Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck.
Identifieur interne : 000E98 ( PubMed/Corpus ); précédent : 000E97; suivant : 000E99Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck.
Auteurs : G. Ben-Hayyim ; Y. Gueta-Dahan ; O. Avsian-Kretchmer ; H. Weichert ; I. FeussnerSource :
- Planta [ 0032-0935 ] ; 2001.
English descriptors
- KwdEn :
- Abscisic Acid (pharmacology), Antioxidants (pharmacology), Blotting, Western, Cells, Cultured, Chromatography, High Pressure Liquid, Citrus (enzymology), Enzyme Induction (drug effects), Herbicides, Lipoxygenase (analysis), Lipoxygenase (biosynthesis), Oxidative Stress (physiology), Paraquat (metabolism), Peroxides (metabolism), Plant Growth Regulators (pharmacology), Sodium Chloride (metabolism), Sodium Chloride (pharmacology).
- MESH :
- chemical , analysis : Lipoxygenase.
- chemical , biosynthesis : Lipoxygenase.
- chemical , metabolism : Paraquat, Peroxides, Sodium Chloride.
- chemical , pharmacology : Abscisic Acid, Antioxidants, Plant Growth Regulators, Sodium Chloride.
- drug effects : Enzyme Induction.
- enzymology : Citrus.
- physiology : Oxidative Stress.
- Blotting, Western, Cells, Cultured, Chromatography, High Pressure Liquid, Herbicides.
Abstract
Recent findings in our laboratory suggested that in citrus cells the salt induction of phospholipid hydroperoxide glutathione peroxidase, an enzyme active in cellular antioxidant defense, is mediated by the accumulation of hydroperoxides. Production of hydroperoxides occurs as a result of non-enzymatic auto-oxidation or via the action of lipoxygenases (LOXs). In an attempt to resolve the role of LOX activity in the accumulation of peroxides we analyzed the expression of this protein under stress conditions and in cells of Citrus sinensis L. differing in sensitivity to salt. Lipoxygenase expression was induced very rapidly only in the salt-tolerant cells and in a transient manner. The induction was specific to salt stress and did not occur with other osmotic-stress-inducing agents, such as polyethylene glycol or mannitol, or under hot or cold conditions, or in the presence of abscisic acid. The induction was eliminated by the antioxidants dithiothreitol and kaempferol, thus once more establishing a correlation between salt and oxidative stresses. Analyses of both in vitro and in vivo products of LOX revealed a specific 9-LOX activity, and a very fast reduction of the hydroperoxides to the corresponding hydroxy derivatives. This suggests that one of the metabolites further downstream in the reductase pathway may play a key role in triggering defense responses against salt stress.
DOI: 10.1007/s004250000397
PubMed: 11289601
Links to Exploration step
pubmed:11289601Le document en format XML
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Weichert</name></author><author><name sortKey="Feussner, I" sort="Feussner, I" uniqKey="Feussner I" first="I" last="Feussner">I. Feussner</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11289601</idno><idno type="pmid">11289601</idno><idno type="doi">10.1007/s004250000397</idno><idno type="wicri:Area/PubMed/Corpus">000E98</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck.</title><author><name sortKey="Ben Hayyim, G" sort="Ben Hayyim, G" uniqKey="Ben Hayyim G" first="G" last="Ben-Hayyim">G. Ben-Hayyim</name><affiliation><nlm:affiliation>Department of Fruit-Tree Breeding and Molecular Genetics, ARO, The Volcani Center, Bet Dagan, Israel. vhgozal@volcani.agri.gov.il</nlm:affiliation></affiliation></author><author><name sortKey="Gueta Dahan, Y" sort="Gueta Dahan, Y" uniqKey="Gueta Dahan Y" first="Y" last="Gueta-Dahan">Y. Gueta-Dahan</name></author><author><name sortKey="Avsian Kretchmer, O" sort="Avsian Kretchmer, O" uniqKey="Avsian Kretchmer O" first="O" last="Avsian-Kretchmer">O. Avsian-Kretchmer</name></author><author><name sortKey="Weichert, H" sort="Weichert, H" uniqKey="Weichert H" first="H" last="Weichert">H. Weichert</name></author><author><name sortKey="Feussner, I" sort="Feussner, I" uniqKey="Feussner I" first="I" last="Feussner">I. Feussner</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (pharmacology)</term><term>Antioxidants (pharmacology)</term><term>Blotting, Western</term><term>Cells, Cultured</term><term>Chromatography, High Pressure Liquid</term><term>Citrus (enzymology)</term><term>Enzyme Induction (drug effects)</term><term>Herbicides</term><term>Lipoxygenase (analysis)</term><term>Lipoxygenase (biosynthesis)</term><term>Oxidative Stress (physiology)</term><term>Paraquat (metabolism)</term><term>Peroxides (metabolism)</term><term>Plant Growth Regulators (pharmacology)</term><term>Sodium Chloride (metabolism)</term><term>Sodium Chloride (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lipoxygenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lipoxygenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Paraquat</term><term>Peroxides</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Abscisic Acid</term><term>Antioxidants</term><term>Plant Growth Regulators</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Enzyme Induction</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Oxidative Stress</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Cells, Cultured</term><term>Chromatography, High Pressure Liquid</term><term>Herbicides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent findings in our laboratory suggested that in citrus cells the salt induction of phospholipid hydroperoxide glutathione peroxidase, an enzyme active in cellular antioxidant defense, is mediated by the accumulation of hydroperoxides. Production of hydroperoxides occurs as a result of non-enzymatic auto-oxidation or via the action of lipoxygenases (LOXs). In an attempt to resolve the role of LOX activity in the accumulation of peroxides we analyzed the expression of this protein under stress conditions and in cells of Citrus sinensis L. differing in sensitivity to salt. Lipoxygenase expression was induced very rapidly only in the salt-tolerant cells and in a transient manner. The induction was specific to salt stress and did not occur with other osmotic-stress-inducing agents, such as polyethylene glycol or mannitol, or under hot or cold conditions, or in the presence of abscisic acid. The induction was eliminated by the antioxidants dithiothreitol and kaempferol, thus once more establishing a correlation between salt and oxidative stresses. Analyses of both in vitro and in vivo products of LOX revealed a specific 9-LOX activity, and a very fast reduction of the hydroperoxides to the corresponding hydroxy derivatives. This suggests that one of the metabolites further downstream in the reductase pathway may play a key role in triggering defense responses against salt stress.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11289601</PMID><DateCreated><Year>2001</Year><Month>4</Month><Day>5</Day></DateCreated><DateCompleted><Year>2001</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>212</Volume><Issue>3</Issue><PubDate><Year>2001</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck.</ArticleTitle><Pagination><MedlinePgn>367-75</MedlinePgn></Pagination><Abstract><AbstractText>Recent findings in our laboratory suggested that in citrus cells the salt induction of phospholipid hydroperoxide glutathione peroxidase, an enzyme active in cellular antioxidant defense, is mediated by the accumulation of hydroperoxides. Production of hydroperoxides occurs as a result of non-enzymatic auto-oxidation or via the action of lipoxygenases (LOXs). In an attempt to resolve the role of LOX activity in the accumulation of peroxides we analyzed the expression of this protein under stress conditions and in cells of Citrus sinensis L. differing in sensitivity to salt. Lipoxygenase expression was induced very rapidly only in the salt-tolerant cells and in a transient manner. The induction was specific to salt stress and did not occur with other osmotic-stress-inducing agents, such as polyethylene glycol or mannitol, or under hot or cold conditions, or in the presence of abscisic acid. The induction was eliminated by the antioxidants dithiothreitol and kaempferol, thus once more establishing a correlation between salt and oxidative stresses. Analyses of both in vitro and in vivo products of LOX revealed a specific 9-LOX activity, and a very fast reduction of the hydroperoxides to the corresponding hydroxy derivatives. This suggests that one of the metabolites further downstream in the reductase pathway may play a key role in triggering defense responses against salt stress.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ben-Hayyim</LastName><ForeName>G</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Fruit-Tree Breeding and Molecular Genetics, ARO, The Volcani Center, Bet Dagan, Israel. vhgozal@volcani.agri.gov.il</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gueta-Dahan</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Avsian-Kretchmer</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Weichert</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Feussner</LastName><ForeName>I</ForeName><Initials>I</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType 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