Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus
Identifieur interne : 000992 ( PascalFrancis/Checkpoint ); précédent : 000991; suivant : 000993Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus
Auteurs : O. Avsian-Kretchmer [Israël] ; Y. Eshdat [Israël] ; Y. Gueta-Dahan [Israël] ; G. Ben-Hayyim [Israël]Source :
- Planta [ 0032-0935 ] ; 1999.
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- Pascal (Inist)
English descriptors
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Abstract
Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus</title><author><name sortKey="Avsian Kretchmer, O" sort="Avsian Kretchmer, O" uniqKey="Avsian Kretchmer O" first="O." last="Avsian-Kretchmer">O. Avsian-Kretchmer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Eshdat, Y" sort="Eshdat, Y" uniqKey="Eshdat Y" first="Y." last="Eshdat">Y. Eshdat</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Gueta Dahan, Y" sort="Gueta Dahan, Y" uniqKey="Gueta Dahan Y" first="Y." last="Gueta-Dahan">Y. Gueta-Dahan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Ben Hayyim, G" sort="Ben Hayyim, G" uniqKey="Ben Hayyim G" first="G." last="Ben-Hayyim">G. Ben-Hayyim</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">00-0005958</idno><date when="1999">1999</date><idno type="stanalyst">PASCAL 00-0005958 INIST</idno><idno type="RBID">Pascal:00-0005958</idno><idno type="wicri:Area/PascalFrancis/Corpus">000A04</idno><idno type="wicri:Area/PascalFrancis/Curation">000000</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000992</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus</title><author><name sortKey="Avsian Kretchmer, O" sort="Avsian Kretchmer, O" uniqKey="Avsian Kretchmer O" first="O." last="Avsian-Kretchmer">O. Avsian-Kretchmer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Eshdat, Y" sort="Eshdat, Y" uniqKey="Eshdat Y" first="Y." last="Eshdat">Y. Eshdat</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Gueta Dahan, Y" sort="Gueta Dahan, Y" uniqKey="Gueta Dahan Y" first="Y." last="Gueta-Dahan">Y. Gueta-Dahan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Ben Hayyim, G" sort="Ben Hayyim, G" uniqKey="Ben Hayyim G" first="G." last="Ben-Hayyim">G. Ben-Hayyim</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Planta</title><title level="j" type="abbreviated">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="1999">1999</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Planta</title><title level="j" type="abbreviated">Planta</title><idno type="ISSN">0032-0935</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic acid</term><term>Antioxidant</term><term>Citrus sinensis</term><term>Defense mechanism</term><term>Gene expression</term><term>Induction</term><term>Oxidative stress</term><term>Peroxidase</term><term>Regulation(control)</term><term>Salinity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Régulation</term><term>Stress oxydatif</term><term>Induction</term><term>Expression génique</term><term>Peroxidase</term><term>Antioxydant</term><term>Salinité</term><term>Mécanisme défense</term><term>Citrus sinensis</term><term>Abscissique acide</term><term>Phospholipid hydroperoxide glutathione peroxidase</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0032-0935</s0></fA01><fA02 i1="01"><s0>PLANAB</s0></fA02><fA03 i2="1"><s0>Planta</s0></fA03><fA05><s2>209</s2></fA05><fA06><s2>4</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus</s1></fA08><fA11 i1="01" i2="1"><s1>AVSIAN-KRETCHMER (O.)</s1></fA11><fA11 i1="02" i2="1"><s1>ESHDAT (Y.)</s1></fA11><fA11 i1="03" i2="1"><s1>GUETA-DAHAN (Y.)</s1></fA11><fA11 i1="04" i2="1"><s1>BEN-HAYYIM (G.)</s1></fA11><fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></fA14><fA20><s1>469-477</s1></fA20><fA21><s1>1999</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>916</s2><s5>354000080298530110</s5></fA43><fA44><s0>0000</s0><s1>© 2000 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1 p.1/2</s0></fA45><fA47 i1="01" i2="1"><s0>00-0005958</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Planta</s0></fA64><fA66 i1="01"><s0>DEU</s0></fA66><fC01 i1="01" l="ENG"><s0>Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.</s0></fC01><fC02 i1="01" i2="X"><s0>002A32D01B4</s0></fC02><fC02 i1="02" i2="X"><s0>002A34D01B</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Régulation</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Regulation(control)</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Regulación</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Stress oxydatif</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Oxidative stress</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Stress oxidativo</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Induction</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Induction</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Inducción</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Expression génique</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Gene expression</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Expresión genética</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Peroxidase</s0><s2>FE</s2><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Peroxidase</s0><s2>FE</s2><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Peroxidase</s0><s2>FE</s2><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Antioxydant</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Antioxidant</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Antioxidante</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Salinité</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Salinity</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Salinidad</s0><s5>07</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Mécanisme défense</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Defense mechanism</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Mecanismo defensa</s0><s5>08</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Citrus sinensis</s0><s2>NS</s2><s5>10</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Citrus sinensis</s0><s2>NS</s2><s5>10</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Citrus sinensis</s0><s2>NS</s2><s5>10</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Abscissique acide</s0><s2>NK</s2><s2>FF</s2><s5>15</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Abscisic acid</s0><s2>NK</s2><s2>FF</s2><s5>15</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Abscísico ácido</s0><s2>NK</s2><s2>FF</s2><s5>15</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Phospholipid hydroperoxide glutathione peroxidase</s0><s2>FE</s2><s4>INC</s4><s5>68</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Peroxidases</s0><s2>FE</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Peroxidases</s0><s2>FE</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Peroxidases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Oxidoreductases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Oxidoreductases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Oxidoreductases</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Enzyme</s0></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Enzyme</s0></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Enzima</s0></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Rutaceae</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Rutaceae</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Rutaceae</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Dicotyledones</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Dicotyledones</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Dicotyledones</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Angiospermae</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Angiospermae</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Angiospermae</s0><s2>NS</s2></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Spermatophyta</s0><s2>NS</s2></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Spermatophyta</s0><s2>NS</s2></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Spermatophyta</s0><s2>NS</s2></fC07><fC07 i1="08" i2="X" l="FRE"><s0>Agrume</s0><s5>40</s5></fC07><fC07 i1="08" i2="X" l="ENG"><s0>Citrus fruit</s0><s5>40</s5></fC07><fC07 i1="08" i2="X" l="SPA"><s0>Agrios</s0><s5>40</s5></fC07><fN21><s1>003</s1></fN21></pA></standard></inist><affiliations><list><country><li>Israël</li></country></list><tree><country name="Israël"><noRegion><name sortKey="Avsian Kretchmer, O" sort="Avsian Kretchmer, O" uniqKey="Avsian Kretchmer O" first="O." last="Avsian-Kretchmer">O. Avsian-Kretchmer</name></noRegion><name sortKey="Ben Hayyim, G" sort="Ben Hayyim, G" uniqKey="Ben Hayyim G" first="G." last="Ben-Hayyim">G. Ben-Hayyim</name><name sortKey="Eshdat, Y" sort="Eshdat, Y" uniqKey="Eshdat Y" first="Y." last="Eshdat">Y. Eshdat</name><name sortKey="Gueta Dahan, Y" sort="Gueta Dahan, Y" uniqKey="Gueta Dahan Y" first="Y." last="Gueta-Dahan">Y. Gueta-Dahan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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