Fertile fruit trees obtained by somatic hybridization: navel orange (Citrus sinensis) and Troyer citrange (C. sinensis x Poncirus trifoliata).
Identifieur interne : 000E91 ( PubMed/Checkpoint ); précédent : 000E90; suivant : 000E92Fertile fruit trees obtained by somatic hybridization: navel orange (Citrus sinensis) and Troyer citrange (C. sinensis x Poncirus trifoliata).
Auteurs : T. Ohgawara [Japon] ; S. Kobayashi ; S. Ishii ; K. Yoshinaga ; I. OiyamaSource :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [ 0040-5752 ] ; 1991.
Abstract
Nucellar cell suspension protoplasts of navel orange (Citrus sinsensis Osb.) were chemically fused with mesophyll protoplasts of Troyer citrange (C. sinensis x Poncirus trifoliata) and cultured in hormone-free Murashige and Tucker medium containing 0.6 M sucrose. Two types of plant were regenerated through embryogenesis. One type showed intermediate mono-and difoliate leaves and the other types was identical to Troyer citrange. The regenerated plants with intermediate morphology were demonstrated by chromosome counts and rDNA analysis to be amphidiploid somatic hybrids. Five clones of these somatic hybrids were grafted in the field. After 4 years, they set flowers having a morphology intermediate between those of the two parents. The pollen grains showed high stainability and sufficient germinability, and were larger than those of Troyer citrange. The fruits of the somatic hybrids were large and spherical with thick rinds. Most of them contained seeds with normal germinability. These results indicate that somatic hybridization is a useful tool for Citrus breeding.
DOI: 10.1007/BF00215714
PubMed: 24221194
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Ishii</name></author><author><name sortKey="Yoshinaga, K" sort="Yoshinaga, K" uniqKey="Yoshinaga K" first="K" last="Yoshinaga">K. Yoshinaga</name></author><author><name sortKey="Oiyama, I" sort="Oiyama, I" uniqKey="Oiyama I" first="I" last="Oiyama">I. Oiyama</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:24221194</idno><idno type="pmid">24221194</idno><idno type="doi">10.1007/BF00215714</idno><idno type="wicri:Area/PubMed/Corpus">000F56</idno><idno type="wicri:Area/PubMed/Curation">000F56</idno><idno type="wicri:Area/PubMed/Checkpoint">000F56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fertile fruit trees obtained by somatic hybridization: navel orange (Citrus sinensis) and Troyer citrange (C. sinensis x Poncirus trifoliata).</title><author><name sortKey="Ohgawara, T" sort="Ohgawara, T" uniqKey="Ohgawara T" first="T" last="Ohgawara">T. Ohgawara</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Division, Kikkoman Corporation, 399 Noda, 278, Noda City, Chiba, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Research and Development Division, Kikkoman Corporation, 399 Noda, 278, Noda City, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Kobayashi, S" sort="Kobayashi, S" uniqKey="Kobayashi S" first="S" last="Kobayashi">S. Kobayashi</name></author><author><name sortKey="Ishii, S" sort="Ishii, S" uniqKey="Ishii S" first="S" last="Ishii">S. Ishii</name></author><author><name sortKey="Yoshinaga, K" sort="Yoshinaga, K" uniqKey="Yoshinaga K" first="K" last="Yoshinaga">K. Yoshinaga</name></author><author><name sortKey="Oiyama, I" sort="Oiyama, I" uniqKey="Oiyama I" first="I" last="Oiyama">I. Oiyama</name></author></analytic><series><title level="j">TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</title><idno type="ISSN">0040-5752</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nucellar cell suspension protoplasts of navel orange (Citrus sinsensis Osb.) were chemically fused with mesophyll protoplasts of Troyer citrange (C. sinensis x Poncirus trifoliata) and cultured in hormone-free Murashige and Tucker medium containing 0.6 M sucrose. Two types of plant were regenerated through embryogenesis. One type showed intermediate mono-and difoliate leaves and the other types was identical to Troyer citrange. The regenerated plants with intermediate morphology were demonstrated by chromosome counts and rDNA analysis to be amphidiploid somatic hybrids. Five clones of these somatic hybrids were grafted in the field. After 4 years, they set flowers having a morphology intermediate between those of the two parents. The pollen grains showed high stainability and sufficient germinability, and were larger than those of Troyer citrange. The fruits of the somatic hybrids were large and spherical with thick rinds. Most of them contained seeds with normal germinability. These results indicate that somatic hybridization is a useful tool for Citrus breeding.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24221194</PMID><DateCreated><Year>2013</Year><Month>11</Month><Day>13</Day></DateCreated><DateCompleted><Year>2013</Year><Month>11</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0040-5752</ISSN><JournalIssue CitedMedium="Print"><Volume>81</Volume><Issue>2</Issue><PubDate><Year>1991</Year><Month>Feb</Month></PubDate></JournalIssue><Title>TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</Title><ISOAbbreviation>Theor. Appl. 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Ishii</name><name sortKey="Kobayashi, S" sort="Kobayashi, S" uniqKey="Kobayashi S" first="S" last="Kobayashi">S. Kobayashi</name><name sortKey="Oiyama, I" sort="Oiyama, I" uniqKey="Oiyama I" first="I" last="Oiyama">I. Oiyama</name><name sortKey="Yoshinaga, K" sort="Yoshinaga, K" uniqKey="Yoshinaga K" first="K" last="Yoshinaga">K. Yoshinaga</name></noCountry><country name="Japon"><noRegion><name sortKey="Ohgawara, T" sort="Ohgawara, T" uniqKey="Ohgawara T" first="T" last="Ohgawara">T. Ohgawara</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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