Changes in Dimethylsulfoniopropionate Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom ▿
Identifieur interne : 000354 ( Pmc/Curation ); précédent : 000353; suivant : 000355Changes in Dimethylsulfoniopropionate Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom ▿
Auteurs : Erinn C. Howard ; Shulei Sun ; Christopher R. Reisch ; Daniela A. Del Valle ; Helmut Bürgmann ; Ronald P. Kiene ; Mary Ann MoranSource :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2010.
Abstract
Over half of the bacterioplankton cells in ocean surface waters are capable of carrying out a demethylation of the phytoplankton metabolite dimethylsulfoniopropionate (DMSP) that routes the sulfur moiety away from the climatically active gas dimethylsulfide (DMS). In this study, we tracked changes in
Url:
DOI: 10.1128/AEM.01457-10
PubMed: 21097583
PubMed Central: 3020546
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<author><name sortKey="Howard, Erinn C" sort="Howard, Erinn C" uniqKey="Howard E" first="Erinn C." last="Howard">Erinn C. Howard</name>
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<author><name sortKey="Kiene, Ronald P" sort="Kiene, Ronald P" uniqKey="Kiene R" first="Ronald P." last="Kiene">Ronald P. Kiene</name>
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<author><name sortKey="Moran, Mary Ann" sort="Moran, Mary Ann" uniqKey="Moran M" first="Mary Ann" last="Moran">Mary Ann Moran</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Changes in Dimethylsulfoniopropionate Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom <xref ref-type="fn" rid="fn4">▿</xref>
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<author><name sortKey="Howard, Erinn C" sort="Howard, Erinn C" uniqKey="Howard E" first="Erinn C." last="Howard">Erinn C. Howard</name>
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<author><name sortKey="Burgmann, Helmut" sort="Burgmann, Helmut" uniqKey="Burgmann H" first="Helmut" last="Bürgmann">Helmut Bürgmann</name>
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<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
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<front><div type="abstract" xml:lang="en"><p>Over half of the bacterioplankton cells in ocean surface waters are capable of carrying out a demethylation of the phytoplankton metabolite dimethylsulfoniopropionate (DMSP) that routes the sulfur moiety away from the climatically active gas dimethylsulfide (DMS). In this study, we tracked changes in <italic>dmdA</italic>
, the gene responsible for DMSP demethylation, over the course of an induced phytoplankton bloom in Gulf of Mexico seawater microcosms. Analysis of >91,000 amplicon sequences indicated 578 different <italic>dmdA</italic>
sequence clusters at a conservative clustering criterion of ≥90% nucleotide sequence identity over the 6-day study. The representation of the major clades of <italic>dmdA</italic>
, several of which are linked to specific taxa through genomes of cultured marine bacterioplankton, remained fairly constant. However, the representation of clusters within these major clades shifted significantly in response to the bloom, including two <italic>Roseobacter</italic>
-like clusters and a SAR11-like cluster, and the best correlate with shifts of the dominant <italic>dmdA</italic>
clades was chlorophyll <italic>a</italic>
concentration. Concurrent 16S rRNA amplification and sequencing indicated the presence of <italic>Roseobacter</italic>
, SAR11, OM60, and marine <italic>Rhodospirillales</italic>
populations, all of which are known to harbor <italic>dmdA</italic>
genes, although the largest taxonomic change was an increase in <italic>Flavobacteriaceae</italic>
, a group not yet demonstrated to have DMSP-demethylating capabilities. Sequence heterogeneity in <italic>dmdA</italic>
and other functional gene populations is becoming increasingly evident with the advent of high-throughput sequencing technologies, and understanding the ecological implications of this heterogeneity is a major challenge for marine microbial ecology.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">aem</journal-id>
<journal-title-group><journal-title>Applied and Environmental Microbiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology (ASM)</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21097583</article-id>
<article-id pub-id-type="pmc">3020546</article-id>
<article-id pub-id-type="publisher-id">1457-10</article-id>
<article-id pub-id-type="doi">10.1128/AEM.01457-10</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Microbial Ecology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Changes in Dimethylsulfoniopropionate Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom <xref ref-type="fn" rid="fn4">▿</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Howard</surname>
<given-names>Erinn C.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="fn" rid="fn1">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sun</surname>
<given-names>Shulei</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="fn" rid="fn2">§</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Reisch</surname>
<given-names>Christopher R.</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>del Valle</surname>
<given-names>Daniela A.</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
<xref ref-type="fn" rid="fn3">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bürgmann</surname>
<given-names>Helmut</given-names>
</name>
<xref ref-type="aff" rid="aff1">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kiene</surname>
<given-names>Ronald P.</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Moran</surname>
<given-names>Mary Ann</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
</contrib-group>
<aff id="aff1">Department of Marine Sciences,<label>1</label>
Department of Microbiology, University of Georgia, Athens, Georgia 30602,<label>2</label>
Department of Marine Sciences, University of South Alabama, Mobile, Alabama 36688,<label>3</label>
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Surface Waters—Research and Management, CH-6047 Kastanienbaum, Switzerland<label>4</label>
</aff>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author. Mailing address: Department of Marine Sciences, University of Georgia, Athens, GA 30602. Phone: (706) 542-6481. Fax: (706) 542-5888. E-mail: <email>mmoran@uga.edu</email>
</corresp>
<fn id="fn1"><label>‡</label>
<p>Present address: U.S. Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375.</p>
</fn>
<fn id="fn2"><label>§</label>
<p>Present address: Center for Research in Biological Systems, University of California San Diego, 9500 Gilman Drive #0446, La Jolla, CA 92093.</p>
</fn>
<fn id="fn3"><label>†</label>
<p>Present address: School of Ocean and Earth Science and Technology (SOEST) and Center for Microbial Oceanography: Research and Education (C-MORE), University of Hawaii, Honolulu, HI 96822.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>1</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="epub"><day>19</day>
<month>11</month>
<year>2010</year>
</pub-date>
<volume>77</volume>
<issue>2</issue>
<fpage>524</fpage>
<lpage>531</lpage>
<history><date date-type="received"><day>18</day>
<month>6</month>
<year>2010</year>
</date>
<date date-type="accepted"><day>12</day>
<month>11</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2011, American Society for Microbiology</copyright-statement>
<copyright-year>2011</copyright-year>
</permissions>
<self-uri xlink:title="pdf" xlink:href="zam00211000524.pdf"></self-uri>
<abstract><p>Over half of the bacterioplankton cells in ocean surface waters are capable of carrying out a demethylation of the phytoplankton metabolite dimethylsulfoniopropionate (DMSP) that routes the sulfur moiety away from the climatically active gas dimethylsulfide (DMS). In this study, we tracked changes in <italic>dmdA</italic>
, the gene responsible for DMSP demethylation, over the course of an induced phytoplankton bloom in Gulf of Mexico seawater microcosms. Analysis of >91,000 amplicon sequences indicated 578 different <italic>dmdA</italic>
sequence clusters at a conservative clustering criterion of ≥90% nucleotide sequence identity over the 6-day study. The representation of the major clades of <italic>dmdA</italic>
, several of which are linked to specific taxa through genomes of cultured marine bacterioplankton, remained fairly constant. However, the representation of clusters within these major clades shifted significantly in response to the bloom, including two <italic>Roseobacter</italic>
-like clusters and a SAR11-like cluster, and the best correlate with shifts of the dominant <italic>dmdA</italic>
clades was chlorophyll <italic>a</italic>
concentration. Concurrent 16S rRNA amplification and sequencing indicated the presence of <italic>Roseobacter</italic>
, SAR11, OM60, and marine <italic>Rhodospirillales</italic>
populations, all of which are known to harbor <italic>dmdA</italic>
genes, although the largest taxonomic change was an increase in <italic>Flavobacteriaceae</italic>
, a group not yet demonstrated to have DMSP-demethylating capabilities. Sequence heterogeneity in <italic>dmdA</italic>
and other functional gene populations is becoming increasingly evident with the advent of high-throughput sequencing technologies, and understanding the ecological implications of this heterogeneity is a major challenge for marine microbial ecology.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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