 ] ; 2015.
English descriptors
- KwdEn :
- Animals, Cell Count, Collagen (metabolism), Decorin (metabolism), Dermis (pathology), Dermis (radiation effects), Dose-Response Relationship, Radiation, Extracellular Matrix (metabolism), Female, Fibroblasts (enzymology), Fibroblasts (radiation effects), Fibronectins (metabolism), Granzymes (deficiency), Granzymes (metabolism), Mast Cells (enzymology), Mast Cells (radiation effects), Matrix Metalloproteinase 1 (metabolism), Mice, Inbred C57BL, Mice, Knockout, Models, Biological, Protein Transport (radiation effects), Proteolysis (radiation effects), Skin (metabolism), Skin (radiation effects), Skin Aging (radiation effects), Ultraviolet Rays.
- MESH :
- chemical , deficiency : Granzymes.
- chemical , metabolism : Collagen, Decorin, Fibronectins, Granzymes, Matrix Metalloproteinase 1.
- enzymology : Fibroblasts, Mast Cells.
- metabolism : Extracellular Matrix, Skin.
- pathology : Dermis.
- radiation effects : Dermis, Fibroblasts, Mast Cells, Protein Transport, Proteolysis, Skin, Skin Aging.
- Animals, Cell Count, Dose-Response Relationship, Radiation, Female, Mice, Inbred C57BL, Mice, Knockout, Models, Biological, Ultraviolet Rays.
Abstract
Extracellular matrix (ECM) degradation is a hallmark of many chronic inflammatory diseases that can lead to a loss of function, aging, and disease progression. Ultraviolet light (UV) irradiation from the sun is widely considered as the major cause of visible human skin aging, causing increased inflammation and enhanced ECM degradation. Granzyme B (GzmB), a serine protease that is expressed by a variety of cells, accumulates in the extracellular milieu during chronic inflammation and cleaves a number of ECM proteins. We hypothesized that GzmB contributes to ECM degradation in the skin after UV irradiation through both direct cleavage of ECM proteins and indirectly through the induction of other proteinases. Wild-type and GzmB-knockout mice were repeatedly exposed to minimal erythemal doses of solar-simulated UV irradiation for 20 weeks. GzmB expression was significantly increased in wild-type treated skin compared to nonirradiated controls, colocalizing to keratinocytes and to an increased mast cell population. GzmB deficiency significantly protected against the formation of wrinkles and the loss of dermal collagen density, which was related to the cleavage of decorin, an abundant proteoglycan involved in collagen fibrillogenesis and integrity. GzmB also cleaved fibronectin, and GzmB-mediated fibronectin fragments increased the expression of collagen-degrading matrix metalloproteinase-1 (MMP-1) in fibroblasts. Collectively, these findings indicate a significant role for GzmB in ECM degradation that may have implications in many age-related chronic inflammatory diseases.
DOI: 10.1111/acel.12298
PubMed: 25495009
Links to Exploration step
pubmed:25495009Le document en format XML
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Parkinson</name><affiliation><nlm:affiliation>Centre for Heart Lung Innovation, St. Paul's Hospital, Vancouver, British Columbia, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Toro, Ana" sort="Toro, Ana" uniqKey="Toro A" first="Ana" last="Toro">Ana Toro</name></author><author><name sortKey="Zhao, Hongyan" sort="Zhao, Hongyan" uniqKey="Zhao H" first="Hongyan" last="Zhao">Hongyan Zhao</name></author><author><name sortKey="Brown, Keddie" sort="Brown, Keddie" uniqKey="Brown K" first="Keddie" last="Brown">Keddie Brown</name></author><author><name sortKey="Tebbutt, Scott J" sort="Tebbutt, Scott J" uniqKey="Tebbutt S" first="Scott J" last="Tebbutt">Scott J. Tebbutt</name></author><author><name sortKey="Granville, David J" sort="Granville, David J" uniqKey="Granville D" first="David J" last="Granville">David J. Granville</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25495009</idno><idno type="pmid">25495009</idno><idno type="doi">10.1111/acel.12298</idno><idno type="wicri:Area/PubMed/Corpus">000566</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000566</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Granzyme B mediates both direct and indirect cleavage of extracellular matrix in skin after chronic low-dose ultraviolet light irradiation.</title><author><name sortKey="Parkinson, Leigh G" sort="Parkinson, Leigh G" uniqKey="Parkinson L" first="Leigh G" last="Parkinson">Leigh G. Parkinson</name><affiliation><nlm:affiliation>Centre for Heart Lung Innovation, St. Paul's Hospital, Vancouver, British Columbia, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Toro, Ana" sort="Toro, Ana" uniqKey="Toro A" first="Ana" last="Toro">Ana Toro</name></author><author><name sortKey="Zhao, Hongyan" sort="Zhao, Hongyan" uniqKey="Zhao H" first="Hongyan" last="Zhao">Hongyan Zhao</name></author><author><name sortKey="Brown, Keddie" sort="Brown, Keddie" uniqKey="Brown K" first="Keddie" last="Brown">Keddie Brown</name></author><author><name sortKey="Tebbutt, Scott J" sort="Tebbutt, Scott J" uniqKey="Tebbutt S" first="Scott J" last="Tebbutt">Scott J. Tebbutt</name></author><author><name sortKey="Granville, David J" sort="Granville, David J" uniqKey="Granville D" first="David J" last="Granville">David J. Granville</name></author></analytic><series><title level="j">Aging cell</title><idno type="eISSN">1474-9726</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Count</term><term>Collagen (metabolism)</term><term>Decorin (metabolism)</term><term>Dermis (pathology)</term><term>Dermis (radiation effects)</term><term>Dose-Response Relationship, Radiation</term><term>Extracellular Matrix (metabolism)</term><term>Female</term><term>Fibroblasts (enzymology)</term><term>Fibroblasts (radiation effects)</term><term>Fibronectins (metabolism)</term><term>Granzymes (deficiency)</term><term>Granzymes (metabolism)</term><term>Mast Cells (enzymology)</term><term>Mast Cells (radiation effects)</term><term>Matrix Metalloproteinase 1 (metabolism)</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Models, Biological</term><term>Protein Transport (radiation effects)</term><term>Proteolysis (radiation effects)</term><term>Skin (metabolism)</term><term>Skin (radiation effects)</term><term>Skin Aging (radiation effects)</term><term>Ultraviolet Rays</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Granzymes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Collagen</term><term>Decorin</term><term>Fibronectins</term><term>Granzymes</term><term>Matrix Metalloproteinase 1</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Fibroblasts</term><term>Mast Cells</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Extracellular Matrix</term><term>Skin</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Dermis</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Dermis</term><term>Fibroblasts</term><term>Mast Cells</term><term>Protein Transport</term><term>Proteolysis</term><term>Skin</term><term>Skin Aging</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Count</term><term>Dose-Response Relationship, Radiation</term><term>Female</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Models, Biological</term><term>Ultraviolet Rays</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Extracellular matrix (ECM) degradation is a hallmark of many chronic inflammatory diseases that can lead to a loss of function, aging, and disease progression. Ultraviolet light (UV) irradiation from the sun is widely considered as the major cause of visible human skin aging, causing increased inflammation and enhanced ECM degradation. Granzyme B (GzmB), a serine protease that is expressed by a variety of cells, accumulates in the extracellular milieu during chronic inflammation and cleaves a number of ECM proteins. We hypothesized that GzmB contributes to ECM degradation in the skin after UV irradiation through both direct cleavage of ECM proteins and indirectly through the induction of other proteinases. Wild-type and GzmB-knockout mice were repeatedly exposed to minimal erythemal doses of solar-simulated UV irradiation for 20 weeks. GzmB expression was significantly increased in wild-type treated skin compared to nonirradiated controls, colocalizing to keratinocytes and to an increased mast cell population. GzmB deficiency significantly protected against the formation of wrinkles and the loss of dermal collagen density, which was related to the cleavage of decorin, an abundant proteoglycan involved in collagen fibrillogenesis and integrity. GzmB also cleaved fibronectin, and GzmB-mediated fibronectin fragments increased the expression of collagen-degrading matrix metalloproteinase-1 (MMP-1) in fibroblasts. Collectively, these findings indicate a significant role for GzmB in ECM degradation that may have implications in many age-related chronic inflammatory diseases.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25495009</PMID><DateCreated><Year>2015</Year><Month>01</Month><Day>28</Day></DateCreated><DateCompleted><Year>2015</Year><Month>09</Month><Day>28</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1474-9726</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Aging cell</Title><ISOAbbreviation>Aging Cell</ISOAbbreviation></Journal><ArticleTitle>Granzyme B mediates both direct and indirect cleavage of extracellular matrix in skin after chronic low-dose ultraviolet light irradiation.</ArticleTitle><Pagination><MedlinePgn>67-77</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/acel.12298</ELocationID><Abstract><AbstractText>Extracellular matrix (ECM) degradation is a hallmark of many chronic inflammatory diseases that can lead to a loss of function, aging, and disease progression. Ultraviolet light (UV) irradiation from the sun is widely considered as the major cause of visible human skin aging, causing increased inflammation and enhanced ECM degradation. Granzyme B (GzmB), a serine protease that is expressed by a variety of cells, accumulates in the extracellular milieu during chronic inflammation and cleaves a number of ECM proteins. We hypothesized that GzmB contributes to ECM degradation in the skin after UV irradiation through both direct cleavage of ECM proteins and indirectly through the induction of other proteinases. Wild-type and GzmB-knockout mice were repeatedly exposed to minimal erythemal doses of solar-simulated UV irradiation for 20 weeks. GzmB expression was significantly increased in wild-type treated skin compared to nonirradiated controls, colocalizing to keratinocytes and to an increased mast cell population. GzmB deficiency significantly protected against the formation of wrinkles and the loss of dermal collagen density, which was related to the cleavage of decorin, an abundant proteoglycan involved in collagen fibrillogenesis and integrity. GzmB also cleaved fibronectin, and GzmB-mediated fibronectin fragments increased the expression of collagen-degrading matrix metalloproteinase-1 (MMP-1) in fibroblasts. Collectively, these findings indicate a significant role for GzmB in ECM degradation that may have implications in many age-related chronic inflammatory diseases.</AbstractText><CopyrightInformation>© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Parkinson</LastName><ForeName>Leigh G</ForeName><Initials>LG</Initials><AffiliationInfo><Affiliation>Centre for Heart Lung Innovation, St. Paul's Hospital, Vancouver, British Columbia, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Toro</LastName><ForeName>Ana</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Hongyan</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Brown</LastName><ForeName>Keddie</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Tebbutt</LastName><ForeName>Scott J</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Granville</LastName><ForeName>David J</ForeName><Initials>DJ</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>12</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Aging Cell</MedlineTA><NlmUniqueID>101130839</NlmUniqueID><ISSNLinking>1474-9718</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D058575">Decorin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005353">Fibronectins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-34-5</RegistryNumber><NameOfSubstance UI="D003094">Collagen</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.-</RegistryNumber><NameOfSubstance UI="D053804">Granzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.24.7</RegistryNumber><NameOfSubstance UI="D020781">Matrix Metalloproteinase 1</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Immunol Rev. 1988 Mar;103:53-71</RefSource><PMID Version="1">3292396</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Br J Dermatol. 1992 Sep;127 Suppl 41:21-4</RefSource><PMID Version="1">1327061</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Dent Res. 2011 Jul;90(7):830-40</RefSource><PMID Version="1">21447699</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2005 Jun 24;280(25):23549-58</RefSource><PMID 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Jan;174(1):101-14</RefSource><PMID Version="1">19116368</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002452" MajorTopicYN="N">Cell Count</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003094" MajorTopicYN="N">Collagen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058575" MajorTopicYN="N">Decorin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020405" MajorTopicYN="N">Dermis</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004307" MajorTopicYN="N">Dose-Response Relationship, Radiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005109" MajorTopicYN="N">Extracellular Matrix</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005347" MajorTopicYN="N">Fibroblasts</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005353" MajorTopicYN="N">Fibronectins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053804" MajorTopicYN="N">Granzymes</DescriptorName><QualifierName UI="Q000172" MajorTopicYN="N">deficiency</QualifierName><QualifierName UI="Q000378" 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effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059748" MajorTopicYN="N">Proteolysis</DescriptorName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012867" MajorTopicYN="N">Skin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015595" MajorTopicYN="N">Skin Aging</DescriptorName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014466" MajorTopicYN="Y">Ultraviolet Rays</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4326907</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">aging</Keyword><Keyword MajorTopicYN="N">collagen</Keyword><Keyword MajorTopicYN="N">extracellular matrix</Keyword><Keyword MajorTopicYN="N">fibronectin</Keyword><Keyword MajorTopicYN="N">granzyme B</Keyword><Keyword MajorTopicYN="N">matrix metalloproteinase</Keyword><Keyword MajorTopicYN="N">photoaging</Keyword><Keyword MajorTopicYN="N">skin</Keyword><Keyword MajorTopicYN="N">ultraviolet light</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>11</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25495009</ArticleId><ArticleId 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