OrangerV1, PascalFrancis, Corpus, bibRecord, 000A04

Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus

Identifieur interne : 000A04 ( PascalFrancis/Corpus ); précédent : 000A03; suivant : 000A05

Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus

Auteurs : O. Avsian-Kretchmer ; Y. Eshdat ; Y. Gueta-Dahan ; G. Ben-Hayyim

Source :

Planta [ 0032-0935 ] ; 1999.

RBID : Pascal:00-0005958

Descripteurs français

Pascal (Inist)
- Régulation, Stress oxydatif, Induction, Expression génique, Peroxidase, Antioxydant, Salinité, Mécanisme défense, Citrus sinensis, Abscissique acide, Phospholipid hydroperoxide glutathione peroxidase.

English descriptors

KwdEn :
- Abscisic acid, Antioxidant, Citrus sinensis, Defense mechanism, Gene expression, Induction, Oxidative stress, Peroxidase, Regulation(control), Salinity.

Abstract

Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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Format Inist (serveur)

NO :	PASCAL 00-0005958 INIST
ET :	Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus
AU :	AVSIAN-KRETCHMER (O.); ESHDAT (Y.); GUETA-DAHAN (Y.); BEN-HAYYIM (G.)
AF :	Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6/Bet Dagan, 50250/Israël (1 aut., 2 aut., 3 aut., 4 aut.)
DT :	Publication en série; Niveau analytique
SO :	Planta; ISSN 0032-0935; Coden PLANAB; Allemagne; Da. 1999; Vol. 209; No. 4; Pp. 469-477; Bibl. 1 p.1/2
LA :	Anglais
EA :	Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.
CC :	002A32D01B4; 002A34D01B
FD :	Régulation; Stress oxydatif; Induction; Expression génique; Peroxidase; Antioxydant; Salinité; Mécanisme défense; Citrus sinensis; Abscissique acide; Phospholipid hydroperoxide glutathione peroxidase
FG :	Peroxidases; Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume
ED :	Regulation(control); Oxidative stress; Induction; Gene expression; Peroxidase; Antioxidant; Salinity; Defense mechanism; Citrus sinensis; Abscisic acid
EG :	Peroxidases; Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit
SD :	Regulación; Stress oxidativo; Inducción; Expresión genética; Peroxidase; Antioxidante; Salinidad; Mecanismo defensa; Citrus sinensis; Abscísico ácido
LO :	INIST-916.354000080298530110
ID :	00-0005958

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Pascal:00-0005958

Le document en format XML

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<front><div type="abstract" xml:lang="en">Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.</div>
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<fC01 i1="01" l="ENG"><s0>Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.</s0>
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<fC03 i1="02" i2="X" l="FRE"><s0>Stress oxydatif</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Oxidative stress</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Stress oxidativo</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Induction</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Induction</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Inducción</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Expression génique</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Gene expression</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Expresión genética</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Peroxidase</s0>
<s2>FE</s2>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Peroxidase</s0>
<s2>FE</s2>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Peroxidase</s0>
<s2>FE</s2>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Antioxydant</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Antioxidant</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Antioxidante</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Salinité</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Salinity</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Salinidad</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Mécanisme défense</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Defense mechanism</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Mecanismo defensa</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Abscissique acide</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Abscisic acid</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Abscísico ácido</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Phospholipid hydroperoxide glutathione peroxidase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>68</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Peroxidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Peroxidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Peroxidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Enzyme</s0>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Enzyme</s0>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Enzima</s0>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Agrume</s0>
<s5>40</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>40</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Agrios</s0>
<s5>40</s5>
</fC07>
<fN21><s1>003</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 00-0005958 INIST</NO>
<ET>Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus</ET>
<AU>AVSIAN-KRETCHMER (O.); ESHDAT (Y.); GUETA-DAHAN (Y.); BEN-HAYYIM (G.)</AU>
<AF>Department of Fruit-Tree Breeding and Molecular Genetics. Agricultural Research Organization, The Volcani Center. P.O. Box 6/Bet Dagan, 50250/Israël (1 aut., 2 aut., 3 aut., 4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Planta; ISSN 0032-0935; Coden PLANAB; Allemagne; Da. 1999; Vol. 209; No. 4; Pp. 469-477; Bibl. 1 p.1/2</SO>
<LA>Anglais</LA>
<EA>Recent findings in our laboratory showed that in citrus cells, salt treatment induced the accumulation of mRNA and a protein corresponding to phospholipid hydroperoxide glutathione peroxidase (PHGPX), an enzyme active in the cellular antioxidant system. The protein and its encoding gene, csa, were isolated and characterized, and the expected enzymatic activity was demonstrated (G. Ben-Hayyim et al., 1993, Plant Sci. 88: 129-140; D. Holland et al., 1993, Plant Mol. Biol. 21: 923-927; D. Holland et al., 1994, FEBS Lett. 337: 52-55; T. Beeor-Tzahar et al., 1995, FEBS Lett. 366: 151-155). In an attempt to find out how salt induces the expression of an antioxidant enzyme, the regulation of PHGPX in citrus cells was studied at both the mRNA transcript and the protein levels. A high and transient response at the csa mRNA level was observed after 4-7 h of exposing salt-sensitive cells to NaCI, or abscisic acid, whereas no response could be detected in the salt-tolerant cells under the same conditions, tert-Butylhydroperoxide, a substrate of PHGPX, induced csa mRNA transcripts after only 2 h, and abolished the differential response between salt-sensitive and salt-tolerant cells. On the basis of these results and those obtained under heat and cold stresses, it is suggested that csa is directly induced by the substrate of its encoded enzyme PHGPX, and that salt induction occurs mainly via the production of reactive oxygen species and hydroperoxides.</EA>
<CC>002A32D01B4; 002A34D01B</CC>
<FD>Régulation; Stress oxydatif; Induction; Expression génique; Peroxidase; Antioxydant; Salinité; Mécanisme défense; Citrus sinensis; Abscissique acide; Phospholipid hydroperoxide glutathione peroxidase</FD>
<FG>Peroxidases; Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume</FG>
<ED>Regulation(control); Oxidative stress; Induction; Gene expression; Peroxidase; Antioxidant; Salinity; Defense mechanism; Citrus sinensis; Abscisic acid</ED>
<EG>Peroxidases; Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit</EG>
<SD>Regulación; Stress oxidativo; Inducción; Expresión genética; Peroxidase; Antioxidante; Salinidad; Mecanismo defensa; Citrus sinensis; Abscísico ácido</SD>
<LO>INIST-916.354000080298530110</LO>
<ID>00-0005958</ID>
</server>
</inist>
</record>

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Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus

Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in citrus

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