Characterization of Cyanate Metabolism in Marine Synechococcus and Prochlorococcus spp. ▿
Identifieur interne : 000353 ( Pmc/Curation ); précédent : 000352; suivant : 000354Characterization of Cyanate Metabolism in Marine Synechococcus and Prochlorococcus spp. ▿
Auteurs : Nina A. Kamennaya ; Anton F. PostSource :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2010.
Abstract
Cyanobacteria of the genera
Url:
DOI: 10.1128/AEM.01272-10
PubMed: 21057026
PubMed Central: 3019706
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of Cyanate Metabolism in Marine <italic>Synechococcus</italic>
and <italic>Prochlorococcus</italic>
spp. <xref ref-type="fn" rid="fn1">▿</xref>
</title>
<author><name sortKey="Kamennaya, Nina A" sort="Kamennaya, Nina A" uniqKey="Kamennaya N" first="Nina A." last="Kamennaya">Nina A. Kamennaya</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Post, Anton F" sort="Post, Anton F" uniqKey="Post A" first="Anton F." last="Post">Anton F. Post</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
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<idno type="pmc">3019706</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of Cyanate Metabolism in Marine <italic>Synechococcus</italic>
and <italic>Prochlorococcus</italic>
spp. <xref ref-type="fn" rid="fn1">▿</xref>
</title>
<author><name sortKey="Kamennaya, Nina A" sort="Kamennaya, Nina A" uniqKey="Kamennaya N" first="Nina A." last="Kamennaya">Nina A. Kamennaya</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Post, Anton F" sort="Post, Anton F" uniqKey="Post A" first="Anton F." last="Post">Anton F. Post</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
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</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2010">2010</date>
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<front><div type="abstract" xml:lang="en"><p>Cyanobacteria of the genera <italic>Synechococcus</italic>
and <italic>Prochlorococcus</italic>
are the most abundant photosynthetic organisms on earth, occupying a key position at the base of marine food webs. The <italic>cynS</italic>
gene that encodes cyanase was identified among bacterial, fungal, and plant sequences in public databases, and the gene was particularly prevalent among cyanobacteria, including numerous <italic>Prochlorococcus</italic>
and <italic>Synechococcus</italic>
strains. Phylogenetic analysis of <italic>cynS</italic>
sequences retrieved from the Global Ocean Survey database identified >60% as belonging to unicellular marine cyanobacteria, suggesting an important role for cyanase in their nitrogen metabolism. We demonstrate here that marine cyanobacteria have a functionally active cyanase, the transcriptional regulation of which varies among strains and reflects the genomic context of <italic>cynS</italic>
. In <italic>Prochlorococcus</italic>
sp. strain MED4, <italic>cynS</italic>
was presumably transcribed as part of the <italic>cynABDS</italic>
operon, implying cyanase involvement in cyanate utilization. In <italic>Synechococcus</italic>
sp. strain WH8102, expression was not related to nitrogen stress responses and here cyanase presumably serves in the detoxification of cyanate resulting from intracellular urea and/or carbamoyl phosphate decomposition. Lastly, we report on a cyanase activity encoded by <italic>cynH</italic>
, a novel gene found in marine cyanobacteria only. The presence of dual cyanase genes in the genomes of seven marine <italic>Synechococcus</italic>
strains and their respective roles in nitrogen metabolism remain to be clarified.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">aem</journal-id>
<journal-title-group><journal-title>Applied and Environmental Microbiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology (ASM)</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21057026</article-id>
<article-id pub-id-type="pmc">3019706</article-id>
<article-id pub-id-type="publisher-id">1272-10</article-id>
<article-id pub-id-type="doi">10.1128/AEM.01272-10</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Genetics and Molecular Biology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Characterization of Cyanate Metabolism in Marine <italic>Synechococcus</italic>
and <italic>Prochlorococcus</italic>
spp. <xref ref-type="fn" rid="fn1">▿</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Kamennaya</surname>
<given-names>Nina A.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Post</surname>
<given-names>Anton F.</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
</contrib-group>
<aff id="aff1">Department of Plant and Environmental Sciences, Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel,<label>1</label>
Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543<label>2</label>
</aff>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author. Mailing address: Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL St., Woods Hole, MA 02543. Phone: (508) 289-7131. Fax: (508) 457-4727. E-mail: <email>apost@mbl.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>1</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="epub"><day>5</day>
<month>11</month>
<year>2010</year>
</pub-date>
<volume>77</volume>
<issue>1</issue>
<fpage>291</fpage>
<lpage>301</lpage>
<history><date date-type="received"><day>30</day>
<month>5</month>
<year>2010</year>
</date>
<date date-type="accepted"><day>27</day>
<month>10</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2011, American Society for Microbiology</copyright-statement>
<copyright-year>2011</copyright-year>
</permissions>
<self-uri xlink:title="pdf" xlink:href="zam00111000291.pdf"></self-uri>
<abstract><p>Cyanobacteria of the genera <italic>Synechococcus</italic>
and <italic>Prochlorococcus</italic>
are the most abundant photosynthetic organisms on earth, occupying a key position at the base of marine food webs. The <italic>cynS</italic>
gene that encodes cyanase was identified among bacterial, fungal, and plant sequences in public databases, and the gene was particularly prevalent among cyanobacteria, including numerous <italic>Prochlorococcus</italic>
and <italic>Synechococcus</italic>
strains. Phylogenetic analysis of <italic>cynS</italic>
sequences retrieved from the Global Ocean Survey database identified >60% as belonging to unicellular marine cyanobacteria, suggesting an important role for cyanase in their nitrogen metabolism. We demonstrate here that marine cyanobacteria have a functionally active cyanase, the transcriptional regulation of which varies among strains and reflects the genomic context of <italic>cynS</italic>
. In <italic>Prochlorococcus</italic>
sp. strain MED4, <italic>cynS</italic>
was presumably transcribed as part of the <italic>cynABDS</italic>
operon, implying cyanase involvement in cyanate utilization. In <italic>Synechococcus</italic>
sp. strain WH8102, expression was not related to nitrogen stress responses and here cyanase presumably serves in the detoxification of cyanate resulting from intracellular urea and/or carbamoyl phosphate decomposition. Lastly, we report on a cyanase activity encoded by <italic>cynH</italic>
, a novel gene found in marine cyanobacteria only. The presence of dual cyanase genes in the genomes of seven marine <italic>Synechococcus</italic>
strains and their respective roles in nitrogen metabolism remain to be clarified.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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