In Vivo Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition
Identifieur interne : 000270 ( Main/Exploration ); précédent : 000269; suivant : 000271In Vivo Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition
Auteurs : Yoke Chin Chai ; Liesbet Geris ; Johanna Bolander ; Grzegorz Pyka ; Simon Van Bael ; Frank P. Luyten ; Jan SchrootenSource :
- BioResearch Open Access [ 2164-7844 ] ; 2014.
Abstract
Functionalization of tissue engineering scaffolds with
Url:
DOI: 10.1089/biores.2014.0050
PubMed: 25469312
PubMed Central: 4245878
Affiliations:
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Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition</title>
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<author><name sortKey="Geris, Liesbet" sort="Geris, Liesbet" uniqKey="Geris L" first="Liesbet" last="Geris">Liesbet Geris</name>
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<author><name sortKey="Van Bael, Simon" sort="Van Bael, Simon" uniqKey="Van Bael S" first="Simon" last="Van Bael">Simon Van Bael</name>
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<author><name sortKey="Luyten, Frank P" sort="Luyten, Frank P" uniqKey="Luyten F" first="Frank P." last="Luyten">Frank P. Luyten</name>
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<author><name sortKey="Schrooten, Jan" sort="Schrooten, Jan" uniqKey="Schrooten J" first="Jan" last="Schrooten">Jan Schrooten</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In Vivo</italic>
Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition</title>
<author><name sortKey="Chai, Yoke Chin" sort="Chai, Yoke Chin" uniqKey="Chai Y" first="Yoke Chin" last="Chai">Yoke Chin Chai</name>
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<author><name sortKey="Geris, Liesbet" sort="Geris, Liesbet" uniqKey="Geris L" first="Liesbet" last="Geris">Liesbet Geris</name>
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<author><name sortKey="Bolander, Johanna" sort="Bolander, Johanna" uniqKey="Bolander J" first="Johanna" last="Bolander">Johanna Bolander</name>
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<author><name sortKey="Pyka, Grzegorz" sort="Pyka, Grzegorz" uniqKey="Pyka G" first="Grzegorz" last="Pyka">Grzegorz Pyka</name>
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<author><name sortKey="Van Bael, Simon" sort="Van Bael, Simon" uniqKey="Van Bael S" first="Simon" last="Van Bael">Simon Van Bael</name>
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<author><name sortKey="Luyten, Frank P" sort="Luyten, Frank P" uniqKey="Luyten F" first="Frank P." last="Luyten">Frank P. Luyten</name>
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<author><name sortKey="Schrooten, Jan" sort="Schrooten, Jan" uniqKey="Schrooten J" first="Jan" last="Schrooten">Jan Schrooten</name>
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<series><title level="j">BioResearch Open Access</title>
<idno type="ISSN">2164-7844</idno>
<idno type="eISSN">2164-7860</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>Functionalization of tissue engineering scaffolds with <italic>in vitro</italic>
–generated bone-like extracellular matrix (ECM) represents an effective biomimetic approach to promote osteogenic differentiation of stem cells <italic>in vitro</italic>
. However, the bone-forming capacity of these constructs (seeded with or without cells) is so far not apparent. In this study, we aimed at developing a mineralizing culture condition to biofunctionalize three-dimensional (3D) porous scaffolds with highly mineralized ECM in order to produce devitalized, osteoinductive mineralized carriers for human periosteal-derived progenitors (hPDCs). For this, three medium formulations [i.e., growth medium only (BM1), with ascorbic acid (BM2), and with ascorbic acid and dexamethasone (BM3)] supplemented with calcium (Ca<sup>2+</sup>
) and phosphate (PO<sub>4</sub>
<sup>3−</sup>
) ions simultaneously as mineralizing source were investigated. The results showed that, besides the significant impacts on enhancing cell proliferation (the highest in BM3 condition), the formulated mineralizing media differentially regulated the osteochondro-related gene markers in a medium-dependent manner (e.g., significant upregulation of <italic>BMP2</italic>
, bone sialoprotein, osteocalcin, and <italic>Wnt5a</italic>
in BM2 condition). This has resulted in distinguished cell populations that were identifiable by specific gene signatures as demonstrated by the principle component analysis. Through devitalization, mineralized carriers with apatite crystal structures unique to each medium condition (by X-ray diffraction and SEM analysis) were obtained. Quantitatively, BM3 condition produced carriers with the highest mineral and collagen contents as well as human-specific VEGF proteins, followed by BM2 and BM1 conditions. Encouragingly, all mineralized carriers (after reseeded with hPDCs) induced bone formation after 8 weeks of subcutaneous implantation in nude mice models, with BM2-carriers inducing the highest bone volume, and the lowest in the BM3 condition (as quantitated by nano-computed tomography [nano-CT]). Histological analysis revealed different bone formation patterns, either bone ossicles containing bone marrow surrounding the scaffold struts (in BM2) or bone apposition directly on the struts' surface (in BM1 and BM3). In conclusion, we have presented experimental data on the feasibility to produce devitalized osteoinductive mineralized carriers by functionalizing 3D porous scaffolds with an <italic>in vitro</italic>
cell-made mineralized matrix under the mineralizing culture conditions.</p>
</div>
</front>
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<tree><noCountry><name sortKey="Bolander, Johanna" sort="Bolander, Johanna" uniqKey="Bolander J" first="Johanna" last="Bolander">Johanna Bolander</name>
<name sortKey="Chai, Yoke Chin" sort="Chai, Yoke Chin" uniqKey="Chai Y" first="Yoke Chin" last="Chai">Yoke Chin Chai</name>
<name sortKey="Geris, Liesbet" sort="Geris, Liesbet" uniqKey="Geris L" first="Liesbet" last="Geris">Liesbet Geris</name>
<name sortKey="Luyten, Frank P" sort="Luyten, Frank P" uniqKey="Luyten F" first="Frank P." last="Luyten">Frank P. Luyten</name>
<name sortKey="Pyka, Grzegorz" sort="Pyka, Grzegorz" uniqKey="Pyka G" first="Grzegorz" last="Pyka">Grzegorz Pyka</name>
<name sortKey="Schrooten, Jan" sort="Schrooten, Jan" uniqKey="Schrooten J" first="Jan" last="Schrooten">Jan Schrooten</name>
<name sortKey="Van Bael, Simon" sort="Van Bael, Simon" uniqKey="Van Bael S" first="Simon" last="Van Bael">Simon Van Bael</name>
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