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Anaerobic biosynthesis of the lower ligand of vitamin B12

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Anaerobic biosynthesis of the lower ligand of vitamin B12

Auteurs : Amrita B. Hazra ; Andrew W. Han ; Angad P. Mehta ; Kenny C. Mok ; Vadim Osadchiy ; Tadhg P. Begley ; Michiko E. Taga

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2015.

RBID : PMC:4553811

Abstract

Significance

Vitamin B₁₂ is required by humans and a variety of other organisms for diverse metabolic processes, but is produced only by a subset of microorganisms. The anaerobic biosynthesis of the “lower ligand” of B₁₂, 5,6-dimethylbenzimidazole (DMB), is the only unknown component of the B₁₂ biosynthetic pathway. We report the identification of the bzaABCDE genes that are necessary and sufficient for the anaerobic biosynthesis of DMB. We have characterized the role of each of the bza genes and identified three intermediates in the pathway. This finding not only completes the B₁₂ biosynthesis pathway but also enables the sequence-based prediction of cobamides synthesized by anaerobic microorganisms.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4553811

DOI: 10.1073/pnas.1509132112
PubMed: 26246619
PubMed Central: 4553811

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PMC:4553811

Le document en format XML

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<author><name sortKey="Han, Andrew W" sort="Han, Andrew W" uniqKey="Han A" first="Andrew W." last="Han">Andrew W. Han</name>
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<author><name sortKey="Mehta, Angad P" sort="Mehta, Angad P" uniqKey="Mehta A" first="Angad P." last="Mehta">Angad P. Mehta</name>
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<author><name sortKey="Mok, Kenny C" sort="Mok, Kenny C" uniqKey="Mok K" first="Kenny C." last="Mok">Kenny C. Mok</name>
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<author><name sortKey="Osadchiy, Vadim" sort="Osadchiy, Vadim" uniqKey="Osadchiy V" first="Vadim" last="Osadchiy">Vadim Osadchiy</name>
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<author><name sortKey="Begley, Tadhg P" sort="Begley, Tadhg P" uniqKey="Begley T" first="Tadhg P." last="Begley">Tadhg P. Begley</name>
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<author><name sortKey="Taga, Michiko E" sort="Taga, Michiko E" uniqKey="Taga M" first="Michiko E." last="Taga">Michiko E. Taga</name>
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<author><name sortKey="Mehta, Angad P" sort="Mehta, Angad P" uniqKey="Mehta A" first="Angad P." last="Mehta">Angad P. Mehta</name>
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,<addr-line>CA</addr-line>
 94720;</nlm:aff>
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, College Station,<addr-line>TX</addr-line>
 77843</nlm:aff>
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<author><name sortKey="Taga, Michiko E" sort="Taga, Michiko E" uniqKey="Taga M" first="Michiko E." last="Taga">Michiko E. Taga</name>
<affiliation><nlm:aff id="aff1">Department of Plant and Microbial Biology,<institution>University of California, Berkeley</institution>
,<addr-line>CA</addr-line>
 94720;</nlm:aff>
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<front><div type="abstract" xml:lang="en"><title>Significance</title>
<p>Vitamin B<sub>12</sub>
 is required by humans and a variety of other organisms for diverse metabolic processes, but is produced only by a subset of microorganisms. The anaerobic biosynthesis of the “lower ligand” of B<sub>12</sub>
, 5,6-dimethylbenzimidazole (DMB), is the only unknown component of the B<sub>12</sub>
 biosynthetic pathway. We report the identification of the <italic>bzaABCDE</italic>
 genes that are necessary and sufficient for the anaerobic biosynthesis of DMB. We have characterized the role of each of the <italic>bza</italic>
 genes and identified three intermediates in the pathway. This finding not only completes the B<sub>12</sub>
 biosynthesis pathway but also enables the sequence-based prediction of cobamides synthesized by anaerobic microorganisms.</p>
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</front>
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Anaerobic biosynthesis of the lower ligand of vitamin B12

Anaerobic biosynthesis of the lower ligand of vitamin B12

Source :

Abstract

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