Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).
Identifieur interne : 000036 ( Ncbi/Checkpoint ); précédent : 000035; suivant : 000037Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).
Auteurs : C. Betz [États-Unis] ; T G Mccollum ; R T MayerSource :
- Plant molecular biology [ 0167-4412 ] ; 2001.
English descriptors
- KwdEn :
- Citrus (enzymology), Cytochrome P-450 Enzyme System (genetics), DNA, Complementary, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Mixed Function Oxygenases (genetics), Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Trans-Cinnamate 4-Monooxygenase.
- MESH :
- chemical , genetics : Cytochrome P-450 Enzyme System, Mixed Function Oxygenases.
- enzymology : Citrus.
- chemical : DNA, Complementary, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Trans-Cinnamate 4-Monooxygenase.
Abstract
Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.
PubMed: 11575728
Affiliations:
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pubmed:11575728Le document en format XML
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Betz</name><affiliation wicri:level="2"><nlm:affiliation>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T G" last="Mccollum">T G Mccollum</name></author><author><name sortKey="Mayer, R T" sort="Mayer, R T" uniqKey="Mayer R" first="R T" last="Mayer">R T Mayer</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11575728</idno><idno type="pmid">11575728</idno><idno type="wicri:Area/PubMed/Corpus">000E91</idno><idno type="wicri:Area/PubMed/Curation">000E91</idno><idno type="wicri:Area/PubMed/Checkpoint">000E91</idno><idno type="wicri:Area/Ncbi/Merge">000036</idno><idno type="wicri:Area/Ncbi/Curation">000036</idno><idno type="wicri:Area/Ncbi/Checkpoint">000036</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).</title><author><name sortKey="Betz, C" sort="Betz, C" uniqKey="Betz C" first="C" last="Betz">C. Betz</name><affiliation wicri:level="2"><nlm:affiliation>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T G" last="Mccollum">T G Mccollum</name></author><author><name sortKey="Mayer, R T" sort="Mayer, R T" uniqKey="Mayer R" first="R T" last="Mayer">R T Mayer</name></author></analytic><series><title level="j">Plant molecular biology</title><idno type="ISSN">0167-4412</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (enzymology)</term><term>Cytochrome P-450 Enzyme System (genetics)</term><term>DNA, Complementary</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Mixed Function Oxygenases (genetics)</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Trans-Cinnamate 4-Monooxygenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytochrome P-450 Enzyme System</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Trans-Cinnamate 4-Monooxygenase</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><noCountry><name sortKey="Mayer, R T" sort="Mayer, R T" uniqKey="Mayer R" first="R T" last="Mayer">R T Mayer</name><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T G" last="Mccollum">T G Mccollum</name></noCountry><country name="États-Unis"><region name="Floride"><name sortKey="Betz, C" sort="Betz, C" uniqKey="Betz C" first="C" last="Betz">C. Betz</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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