Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).
Identifieur interne : 000E91 ( PubMed/Corpus ); précédent : 000E90; suivant : 000E92Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).
Auteurs : C. Betz ; T G Mccollum ; R T MayerSource :
- Plant molecular biology [ 0167-4412 ] ; 2001.
English descriptors
- KwdEn :
- Citrus (enzymology), Cytochrome P-450 Enzyme System (genetics), DNA, Complementary, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Mixed Function Oxygenases (genetics), Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Trans-Cinnamate 4-Monooxygenase.
- MESH :
- chemical , genetics : Cytochrome P-450 Enzyme System, Mixed Function Oxygenases.
- enzymology : Citrus.
- chemical : DNA, Complementary, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Trans-Cinnamate 4-Monooxygenase.
Abstract
Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.
PubMed: 11575728
Links to Exploration step
pubmed:11575728Le document en format XML
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<author><name sortKey="Betz, C" sort="Betz, C" uniqKey="Betz C" first="C" last="Betz">C. Betz</name>
<affiliation><nlm:affiliation>USDA, ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.</nlm:affiliation>
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<author><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T G" last="Mccollum">T G Mccollum</name>
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<author><name sortKey="Mayer, R T" sort="Mayer, R T" uniqKey="Mayer R" first="R T" last="Mayer">R T Mayer</name>
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<series><title level="j">Plant molecular biology</title>
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<term>Cytochrome P-450 Enzyme System (genetics)</term>
<term>DNA, Complementary</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Gene Expression Regulation, Plant</term>
<term>Mixed Function Oxygenases (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Trans-Cinnamate 4-Monooxygenase</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytochrome P-450 Enzyme System</term>
<term>Mixed Function Oxygenases</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Gene Expression Regulation, Plant</term>
<term>Molecular Sequence Data</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Trans-Cinnamate 4-Monooxygenase</term>
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<front><div type="abstract" xml:lang="en">Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.</div>
</front>
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<DateCreated><Year>2001</Year>
<Month>9</Month>
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<DateCompleted><Year>2001</Year>
<Month>10</Month>
<Day>18</Day>
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<JournalIssue CitedMedium="Print"><Volume>46</Volume>
<Issue>6</Issue>
<PubDate><Year>2001</Year>
<Month>Aug</Month>
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<Title>Plant molecular biology</Title>
<ISOAbbreviation>Plant Mol. Biol.</ISOAbbreviation>
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<ArticleTitle>Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia' orange (Citrus sinensis Osbeck).</ArticleTitle>
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<Abstract><AbstractText>Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from 'not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.</AbstractText>
</Abstract>
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<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
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<MeshHeading><DescriptorName UI="D050564" MajorTopicYN="N">Trans-Cinnamate 4-Monooxygenase</DescriptorName>
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