Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck
Identifieur interne : 002E14 ( Main/Exploration ); précédent : 002E13; suivant : 002E15Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck
Auteurs : Gozal Ben-Hayyim [Israël] ; Yardena Gueta-Dahan [Israël] ; Orna Avsian-Kretchmer [Israël] ; Heiko Weichert [Allemagne] ; Ivo Feussner [Allemagne]Source :
- Planta [ 0032-0935 ] ; 2001.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- Abscisic Acid (pharmacology), Antioxidant, Antioxidants (pharmacology), Blotting, Western, Cells, Cultured, Chromatography, High Pressure Liquid, Citrus (enzymology), Citrus sinensis, Defense mechanism, Enzyme Induction (drug effects), Gene expression, Herbicides, Induction, Intraspecific comparison, Lipoxygenase, Lipoxygenase (analysis), Lipoxygenase (biosynthesis), Oxidative Stress (physiology), Oxidative stress, Paraquat (metabolism), Peroxides (metabolism), Plant Growth Regulators (pharmacology), Salinity, Salt resistance, Sodium Chloride (metabolism), Sodium Chloride (pharmacology).
- MESH :
- chemical , analysis : Lipoxygenase.
- chemical , biosynthesis : Lipoxygenase.
- chemical , metabolism : Paraquat, Peroxides, Sodium Chloride.
- chemical , pharmacology : Abscisic Acid, Antioxidants, Plant Growth Regulators, Sodium Chloride.
- drug effects : Enzyme Induction.
- enzymology : Citrus.
- physiology : Oxidative Stress.
- Blotting, Western, Cells, Cultured, Chromatography, High Pressure Liquid, Herbicides.
Abstract
Recent findings in our laboratory suggested that in citrus cells the salt induction of phospholipid hydroperoxide glutathione peroxidase, an enzyme active in cellular antioxidant defense, is mediated by the accumulation of hydroperoxides. Production of hydroperoxides occurs as a result of non-enzymatic autooxidation or via the action of lipoxygenases (LOXs). In an attempt to resolve the role of LOX activity in the accumulation of peroxides we analyzed the expression of this protein under stress conditions and in cells of Citrus sinensis L. differing in sensitivity to salt. Lipoxygenase expression was induced very rapidly only in the salt-tolerant cells and in a transient manner. The induction was specific to salt stress and did not occur with other osmotic-stress-inducing agents, such as polyethylene glycol or mannitol, or under hot or cold conditions, or in the presence of abscisic acid. The induction was eliminated by the antioxidants dithiothreitol and kaempferol, thus once more establishing a correlation between salt and oxidative stresses. Analyses of both in vitro and in vivo products of LOX revealed a specific 9-LOX activity, and a very fast reduction of the hydroperoxides to the corresponding hydroxy derivatives. This suggests that one of the metabolites further downstream in the reductase pathway may play a key role in triggering defense responses against salt stress.
Affiliations:
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Osbeck</title><author><name sortKey="Ben Hayyim, Gozal" sort="Ben Hayyim, Gozal" uniqKey="Ben Hayyim G" first="Gozal" last="Ben-Hayyim">Gozal Ben-Hayyim</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics, ARO, The Volcani Center, P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Gueta Dahan, Yardena" sort="Gueta Dahan, Yardena" uniqKey="Gueta Dahan Y" first="Yardena" last="Gueta-Dahan">Yardena Gueta-Dahan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics, ARO, The Volcani Center, P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Avsian Kretchmer, Orna" sort="Avsian Kretchmer, Orna" uniqKey="Avsian Kretchmer O" first="Orna" last="Avsian-Kretchmer">Orna Avsian-Kretchmer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Fruit-Tree Breeding and Molecular Genetics, ARO, The Volcani Center, P.O. Box 6</s1><s2>Bet Dagan, 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan, 50250</wicri:noRegion></affiliation></author><author><name sortKey="Weichert, Heiko" sort="Weichert, Heiko" uniqKey="Weichert H" first="Heiko" last="Weichert">Heiko Weichert</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Plant Genetics and Crop Plant Research</s1><s2>06466 Gatersleben</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>06466 Gatersleben</wicri:noRegion><wicri:noRegion>Institute of Plant Genetics and Crop Plant Research</wicri:noRegion><wicri:noRegion>Institute of Plant Genetics and Crop Plant Research</wicri:noRegion></affiliation></author><author><name sortKey="Feussner, Ivo" sort="Feussner, Ivo" uniqKey="Feussner I" first="Ivo" last="Feussner">Ivo Feussner</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Plant Genetics and Crop Plant Research</s1><s2>06466 Gatersleben</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>06466 Gatersleben</wicri:noRegion><wicri:noRegion>Institute of Plant Genetics and Crop Plant Research</wicri:noRegion><wicri:noRegion>Institute of Plant Genetics and Crop Plant Research</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Planta</title><title level="j" type="abbreviated">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Planta</title><title level="j" type="abbreviated">Planta</title><idno type="ISSN">0032-0935</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (pharmacology)</term><term>Antioxidant</term><term>Antioxidants (pharmacology)</term><term>Blotting, Western</term><term>Cells, Cultured</term><term>Chromatography, High Pressure Liquid</term><term>Citrus (enzymology)</term><term>Citrus sinensis</term><term>Defense mechanism</term><term>Enzyme Induction (drug effects)</term><term>Gene expression</term><term>Herbicides</term><term>Induction</term><term>Intraspecific comparison</term><term>Lipoxygenase</term><term>Lipoxygenase (analysis)</term><term>Lipoxygenase (biosynthesis)</term><term>Oxidative Stress (physiology)</term><term>Oxidative stress</term><term>Paraquat (metabolism)</term><term>Peroxides (metabolism)</term><term>Plant Growth Regulators (pharmacology)</term><term>Salinity</term><term>Salt resistance</term><term>Sodium Chloride (metabolism)</term><term>Sodium Chloride (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lipoxygenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lipoxygenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Paraquat</term><term>Peroxides</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Abscisic Acid</term><term>Antioxidants</term><term>Plant Growth Regulators</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Enzyme Induction</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Oxidative Stress</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Cells, Cultured</term><term>Chromatography, High Pressure Liquid</term><term>Herbicides</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Salinité</term><term>Résistance sel</term><term>Comparaison intraspécifique</term><term>Induction</term><term>Expression génique</term><term>Lipoxygenase</term><term>Mécanisme défense</term><term>Antioxydant</term><term>Stress oxydatif</term><term>Citrus sinensis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent findings in our laboratory suggested that in citrus cells the salt induction of phospholipid hydroperoxide glutathione peroxidase, an enzyme active in cellular antioxidant defense, is mediated by the accumulation of hydroperoxides. Production of hydroperoxides occurs as a result of non-enzymatic autooxidation or via the action of lipoxygenases (LOXs). In an attempt to resolve the role of LOX activity in the accumulation of peroxides we analyzed the expression of this protein under stress conditions and in cells of Citrus sinensis L. differing in sensitivity to salt. Lipoxygenase expression was induced very rapidly only in the salt-tolerant cells and in a transient manner. The induction was specific to salt stress and did not occur with other osmotic-stress-inducing agents, such as polyethylene glycol or mannitol, or under hot or cold conditions, or in the presence of abscisic acid. The induction was eliminated by the antioxidants dithiothreitol and kaempferol, thus once more establishing a correlation between salt and oxidative stresses. Analyses of both in vitro and in vivo products of LOX revealed a specific 9-LOX activity, and a very fast reduction of the hydroperoxides to the corresponding hydroxy derivatives. This suggests that one of the metabolites further downstream in the reductase pathway may play a key role in triggering defense responses against salt stress.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Israël</li></country></list><tree><country name="Israël"><noRegion><name sortKey="Ben Hayyim, Gozal" sort="Ben Hayyim, Gozal" uniqKey="Ben Hayyim G" first="Gozal" last="Ben-Hayyim">Gozal Ben-Hayyim</name></noRegion><name sortKey="Avsian Kretchmer, Orna" sort="Avsian Kretchmer, Orna" uniqKey="Avsian Kretchmer O" first="Orna" last="Avsian-Kretchmer">Orna Avsian-Kretchmer</name><name sortKey="Gueta Dahan, Yardena" sort="Gueta Dahan, Yardena" uniqKey="Gueta Dahan Y" first="Yardena" last="Gueta-Dahan">Yardena Gueta-Dahan</name></country><country name="Allemagne"><noRegion><name sortKey="Weichert, Heiko" sort="Weichert, Heiko" uniqKey="Weichert H" first="Heiko" last="Weichert">Heiko Weichert</name></noRegion><name sortKey="Feussner, Ivo" sort="Feussner, Ivo" uniqKey="Feussner I" first="Ivo" last="Feussner">Ivo Feussner</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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