Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
Identifieur interne : 001732 ( Ncbi/Merge ); précédent : 001731; suivant : 001733Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
Auteurs : Tapati Bhanja Dey [Inde] ; Rintu Banerjee [Inde]Source :
- Brazilian Journal of Microbiology [ 1517-8382 ] ; 2014.
English descriptors
- KwdEn :
- Aspergillus (enzymology), Aspergillus (growth & development), Beverages, Culture Media (chemistry), Food Handling (methods), Free Radical Scavengers (analysis), Phenols (analysis), Polygalacturonase (isolation & purification), Polygalacturonase (metabolism), Temperature, Time Factors, alpha-Amylases (isolation & purification), alpha-Amylases (metabolism).
- MESH :
- chemical , analysis : Free Radical Scavengers, Phenols.
- chemical , chemistry : Culture Media.
- enzymology : Aspergillus.
- growth & development : Aspergillus.
- chemical , isolation & purification : Polygalacturonase, alpha-Amylases.
- chemical , metabolism : Polygalacturonase, alpha-Amylases.
- methods : Food Handling.
- Beverages, Temperature, Time Factors.
Abstract
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by
Url:
PubMed: 24948919
PubMed Central: 4059332
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000E66
- to stream Pmc, to step Curation: 000E65
- to stream Pmc, to step Checkpoint: 000899
- to stream PubMed, to step Corpus: 000338
- to stream PubMed, to step Curation: 000338
- to stream PubMed, to step Checkpoint: 000338
Links to Exploration step
PMC:4059332Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification</title>
<author><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff02"><institution content-type="orgdiv4">Lignocellulose Biotechnology Laboratory</institution>
<institution content-type="orgdiv5">Department of Microbiology</institution>
<institution content-type="orgdiv6">University of Delhi South Campus</institution>
<addr-line><named-content content-type="city">Benito Juarez Road</named-content>
<named-content content-type="state">New Delhi</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24948919</idno>
<idno type="pmc">4059332</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059332</idno>
<idno type="RBID">PMC:4059332</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000E66</idno>
<idno type="wicri:Area/Pmc/Curation">000E65</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000899</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">000338</idno>
<idno type="wicri:Area/PubMed/Curation">000338</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000338</idno>
<idno type="wicri:Area/Ncbi/Merge">001732</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification</title>
<author><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff02"><institution content-type="orgdiv4">Lignocellulose Biotechnology Laboratory</institution>
<institution content-type="orgdiv5">Department of Microbiology</institution>
<institution content-type="orgdiv6">University of Delhi South Campus</institution>
<addr-line><named-content content-type="city">Benito Juarez Road</named-content>
<named-content content-type="state">New Delhi</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Brazilian Journal of Microbiology</title>
<idno type="ISSN">1517-8382</idno>
<idno type="eISSN">1678-4405</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aspergillus (enzymology)</term>
<term>Aspergillus (growth & development)</term>
<term>Beverages</term>
<term>Culture Media (chemistry)</term>
<term>Food Handling (methods)</term>
<term>Free Radical Scavengers (analysis)</term>
<term>Phenols (analysis)</term>
<term>Polygalacturonase (isolation & purification)</term>
<term>Polygalacturonase (metabolism)</term>
<term>Temperature</term>
<term>Time Factors</term>
<term>alpha-Amylases (isolation & purification)</term>
<term>alpha-Amylases (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Free Radical Scavengers</term>
<term>Phenols</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Polygalacturonase</term>
<term>alpha-Amylases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Polygalacturonase</term>
<term>alpha-Amylases</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Food Handling</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Beverages</term>
<term>Temperature</term>
<term>Time Factors</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by <italic>Aspergillus awamori</italic>
Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (<italic>Citrus sinensis</italic>
var mosambi) peels as solid substrate whereas, α-amylase was produced from <italic>A. oryzae</italic>
(IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum clarity (%T<sub>660nm</sub>
= 97.0%) of juice was attained after 2 h of incubation at 50 °C in presence of 10 mM CaCl<sub>2</sub>
. Total phenolic content of juice was reduced by 19.8% after clarification, yet with slightly higher %DPPH radical scavenging property.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aikat, K" uniqKey="Aikat K">K Aikat</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aikat, K" uniqKey="Aikat K">K Aikat</name>
</author>
<author><name sortKey="Maiti, Tk" uniqKey="Maiti T">TK Maiti</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alvarez, S" uniqKey="Alvarez S">S Alvarez</name>
</author>
<author><name sortKey="Alvarez, R" uniqKey="Alvarez R">R Alvarez</name>
</author>
<author><name sortKey="Riera, Fa" uniqKey="Riera F">FA Riera</name>
</author>
<author><name sortKey="Coca, J" uniqKey="Coca J">J Coca</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benitez, Ei" uniqKey="Benitez E">EI Benitez</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bhanja, T" uniqKey="Bhanja T">T Bhanja</name>
</author>
<author><name sortKey="Rout, S" uniqKey="Rout S">S Rout</name>
</author>
<author><name sortKey="Banerjee, R" uniqKey="Banerjee R">R Banerjee</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bhanja, T" uniqKey="Bhanja T">T Bhanja</name>
</author>
<author><name sortKey="Rout, S" uniqKey="Rout S">S Rout</name>
</author>
<author><name sortKey="Banerjee, R" uniqKey="Banerjee R">R Banerjee</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Borneman, Z" uniqKey="Borneman Z">Z Borneman</name>
</author>
<author><name sortKey="Gokmen, V" uniqKey="Gokmen V">V Gokmen</name>
</author>
<author><name sortKey="Nijhuis, Hh" uniqKey="Nijhuis H">HH Nijhuis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brand Williams, W" uniqKey="Brand Williams W">W Brand-Williams</name>
</author>
<author><name sortKey="Cuvelier, Me" uniqKey="Cuvelier M">ME Cuvelier</name>
</author>
<author><name sortKey="Berset, C" uniqKey="Berset C">C Berset</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Busto, Md" uniqKey="Busto M">MD Busto</name>
</author>
<author><name sortKey="Garcia Tramontin, Ke" uniqKey="Garcia Tramontin K">KE Garcia-Tramontin</name>
</author>
<author><name sortKey="Ortega, N" uniqKey="Ortega N">N Ortega</name>
</author>
<author><name sortKey="Perez Mateos, M" uniqKey="Perez Mateos M">M Perez-Mateos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carrin, Me" uniqKey="Carrin M">ME Carrin</name>
</author>
<author><name sortKey="Ceci, Ln" uniqKey="Ceci L">LN Ceci</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ceci, Ln" uniqKey="Ceci L">LN Ceci</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ceci, Ln" uniqKey="Ceci L">LN Ceci</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dey, Tb" uniqKey="Dey T">TB Dey</name>
</author>
<author><name sortKey="Banerjee, R" uniqKey="Banerjee R">R Banerjee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Diano, N" uniqKey="Diano N">N Diano</name>
</author>
<author><name sortKey="Grimaldi, T" uniqKey="Grimaldi T">T Grimaldi</name>
</author>
<author><name sortKey="Bianco, M" uniqKey="Bianco M">M Bianco</name>
</author>
<author><name sortKey="Rossi, S" uniqKey="Rossi S">S Rossi</name>
</author>
<author><name sortKey="Gabrovska, K" uniqKey="Gabrovska K">K Gabrovska</name>
</author>
<author><name sortKey="Yordanova, G" uniqKey="Yordanova G">G Yordanova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Emmons, Cl" uniqKey="Emmons C">CL Emmons</name>
</author>
<author><name sortKey="Peterson, Dm" uniqKey="Peterson D">DM Peterson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Floribeth, V" uniqKey="Floribeth V">V Floribeth</name>
</author>
<author><name sortKey="Celsa, L" uniqKey="Celsa L">L Celsa</name>
</author>
<author><name sortKey="Cooke, Rd" uniqKey="Cooke R">RD Cooke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grampp, E" uniqKey="Grampp E">E Grampp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gutcho, Sj" uniqKey="Gutcho S">SJ Gutcho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hutchings, Jb" uniqKey="Hutchings J">JB Hutchings</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ishii, S" uniqKey="Ishii S">S Ishii</name>
</author>
<author><name sortKey="Yokotsuka, T" uniqKey="Yokotsuka T">T Yokotsuka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kahle, K" uniqKey="Kahle K">K Kahle</name>
</author>
<author><name sortKey="Kraus, M" uniqKey="Kraus M">M Kraus</name>
</author>
<author><name sortKey="Richling, E" uniqKey="Richling E">E Richling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kashyap, Dr" uniqKey="Kashyap D">DR Kashyap</name>
</author>
<author><name sortKey="Vohra, Pk" uniqKey="Vohra P">PK Vohra</name>
</author>
<author><name sortKey="Chopra, S" uniqKey="Chopra S">S Chopra</name>
</author>
<author><name sortKey="Tewari, R" uniqKey="Tewari R">R Tewari</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumar, Cgp" uniqKey="Kumar C">CGP Kumar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miller, Gl" uniqKey="Miller G">GL Miller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murthy, Ps" uniqKey="Murthy P">PS Murthy</name>
</author>
<author><name sortKey="Naidu, Mm" uniqKey="Naidu M">MM Naidu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nakkeeran, E" uniqKey="Nakkeeran E">E Nakkeeran</name>
</author>
<author><name sortKey="Kumar, Su" uniqKey="Kumar S">SU Kumar</name>
</author>
<author><name sortKey="Subramanian, R" uniqKey="Subramanian R">R Subramanian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oszmianski, J" uniqKey="Oszmianski J">J Oszmianski</name>
</author>
<author><name sortKey="Wojdylo, A" uniqKey="Wojdylo A">A Wojdylo</name>
</author>
<author><name sortKey="Kolniak, J" uniqKey="Kolniak J">J Kolniak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pradhan, Bk" uniqKey="Pradhan B">BK Pradhan</name>
</author>
<author><name sortKey="Sandle, Nk" uniqKey="Sandle N">NK Sandle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sahnoun, M" uniqKey="Sahnoun M">M Sahnoun</name>
</author>
<author><name sortKey="Bejar, S" uniqKey="Bejar S">S Bejar</name>
</author>
<author><name sortKey="Sayari, A" uniqKey="Sayari A">A Sayari</name>
</author>
<author><name sortKey="Triki, Ma" uniqKey="Triki M">MA Triki</name>
</author>
<author><name sortKey="Kriaa, M" uniqKey="Kriaa M">M Kriaa</name>
</author>
<author><name sortKey="Kammoun, R" uniqKey="Kammoun R">R Kammoun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Saxena, S" uniqKey="Saxena S">S Saxena</name>
</author>
<author><name sortKey="Shukla, S" uniqKey="Shukla S">S Shukla</name>
</author>
<author><name sortKey="Thakur, A" uniqKey="Thakur A">A Thakur</name>
</author>
<author><name sortKey="Gupta, R" uniqKey="Gupta R">R Gupta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Singh, S" uniqKey="Singh S">S Singh</name>
</author>
<author><name sortKey="Gupta, R" uniqKey="Gupta R">R Gupta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Szajer, I" uniqKey="Szajer I">I Szajer</name>
</author>
<author><name sortKey="Szajer, C" uniqKey="Szajer C">C Szajer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Urlaub, R" uniqKey="Urlaub R">R Urlaub</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vaillant, F" uniqKey="Vaillant F">F Vaillant</name>
</author>
<author><name sortKey="Millan, A" uniqKey="Millan A">A Millan</name>
</author>
<author><name sortKey="Dornier, M" uniqKey="Dornier M">M Dornier</name>
</author>
<author><name sortKey="Decloux, M" uniqKey="Decloux M">M Decloux</name>
</author>
<author><name sortKey="Reynes, M" uniqKey="Reynes M">M Reynes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Youn, Ks" uniqKey="Youn K">KS Youn</name>
</author>
<author><name sortKey="Hong, Jh" uniqKey="Hong J">JH Hong</name>
</author>
<author><name sortKey="Bae, Dh" uniqKey="Bae D">DH Bae</name>
</author>
<author><name sortKey="Kim, Sj" uniqKey="Kim S">SJ Kim</name>
</author>
<author><name sortKey="Kim, Sd" uniqKey="Kim S">SD Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yuan, P" uniqKey="Yuan P">P Yuan</name>
</author>
<author><name sortKey="Meng, K" uniqKey="Meng K">K Meng</name>
</author>
<author><name sortKey="Huang, H" uniqKey="Huang H">H Huang</name>
</author>
<author><name sortKey="Shi, P" uniqKey="Shi P">P Shi</name>
</author>
<author><name sortKey="Luo, H" uniqKey="Luo H">H Luo</name>
</author>
<author><name sortKey="Yang, P" uniqKey="Yang P">P Yang</name>
</author>
<author><name sortKey="Yao, B" uniqKey="Yao B">B Yao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yusof, S" uniqKey="Yusof S">S Yusof</name>
</author>
<author><name sortKey="Nurzarina, I" uniqKey="Nurzarina I">I Nurzarina</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<double pmid="24948919"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification</title>
<author><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff02"><institution content-type="orgdiv4">Lignocellulose Biotechnology Laboratory</institution>
<institution content-type="orgdiv5">Department of Microbiology</institution>
<institution content-type="orgdiv6">University of Delhi South Campus</institution>
<addr-line><named-content content-type="city">Benito Juarez Road</named-content>
<named-content content-type="state">New Delhi</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24948919</idno>
<idno type="pmc">4059332</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059332</idno>
<idno type="RBID">PMC:4059332</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000E66</idno>
<idno type="wicri:Area/Pmc/Curation">000E65</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000899</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification</title>
<author><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff02"><institution content-type="orgdiv4">Lignocellulose Biotechnology Laboratory</institution>
<institution content-type="orgdiv5">Department of Microbiology</institution>
<institution content-type="orgdiv6">University of Delhi South Campus</institution>
<addr-line><named-content content-type="city">Benito Juarez Road</named-content>
<named-content content-type="state">New Delhi</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
<affiliation wicri:level="1"><nlm:aff id="aff01"><institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Brazilian Journal of Microbiology</title>
<idno type="ISSN">1517-8382</idno>
<idno type="eISSN">1678-4405</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by <italic>Aspergillus awamori</italic>
Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (<italic>Citrus sinensis</italic>
var mosambi) peels as solid substrate whereas, α-amylase was produced from <italic>A. oryzae</italic>
(IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum clarity (%T<sub>660nm</sub>
= 97.0%) of juice was attained after 2 h of incubation at 50 °C in presence of 10 mM CaCl<sub>2</sub>
. Total phenolic content of juice was reduced by 19.8% after clarification, yet with slightly higher %DPPH radical scavenging property.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aikat, K" uniqKey="Aikat K">K Aikat</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aikat, K" uniqKey="Aikat K">K Aikat</name>
</author>
<author><name sortKey="Maiti, Tk" uniqKey="Maiti T">TK Maiti</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alvarez, S" uniqKey="Alvarez S">S Alvarez</name>
</author>
<author><name sortKey="Alvarez, R" uniqKey="Alvarez R">R Alvarez</name>
</author>
<author><name sortKey="Riera, Fa" uniqKey="Riera F">FA Riera</name>
</author>
<author><name sortKey="Coca, J" uniqKey="Coca J">J Coca</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benitez, Ei" uniqKey="Benitez E">EI Benitez</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bhanja, T" uniqKey="Bhanja T">T Bhanja</name>
</author>
<author><name sortKey="Rout, S" uniqKey="Rout S">S Rout</name>
</author>
<author><name sortKey="Banerjee, R" uniqKey="Banerjee R">R Banerjee</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bhanja, T" uniqKey="Bhanja T">T Bhanja</name>
</author>
<author><name sortKey="Rout, S" uniqKey="Rout S">S Rout</name>
</author>
<author><name sortKey="Banerjee, R" uniqKey="Banerjee R">R Banerjee</name>
</author>
<author><name sortKey="Bhattacharyya, Bc" uniqKey="Bhattacharyya B">BC Bhattacharyya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Borneman, Z" uniqKey="Borneman Z">Z Borneman</name>
</author>
<author><name sortKey="Gokmen, V" uniqKey="Gokmen V">V Gokmen</name>
</author>
<author><name sortKey="Nijhuis, Hh" uniqKey="Nijhuis H">HH Nijhuis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brand Williams, W" uniqKey="Brand Williams W">W Brand-Williams</name>
</author>
<author><name sortKey="Cuvelier, Me" uniqKey="Cuvelier M">ME Cuvelier</name>
</author>
<author><name sortKey="Berset, C" uniqKey="Berset C">C Berset</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Busto, Md" uniqKey="Busto M">MD Busto</name>
</author>
<author><name sortKey="Garcia Tramontin, Ke" uniqKey="Garcia Tramontin K">KE Garcia-Tramontin</name>
</author>
<author><name sortKey="Ortega, N" uniqKey="Ortega N">N Ortega</name>
</author>
<author><name sortKey="Perez Mateos, M" uniqKey="Perez Mateos M">M Perez-Mateos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carrin, Me" uniqKey="Carrin M">ME Carrin</name>
</author>
<author><name sortKey="Ceci, Ln" uniqKey="Ceci L">LN Ceci</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ceci, Ln" uniqKey="Ceci L">LN Ceci</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ceci, Ln" uniqKey="Ceci L">LN Ceci</name>
</author>
<author><name sortKey="Lozano, Je" uniqKey="Lozano J">JE Lozano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dey, Tb" uniqKey="Dey T">TB Dey</name>
</author>
<author><name sortKey="Banerjee, R" uniqKey="Banerjee R">R Banerjee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Diano, N" uniqKey="Diano N">N Diano</name>
</author>
<author><name sortKey="Grimaldi, T" uniqKey="Grimaldi T">T Grimaldi</name>
</author>
<author><name sortKey="Bianco, M" uniqKey="Bianco M">M Bianco</name>
</author>
<author><name sortKey="Rossi, S" uniqKey="Rossi S">S Rossi</name>
</author>
<author><name sortKey="Gabrovska, K" uniqKey="Gabrovska K">K Gabrovska</name>
</author>
<author><name sortKey="Yordanova, G" uniqKey="Yordanova G">G Yordanova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Emmons, Cl" uniqKey="Emmons C">CL Emmons</name>
</author>
<author><name sortKey="Peterson, Dm" uniqKey="Peterson D">DM Peterson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Floribeth, V" uniqKey="Floribeth V">V Floribeth</name>
</author>
<author><name sortKey="Celsa, L" uniqKey="Celsa L">L Celsa</name>
</author>
<author><name sortKey="Cooke, Rd" uniqKey="Cooke R">RD Cooke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grampp, E" uniqKey="Grampp E">E Grampp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gutcho, Sj" uniqKey="Gutcho S">SJ Gutcho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hutchings, Jb" uniqKey="Hutchings J">JB Hutchings</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ishii, S" uniqKey="Ishii S">S Ishii</name>
</author>
<author><name sortKey="Yokotsuka, T" uniqKey="Yokotsuka T">T Yokotsuka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kahle, K" uniqKey="Kahle K">K Kahle</name>
</author>
<author><name sortKey="Kraus, M" uniqKey="Kraus M">M Kraus</name>
</author>
<author><name sortKey="Richling, E" uniqKey="Richling E">E Richling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kashyap, Dr" uniqKey="Kashyap D">DR Kashyap</name>
</author>
<author><name sortKey="Vohra, Pk" uniqKey="Vohra P">PK Vohra</name>
</author>
<author><name sortKey="Chopra, S" uniqKey="Chopra S">S Chopra</name>
</author>
<author><name sortKey="Tewari, R" uniqKey="Tewari R">R Tewari</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumar, Cgp" uniqKey="Kumar C">CGP Kumar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miller, Gl" uniqKey="Miller G">GL Miller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murthy, Ps" uniqKey="Murthy P">PS Murthy</name>
</author>
<author><name sortKey="Naidu, Mm" uniqKey="Naidu M">MM Naidu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nakkeeran, E" uniqKey="Nakkeeran E">E Nakkeeran</name>
</author>
<author><name sortKey="Kumar, Su" uniqKey="Kumar S">SU Kumar</name>
</author>
<author><name sortKey="Subramanian, R" uniqKey="Subramanian R">R Subramanian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oszmianski, J" uniqKey="Oszmianski J">J Oszmianski</name>
</author>
<author><name sortKey="Wojdylo, A" uniqKey="Wojdylo A">A Wojdylo</name>
</author>
<author><name sortKey="Kolniak, J" uniqKey="Kolniak J">J Kolniak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pradhan, Bk" uniqKey="Pradhan B">BK Pradhan</name>
</author>
<author><name sortKey="Sandle, Nk" uniqKey="Sandle N">NK Sandle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sahnoun, M" uniqKey="Sahnoun M">M Sahnoun</name>
</author>
<author><name sortKey="Bejar, S" uniqKey="Bejar S">S Bejar</name>
</author>
<author><name sortKey="Sayari, A" uniqKey="Sayari A">A Sayari</name>
</author>
<author><name sortKey="Triki, Ma" uniqKey="Triki M">MA Triki</name>
</author>
<author><name sortKey="Kriaa, M" uniqKey="Kriaa M">M Kriaa</name>
</author>
<author><name sortKey="Kammoun, R" uniqKey="Kammoun R">R Kammoun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Saxena, S" uniqKey="Saxena S">S Saxena</name>
</author>
<author><name sortKey="Shukla, S" uniqKey="Shukla S">S Shukla</name>
</author>
<author><name sortKey="Thakur, A" uniqKey="Thakur A">A Thakur</name>
</author>
<author><name sortKey="Gupta, R" uniqKey="Gupta R">R Gupta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Singh, S" uniqKey="Singh S">S Singh</name>
</author>
<author><name sortKey="Gupta, R" uniqKey="Gupta R">R Gupta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Szajer, I" uniqKey="Szajer I">I Szajer</name>
</author>
<author><name sortKey="Szajer, C" uniqKey="Szajer C">C Szajer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Urlaub, R" uniqKey="Urlaub R">R Urlaub</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vaillant, F" uniqKey="Vaillant F">F Vaillant</name>
</author>
<author><name sortKey="Millan, A" uniqKey="Millan A">A Millan</name>
</author>
<author><name sortKey="Dornier, M" uniqKey="Dornier M">M Dornier</name>
</author>
<author><name sortKey="Decloux, M" uniqKey="Decloux M">M Decloux</name>
</author>
<author><name sortKey="Reynes, M" uniqKey="Reynes M">M Reynes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Youn, Ks" uniqKey="Youn K">KS Youn</name>
</author>
<author><name sortKey="Hong, Jh" uniqKey="Hong J">JH Hong</name>
</author>
<author><name sortKey="Bae, Dh" uniqKey="Bae D">DH Bae</name>
</author>
<author><name sortKey="Kim, Sj" uniqKey="Kim S">SJ Kim</name>
</author>
<author><name sortKey="Kim, Sd" uniqKey="Kim S">SD Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yuan, P" uniqKey="Yuan P">P Yuan</name>
</author>
<author><name sortKey="Meng, K" uniqKey="Meng K">K Meng</name>
</author>
<author><name sortKey="Huang, H" uniqKey="Huang H">H Huang</name>
</author>
<author><name sortKey="Shi, P" uniqKey="Shi P">P Shi</name>
</author>
<author><name sortKey="Luo, H" uniqKey="Luo H">H Luo</name>
</author>
<author><name sortKey="Yang, P" uniqKey="Yang P">P Yang</name>
</author>
<author><name sortKey="Yao, B" uniqKey="Yao B">B Yao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yusof, S" uniqKey="Yusof S">S Yusof</name>
</author>
<author><name sortKey="Nurzarina, I" uniqKey="Nurzarina I">I Nurzarina</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</pmc>
<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification.</title>
<author><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
<affiliation><nlm:affiliation>Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India ; Lignocellulose Biotechnology Laboratory Department of Microbiology University of Delhi South Campus Benito Juarez RoadNew Delhi India.</nlm:affiliation>
<wicri:noCountry code="no comma">Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India ; Lignocellulose Biotechnology Laboratory Department of Microbiology University of Delhi South Campus Benito Juarez RoadNew Delhi India.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
<affiliation><nlm:affiliation>Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India.</nlm:affiliation>
<wicri:noCountry code="no comma">Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India.</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:24948919</idno>
<idno type="pmid">24948919</idno>
<idno type="wicri:Area/PubMed/Corpus">000338</idno>
<idno type="wicri:Area/PubMed/Curation">000338</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000338</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification.</title>
<author><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
<affiliation><nlm:affiliation>Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India ; Lignocellulose Biotechnology Laboratory Department of Microbiology University of Delhi South Campus Benito Juarez RoadNew Delhi India.</nlm:affiliation>
<wicri:noCountry code="no comma">Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India ; Lignocellulose Biotechnology Laboratory Department of Microbiology University of Delhi South Campus Benito Juarez RoadNew Delhi India.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
<affiliation><nlm:affiliation>Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India.</nlm:affiliation>
<wicri:noCountry code="no comma">Microbial Biotechnology and Downstream Processing Lab Agricultural and Food Engineering Department Indian Institute of Technology Kharagpur India.</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]</title>
<idno type="eISSN">1678-4405</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aspergillus (enzymology)</term>
<term>Aspergillus (growth & development)</term>
<term>Beverages</term>
<term>Culture Media (chemistry)</term>
<term>Food Handling (methods)</term>
<term>Free Radical Scavengers (analysis)</term>
<term>Phenols (analysis)</term>
<term>Polygalacturonase (isolation & purification)</term>
<term>Polygalacturonase (metabolism)</term>
<term>Temperature</term>
<term>Time Factors</term>
<term>alpha-Amylases (isolation & purification)</term>
<term>alpha-Amylases (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Free Radical Scavengers</term>
<term>Phenols</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Polygalacturonase</term>
<term>alpha-Amylases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Polygalacturonase</term>
<term>alpha-Amylases</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Food Handling</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Beverages</term>
<term>Temperature</term>
<term>Time Factors</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum clarity (%T(660 nm) = 97.0%) of juice was attained after 2 h of incubation at 50 °C in presence of 10 mM CaCl2. Total phenolic content of juice was reduced by 19.8% after clarification, yet with slightly higher %DPPH radical scavenging property.</div>
</front>
</TEI>
</pubmed>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001732 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001732 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:4059332 |texte= Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:24948919" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
![]() | This area was generated with Dilib version V0.6.25. | ![]() |