Radioactive Holmium Acetylacetonate Microspheres for Interstitial Microbrachytherapy: An In Vitro and In Vivo Stability Study
Identifieur interne : 000182 ( Pmc/Corpus ); précédent : 000181; suivant : 000183Radioactive Holmium Acetylacetonate Microspheres for Interstitial Microbrachytherapy: An In Vitro and In Vivo Stability Study
Auteurs : Wouter Bult ; Hendrik De Leeuw ; Olav M. Steinebach ; Martijn J. Van Der Bom ; Hubert Th. Wolterbeek ; Ron M. A. Heeren ; Chris J. G. Bakker ; Alfred D. Van Het Schip ; Wim E. Hennink ; J. Frank W. NijsenSource :
- Pharmaceutical Research [ 0724-8741 ] ; 2011.
Abstract
The clinical application of holmium acetylacetonate microspheres (HoAcAcMS) for the intratumoral radionuclide treatment of solid malignancies requires a thorough understanding of their stability. Therefore, an
HoAcAcMS, before and after neutron irradiation, were incubated in a phosphate buffer at 37°C for 6 months. The
After 4 days in buffer the acetylacetonate ligands were replaced by phosphate, without altering the particle size and surface morphology. HoAcAcMS before and after neutron irradiation were administered intratumorally in VX2 tumor-bearing rabbits. No holmium was detected in the faeces, urine, femur and blood. Histological examination of the tumor revealed clusters of intact microspheres amidst necrotic tissue after 30 days.
HoAcAcMS are stable both
Url:
DOI: 10.1007/s11095-011-0610-7
PubMed: 22068276
PubMed Central: 3281200
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Radioactive Holmium Acetylacetonate Microspheres for Interstitial Microbrachytherapy: An <italic>In Vitro</italic> and <italic>In Vivo</italic> Stability Study</title><author><name sortKey="Bult, Wouter" sort="Bult, Wouter" uniqKey="Bult W" first="Wouter" last="Bult">Wouter Bult</name><affiliation><nlm:aff id="Aff1">Department of Radiology and Nuclear Medicine, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="De Leeuw, Hendrik" sort="De Leeuw, Hendrik" uniqKey="De Leeuw H" first="Hendrik" last="De Leeuw">Hendrik De Leeuw</name><affiliation><nlm:aff id="Aff2">Image Sciences Institute, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Steinebach, Olav M" sort="Steinebach, Olav M" uniqKey="Steinebach O" first="Olav M." last="Steinebach">Olav M. Steinebach</name><affiliation><nlm:aff id="Aff3">Department of Radiation, Radionuclides and Reactors Section Radiation & Isotopes for Health, Delft University of Technology, Delft, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Der Bom, Martijn J" sort="Van Der Bom, Martijn J" uniqKey="Van Der Bom M" first="Martijn J." last="Van Der Bom">Martijn J. Van Der Bom</name><affiliation><nlm:aff id="Aff2">Image Sciences Institute, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Wolterbeek, Hubert Th" sort="Wolterbeek, Hubert Th" uniqKey="Wolterbeek H" first="Hubert Th." last="Wolterbeek">Hubert Th. Wolterbeek</name><affiliation><nlm:aff id="Aff3">Department of Radiation, Radionuclides and Reactors Section Radiation & Isotopes for Health, Delft University of Technology, Delft, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Heeren, Ron M A" sort="Heeren, Ron M A" uniqKey="Heeren R" first="Ron M. A." last="Heeren">Ron M. A. Heeren</name><affiliation><nlm:aff id="Aff4">FOM institute for Atomic and Molecular Physics, Amsterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Bakker, Chris J G" sort="Bakker, Chris J G" uniqKey="Bakker C" first="Chris J. G." last="Bakker">Chris J. G. Bakker</name><affiliation><nlm:aff id="Aff2">Image Sciences Institute, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Het Schip, Alfred D" sort="Van Het Schip, Alfred D" uniqKey="Van Het Schip A" first="Alfred D." last="Van Het Schip">Alfred D. Van Het Schip</name><affiliation><nlm:aff id="Aff1">Department of Radiology and Nuclear Medicine, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Hennink, Wim E" sort="Hennink, Wim E" uniqKey="Hennink W" first="Wim E." last="Hennink">Wim E. Hennink</name><affiliation><nlm:aff id="Aff5">Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences Utrecht University, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Nijsen, J Frank W" sort="Nijsen, J Frank W" uniqKey="Nijsen J" first="J. Frank W." last="Nijsen">J. Frank W. Nijsen</name><affiliation><nlm:aff id="Aff1">Department of Radiology and Nuclear Medicine, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22068276</idno><idno type="pmc">3281200</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281200</idno><idno type="RBID">PMC:3281200</idno><idno type="doi">10.1007/s11095-011-0610-7</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000182</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Radioactive Holmium Acetylacetonate Microspheres for Interstitial Microbrachytherapy: An <italic>In Vitro</italic> and <italic>In Vivo</italic> Stability Study</title><author><name sortKey="Bult, Wouter" sort="Bult, Wouter" uniqKey="Bult W" first="Wouter" last="Bult">Wouter Bult</name><affiliation><nlm:aff id="Aff1">Department of Radiology and Nuclear Medicine, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="De Leeuw, Hendrik" sort="De Leeuw, Hendrik" uniqKey="De Leeuw H" first="Hendrik" last="De Leeuw">Hendrik De Leeuw</name><affiliation><nlm:aff id="Aff2">Image Sciences Institute, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Steinebach, Olav M" sort="Steinebach, Olav M" uniqKey="Steinebach O" first="Olav M." last="Steinebach">Olav M. Steinebach</name><affiliation><nlm:aff id="Aff3">Department of Radiation, Radionuclides and Reactors Section Radiation & Isotopes for Health, Delft University of Technology, Delft, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Der Bom, Martijn J" sort="Van Der Bom, Martijn J" uniqKey="Van Der Bom M" first="Martijn J." last="Van Der Bom">Martijn J. Van Der Bom</name><affiliation><nlm:aff id="Aff2">Image Sciences Institute, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Wolterbeek, Hubert Th" sort="Wolterbeek, Hubert Th" uniqKey="Wolterbeek H" first="Hubert Th." last="Wolterbeek">Hubert Th. Wolterbeek</name><affiliation><nlm:aff id="Aff3">Department of Radiation, Radionuclides and Reactors Section Radiation & Isotopes for Health, Delft University of Technology, Delft, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Heeren, Ron M A" sort="Heeren, Ron M A" uniqKey="Heeren R" first="Ron M. A." last="Heeren">Ron M. A. Heeren</name><affiliation><nlm:aff id="Aff4">FOM institute for Atomic and Molecular Physics, Amsterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Bakker, Chris J G" sort="Bakker, Chris J G" uniqKey="Bakker C" first="Chris J. G." last="Bakker">Chris J. G. Bakker</name><affiliation><nlm:aff id="Aff2">Image Sciences Institute, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Het Schip, Alfred D" sort="Van Het Schip, Alfred D" uniqKey="Van Het Schip A" first="Alfred D." last="Van Het Schip">Alfred D. Van Het Schip</name><affiliation><nlm:aff id="Aff1">Department of Radiology and Nuclear Medicine, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Hennink, Wim E" sort="Hennink, Wim E" uniqKey="Hennink W" first="Wim E." last="Hennink">Wim E. Hennink</name><affiliation><nlm:aff id="Aff5">Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences Utrecht University, Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Nijsen, J Frank W" sort="Nijsen, J Frank W" uniqKey="Nijsen J" first="J. Frank W." last="Nijsen">J. Frank W. Nijsen</name><affiliation><nlm:aff id="Aff1">Department of Radiology and Nuclear Medicine, UMC Utrecht, Utrecht, The Netherlands</nlm:aff></affiliation></author></analytic><series><title level="j">Pharmaceutical Research</title><idno type="ISSN">0724-8741</idno><idno type="eISSN">1573-904X</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><sec><title>Purpose</title><p>The clinical application of holmium acetylacetonate microspheres (HoAcAcMS) for the intratumoral radionuclide treatment of solid malignancies requires a thorough understanding of their stability. Therefore, an <italic>in vitro</italic> and an <italic>in vivo</italic> stability study with HoAcAcMS was conducted.</p></sec><sec><title>Methods</title><p>HoAcAcMS, before and after neutron irradiation, were incubated in a phosphate buffer at 37°C for 6 months. The <italic>in vitro</italic> release of holmium in this buffer after 6 months was 0.5%. Elemental analysis, scanning electron microscopy, infrared spectroscopy and time of flight secondary ion mass spectrometry were performed on the HoAcAcMS.</p></sec><sec><title>Results</title><p>After 4 days in buffer the acetylacetonate ligands were replaced by phosphate, without altering the particle size and surface morphology. HoAcAcMS before and after neutron irradiation were administered intratumorally in VX2 tumor-bearing rabbits. No holmium was detected in the faeces, urine, femur and blood. Histological examination of the tumor revealed clusters of intact microspheres amidst necrotic tissue after 30 days.</p></sec><sec><title>Conclusion</title><p>HoAcAcMS are stable both <italic>in vitro</italic> and <italic>in vivo</italic> and are suitable for intratumoral radionuclide treatment.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Jemal, A" uniqKey="Jemal A">A Jemal</name></author><author><name sortKey="Siegel, R" uniqKey="Siegel R">R Siegel</name></author><author><name sortKey="Ward, E" uniqKey="Ward E">E Ward</name></author><author><name sortKey="Hao, Y" uniqKey="Hao Y">Y Hao</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J Xu</name></author><author><name sortKey="Thun, Mj" uniqKey="Thun M">MJ Thun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parkin, Dm" uniqKey="Parkin D">DM Parkin</name></author><author><name sortKey="Bray, F" uniqKey="Bray F">F 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Pharm Res</journal-id><journal-title-group><journal-title>Pharmaceutical Research</journal-title></journal-title-group><issn pub-type="ppub">0724-8741</issn><issn pub-type="epub">1573-904X</issn><publisher><publisher-name>Springer US</publisher-name><publisher-loc>Boston</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22068276</article-id><article-id pub-id-type="pmc">3281200</article-id><article-id pub-id-type="publisher-id">610</article-id><article-id pub-id-type="doi">10.1007/s11095-011-0610-7</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject></subj-group></article-categories><title-group><article-title>Radioactive Holmium Acetylacetonate Microspheres for Interstitial Microbrachytherapy: An <italic>In Vitro</italic> and <italic>In Vivo</italic> Stability Study</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Bult</surname><given-names>Wouter</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>de Leeuw</surname><given-names>Hendrik</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Steinebach</surname><given-names>Olav M.</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>van der Bom</surname><given-names>Martijn J.</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Wolterbeek</surname><given-names>Hubert Th.</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Heeren</surname><given-names>Ron M. A.</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Bakker</surname><given-names>Chris J. G.</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>van het Schip</surname><given-names>Alfred D.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hennink</surname><given-names>Wim E.</given-names></name><xref ref-type="aff" rid="Aff5">5</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Nijsen</surname><given-names>J. Frank W.</given-names></name><address><phone>+31-88-7556295</phone><fax>+31-88-7542531</fax><email>f.nijsen@umcutrecht.nl</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label>Department of Radiology and Nuclear Medicine, UMC Utrecht, Utrecht, The Netherlands</aff><aff id="Aff2"><label>2</label>Image Sciences Institute, UMC Utrecht, Utrecht, The Netherlands</aff><aff id="Aff3"><label>3</label>Department of Radiation, Radionuclides and Reactors Section Radiation & Isotopes for Health, Delft University of Technology, Delft, The Netherlands</aff><aff id="Aff4"><label>4</label>FOM institute for Atomic and Molecular Physics, Amsterdam, The Netherlands</aff><aff id="Aff5"><label>5</label>Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences Utrecht University, Utrecht, The Netherlands</aff></contrib-group><pub-date pub-type="epub"><day>9</day><month>11</month><year>2011</year></pub-date><pub-date pub-type="pmc-release"><day>9</day><month>11</month><year>2011</year></pub-date><pub-date pub-type="ppub"><month>3</month><year>2012</year></pub-date><volume>29</volume><issue>3</issue><fpage>827</fpage><lpage>836</lpage><history><date date-type="received"><day>23</day><month>5</month><year>2011</year></date><date date-type="accepted"><day>17</day><month>10</month><year>2011</year></date></history><permissions><copyright-statement>© The Author(s) 2011</copyright-statement></permissions><abstract id="Abs1"><title>ABSTRACT</title><sec><title>Purpose</title><p>The clinical application of holmium acetylacetonate microspheres (HoAcAcMS) for the intratumoral radionuclide treatment of solid malignancies requires a thorough understanding of their stability. Therefore, an <italic>in vitro</italic> and an <italic>in vivo</italic> stability study with HoAcAcMS was conducted.</p></sec><sec><title>Methods</title><p>HoAcAcMS, before and after neutron irradiation, were incubated in a phosphate buffer at 37°C for 6 months. The <italic>in vitro</italic> release of holmium in this buffer after 6 months was 0.5%. Elemental analysis, scanning electron microscopy, infrared spectroscopy and time of flight secondary ion mass spectrometry were performed on the HoAcAcMS.</p></sec><sec><title>Results</title><p>After 4 days in buffer the acetylacetonate ligands were replaced by phosphate, without altering the particle size and surface morphology. HoAcAcMS before and after neutron irradiation were administered intratumorally in VX2 tumor-bearing rabbits. No holmium was detected in the faeces, urine, femur and blood. Histological examination of the tumor revealed clusters of intact microspheres amidst necrotic tissue after 30 days.</p></sec><sec><title>Conclusion</title><p>HoAcAcMS are stable both <italic>in vitro</italic> and <italic>in vivo</italic> and are suitable for intratumoral radionuclide treatment.</p></sec></abstract><kwd-group><title>KEY WORDS</title><kwd>brachytherapy</kwd><kwd>holmium</kwd><kwd><italic>in vivo</italic></kwd><kwd>microspheres</kwd><kwd>VX2 carcinoma</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media, LLC 2012</meta-value></custom-meta></custom-meta-group></article-meta></front><body><sec id="Sec1"><title>INTRODUCTION</title><p>Worldwide, cancer is the second cause of death, after cardiovascular diseases (<xref ref-type="bibr" rid="CR1">1</xref>), and it is estimated that each year over 20 million new cases are presented (<xref ref-type="bibr" rid="CR2">2</xref>). The majority (over 90%) of these new cases are solid tumors, and each year over 12 million people die from solid tumors (<xref ref-type="bibr" rid="CR1">1</xref>,<xref ref-type="bibr" rid="CR2">2</xref>). Traditionally, surgery has been the preferred treatment of solid malignancies, since this treatment can be considered curative (<xref ref-type="bibr" rid="CR3">3</xref>), but, unfortunately, not all tumors are eligible for curative resection. In the 1990s minimally invasive treatment options were proposed as treatment of inoperable solid malignancies, to offer a potentially curative treatment and to reduce the morbidity associated with surgery. These minimally invasive treatments, which are increasingly applied nowadays, include radiofrequency ablation (<xref ref-type="bibr" rid="CR4">4</xref>), high intensity focused ultrasound (<xref ref-type="bibr" rid="CR5">5</xref>) and local administration of radionuclides (<xref ref-type="bibr" rid="CR6">6</xref>,<xref ref-type="bibr" rid="CR7">7</xref>). With respect to the latter category, the most widely used technique is radioembolization of liver malignancies using radioactive microspheres (<xref ref-type="bibr" rid="CR8">8</xref>,<xref ref-type="bibr" rid="CR9">9</xref>). This technique involves the administration of yttrium-90 (<sup>90</sup>Y) microspheres using a catheter placed in the hepatic artery. Another approach is the direct intratumoral injection of radiolabeled particles or substances. Intratumorally administered <sup>90</sup>Y glass microspheres have been successfully tested in humans (<xref ref-type="bibr" rid="CR6">6</xref>). The results were promising: 90.6% of the tumors treated showed a size reduction, and apparent complete tumor necrosis was observed in 8 patients. Although these results were very promising, the extrahepatic delivery of microspheres, especially to the lungs, and the lack of accurate dose estimations were posed as problems to be overcome. Also, intratumoral administration of holmium-166 (<sup>166</sup>Ho) chelated to chitosan, an <italic>in situ</italic> gel forming device, has been tested in hepatocellular carcinoma patients with small (≤3 cm) solitary lesions (<xref ref-type="bibr" rid="CR10">10</xref>). Complete tumor necrosis was observed in 31 of 40 patients, yet the release of holmium from the injection site led to haematologic side effects in 11 of 40 patients. In itself, <sup>166</sup>Ho is an ideal isotope since, in contrast to <sup>90</sup>Y, it combines the emission of therapeutic beta particles with gamma rays. The gamma rays can be accurately visualized using gamma scintigraphy, which can be used for accurate dosimetric calculations. Moreover, holmium is a paramagnetic and electron dense element, allowing for magnetic resonance imaging and x-ray computed tomography (CT), respectively (<xref ref-type="bibr" rid="CR11">11</xref>). Chitosan as a delivery device for <sup>166</sup>Ho however is not ideal due to leakage of <sup>166</sup>Ho from the complex, causing haematologic side effects. To further optimize the intratumoral application of <sup>166</sup>Ho, holmium microspheres with an ultra high holmium load were proposed as a radioablation device for solid malignancies (<xref ref-type="bibr" rid="CR12">12</xref>). These microspheres were suitable to deliver sufficient radiation after intratumoral administration, and their efficacy was tested in a renal cell carcinoma tumor-bearing mouse model (<xref ref-type="bibr" rid="CR13">13</xref>). The microsphere treated tumors did not show a significant growth (141 ± 99 mm<sup>3</sup> at time of treatment <italic>versus</italic> 104 ± 95 mm<sup>3</sup> after 2 weeks), whereas the tumor size in the saline treated control group increased dramatically (from 122 ± 33 mm<sup>3</sup> to 4,150 ± 300 mm<sup>3</sup> 2 weeks post-injection). In addition to the laboratory animal study, a pilot experiment was performed to assess the feasibility and tolerability of intratumoral administration of <sup>166</sup>HoAcAcMS in feline liver cancer patients (<xref ref-type="bibr" rid="CR14">14</xref>). The treatment was well tolerated, and the clinical condition of the cats improved. It was concluded that intratumoral administration of <sup>166</sup>HoAcAcMS is efficacious, and not associated with adverse effects.</p><p>The purpose of the present study was to investigate the long-term <italic>in vitro</italic> stability of these radioactive holmium acetylacetonate microspheres (<sup>166</sup>HoAcAcMS). The holmium release from the HoAcAcMS was measured and the surface morphology and the chemical composition of the HoAcAcMS were investigated upon incubation of both non-neutron activated and neutron activated radioactive HoAcAcMS in phosphate buffer at 37°C for up to 26 weeks. The <italic>in vivo</italic> stability was assessed in VX-2 tumor-bearing rabbits, after intratumoral administration of both non-activated HoAcAcMS and neutron-activated HoAcAcMS. The rabbits were followed for 1 month, during which blood, urine and faeces were collected to determine the excretion of holmium. Histology was performed on tumor tissue, and the holmium content in the femur was determined.</p></sec><sec id="Sec2" sec-type="materials|methods"><title>MATERIALS AND METHODS</title><sec id="Sec3"><title>Materials</title><p>All chemicals were commercially available and used as received. Acetylacetone (2,4-pentanedione (AcAc; >99%)), ammonium hydroxide (NH<sub>4</sub>OH; 29.3% in water), chloroform (CHCl<sub>3</sub>; HPLC-grade), holmium phosphate (anhydrous (HoPO<sub>4</sub>; >99.99 + %), poly(vinyl alcohol) (PVA; average MW 30,000–70,000, 88% hydrolyzed) and poloxamer 188 (Pluronic<sup>®</sup> F68, average MW 8350) were purchased from Sigma Aldrich (Steinheim, Germany). Di-sodium hydrogen phosphate dihydrate (Na<sub>2</sub>HPO<sub>4</sub>.2H<sub>2</sub>O; 99%), ethanol (absolute), holmium CertiPUR ICP Standard (1.00 g L<sup>−1</sup>), sodium azide (NaN<sub>3</sub>; 99%) and sodium dihydrogen phosphate dihydrate (NaH<sub>2</sub>PO<sub arrange="stack">4</sub><sup arrange="stack">.</sup>2H<sub>2</sub>O; 99%) were purchased from Merck (Darmstadt, Germany). Holmium chloride hexahydrate (HoCl<sub>3</sub>.6H<sub>2</sub>O) was obtained from Metall rare earth (Shenzhen, China). Dexmedetomedine (Dexdomitor) and carprofen (Rimadyl) were obtained from Pfizer Animal Health B.V. (Capelle a/d IJssel, The Netherlands) and Isoflurane (IsoFlo) was obtained from Abbott Animal Health (Hoofddorp, The Netherlands).</p></sec><sec id="Sec4"><title>Preparation and Neutron Irradiation of HoAcAcMS</title><p>HoAcAcMS with a size of 15 μm were prepared by a solvent evaporation process previously described (<xref ref-type="bibr" rid="CR12">12</xref>). The HoAcAcMS (300 or 600 mg) were weighed in high density polyethylene vials (Type A, Posthumus plastics, Beverwijk, the Netherlands). Neutron irradiations were performed in the pneumatic rabbit system operational at the research reactor facility of the Reactor Institute Delft (Delft University of Technology, The Netherlands) (<xref ref-type="bibr" rid="CR15">15</xref>). Samples were irradiated for either 3 h or 6 h with a thermal neutron flux of 5 × 10<sup>12</sup> n cm<sup>−2</sup> s<sup>−1</sup>, and the radioactive <sup>166</sup>Ho was left to decay for 1 month in closed vials.</p></sec><sec id="Sec5"><title><italic>In Vitro</italic> Degradation of the Microspheres</title><p>The release of Ho<sup>3+</sup>-ions from HoAcAcMS was determined as described by Zielhuis <italic>et al</italic>. (<xref ref-type="bibr" rid="CR16">16</xref>). Neutron irradiated microsphere samples (50 mg) were suspended in 100 μl of 2% Pluronic<sup>®</sup> F68 aqueous solution and incubated in test tubes containing 5 mL isotonic phosphate buffer (116 mM, 39 mmol of NaH<sub>2</sub>PO<sub>4</sub>·2H<sub>2</sub>O and 77 mmol of Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O in 1 L, pH 7.4) with 0.05% NaN<sub>3</sub> to prevent bacterial growth. All samples were prepared in duplicate. The tubes were placed in a water bath at 37°C and continuously shaken and at predetermined time points (1 day, 4 days, 1, 2, 4, 8, 12 and 24 weeks) the test tubes were centrifuged, and the supernatant was collected. The microspheres were washed three times with water for injection and dried at 50°C. The microspheres and the supernatant were used for further analysis.</p></sec><sec id="Sec6"><title>Particle Surface Morphology</title><p>Surface morphology of the HoAcAcMS after incubation was studied using a Phenom (Phenom World BV, Eindhoven, The Netherlands) scanning electron microscope. The samples were placed on aluminium stubs and sputter coated with a 6 nm Pt layer, and images were acquired at a voltage of 5 kV.</p></sec><sec id="Sec7"><title>Elemental Analysis</title><p>The holmium, carbon, oxygen, hydrogen and phosphorous content was determined before and after incubation of HoAcAcMS in phosphate buffer by H. Kolbe (Mulheim an der Ruhr, Germany).</p></sec><sec id="Sec8"><title>Infrared Spectroscopy</title><p>Fourier Transform Infrared spectroscopy was used to ascertain the presence of acetylacetonate in the microspheres. Infrared spectra of dried microspheres were acquired on a BIO-RAD FTS6000 FT-IR (BIO-RAD, Cambridge, MA, USA). The spectra were recorded from 400 to 4,000 cm<sup>−1</sup> using KBr pellets, by accumulating 256 scans per spectrum.</p></sec><sec id="Sec9"><title>Secondary Ion Mass Spectrometry</title><p>Time of flight secondary ion mass spectrometry (TOF-SIMS) analysis was performed to obtain mass spectral information about the molecular composition and distribution on the surface of the microspheres before and after incubation in buffer (<xref ref-type="bibr" rid="CR17">17</xref>). Samples were fixed on a conductive carbon sticker on a stainless steel sample holder, and measured using a TRIFT II mass spectrometer (Physical Electronics, Eden Prairie, MN) equipped with a Au primary ion gun. 22 keV Au<sup>+</sup> primary ions were used for analysis. SIMS spectra were measured both in the positive and the negative secondary ion mode with a secondary ion energy of 7 keV.</p></sec><sec id="Sec10"><title><italic>In Vivo</italic> Degradation Study</title><sec id="Sec11"><title>Animal Model</title><p>All experimental protocols and procedures were approved by the local experimental animal welfare committee and conform to national and European regulations for animal experimentation. Eight adult female New Zealand White rabbits of approximately 3.5 kg were purchased from Harlan (Horst, The Netherlands). The animals were housed individually to allow for urine and faeces collection and were fed approximately 100 g complete diet pellets for rabbits daily. Water was supplied <italic>ad libitum</italic>.</p></sec><sec id="Sec12"><title>Tumor Cells</title><p>The VX2 cell line was a kind gift from dr. R.J.J. van Es (department of Oral and Maxillofacial surgery, University Medical Centre Utrecht, Utrecht, The Netherlands) (<xref ref-type="bibr" rid="CR18">18</xref>). The VX2 cells were propagated by subcutaneous passage in the rabbit hind limb. After dissecting the tumor, small portions of viable tumor (2 mm in diameter) were selected for intrahepatic implantation as described by Nijsen <italic>et al</italic>. (<xref ref-type="bibr" rid="CR19">19</xref>).</p></sec><sec id="Sec13"><title>Analgesia, Sedation and Euthanasia</title><p>Analgesia during and 2 days post laparotomy was achieved with carprofen s.c. 4 mg kg<sup>−1</sup> bodyweight. General anaesthesia was induced by ketamine i.m. at 30 mg kg<sup>−1</sup> bodyweight and xylazine i.m. at 4 mg kg<sup>−1</sup> bodyweight. Anaesthesia was maintained by inhalation of isoflurane (1.5–2.0% O<sub>2</sub>/air (1:1)). For post-administration imaging, the animals were sedated using dexmedetomidine at 50 mg kg<sup>−1</sup> and ketamine at 15 mg kg<sup>−1</sup>. The animals were euthanized by intravenous injection of 600 mg pentobarbital.</p></sec><sec id="Sec14"><title>Tumor Implantation</title><p>A subxiphoid laparotomy was performed by ventral mid-line incision, in order to expose the liver lobes. Three tumor pieces were implanted into the left lateral liver lobe with an intravenous catheter (Abbocath−T 16 G, Hospira, Donegal Town, Ireland). After implantation of tumor tissue, the puncture wound in the liver was sealed with tissue glue (Histoacryl, B. Braun, Melsungen, Germany). Thereafter the abdominal muscle layer and skin layers were sutured in separate layers using Vicryl 3.0 (Johnson&Johnson Intl., St-Stevens-Woluwe, Belgium).</p></sec><sec id="Sec15"><title>Dose Preparation</title><p>Neutron irradiation of approximately 60 mg HoAcAcMS for 1 h resulted in an activity of approximately 600 MBq <sup>166</sup>HoAcAcMS. The <sup>166</sup>HoAcAcMS were suspended in 1.2 mL of sterile water containing 2% Pluronic<sup>®</sup> F68 and 10% ethanol abs. to obtain a final concentration of 50 mg mL<sup>−1</sup> (=500 MBq <sup>166</sup>HoAcAcMS mL<sup>−1</sup>). The microspheres were suspended by gentle agitation and repeatedly drawing them up and down with a syringe. Subsequently, approximately 200 μL (=100 MBq or 10 mg <sup>166</sup>HoAcAcMS) was taken up in 29 G insulin syringes (Becton Dickinson Ultra Fine, Breda, the Netherlands), and the activity was measured using a dose calibrator (VDC-404, Veenstra Instruments, Joure, The Netherlands). As a control, a suspension of non-radioactive microspheres was prepared in a similar concentration of microspheres (50 mg mL<sup>−1</sup>). Approximately 200 μL (or 10 mg) was taken up in 29 G insulin syringes.</p></sec><sec id="Sec16"><title>Administration of <sup>166</sup>HoAcAcMS</title><p>Fourteen days post implantation the tumor reached a size of approximately 2 cm<sup>3</sup> and a second subxiphoid laparotomy was performed. Prior to administration of the microspheres, the syringe was agitated vigorously to obtain a homogeneous microsphere suspension. One hundred μl of non-radioactive (=5 mg) or radioactive HoAcAcMS (=50 MBq, which corresponds to 5 mg) suspension was administered intratumorally. The syringes were measured in a dose calibrator before and after administration, to determine the injected amount of activity. Following the intratumoral administration of <sup>166</sup>HoAcAcMS, the animals were subjected to planar gamma scintigraphy (Forte, Philips Medical Systems, Best, The Netherlands) and CT imaging (Brilliance 64, Philips Medical Systems, Best, The Netherlands) to confirm the selective deposition of microspheres in the tumor.</p></sec><sec id="Sec17"><title>Release Profile of the <sup>166</sup>HoAcAcMS</title><p>The urine and faeces of the animals were collected at the same time each day during the first week after administration. Venous blood samples (1 mL) were taken before, just after, 5 days after and 10 days after administration of HoAcAcMS. Blood was centrifuged at 3,000 rpm for 10 min at 4°C and the serum was collected and stored at −20°C for further analysis. The serum levels of alkaline phosphatase (ALP) as an indicator of biliary toxicity, alanine aminotransferase (ALAT), aspartate amino transferase (ASAT) and gamma glutamyltransferase (<italic>γ</italic>-GT) as indicators of hepatocellular toxicity and albumin as indicator for liver function were analysed on a UniCel DxC 800 Clinical System (Beckman Coulter B.V., Woerden, The Netherlands). In addition, the serum holmium levels were measured using inductively coupled plasma−optical emission spectroscopy (ICP OES), as described in section 2.9.</p></sec><sec id="Sec18"><title>Histology</title><p>Rabbits were euthanized 4 weeks after administration of HoAcAcMS. After termination, the lungs, heart, stomach, liver, kidneys, and a femur were excised and fixed in a 4% formalin solution. The tumor bearing liver was dissected and embedded in paraffin. After haematoxylin-eosin staining the liver and tumor tissue was histologically evaluated, to verify the presence of microspheres in the tumor and liver.</p></sec></sec><sec id="Sec19"><title>Determination of Holmium Concentration</title><p>The holmium concentration in buffer, serum, urine, faeces and bone samples was determined using ICP OES. Buffer samples were prepared by diluting 100 μL of supernatant to 5 mL nitric acid (5% <italic>w/v</italic>). Approximately 1 g of urine or faeces sample was destructed overnight using 5 mL nitric acid (30%). After overnight destruction the samples were centrifuged at 4,000 g and 1 mL of supernatant was diluted to 5 ml with nitric acid (5% <italic>w/v</italic>). The bone was separated from the marrow of the femur samples. Approximately 500 mg bone sample was weighed accurately, and was destructed in 4 mL aqua regia (one volume of nitric acid (65% <italic>w/w</italic>) mixed with three volumes hydrochloric acid (36% <italic>w/w</italic>)) under heating to 100°C. After evaporation to dryness, the residue was dissolved in 5 mL nitric acid (5% <italic>w/v</italic>). All samples were measured at three different wavelengths (339.898 nm, 345.600 nm and 347.426 nm respectively) by ICP-OES, using an Optima 4300 (PerkinElmer, Norwalk, USA). Analytical calibration curves from the holmium ICP standard solution were used to assess the precision and accuracy of the measurements.</p></sec></sec><sec id="Sec20"><title>RESULTS AND DISCUSSION</title><p>Microsphere characteristics of the HoAcAcMS used in these experiments were in accordance with previous work (<xref ref-type="bibr" rid="CR12">12</xref>). The microspheres had a holmium load of 45 ± 1%, and a size of 15 μm (97% of the microspheres between 10 and 20 μm) after sieving. As previously observed, no differences in size distribution were observed before and after neutron irradiation, irrespective of irradiation time (<xref ref-type="bibr" rid="CR12">12</xref>).</p><p>The <italic>in vitro</italic> release profile of holmium from the microspheres incubated in phosphate buffer is shown in Fig. <xref rid="Fig1" ref-type="fig">1</xref>. As can be observed, the release of holmium was below 0.5% after 6 months. This is comparable to the holmium release (0.7 ± 0.2%) reported for holmium loaded poly(L-lactic acid) microspheres (HoPLLAMS) in the same buffer (<xref ref-type="bibr" rid="CR16">16</xref>). The HoPLLAMS are considered radiochemically stable and are currently under clinical investigation in a Phase I study for treatment of liver malignancies (<xref ref-type="bibr" rid="CR20">20</xref>).<fig id="Fig1"><label>Fig. 1</label><caption><p>Release of holmium from HoAcAcMS (<italic>diamonds</italic>) and <sup>166</sup>HoAcAcMS irradiated for either 3 h (<italic>triangles</italic>) or 6 h (<italic>squares</italic>) during incubation in phosphate buffer for 6 months. The data are presented as the mean of two measurements.</p></caption><graphic xlink:href="11095_2011_610_Fig1_HTML" id="MO1"></graphic></fig></p><p>Electron microscopic inspection of the HoAcAcMS after incubation in phosphate buffer showed clusters of microspheres amidst buffer. Since the surface charge of the HoAcAcMS is expected to be near neutral (<xref ref-type="bibr" rid="CR21">21</xref>), the microspheres aggregate upon settling due to gravitational forces. The phosphate buffer filled the inter-microsphere spaces which turned into buffer salts upon drying, thereby encapsulating the microspheres. The microspheres remained intact, irrespective of the neutron irradiation time and incubation time (Fig. <xref rid="Fig2" ref-type="fig">2</xref>). The surface of the microspheres was still smooth after 26 weeks in buffer. This finding is in contradistinction to neutron irradiated HoPLLAMS incubated in a similar buffer (<xref ref-type="bibr" rid="CR16">16</xref>) which showed a rough surface after 12 weeks of incubation and started to disintegrate after 24 weeks incubation. This disintegration was attributed to hydrolysis of the poly(L-lactic acid), leading to the formation of holmium lactate (<xref ref-type="bibr" rid="CR16">16</xref>).<fig id="Fig2"><label>Fig. 2</label><caption><p>Scanning electron micrographs of HoAcAcMS and <sup>166</sup>HoAcAcMS after incubation for different times in phosphate buffer. (<bold>a</bold>–<bold>c</bold>) HoAcAcMS, (<bold>a</bold>) after 1 month, (<bold>b</bold>) after 3 months and (<bold>c</bold>) after 6 months of incubation. (<bold>d</bold>–<bold>f</bold>) <sup>166</sup>HoAcAcMS neutron irradiated for 3 h at a neutron flux of 5 × 10<sup>12</sup> n cm<sup>−2</sup> s<sup>−1</sup> (<bold>d</bold>) after 1 month, (<bold>e</bold>) after 3 months and (<bold>f</bold>) after 6 months of incubation. (<bold>g</bold>–<bold>i</bold>) <sup>166</sup>HoAcAcMS neutron irradiated for 6 h at a neutron flux of 5 × 10<sup>12</sup> n cm<sup>−2</sup> s<sup>−1</sup> (<bold>g</bold>) after 1 month, (<bold>h</bold>) after 3 months and (<bold>i</bold>) after 6 months of incubation. The magnification in all images is 1000x, the bar represents 50 μm.</p></caption><graphic xlink:href="11095_2011_610_Fig2_HTML" id="MO2"></graphic></fig></p><p>The HoAcAcMS were subjected to elemental analysis to determine their composition after incubation in phosphate buffer (Table <xref rid="Tab1" ref-type="table">I</xref>). Interestingly, a quantitative exchange of acetylacetonate by phosphate was observed within 4 days of incubation. To confirm the exchange of acetylacetonate by phosphate, IR analysis was performed. The characteristic peaks of holmium acetylacetonate around 1,518 cm<sup>−1</sup> are present in the samples before and after 1 day of incubation in buffer. The intensity of this peak reduces in time and after 1 month it could not be observed anymore. Interestingly, after 4 days a broad peak around 1,100 cm<sup>−1</sup> appeared, which can be attributed to stretching bands of phosphate groups (<xref ref-type="bibr" rid="CR22">22</xref>).<table-wrap id="Tab1"><label>Table I</label><caption><p>Elemental Composition of the HoAcAcMS after Incubation in a 116 mM Phosphate Buffer</p></caption><table frame="hsides" rules="groups"><thead><tr><th rowspan="2"></th><th colspan="2">Day 0<sup>a</sup></th><th colspan="2">Day 1</th><th colspan="2">Day 4</th><th colspan="2">Day 7</th><th colspan="2">Day 30</th><th colspan="2">Day 90</th></tr><tr><th>%<sup>b</sup></th><th>no<sup>c</sup></th><th>%</th><th>no</th><th>%</th><th>no</th><th>%</th><th>no</th><th>%</th><th>no</th><th>%</th><th>no</th></tr></thead><tbody><tr><td>C</td><td char="." align="char">26.4</td><td char="." align="char">7.5</td><td char="." align="char">15.0</td><td char="." align="char">4.4</td><td char="." align="char">1.0</td><td char="." align="char">0.3</td><td char="." align="char">0.6</td><td char="." align="char">0.2</td><td char="." align="char">0.5</td><td char="." align="char">0.1</td><td char="." align="char">0.7</td><td char="." align="char">0.2</td></tr><tr><td>H</td><td char="." align="char">4.0</td><td char="." align="char">13.5</td><td char="." align="char">3.5</td><td char="." align="char">12.0</td><td char="." align="char">1.5</td><td char="." align="char">4.9</td><td char="." align="char">2.2</td><td char="." align="char">7.5</td><td char="." align="char">1.5</td><td char="." align="char">4.5</td><td char="." align="char">1.6</td><td char="." align="char">4.6</td></tr><tr><td>O</td><td char="." align="char">20.3</td><td char="." align="char">4.3</td><td char="." align="char">29.0</td><td char="." align="char">6.3</td><td char="." align="char">35.4</td><td char="." align="char">7.2</td><td char="." align="char">37.9</td><td char="." align="char">8.1</td><td char="." align="char">30.9</td><td char="." align="char">5.7</td><td char="." align="char">30.1</td><td char="." align="char">5.6</td></tr><tr><td>Ho</td><td char="." align="char">48.4</td><td char="." align="char">1.0</td><td char="." align="char">47.2</td><td char="." align="char">1.0</td><td char="." align="char">50.8</td><td char="." align="char">1.0</td><td char="." align="char">48.2</td><td char="." align="char">1.0</td><td char="." align="char">55.5</td><td char="." align="char">1.0</td><td char="." align="char">55.8</td><td char="." align="char">1.0</td></tr><tr><td>P</td><td char="." align="char">0.5</td><td char="." align="char">0.05</td><td char="." align="char">5.2</td><td char="." align="char">0.6</td><td char="." align="char">10.7</td><td char="." align="char">1.1</td><td char="." align="char">10.7</td><td char="." align="char">1.2</td><td char="." align="char">11.3</td><td char="." align="char">1.1</td><td char="." align="char">11.0</td><td char="." align="char">1.0</td></tr><tr><td>Composition<sup>d</sup></td><td colspan="2">Ho<sub>2</sub>(AcAc)<sub>3</sub> * H<sub>2</sub>O</td><td colspan="2">Intermediate</td><td colspan="2">HoPO<sub>4</sub> * 3 H<sub>2</sub>O</td><td colspan="2">HoPO<sub>4</sub> * 3 H<sub>2</sub>O</td><td colspan="2">HoPO<sub>4</sub> * 3 H<sub>2</sub>O</td><td colspan="2">HoPO<sub>4</sub> * 3 H<sub>2</sub>O</td></tr></tbody></table><table-wrap-foot><p><sup>a</sup>data taken from Bult <italic>et al</italic>. (<xref ref-type="bibr" rid="CR12">12</xref>)</p><p><sup>b</sup>results from elemental analysis are the mean of two values</p><p><sup>c</sup>no: the number of atoms, normalized on holmium</p><p><sup>d</sup>Composition: the chemical composition of the particles</p></table-wrap-foot></table-wrap></p><p>TOF-SIMS analysis of HoAcAcMS before and after incubation in buffer showed spherical particles in total ion count mode (Fig. <xref rid="Fig3" ref-type="fig">3</xref>). The resulting mass spectrum showed differences between HoAcAcMS incubated in phosphate buffer and HoAcAcMS before incubation in buffer. The negative ions observed in the HoAcAcMS incubated in buffer were oxygen, chlorine, and phosphorous compounds (<italic>m/z</italic> 31: P<sup>-</sup>; <italic>m/z</italic> 63: PO<sub arrange="stack">2</sub><sup arrange="stack">-</sup>; <italic>m/z</italic> 79 PO<sub arrange="stack">3</sub><sup arrange="stack">-</sup>), which were absent in the spectrum of the non incubated microspheres (Fig. <xref rid="Fig3" ref-type="fig">3</xref>). All negative ions were distributed evenly through the microspheres, indicating a complete exchange of acetylacetonate by phosphate in the microspheres. Holmium (<italic>m/z</italic> 165), and holmium-oxygen (<italic>m/z</italic> 181) was present in both the incubated and non incubated samples. The incubated samples also showed a large peak of sodium (<italic>m/z</italic> 23) from the buffer salts, which was only present at the particle surface. The HoAcAcMS before incubation in phosphate buffer show additional peaks in the positive secondary ion mode at <italic>m/z</italic> 74 and <italic>m/z</italic> 147, which can be attributed to trimethylsilylgroups from polydimethylsiloxane (<xref ref-type="bibr" rid="CR23">23</xref>) that is present on the surface of the carbon sticker.<fig id="Fig3"><label>Fig. 3</label><caption><p>TOF-SIMS spectra from control HoAcAcMS (HoAcAcMS) and buffer incubated HoAcAcMS (HoAcAcMS buffer). The micrographs show total ion count images in the negative (−ve) and the positive (+ve) secondary ion mode. The peaks of interest are indicated with an arrow.</p></caption><graphic xlink:href="11095_2011_610_Fig3_HTML" id="MO3"></graphic></fig></p><p>The results of IR analysis and TOF-SIMS confirm elemental analysis data that phosphate has replaced acetylacetonate after suspension in buffer. This in turn explains the limited release of holmium from the microspheres, since rare earth metal phosphates are practically insoluble in aqueous media; the solubility product of HoPO<sub>4</sub> in water is lower than 10<sup>−25</sup> M (<xref ref-type="bibr" rid="CR24">24</xref>). The exchange of acetylacetonate by phosphate, without changing the surface morphology, is a so called <italic>chimie douce</italic> reaction (<xref ref-type="bibr" rid="CR25">25</xref>). <italic>Chimie douce</italic> reactions are topotactic meaning that the products retain the precursor geometry, and usually take place under mild conditions. Beta diketones, like acetylacetonate, are prone to these types of reactions (<xref ref-type="bibr" rid="CR26">26</xref>,<xref ref-type="bibr" rid="CR27">27</xref>).</p><p>The <italic>in vivo</italic> stability study was carried out to determine the <italic>in vivo</italic> implications of the results obtained in the <italic>in vitro</italic> experiment. Radioactive and cold HoAcAcMS were administered intratumorally to VX2 carcinoma-bearing rabbits. All animals recovered from anaesthesia, and the animals received 40 ± 10 MBq <sup>166</sup>HoAcAcMS, corresponding to 4 ± 1 mg microspheres intratumorally. Approximately 80% of the intended dose was administered, which was attributed to the premature settling of the microspheres in the syringes. Nuclear imaging and CT imaging showed a selective administration of microspheres in the tumor (Fig. <xref rid="Fig4" ref-type="fig">4</xref>). One animal showed uptake of activity in the lungs (approximately 5% of the injected dose) apart from uptake in the tumor, due to the inadvertent delivery of <sup>166</sup>HoAcAcMS in a blood vessel surrounding the tumor artery. This animal however, completed the experiment in good health. The inadvertent delivery of activity can be avoided in humans by administering the microspheres under ultrasound guidance, making it easier to distinguish tumor tissue from blood vessels and liver tissue (<xref ref-type="bibr" rid="CR6">6</xref>).<fig id="Fig4"><label>Fig. 4</label><caption><p>(<bold>a</bold>) Schematic outline of rabbit anatomy on X-ray CT image (L=liver, T=tumor, S=stomach, I=intestine). (<bold>b</bold>) X-ray CT image of rabbit before intratumoral administration of <sup>166</sup>HoAcAcMS showing the tumor, (<bold>c</bold>) X-ray CT image of rabbit after intratumoral administration of <sup>166</sup>HoAcAcMS showing the selective deposition in the tumor as white artifacts, (<bold>d</bold>) X-ray CT image 4 weeks after administration, before termination, the white area in the tumor is still present as a cluster, although the shape has changed.</p></caption><graphic xlink:href="11095_2011_610_Fig4_HTML" id="MO4"></graphic></fig></p><p>The excretion of holmium after intratumoral administration of HoAcAcMS to the blood, urine and the faeces was below the detection limit in all samples (0.1 ppm for Ho). Suzuki and co-workers found that release of free holmium (Ho<sup>3+</sup>) to the urine and faeces occurred rapidly after local intrahepatic administration of the <italic>in-situ</italic> gel forming complex holmium-166-chitosan (<xref ref-type="bibr" rid="CR28">28</xref>). Therefore it can be concluded that Ho<sup>3+</sup> was not released from the HoAcAcMS, and that the microspheres retained their integrity as confirmed by histology.</p><p>Although the results from the <italic>in vitro</italic> and the <italic>in vivo</italic> stability study show a minimal to no release of holmium from the HoAcAcMS, the <italic>in vivo</italic> data cannot be related to the chimie douce reaction found <italic>in vitro</italic>. The phosphate concentration (116 mM) used for the <italic>in vitro</italic> experiment is substantially higher than the phosphate concentration in human serum (around 1.5 mM (<xref ref-type="bibr" rid="CR29">29</xref>)). The high phosphate concentration <italic>in vitro</italic> was required to keep the pH stable throughout the experiment, which, however, might accelerate the exchange of acetylacetonate by phosphate. The exchange of acetylacetonate by phosphate could not be established <italic>in vivo</italic> since paraffin interfered with the TOF-SIMS measurements, nonetheless, the presence of intact microspheres and the absence of holmium in urine, faeces and blood show that the HoAcAcMS are stable <italic>in vivo</italic>. The identification of the chemical structure of the HoAcAcMS after intratumoral administration requires further research.</p><p>A transient rise in ASAT was observed after the administration of the radioactive microspheres, which can be attributed to the subxiphoid manipulation of liver tissue, and radiation effects of the <sup>166</sup>HoAcAcMS. No other elevations from baseline levels or differences in serum enzyme levels were found between the radioactive and non-radioactive group (see Fig. <xref rid="Fig5" ref-type="fig">5</xref>). ASAT and ALAT are markers for short term liver damage, whereas gamma GT and Alkaline Phosphatase are a measure for long-term (chronic) liver damage. Albumin levels are a marker for the liver function, since the liver is the main site for albumin production (<xref ref-type="bibr" rid="CR30">30</xref>). The transient rise in ASAT was previously observed after transcatheter radioembolization with both <sup>90</sup>Y microspheres in human liver cancer patients (<xref ref-type="bibr" rid="CR31">31</xref>) and <sup>166</sup>HoPLLAMS in healthy pigs (<xref ref-type="bibr" rid="CR30">30</xref>). This transient rise was attributed to the manipulation of liver tissue and radiation effects of the microspheres (<xref ref-type="bibr" rid="CR30">30</xref>,<xref ref-type="bibr" rid="CR31">31</xref>). No changes in serum enzyme levels or albumin levels were observed in the present study, an indication that the liver function was neither impaired by the tumor, nor intratumoral administration of either HoAcAcMS or <sup>166</sup>HoAcAcMS.<fig id="Fig5"><label>Fig. 5</label><caption><p>Serum enzyme levels (ASAT, ALAT, γ-GT, ALP and albumin) of rabbits that received non-radioactive HoAcAcMS (<italic>solid line</italic>) and radioactive <sup>166</sup>HoAcAcMS (<italic>dotted line</italic>) in time. Bars represent SD.</p></caption><graphic xlink:href="11095_2011_610_Fig5_HTML" id="MO5"></graphic></fig></p><p>Histological evaluation of liver sections showed intact microspheres amidst necrotic tissue both for HoAcAcMS and <sup>166</sup>HoAcAcMS (Fig. <xref rid="Fig6" ref-type="fig">6</xref>), which is in agreement with the results from the <italic>in vitro</italic> stability study showing intact HoAcAcMS after 6 months incubation in buffer (Fig. <xref rid="Fig2" ref-type="fig">2</xref>). Both the non-radioactive HoAcAcMS and the <sup>166</sup>HoAcAcMS are surrounded by necrotic tissue. The VX2 tumor is a rapid-growing tumor, with a viable rim and a necrotic core. Therefore, the HoAcAcMS are surrounded by necrotic tissue (Fig. <xref rid="Fig6" ref-type="fig">6a</xref>). Radiation damage from the <sup>166</sup>HoAcAcMS resulted in the necrotic tissue surrounding the cluster of microspheres (Fig. <xref rid="Fig6" ref-type="fig">6b</xref>).<fig id="Fig6"><label>Fig. 6</label><caption><p>Light micrographs at 400x magnification of a H&E stained section of tumor tissue showing microspheres amidst necrotic tissue. (<bold>a</bold>) non-radioactive HoAcAcMS. (<bold>b</bold>) <sup>166</sup>HoAcAcMS. The microspheres in red are clustered amidst necrotic tissue. The bar represents 20 μm.</p></caption><graphic xlink:href="11095_2011_610_Fig6_HTML" id="MO6"></graphic></fig></p><p>Possible release of holmium from the HoAcAcMS was determined by measuring the holmium content in bone. Holmium is a bone seeking element, and it has been shown that within 4 days after intravenous administration of holmium nitrate, approximately 55% of the injected dose accumulates in bone (<xref ref-type="bibr" rid="CR32">32</xref>). Therefore, if holmium (Ho<sup>3+</sup>) would be released from the HoAcAcMS <italic>in vivo</italic>, the holmium levels in bone would be elevated. In accordance with the holmium levels in urine and faeces, the holmium levels in all bone samples were below the detection limit and it can be concluded that no release of holmium from the HoAcAcMS has occurred.</p></sec><sec id="Sec21"><title>CONCLUSION</title><p>This paper demonstrates the stability of HoAcAcMS, both <italic>in vitro</italic> and <italic>in vivo</italic>. The <italic>in vitro</italic> release was approximately 0.5% after 6 months of incubation in buffer. The microspheres remained spherical, and a HoPO<sub>4</sub> complex was formed while the surface morphology was retained. No release of holmium was observed after the intratumoral injection of HoAcAcMS in VX2 tumor-bearing rabbits, and the microspheres remained intact in tumor tissue for 1 month. The results confirm the potential of these microspheres as a novel intratumoral radioablation device.</p></sec></body><back><ack><title>ACKNOWLEDGMENTS & DISCLOSURES</title><p>Financial support by the Dutch Technology Foundation STW, under grant 06069 is gratefully acknowledged. Dr. G.C. Krijger and ms. M.J.J. Koster-Ammerlaan are acknowledged for performing the neutron irradiations. Ms. H.M. de Bruin, Mr. N.J.M. Attevelt and Mr. H.W.G. Vosmeer are gratefully acknowledged for biotechnical assistance. The authors would like to thank prof. dr. K.P. de Jong for valuable discussions. Dr. W.H. Müller and Mr. C.J.M.M. 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