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From Model to Crop: Functional Analysis of a STAY-GREEN Gene in the Model Legume Medicago truncatula and Effective Use of the Gene for Alfalfa Improvement1[W][OA]

Identifieur interne : 001730 ( Main/Exploration ); précédent : 001729; suivant : 001731

From Model to Crop: Functional Analysis of a STAY-GREEN Gene in the Model Legume Medicago truncatula and Effective Use of the Gene for Alfalfa Improvement1[W][OA]

Auteurs : Chuanen Zhou ; Lu Han ; Catalina Pislariu ; Jin Nakashima ; Chunxiang Fu ; Qingzhen Jiang ; Li Quan ; Elison B. Blancaflor ; Yuhong Tang ; Joseph H. Bouton ; Michael Udvardi ; Guangmin Xia ; Zeng-Yu Wang

Source :

Plant Physiology [ 0032-0889 ] ; 2011.

RBID : PMC:3252161

Abstract

Medicago truncatula has been developed into a model legume. Its close relative alfalfa (Medicago sativa) is the most widely grown forage legume crop in the United States. By screening a large population of M. truncatula mutants tagged with the transposable element of tobacco (Nicotiana tabacum) cell type1 (Tnt1), we identified a mutant line (NF2089) that maintained green leaves and showed green anthers, central carpels, mature pods, and seeds during senescence. Genetic and molecular analyses revealed that the mutation was caused by Tnt1 insertion in a STAY-GREEN (MtSGR) gene. Transcript profiling analysis of the mutant showed that loss of the MtSGR function affected the expression of a large number of genes involved in different biological processes. Further analyses revealed that SGR is implicated in nodule development and senescence. MtSGR expression was detected across all nodule developmental zones and was higher in the senescence zone. The number of young nodules on the mutant roots was higher than in the wild type. Expression levels of several nodule senescence markers were reduced in the sgr mutant. Based on the MtSGR sequence, an alfalfa SGR gene (MsSGR) was cloned, and transgenic alfalfa lines were produced by RNA interference. Silencing of MsSGR led to the production of stay-green transgenic alfalfa. This beneficial trait offers the opportunity to produce premium alfalfa hay with a more greenish appearance. In addition, most of the transgenic alfalfa lines retained more than 50% of chlorophylls during senescence and had increased crude protein content. This study illustrates the effective use of knowledge gained from a model system for the genetic improvement of an important commercial crop.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252161

DOI: 10.1104/pp.111.185140
PubMed: 21957014
PubMed Central: 3252161

Affiliations:

Le document en format XML

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 Gene in the Model Legume <italic>Medicago truncatula</italic>
 and Effective Use of the Gene for Alfalfa Improvement<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
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<xref ref-type="author-notes" rid="fn4"><sup>[W]</sup>
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<author><name sortKey="Han, Lu" sort="Han, Lu" uniqKey="Han L" first="Lu" last="Han">Lu Han</name>
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<author><name sortKey="Pislariu, Catalina" sort="Pislariu, Catalina" uniqKey="Pislariu C" first="Catalina" last="Pislariu">Catalina Pislariu</name>
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<author><name sortKey="Jiang, Qingzhen" sort="Jiang, Qingzhen" uniqKey="Jiang Q" first="Qingzhen" last="Jiang">Qingzhen Jiang</name>
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<author><name sortKey="Quan, Li" sort="Quan, Li" uniqKey="Quan L" first="Li" last="Quan">Li Quan</name>
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<author><name sortKey="Blancaflor, Elison B" sort="Blancaflor, Elison B" uniqKey="Blancaflor E" first="Elison B." last="Blancaflor">Elison B. Blancaflor</name>
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<author><name sortKey="Tang, Yuhong" sort="Tang, Yuhong" uniqKey="Tang Y" first="Yuhong" last="Tang">Yuhong Tang</name>
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<author><name sortKey="Bouton, Joseph H" sort="Bouton, Joseph H" uniqKey="Bouton J" first="Joseph H." last="Bouton">Joseph H. Bouton</name>
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 Gene in the Model Legume <italic>Medicago truncatula</italic>
 and Effective Use of the Gene for Alfalfa Improvement<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
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<xref ref-type="author-notes" rid="fn4"><sup>[W]</sup>
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<xref ref-type="author-notes" rid="fn5"><sup>[OA]</sup>
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<author><name sortKey="Zhou, Chuanen" sort="Zhou, Chuanen" uniqKey="Zhou C" first="Chuanen" last="Zhou">Chuanen Zhou</name>
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<author><name sortKey="Han, Lu" sort="Han, Lu" uniqKey="Han L" first="Lu" last="Han">Lu Han</name>
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<author><name sortKey="Pislariu, Catalina" sort="Pislariu, Catalina" uniqKey="Pislariu C" first="Catalina" last="Pislariu">Catalina Pislariu</name>
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<author><name sortKey="Nakashima, Jin" sort="Nakashima, Jin" uniqKey="Nakashima J" first="Jin" last="Nakashima">Jin Nakashima</name>
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<author><name sortKey="Fu, Chunxiang" sort="Fu, Chunxiang" uniqKey="Fu C" first="Chunxiang" last="Fu">Chunxiang Fu</name>
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<author><name sortKey="Jiang, Qingzhen" sort="Jiang, Qingzhen" uniqKey="Jiang Q" first="Qingzhen" last="Jiang">Qingzhen Jiang</name>
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<author><name sortKey="Bouton, Joseph H" sort="Bouton, Joseph H" uniqKey="Bouton J" first="Joseph H." last="Bouton">Joseph H. Bouton</name>
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<author><name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name>
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<front><div type="abstract" xml:lang="en"><p><italic>Medicago truncatula</italic>
 has been developed into a model legume. Its close relative alfalfa (<italic>Medicago sativa</italic>
) is the most widely grown forage legume crop in the United States. By screening a large population of <italic>M. truncatula</italic>
 mutants tagged with the transposable element of tobacco (<italic>Nicotiana tabacum</italic>
) cell type1 (<italic>Tnt1</italic>
), we identified a mutant line (NF2089) that maintained green leaves and showed green anthers, central carpels, mature pods, and seeds during senescence. Genetic and molecular analyses revealed that the mutation was caused by <italic>Tnt1</italic>
 insertion in a <italic>STAY-GREEN</italic>
 (<italic>MtSGR</italic>
) gene. Transcript profiling analysis of the mutant showed that loss of the <italic>MtSGR</italic>
 function affected the expression of a large number of genes involved in different biological processes. Further analyses revealed that SGR is implicated in nodule development and senescence. <italic>MtSGR</italic>
 expression was detected across all nodule developmental zones and was higher in the senescence zone. The number of young nodules on the mutant roots was higher than in the wild type. Expression levels of several nodule senescence markers were reduced in the <italic>sgr</italic>
 mutant. Based on the <italic>MtSGR</italic>
 sequence, an alfalfa <italic>SGR</italic>
 gene (<italic>MsSGR</italic>
) was cloned, and transgenic alfalfa lines were produced by RNA interference. Silencing of <italic>MsSGR</italic>
 led to the production of stay-green transgenic alfalfa. This beneficial trait offers the opportunity to produce premium alfalfa hay with a more greenish appearance. In addition, most of the transgenic alfalfa lines retained more than 50% of chlorophylls during senescence and had increased crude protein content. This study illustrates the effective use of knowledge gained from a model system for the genetic improvement of an important commercial crop.</p>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Blancaflor, Elison B" sort="Blancaflor, Elison B" uniqKey="Blancaflor E" first="Elison B." last="Blancaflor">Elison B. Blancaflor</name>
<name sortKey="Bouton, Joseph H" sort="Bouton, Joseph H" uniqKey="Bouton J" first="Joseph H." last="Bouton">Joseph H. Bouton</name>
<name sortKey="Fu, Chunxiang" sort="Fu, Chunxiang" uniqKey="Fu C" first="Chunxiang" last="Fu">Chunxiang Fu</name>
<name sortKey="Han, Lu" sort="Han, Lu" uniqKey="Han L" first="Lu" last="Han">Lu Han</name>
<name sortKey="Jiang, Qingzhen" sort="Jiang, Qingzhen" uniqKey="Jiang Q" first="Qingzhen" last="Jiang">Qingzhen Jiang</name>
<name sortKey="Nakashima, Jin" sort="Nakashima, Jin" uniqKey="Nakashima J" first="Jin" last="Nakashima">Jin Nakashima</name>
<name sortKey="Pislariu, Catalina" sort="Pislariu, Catalina" uniqKey="Pislariu C" first="Catalina" last="Pislariu">Catalina Pislariu</name>
<name sortKey="Quan, Li" sort="Quan, Li" uniqKey="Quan L" first="Li" last="Quan">Li Quan</name>
<name sortKey="Tang, Yuhong" sort="Tang, Yuhong" uniqKey="Tang Y" first="Yuhong" last="Tang">Yuhong Tang</name>
<name sortKey="Udvardi, Michael" sort="Udvardi, Michael" uniqKey="Udvardi M" first="Michael" last="Udvardi">Michael Udvardi</name>
<name sortKey="Wang, Zeng Yu" sort="Wang, Zeng Yu" uniqKey="Wang Z" first="Zeng-Yu" last="Wang">Zeng-Yu Wang</name>
<name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name>
<name sortKey="Zhou, Chuanen" sort="Zhou, Chuanen" uniqKey="Zhou C" first="Chuanen" last="Zhou">Chuanen Zhou</name>
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From Model to Crop: Functional Analysis of a STAY-GREEN Gene in the Model Legume Medicago truncatula and Effective Use of the Gene for Alfalfa Improvement1[W][OA]

From Model to Crop: Functional Analysis of a STAY-GREEN Gene in the Model Legume Medicago truncatula and Effective Use of the Gene for Alfalfa Improvement1[W][OA]

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Abstract

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