Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model
Identifieur interne : 000086 ( Pmc/Curation ); précédent : 000085; suivant : 000087Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model
Auteurs : Yuka Tsumanuma ; Takanori Iwata ; Atsuhiro Kinoshita ; Kaoru Washio ; Toshiyuki Yoshida ; Azusa Yamada ; Ryo Takagi ; Masayuki Yamato ; Teruo Okano ; Yuichi IzumiSource :
- BioResearch Open Access [ 2164-7844 ] ; 2016.
Abstract
Periodontitis is a chronic inflammatory disease that induces the destruction of tooth-supporting tissues, followed by tooth loss. Although several approaches have been applied to periodontal regeneration, complete periodontal regeneration has not been accomplished. Tissue engineering using a combination of cells and scaffolds is considered to be a viable alternative strategy. We have shown that autologous transplantation of periodontal ligament-derived multipotent mesenchymal stromal cell (PDL-MSC) sheets regenerates periodontal tissue in canine models. However, the indications for autologous cell transplantation in clinical situations are limited. Therefore, this study evaluated the safety and efficacy of allogeneic transplantation of PDL-MSC sheets using a canine horizontal periodontal defect model. Canine PDL-MSCs were labeled with enhanced green fluorescent protein (EGFP) and were cultured on temperature-responsive dishes. Three-layered cell sheets were transplanted around denuded root surfaces either autologously or allogeneically. A mixture of β-tricalcium phosphate and collagen gel was placed on the bone defects. Eight weeks after transplantation, dogs were euthanized and subjected to microcomputed tomography and histological analyses. RNA and DNA were extracted from the paraffin sections to verify the presence of EGFP at the transplantation site. Inflammatory markers from peripheral blood sera were quantified using an enzyme-linked immunosorbent assay. Periodontal regeneration was observed in both the autologous and the allogeneic transplantation groups. The allogeneic transplantation group showed particularly significant regeneration of newly formed cementum, which is critical for the periodontal regeneration. Serum levels of inflammatory markers from peripheral blood sera showed little difference between the autologous and allogeneic groups. EGFP amplicons were detectable in the paraffin sections of the allogeneic group. These results suggest that allogeneic PDL-MSC sheets promoted periodontal tissue regeneration without side effects. Therefore, allogeneic transplantation of PDL-MSC sheets has a potential to become an alternative strategy for periodontal regeneration.
Url:
DOI: 10.1089/biores.2015.0043
PubMed: 26862470
PubMed Central: 4744877
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model</title><author><name sortKey="Tsumanuma, Yuka" sort="Tsumanuma, Yuka" uniqKey="Tsumanuma Y" first="Yuka" last="Tsumanuma">Yuka Tsumanuma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Iwata, Takanori" sort="Iwata, Takanori" uniqKey="Iwata T" first="Takanori" last="Iwata">Takanori Iwata</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Kinoshita, Atsuhiro" sort="Kinoshita, Atsuhiro" uniqKey="Kinoshita A" first="Atsuhiro" last="Kinoshita">Atsuhiro Kinoshita</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Washio, Kaoru" sort="Washio, Kaoru" uniqKey="Washio K" first="Kaoru" last="Washio">Kaoru Washio</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yoshida, Toshiyuki" sort="Yoshida, Toshiyuki" uniqKey="Yoshida T" first="Toshiyuki" last="Yoshida">Toshiyuki Yoshida</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamada, Azusa" sort="Yamada, Azusa" uniqKey="Yamada A" first="Azusa" last="Yamada">Azusa Yamada</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Takagi, Ryo" sort="Takagi, Ryo" uniqKey="Takagi R" first="Ryo" last="Takagi">Ryo Takagi</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamato, Masayuki" sort="Yamato, Masayuki" uniqKey="Yamato M" first="Masayuki" last="Yamato">Masayuki Yamato</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Okano, Teruo" sort="Okano, Teruo" uniqKey="Okano T" first="Teruo" last="Okano">Teruo Okano</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Izumi, Yuichi" sort="Izumi, Yuichi" uniqKey="Izumi Y" first="Yuichi" last="Izumi">Yuichi Izumi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26862470</idno><idno type="pmc">4744877</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744877</idno><idno type="RBID">PMC:4744877</idno><idno type="doi">10.1089/biores.2015.0043</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000086</idno><idno type="wicri:Area/Pmc/Curation">000086</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model</title><author><name sortKey="Tsumanuma, Yuka" sort="Tsumanuma, Yuka" uniqKey="Tsumanuma Y" first="Yuka" last="Tsumanuma">Yuka Tsumanuma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Iwata, Takanori" sort="Iwata, Takanori" uniqKey="Iwata T" first="Takanori" last="Iwata">Takanori Iwata</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Kinoshita, Atsuhiro" sort="Kinoshita, Atsuhiro" uniqKey="Kinoshita A" first="Atsuhiro" last="Kinoshita">Atsuhiro Kinoshita</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Washio, Kaoru" sort="Washio, Kaoru" uniqKey="Washio K" first="Kaoru" last="Washio">Kaoru Washio</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yoshida, Toshiyuki" sort="Yoshida, Toshiyuki" uniqKey="Yoshida T" first="Toshiyuki" last="Yoshida">Toshiyuki Yoshida</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamada, Azusa" sort="Yamada, Azusa" uniqKey="Yamada A" first="Azusa" last="Yamada">Azusa Yamada</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Takagi, Ryo" sort="Takagi, Ryo" uniqKey="Takagi R" first="Ryo" last="Takagi">Ryo Takagi</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamato, Masayuki" sort="Yamato, Masayuki" uniqKey="Yamato M" first="Masayuki" last="Yamato">Masayuki Yamato</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Okano, Teruo" sort="Okano, Teruo" uniqKey="Okano T" first="Teruo" last="Okano">Teruo Okano</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Izumi, Yuichi" sort="Izumi, Yuichi" uniqKey="Izumi Y" first="Yuichi" last="Izumi">Yuichi Izumi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">BioResearch Open Access</title><idno type="ISSN">2164-7844</idno><idno type="eISSN">2164-7860</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Periodontitis is a chronic inflammatory disease that induces the destruction of tooth-supporting tissues, followed by tooth loss. Although several approaches have been applied to periodontal regeneration, complete periodontal regeneration has not been accomplished. Tissue engineering using a combination of cells and scaffolds is considered to be a viable alternative strategy. We have shown that autologous transplantation of periodontal ligament-derived multipotent mesenchymal stromal cell (PDL-MSC) sheets regenerates periodontal tissue in canine models. However, the indications for autologous cell transplantation in clinical situations are limited. Therefore, this study evaluated the safety and efficacy of allogeneic transplantation of PDL-MSC sheets using a canine horizontal periodontal defect model. Canine PDL-MSCs were labeled with enhanced green fluorescent protein (EGFP) and were cultured on temperature-responsive dishes. Three-layered cell sheets were transplanted around denuded root surfaces either autologously or allogeneically. A mixture of β-tricalcium phosphate and collagen gel was placed on the bone defects. Eight weeks after transplantation, dogs were euthanized and subjected to microcomputed tomography and histological analyses. RNA and DNA were extracted from the paraffin sections to verify the presence of EGFP at the transplantation site. Inflammatory markers from peripheral blood sera were quantified using an enzyme-linked immunosorbent assay. Periodontal regeneration was observed in both the autologous and the allogeneic transplantation groups. The allogeneic transplantation group showed particularly significant regeneration of newly formed cementum, which is critical for the periodontal regeneration. Serum levels of inflammatory markers from peripheral blood sera showed little difference between the autologous and allogeneic groups. EGFP amplicons were detectable in the paraffin sections of the allogeneic group. These results suggest that allogeneic PDL-MSC sheets promoted periodontal tissue regeneration without side effects. Therefore, allogeneic transplantation of PDL-MSC sheets has a potential to become an alternative strategy for periodontal regeneration.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hajishengallis, G" uniqKey="Hajishengallis G">G Hajishengallis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lalla, E" uniqKey="Lalla E">E Lalla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lockhart, Pb" uniqKey="Lockhart P">PB Lockhart</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koop, R" uniqKey="Koop R">R Koop</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramseier, Ca" uniqKey="Ramseier C">CA Ramseier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Fm" uniqKey="Chen F">FM Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biores Open Access</journal-id><journal-id journal-id-type="iso-abbrev">Biores Open Access</journal-id><journal-id journal-id-type="publisher-id">biores</journal-id><journal-title-group><journal-title>BioResearch Open Access</journal-title></journal-title-group><issn pub-type="ppub">2164-7844</issn><issn pub-type="epub">2164-7860</issn><publisher><publisher-name>Mary Ann Liebert, Inc.</publisher-name><publisher-loc>140 Huguenot Street, 3rd FloorNew Rochelle, NY 10801USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26862470</article-id><article-id pub-id-type="pmc">4744877</article-id><article-id pub-id-type="publisher-id">10.1089/biores.2015.0043</article-id><article-id pub-id-type="doi">10.1089/biores.2015.0043</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Research Article</subject></subj-group></article-categories><title-group><article-title>Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tsumanuma</surname><given-names>Yuka</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Iwata</surname><given-names>Takanori</given-names></name><xref ref-type="aff" rid="aff2"><sup>2,</sup></xref><xref ref-type="corresp" rid="corr1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kinoshita</surname><given-names>Atsuhiro</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Washio</surname><given-names>Kaoru</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yoshida</surname><given-names>Toshiyuki</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yamada</surname><given-names>Azusa</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Takagi</surname><given-names>Ryo</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yamato</surname><given-names>Masayuki</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Okano</surname><given-names>Teruo</given-names></name><xref ref-type="aff" rid="aff2"><sup>2,</sup></xref><xref ref-type="corresp" rid="corr1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Izumi</surname><given-names>Yuichi</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><aff id="aff1"><label><sup>1</sup></label>Department of Periodontology, Graduate School of Medical and Dental Sciences,<institution>Tokyo Medical and Dental University</institution>, Tokyo,<country>Japan</country>.</aff><aff id="aff2"><label><sup>2</sup></label>Institute of Advanced Biomedical Engineering and Science,<institution>Tokyo Women's Medical University</institution>, Tokyo,<country>Japan</country>.</aff><aff id="aff3"><label><sup>3</sup></label>Department of Educational Media Development, Graduate School of Medical and Dental Sciences,<institution>Tokyo Medical and Dental University</institution>, Tokyo,<country>Japan</country>.</aff><aff id="aff4"><label><sup>4</sup></label>Department of Behavioral Dentistry, Graduate School of Medical and Dental Sciences,<institution>Tokyo Medical and Dental University</institution>, Tokyo,<country>Japan</country>.</aff></contrib-group><author-notes><corresp id="corr1"><label><sup>*</sup></label>Address correspondence to: Takanori Iwata, DDS, PhD, Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan, E-mail: <uri xlink:type="simple" xlink:href="http://iwata.takanori@twmu.ac.jp">iwata.takanori@twmu.ac.jp</uri><italic>or</italic> Teruo Okano, PhD, Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan, E-mail: <email xlink:href="mailto:tokano@twmu.ac.jp">tokano@twmu.ac.jp</email></corresp></author-notes><pub-date pub-type="epub"><day>01</day><month>1</month><year>2016</year><pmc-comment>string-date: January 2016</pmc-comment>
</pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>1</month><year>2016</year><pmc-comment>string-date: January 2016</pmc-comment>
</pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>5</volume><issue>1</issue><fpage>22</fpage><lpage>36</lpage><permissions><copyright-statement>© Yuka Tsumanuma <italic>et al.</italic> 2016; Published by Mary Ann Liebert, Inc.</copyright-statement><copyright-year>2016</copyright-year><license license-type="open-access"><license-p>This Open Access article is distributed under the terms of the Creative Commons License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="biores.2015.0043.pdf"></self-uri><abstract><title>Abstract</title><p>Periodontitis is a chronic inflammatory disease that induces the destruction of tooth-supporting tissues, followed by tooth loss. Although several approaches have been applied to periodontal regeneration, complete periodontal regeneration has not been accomplished. Tissue engineering using a combination of cells and scaffolds is considered to be a viable alternative strategy. We have shown that autologous transplantation of periodontal ligament-derived multipotent mesenchymal stromal cell (PDL-MSC) sheets regenerates periodontal tissue in canine models. However, the indications for autologous cell transplantation in clinical situations are limited. Therefore, this study evaluated the safety and efficacy of allogeneic transplantation of PDL-MSC sheets using a canine horizontal periodontal defect model. Canine PDL-MSCs were labeled with enhanced green fluorescent protein (EGFP) and were cultured on temperature-responsive dishes. Three-layered cell sheets were transplanted around denuded root surfaces either autologously or allogeneically. A mixture of β-tricalcium phosphate and collagen gel was placed on the bone defects. Eight weeks after transplantation, dogs were euthanized and subjected to microcomputed tomography and histological analyses. RNA and DNA were extracted from the paraffin sections to verify the presence of EGFP at the transplantation site. Inflammatory markers from peripheral blood sera were quantified using an enzyme-linked immunosorbent assay. Periodontal regeneration was observed in both the autologous and the allogeneic transplantation groups. The allogeneic transplantation group showed particularly significant regeneration of newly formed cementum, which is critical for the periodontal regeneration. Serum levels of inflammatory markers from peripheral blood sera showed little difference between the autologous and allogeneic groups. EGFP amplicons were detectable in the paraffin sections of the allogeneic group. These results suggest that allogeneic PDL-MSC sheets promoted periodontal tissue regeneration without side effects. Therefore, allogeneic transplantation of PDL-MSC sheets has a potential to become an alternative strategy for periodontal regeneration.</p></abstract><kwd-group kwd-group-type="author"><title><bold>Key words:</bold> </title><kwd>regeneration</kwd><kwd>stem cells</kwd><kwd>tissue engineering</kwd></kwd-group><counts><fig-count count="7"></fig-count><table-count count="1"></table-count><ref-count count="54"></ref-count><page-count count="15"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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