From Model to Crop: Functional Analysis of a STAY-GREEN Gene in the Model Legume Medicago truncatula and Effective Use of the Gene for Alfalfa Improvement1[W][OA]
Identifieur interne : 000E56 ( Pmc/Checkpoint ); précédent : 000E55; suivant : 000E57From Model to Crop: Functional Analysis of a STAY-GREEN Gene in the Model Legume Medicago truncatula and Effective Use of the Gene for Alfalfa Improvement1[W][OA]
Auteurs : Chuanen Zhou ; Lu Han ; Catalina Pislariu ; Jin Nakashima ; Chunxiang Fu ; Qingzhen Jiang ; Li Quan ; Elison B. Blancaflor ; Yuhong Tang ; Joseph H. Bouton ; Michael Udvardi ; Guangmin Xia ; Zeng-Yu WangSource :
- Plant Physiology [ 0032-0889 ] ; 2011.
Abstract
Url:
DOI: 10.1104/pp.111.185140
PubMed: 21957014
PubMed Central: 3252161
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">From Model to Crop: Functional Analysis of a <italic>STAY-GREEN</italic> Gene in the Model Legume <italic>Medicago truncatula</italic> and Effective Use of the Gene for Alfalfa Improvement<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn4"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn5"><sup>[OA]</sup></xref></title><author><name sortKey="Zhou, Chuanen" sort="Zhou, Chuanen" uniqKey="Zhou C" first="Chuanen" last="Zhou">Chuanen Zhou</name></author><author><name sortKey="Han, Lu" sort="Han, Lu" uniqKey="Han L" first="Lu" last="Han">Lu Han</name></author><author><name sortKey="Pislariu, Catalina" sort="Pislariu, Catalina" uniqKey="Pislariu C" first="Catalina" last="Pislariu">Catalina Pislariu</name></author><author><name sortKey="Nakashima, Jin" sort="Nakashima, Jin" uniqKey="Nakashima J" first="Jin" last="Nakashima">Jin Nakashima</name></author><author><name sortKey="Fu, Chunxiang" sort="Fu, Chunxiang" uniqKey="Fu C" first="Chunxiang" last="Fu">Chunxiang Fu</name></author><author><name sortKey="Jiang, Qingzhen" sort="Jiang, Qingzhen" uniqKey="Jiang Q" first="Qingzhen" last="Jiang">Qingzhen Jiang</name></author><author><name sortKey="Quan, Li" sort="Quan, Li" uniqKey="Quan L" first="Li" last="Quan">Li Quan</name></author><author><name sortKey="Blancaflor, Elison B" sort="Blancaflor, Elison B" uniqKey="Blancaflor E" first="Elison B." last="Blancaflor">Elison B. Blancaflor</name></author><author><name sortKey="Tang, Yuhong" sort="Tang, Yuhong" uniqKey="Tang Y" first="Yuhong" last="Tang">Yuhong Tang</name></author><author><name sortKey="Bouton, Joseph H" sort="Bouton, Joseph H" uniqKey="Bouton J" first="Joseph H." last="Bouton">Joseph H. Bouton</name></author><author><name sortKey="Udvardi, Michael" sort="Udvardi, Michael" uniqKey="Udvardi M" first="Michael" last="Udvardi">Michael Udvardi</name></author><author><name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name></author><author><name sortKey="Wang, Zeng Yu" sort="Wang, Zeng Yu" uniqKey="Wang Z" first="Zeng-Yu" last="Wang">Zeng-Yu Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21957014</idno><idno type="pmc">3252161</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252161</idno><idno type="RBID">PMC:3252161</idno><idno type="doi">10.1104/pp.111.185140</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000C95</idno><idno type="wicri:Area/Pmc/Curation">000C94</idno><idno type="wicri:Area/Pmc/Checkpoint">000E56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">From Model to Crop: Functional Analysis of a <italic>STAY-GREEN</italic> Gene in the Model Legume <italic>Medicago truncatula</italic> and Effective Use of the Gene for Alfalfa Improvement<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn4"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn5"><sup>[OA]</sup></xref></title><author><name sortKey="Zhou, Chuanen" sort="Zhou, Chuanen" uniqKey="Zhou C" first="Chuanen" last="Zhou">Chuanen Zhou</name></author><author><name sortKey="Han, Lu" sort="Han, Lu" uniqKey="Han L" first="Lu" last="Han">Lu Han</name></author><author><name sortKey="Pislariu, Catalina" sort="Pislariu, Catalina" uniqKey="Pislariu C" first="Catalina" last="Pislariu">Catalina Pislariu</name></author><author><name sortKey="Nakashima, Jin" sort="Nakashima, Jin" uniqKey="Nakashima J" first="Jin" last="Nakashima">Jin Nakashima</name></author><author><name sortKey="Fu, Chunxiang" sort="Fu, Chunxiang" uniqKey="Fu C" first="Chunxiang" last="Fu">Chunxiang Fu</name></author><author><name sortKey="Jiang, Qingzhen" sort="Jiang, Qingzhen" uniqKey="Jiang Q" first="Qingzhen" last="Jiang">Qingzhen Jiang</name></author><author><name sortKey="Quan, Li" sort="Quan, Li" uniqKey="Quan L" first="Li" last="Quan">Li Quan</name></author><author><name sortKey="Blancaflor, Elison B" sort="Blancaflor, Elison B" uniqKey="Blancaflor E" first="Elison B." last="Blancaflor">Elison B. Blancaflor</name></author><author><name sortKey="Tang, Yuhong" sort="Tang, Yuhong" uniqKey="Tang Y" first="Yuhong" last="Tang">Yuhong Tang</name></author><author><name sortKey="Bouton, Joseph H" sort="Bouton, Joseph H" uniqKey="Bouton J" first="Joseph H." last="Bouton">Joseph H. Bouton</name></author><author><name sortKey="Udvardi, Michael" sort="Udvardi, Michael" uniqKey="Udvardi M" first="Michael" last="Udvardi">Michael Udvardi</name></author><author><name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name></author><author><name sortKey="Wang, Zeng Yu" sort="Wang, Zeng Yu" uniqKey="Wang Z" first="Zeng-Yu" last="Wang">Zeng-Yu Wang</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Medicago truncatula</italic> has been developed into a model legume. Its close relative alfalfa (<italic>Medicago sativa</italic>) is the most widely grown forage legume crop in the United States. By screening a large population of <italic>M. truncatula</italic> mutants tagged with the transposable element of tobacco (<italic>Nicotiana tabacum</italic>) cell type1 (<italic>Tnt1</italic>), we identified a mutant line (NF2089) that maintained green leaves and showed green anthers, central carpels, mature pods, and seeds during senescence. Genetic and molecular analyses revealed that the mutation was caused by <italic>Tnt1</italic> insertion in a <italic>STAY-GREEN</italic> (<italic>MtSGR</italic>) gene. Transcript profiling analysis of the mutant showed that loss of the <italic>MtSGR</italic> function affected the expression of a large number of genes involved in different biological processes. Further analyses revealed that SGR is implicated in nodule development and senescence. <italic>MtSGR</italic> expression was detected across all nodule developmental zones and was higher in the senescence zone. The number of young nodules on the mutant roots was higher than in the wild type. Expression levels of several nodule senescence markers were reduced in the <italic>sgr</italic> mutant. Based on the <italic>MtSGR</italic> sequence, an alfalfa <italic>SGR</italic> gene (<italic>MsSGR</italic>) was cloned, and transgenic alfalfa lines were produced by RNA interference. Silencing of <italic>MsSGR</italic> led to the production of stay-green transgenic alfalfa. This beneficial trait offers the opportunity to produce premium alfalfa hay with a more greenish appearance. In addition, most of the transgenic alfalfa lines retained more than 50% of chlorophylls during senescence and had increased crude protein content. This study illustrates the effective use of knowledge gained from a model system for the genetic improvement of an important commercial crop.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="hwp">plantphysiol</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>Plant Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21957014</article-id><article-id pub-id-type="pmc">3252161</article-id><article-id pub-id-type="publisher-id">185140</article-id><article-id pub-id-type="doi">10.1104/pp.111.185140</article-id><article-categories><subj-group subj-group-type="heading"><subject>Systems Biology, Molecular Biology, and Gene Regulation</subject></subj-group></article-categories><title-group><article-title>From Model to Crop: Functional Analysis of a <italic>STAY-GREEN</italic> Gene in the Model Legume <italic>Medicago truncatula</italic> and Effective Use of the Gene for Alfalfa Improvement<xref ref-type="author-notes" rid="fn1"><sup>1</sup></xref><xref ref-type="author-notes" rid="fn4"><sup>[W]</sup></xref><xref ref-type="author-notes" rid="fn5"><sup>[OA]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Chuanen</given-names></name><xref ref-type="author-notes" rid="fn2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Han</surname><given-names>Lu</given-names></name><xref ref-type="author-notes" rid="fn2"><sup>2</sup></xref><xref ref-type="author-notes" rid="fn3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pislariu</surname><given-names>Catalina</given-names></name></contrib><contrib contrib-type="author"><name><surname>Nakashima</surname><given-names>Jin</given-names></name></contrib><contrib contrib-type="author"><name><surname>Fu</surname><given-names>Chunxiang</given-names></name></contrib><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Qingzhen</given-names></name></contrib><contrib contrib-type="author"><name><surname>Quan</surname><given-names>Li</given-names></name></contrib><contrib contrib-type="author"><name><surname>Blancaflor</surname><given-names>Elison B.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Yuhong</given-names></name></contrib><contrib contrib-type="author"><name><surname>Bouton</surname><given-names>Joseph H.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Udvardi</surname><given-names>Michael</given-names></name></contrib><contrib contrib-type="author"><name><surname>Xia</surname><given-names>Guangmin</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Zeng-Yu</given-names></name><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff>Forage Improvement Division (C.Z., L.H., C.F., Q.J., J.H.B., Z.-Y.W.) and Plant Biology Division (C.P., J.N., L.Q., E.B.B., Y.T., M.U.), Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401; Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan 250100, China (L.H., G.X.)</aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author; e-mail <email>zywang@noble.org</email>.</corresp><fn id="fn1"><label>1</label><p>This work was supported by the Samuel Roberts Noble Foundation.</p></fn><fn id="fn2" fn-type="equal"><label>2</label><p>These authors contributed equally to the article.</p></fn><fn id="fn3"><label>3</label><p>Present address: School of Medical and Life Science, University of Jinan, Jinan 250022, China.</p></fn><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Zeng-Yu Wang (<email>zywang@noble.org</email>).</p></fn><fn id="fn4"><label>[W]</label><p>The online version of this article contains Web-only data.</p></fn><fn id="fn5"><label>[OA]</label><p>Open Access articles can be viewed online without a subscription.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org/cgi/doi/10.1104/pp.111.185140">www.plantphysiol.org/cgi/doi/10.1104/pp.111.185140</ext-link></p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>11</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>28</day><month>9</month><year>2011</year></pub-date><pub-date pub-type="pmc-release"><day>28</day><month>9</month><year>2011</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>157</volume><issue>3</issue><fpage>1483</fpage><lpage>1496</lpage><history><date date-type="received"><day>8</day><month>8</month><year>2011</year></date><date date-type="accepted"><day>27</day><month>9</month><year>2011</year></date></history><permissions><copyright-statement>© 2011 American Society of Plant Biologists. All rights reserved.</copyright-statement><copyright-year>2011</copyright-year></permissions><abstract><p><italic>Medicago truncatula</italic> has been developed into a model legume. Its close relative alfalfa (<italic>Medicago sativa</italic>) is the most widely grown forage legume crop in the United States. By screening a large population of <italic>M. truncatula</italic> mutants tagged with the transposable element of tobacco (<italic>Nicotiana tabacum</italic>) cell type1 (<italic>Tnt1</italic>), we identified a mutant line (NF2089) that maintained green leaves and showed green anthers, central carpels, mature pods, and seeds during senescence. Genetic and molecular analyses revealed that the mutation was caused by <italic>Tnt1</italic> insertion in a <italic>STAY-GREEN</italic> (<italic>MtSGR</italic>) gene. Transcript profiling analysis of the mutant showed that loss of the <italic>MtSGR</italic> function affected the expression of a large number of genes involved in different biological processes. Further analyses revealed that SGR is implicated in nodule development and senescence. <italic>MtSGR</italic> expression was detected across all nodule developmental zones and was higher in the senescence zone. The number of young nodules on the mutant roots was higher than in the wild type. Expression levels of several nodule senescence markers were reduced in the <italic>sgr</italic> mutant. Based on the <italic>MtSGR</italic> sequence, an alfalfa <italic>SGR</italic> gene (<italic>MsSGR</italic>) was cloned, and transgenic alfalfa lines were produced by RNA interference. Silencing of <italic>MsSGR</italic> led to the production of stay-green transgenic alfalfa. This beneficial trait offers the opportunity to produce premium alfalfa hay with a more greenish appearance. In addition, most of the transgenic alfalfa lines retained more than 50% of chlorophylls during senescence and had increased crude protein content. This study illustrates the effective use of knowledge gained from a model system for the genetic improvement of an important commercial crop.</p></abstract></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Blancaflor, Elison B" sort="Blancaflor, Elison B" uniqKey="Blancaflor E" first="Elison B." last="Blancaflor">Elison B. Blancaflor</name><name sortKey="Bouton, Joseph H" sort="Bouton, Joseph H" uniqKey="Bouton J" first="Joseph H." last="Bouton">Joseph H. Bouton</name><name sortKey="Fu, Chunxiang" sort="Fu, Chunxiang" uniqKey="Fu C" first="Chunxiang" last="Fu">Chunxiang Fu</name><name sortKey="Han, Lu" sort="Han, Lu" uniqKey="Han L" first="Lu" last="Han">Lu Han</name><name sortKey="Jiang, Qingzhen" sort="Jiang, Qingzhen" uniqKey="Jiang Q" first="Qingzhen" last="Jiang">Qingzhen Jiang</name><name sortKey="Nakashima, Jin" sort="Nakashima, Jin" uniqKey="Nakashima J" first="Jin" last="Nakashima">Jin Nakashima</name><name sortKey="Pislariu, Catalina" sort="Pislariu, Catalina" uniqKey="Pislariu C" first="Catalina" last="Pislariu">Catalina Pislariu</name><name sortKey="Quan, Li" sort="Quan, Li" uniqKey="Quan L" first="Li" last="Quan">Li Quan</name><name sortKey="Tang, Yuhong" sort="Tang, Yuhong" uniqKey="Tang Y" first="Yuhong" last="Tang">Yuhong Tang</name><name sortKey="Udvardi, Michael" sort="Udvardi, Michael" uniqKey="Udvardi M" first="Michael" last="Udvardi">Michael Udvardi</name><name sortKey="Wang, Zeng Yu" sort="Wang, Zeng Yu" uniqKey="Wang Z" first="Zeng-Yu" last="Wang">Zeng-Yu Wang</name><name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name><name sortKey="Zhou, Chuanen" sort="Zhou, Chuanen" uniqKey="Zhou C" first="Chuanen" last="Zhou">Chuanen Zhou</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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