Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
Identifieur interne : 000899 ( Pmc/Checkpoint ); précédent : 000898; suivant : 000900Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
Auteurs : Tapati Bhanja Dey [Inde] ; Rintu Banerjee [Inde]Source :
- Brazilian Journal of Microbiology [ 1517-8382 ] ; 2014.
Abstract
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by
Url:
PubMed: 24948919
PubMed Central: 4059332
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<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
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<affiliation wicri:level="1"><nlm:aff id="aff02"><institution content-type="orgdiv4">Lignocellulose Biotechnology Laboratory</institution>
<institution content-type="orgdiv5">Department of Microbiology</institution>
<institution content-type="orgdiv6">University of Delhi South Campus</institution>
<addr-line><named-content content-type="city">Benito Juarez Road</named-content>
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<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification</title>
<author><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
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<affiliation wicri:level="1"><nlm:aff id="aff02"><institution content-type="orgdiv4">Lignocellulose Biotechnology Laboratory</institution>
<institution content-type="orgdiv5">Department of Microbiology</institution>
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<author><name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
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<series><title level="j">Brazilian Journal of Microbiology</title>
<idno type="ISSN">1517-8382</idno>
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<front><div type="abstract" xml:lang="en"><p>Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by <italic>Aspergillus awamori</italic>
Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (<italic>Citrus sinensis</italic>
var mosambi) peels as solid substrate whereas, α-amylase was produced from <italic>A. oryzae</italic>
(IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum clarity (%T<sub>660nm</sub>
= 97.0%) of juice was attained after 2 h of incubation at 50 °C in presence of 10 mM CaCl<sub>2</sub>
. Total phenolic content of juice was reduced by 19.8% after clarification, yet with slightly higher %DPPH radical scavenging property.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Braz J Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Braz. J. Microbiol</journal-id>
<journal-title-group><journal-title>Brazilian Journal of Microbiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">1517-8382</issn>
<issn pub-type="epub">1678-4405</issn>
<publisher><publisher-name>Sociedade Brasileira de Microbiologia</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24948919</article-id>
<article-id pub-id-type="pmc">4059332</article-id>
<article-id pub-id-type="publisher-id">bjm-45-97</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Dey</surname>
<given-names>Tapati Bhanja</given-names>
</name>
<xref ref-type="aff" rid="aff01">1</xref>
<xref ref-type="aff" rid="aff02">2</xref>
<xref ref-type="corresp" rid="c1-bjm-45-97"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Banerjee</surname>
<given-names>Rintu</given-names>
</name>
<xref ref-type="aff" rid="aff01">1</xref>
</contrib>
</contrib-group>
<aff id="aff01"><label>1</label>
<institution content-type="orgdiv1">Microbial Biotechnology and Downstream Processing Lab</institution>
<institution content-type="orgdiv2">Agricultural and Food Engineering Department</institution>
<institution content-type="orgdiv3">Indian Institute of Technology</institution>
<addr-line><named-content content-type="city">Kharagpur</named-content>
</addr-line>
<country>India</country>
</aff>
<aff id="aff02"><label>2</label>
<institution content-type="orgdiv4">Lignocellulose Biotechnology Laboratory</institution>
<institution content-type="orgdiv5">Department of Microbiology</institution>
<institution content-type="orgdiv6">University of Delhi South Campus</institution>
<addr-line><named-content content-type="city">Benito Juarez Road</named-content>
<named-content content-type="state">New Delhi</named-content>
</addr-line>
<country>India</country>
</aff>
<author-notes><corresp id="c1-bjm-45-97">Send correspondence to T.B. Dey. Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, 110021 New Delhi, India. E-mail: <email>tapati_bhanja@yahoo.co.in</email>
, <email>tapati.bhanja@gmail.com</email>
.</corresp>
</author-notes>
<pub-date pub-type="collection"><year>2014</year>
</pub-date>
<pub-date pub-type="epub"><day>19</day>
<month>5</month>
<year>2014</year>
</pub-date>
<volume>45</volume>
<issue>1</issue>
<fpage>97</fpage>
<lpage>104</lpage>
<history><date date-type="received"><day>12</day>
<month>10</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>04</day>
<month>4</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014, Sociedade Brasileira de Microbiologia</copyright-statement>
<copyright-year>2014</copyright-year>
<license license-type="open-access"><license-p>All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.</license-p>
</license>
</permissions>
<abstract><p>Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by <italic>Aspergillus awamori</italic>
Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (<italic>Citrus sinensis</italic>
var mosambi) peels as solid substrate whereas, α-amylase was produced from <italic>A. oryzae</italic>
(IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum clarity (%T<sub>660nm</sub>
= 97.0%) of juice was attained after 2 h of incubation at 50 °C in presence of 10 mM CaCl<sub>2</sub>
. Total phenolic content of juice was reduced by 19.8% after clarification, yet with slightly higher %DPPH radical scavenging property.</p>
</abstract>
<kwd-group><kwd>polygalacturonase</kwd>
<kwd>α-amylase</kwd>
<kwd>activated charcoal</kwd>
<kwd>decolourization</kwd>
<kwd>apple juice clarification</kwd>
</kwd-group>
<counts><fig-count count="3"></fig-count>
<table-count count="2"></table-count>
<ref-count count="37"></ref-count>
<page-count count="8"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Inde</li>
</country>
</list>
<tree><country name="Inde"><noRegion><name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
</noRegion>
<name sortKey="Banerjee, Rintu" sort="Banerjee, Rintu" uniqKey="Banerjee R" first="Rintu" last="Banerjee">Rintu Banerjee</name>
<name sortKey="Dey, Tapati Bhanja" sort="Dey, Tapati Bhanja" uniqKey="Dey T" first="Tapati Bhanja" last="Dey">Tapati Bhanja Dey</name>
</country>
</tree>
</affiliations>
</record>
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