Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants.
Identifieur interne : 000E19 ( PubMed/Checkpoint ); précédent : 000E18; suivant : 000E20Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants.
Auteurs : Welington L. Araújo [Brésil] ; Joelma Marcon ; Walter Maccheroni ; Jan Dirk Van Elsas ; Jim W L. Van Vuurde ; João Lúcio AzevedoSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 2002.
English descriptors
- KwdEn :
- Bacillus cereus (genetics), Bacillus cereus (metabolism), Bacterial Typing Techniques, Citrus (microbiology), DNA, Bacterial (analysis), Drug Resistance, Bacterial, Electrophoresis, Polyacrylamide Gel, Gammaproteobacteria (classification), Gammaproteobacteria (genetics), Plant Diseases (microbiology), Plant Leaves (microbiology), RNA, Ribosomal, 16S (genetics).
- MESH :
- chemical , analysis : DNA, Bacterial.
- classification : Gammaproteobacteria.
- genetics : Bacillus cereus, Gammaproteobacteria, RNA, Ribosomal, 16S.
- metabolism : Bacillus cereus.
- microbiology : Citrus, Plant Diseases, Plant Leaves.
- Bacterial Typing Techniques, Drug Resistance, Bacterial, Electrophoresis, Polyacrylamide Gel.
Abstract
Citrus variegated chlorosis (CVC) is caused by Xylella fastidiosa, a phytopathogenic bacterium that can infect all Citrus sinensis cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. The endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (DGGE). Dominant isolates were characterized by fatty-acid methyl ester analysis as Bacillus pumilus, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium spp. (including Methylobacterium extorquens, M. fujisawaense, M. mesophilicum, M. radiotolerans, and M. zatmanii), Nocardia sp., Pantoea agglomerans, and Xanthomonas campestris. We observed a relationship between CVC symptoms and the frequency of isolation of species of Methylobacterium, the genus that we most frequently isolated from symptomatic plants. In contrast, we isolated C. flaccumfaciens significantly more frequently from asymptomatic plants than from those with symptoms of CVC while P. agglomerans was frequently isolated from tangerine (Citrus reticulata) and sweet-orange (C. sinensis) plants, irrespective of whether the plants were symptomatic or asymptomatic or showed symptoms of CVC. DGGE analysis of 16S rRNA gene fragments amplified from total plant DNA resulted in several bands that matched those from the bacterial isolates, indicating that DGGE profiles can be used to detect some endophytic bacteria of citrus plants. However, some bands had no match with any isolate, suggesting the occurrence of other, nonculturable or as yet uncultured, endophytic bacteria. A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of C. flaccumfaciens in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC.
PubMed: 12324338
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Araújo</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil. wlaraujo@esalq.usp.br</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Marcon, Joelma" sort="Marcon, Joelma" uniqKey="Marcon J" first="Joelma" last="Marcon">Joelma Marcon</name></author><author><name sortKey="Maccheroni, Walter" sort="Maccheroni, Walter" uniqKey="Maccheroni W" first="Walter" last="Maccheroni">Walter Maccheroni</name></author><author><name sortKey="Van Elsas, Jan Dirk" sort="Van Elsas, Jan Dirk" uniqKey="Van Elsas J" first="Jan Dirk" last="Van Elsas">Jan Dirk Van Elsas</name></author><author><name sortKey="Van Vuurde, Jim W L" sort="Van Vuurde, Jim W L" uniqKey="Van Vuurde J" first="Jim W L" last="Van Vuurde">Jim W L. Van Vuurde</name></author><author><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12324338</idno><idno type="pmid">12324338</idno><idno type="wicri:Area/PubMed/Corpus">000E72</idno><idno type="wicri:Area/PubMed/Curation">000E72</idno><idno type="wicri:Area/PubMed/Checkpoint">000E72</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants.</title><author><name sortKey="Araujo, Welington L" sort="Araujo, Welington L" uniqKey="Araujo W" first="Welington L" last="Araújo">Welington L. Araújo</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil. wlaraujo@esalq.usp.br</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Marcon, Joelma" sort="Marcon, Joelma" uniqKey="Marcon J" first="Joelma" last="Marcon">Joelma Marcon</name></author><author><name sortKey="Maccheroni, Walter" sort="Maccheroni, Walter" uniqKey="Maccheroni W" first="Walter" last="Maccheroni">Walter Maccheroni</name></author><author><name sortKey="Van Elsas, Jan Dirk" sort="Van Elsas, Jan Dirk" uniqKey="Van Elsas J" first="Jan Dirk" last="Van Elsas">Jan Dirk Van Elsas</name></author><author><name sortKey="Van Vuurde, Jim W L" sort="Van Vuurde, Jim W L" uniqKey="Van Vuurde J" first="Jim W L" last="Van Vuurde">Jim W L. Van Vuurde</name></author><author><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacillus cereus (genetics)</term><term>Bacillus cereus (metabolism)</term><term>Bacterial Typing Techniques</term><term>Citrus (microbiology)</term><term>DNA, Bacterial (analysis)</term><term>Drug Resistance, Bacterial</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Gammaproteobacteria (classification)</term><term>Gammaproteobacteria (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>RNA, Ribosomal, 16S (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus cereus</term><term>Gammaproteobacteria</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus cereus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Drug Resistance, Bacterial</term><term>Electrophoresis, Polyacrylamide Gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus variegated chlorosis (CVC) is caused by Xylella fastidiosa, a phytopathogenic bacterium that can infect all Citrus sinensis cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. The endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (DGGE). Dominant isolates were characterized by fatty-acid methyl ester analysis as Bacillus pumilus, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium spp. (including Methylobacterium extorquens, M. fujisawaense, M. mesophilicum, M. radiotolerans, and M. zatmanii), Nocardia sp., Pantoea agglomerans, and Xanthomonas campestris. We observed a relationship between CVC symptoms and the frequency of isolation of species of Methylobacterium, the genus that we most frequently isolated from symptomatic plants. In contrast, we isolated C. flaccumfaciens significantly more frequently from asymptomatic plants than from those with symptoms of CVC while P. agglomerans was frequently isolated from tangerine (Citrus reticulata) and sweet-orange (C. sinensis) plants, irrespective of whether the plants were symptomatic or asymptomatic or showed symptoms of CVC. DGGE analysis of 16S rRNA gene fragments amplified from total plant DNA resulted in several bands that matched those from the bacterial isolates, indicating that DGGE profiles can be used to detect some endophytic bacteria of citrus plants. However, some bands had no match with any isolate, suggesting the occurrence of other, nonculturable or as yet uncultured, endophytic bacteria. A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of C. flaccumfaciens in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12324338</PMID><DateCreated><Year>2002</Year><Month>9</Month><Day>26</Day></DateCreated><DateCompleted><Year>2003</Year><Month>02</Month><Day>06</Day></DateCompleted><DateRevised><Year>2014</Year><Month>6</Month><Day>11</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>68</Volume><Issue>10</Issue><PubDate><Year>2002</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl. Environ. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants.</ArticleTitle><Pagination><MedlinePgn>4906-14</MedlinePgn></Pagination><Abstract><AbstractText>Citrus variegated chlorosis (CVC) is caused by Xylella fastidiosa, a phytopathogenic bacterium that can infect all Citrus sinensis cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. The endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (DGGE). Dominant isolates were characterized by fatty-acid methyl ester analysis as Bacillus pumilus, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium spp. (including Methylobacterium extorquens, M. fujisawaense, M. mesophilicum, M. radiotolerans, and M. zatmanii), Nocardia sp., Pantoea agglomerans, and Xanthomonas campestris. We observed a relationship between CVC symptoms and the frequency of isolation of species of Methylobacterium, the genus that we most frequently isolated from symptomatic plants. In contrast, we isolated C. flaccumfaciens significantly more frequently from asymptomatic plants than from those with symptoms of CVC while P. agglomerans was frequently isolated from tangerine (Citrus reticulata) and sweet-orange (C. sinensis) plants, irrespective of whether the plants were symptomatic or asymptomatic or showed symptoms of CVC. DGGE analysis of 16S rRNA gene fragments amplified from total plant DNA resulted in several bands that matched those from the bacterial isolates, indicating that DGGE profiles can be used to detect some endophytic bacteria of citrus plants. However, some bands had no match with any isolate, suggesting the occurrence of other, nonculturable or as yet uncultured, endophytic bacteria. A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of C. flaccumfaciens in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Araújo</LastName><ForeName>Welington L</ForeName><Initials>WL</Initials><AffiliationInfo><Affiliation>Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil. wlaraujo@esalq.usp.br</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marcon</LastName><ForeName>Joelma</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Maccheroni</LastName><ForeName>Walter</ForeName><Initials>W</Initials><Suffix>Jr</Suffix></Author><Author ValidYN="Y"><LastName>Van Elsas</LastName><ForeName>Jan Dirk</ForeName><Initials>JD</Initials></Author><Author ValidYN="Y"><LastName>Van Vuurde</LastName><ForeName>Jim W L</ForeName><Initials>JW</Initials></Author><Author ValidYN="Y"><LastName>Azevedo</LastName><ForeName>João Lúcio</ForeName><Initials>JL</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Appl Environ Microbiol. 2000 Jul;66(7):3073-7</RefSource><PMID Version="1">10877808</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 2000 Jul 13;406(6792):151-9</RefSource><PMID Version="1">10910347</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Curr Opin Microbiol. 2000 Oct;3(5):459-62</RefSource><PMID Version="1">11050442</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Appl Environ Microbiol. 1997 Sep;63(9):3614-21</RefSource><PMID Version="1">9293013</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Can J Microbiol. 2001 Mar;47(3):229-36</RefSource><PMID Version="1">11315114</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Appl Environ Microbiol. 1993 Mar;59(3):695-700</RefSource><PMID Version="1">7683183</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Curr Microbiol. 1995 Dec;31(6):377-81</RefSource><PMID Version="1">8528010</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Bacteriol. 2001 Jan;183(1):214-20</RefSource><PMID Version="1">11114919</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D001409" MajorTopicYN="N">Bacillus cereus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015373" MajorTopicYN="N">Bacterial Typing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002957" MajorTopicYN="N">Citrus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024881" MajorTopicYN="N">Drug Resistance, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020563" MajorTopicYN="N">Gammaproteobacteria</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC126398</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>9</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>2</Month><Day>7</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>9</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12324338</ArticleId><ArticleId IdType="pmc">PMC126398</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><noCountry><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name><name sortKey="Maccheroni, Walter" sort="Maccheroni, Walter" uniqKey="Maccheroni W" first="Walter" last="Maccheroni">Walter Maccheroni</name><name sortKey="Marcon, Joelma" sort="Marcon, Joelma" uniqKey="Marcon J" first="Joelma" last="Marcon">Joelma Marcon</name><name sortKey="Van Elsas, Jan Dirk" sort="Van Elsas, Jan Dirk" uniqKey="Van Elsas J" first="Jan Dirk" last="Van Elsas">Jan Dirk Van Elsas</name><name sortKey="Van Vuurde, Jim W L" sort="Van Vuurde, Jim W L" uniqKey="Van Vuurde J" first="Jim W L" last="Van Vuurde">Jim W L. Van Vuurde</name></noCountry><country name="Brésil"><region name="État de São Paulo"><name sortKey="Araujo, Welington L" sort="Araujo, Welington L" uniqKey="Araujo W" first="Welington L" last="Araújo">Welington L. Araújo</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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