In Vivo Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition
Identifieur interne : 000338 ( Pmc/Curation ); précédent : 000337; suivant : 000339In Vivo Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition
Auteurs : Yoke Chin Chai ; Liesbet Geris ; Johanna Bolander ; Grzegorz Pyka ; Simon Van Bael ; Frank P. Luyten ; Jan SchrootenSource :
- BioResearch Open Access [ 2164-7844 ] ; 2014.
Abstract
Functionalization of tissue engineering scaffolds with
Url:
DOI: 10.1089/biores.2014.0050
PubMed: 25469312
PubMed Central: 4245878
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000338
Links to Exploration step
PMC:4245878Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In Vivo</italic> Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition</title><author><name sortKey="Chai, Yoke Chin" sort="Chai, Yoke Chin" uniqKey="Chai Y" first="Yoke Chin" last="Chai">Yoke Chin Chai</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Geris, Liesbet" sort="Geris, Liesbet" uniqKey="Geris L" first="Liesbet" last="Geris">Liesbet Geris</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Bolander, Johanna" sort="Bolander, Johanna" uniqKey="Bolander J" first="Johanna" last="Bolander">Johanna Bolander</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Pyka, Grzegorz" sort="Pyka, Grzegorz" uniqKey="Pyka G" first="Grzegorz" last="Pyka">Grzegorz Pyka</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author><author><name sortKey="Van Bael, Simon" sort="Van Bael, Simon" uniqKey="Van Bael S" first="Simon" last="Van Bael">Simon Van Bael</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff6"></nlm:aff></affiliation></author><author><name sortKey="Luyten, Frank P" sort="Luyten, Frank P" uniqKey="Luyten F" first="Frank P." last="Luyten">Frank P. Luyten</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Schrooten, Jan" sort="Schrooten, Jan" uniqKey="Schrooten J" first="Jan" last="Schrooten">Jan Schrooten</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25469312</idno><idno type="pmc">4245878</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245878</idno><idno type="RBID">PMC:4245878</idno><idno type="doi">10.1089/biores.2014.0050</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000338</idno><idno type="wicri:Area/Pmc/Curation">000338</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In Vivo</italic> Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition</title><author><name sortKey="Chai, Yoke Chin" sort="Chai, Yoke Chin" uniqKey="Chai Y" first="Yoke Chin" last="Chai">Yoke Chin Chai</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Geris, Liesbet" sort="Geris, Liesbet" uniqKey="Geris L" first="Liesbet" last="Geris">Liesbet Geris</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Bolander, Johanna" sort="Bolander, Johanna" uniqKey="Bolander J" first="Johanna" last="Bolander">Johanna Bolander</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Pyka, Grzegorz" sort="Pyka, Grzegorz" uniqKey="Pyka G" first="Grzegorz" last="Pyka">Grzegorz Pyka</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author><author><name sortKey="Van Bael, Simon" sort="Van Bael, Simon" uniqKey="Van Bael S" first="Simon" last="Van Bael">Simon Van Bael</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff6"></nlm:aff></affiliation></author><author><name sortKey="Luyten, Frank P" sort="Luyten, Frank P" uniqKey="Luyten F" first="Frank P." last="Luyten">Frank P. Luyten</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Schrooten, Jan" sort="Schrooten, Jan" uniqKey="Schrooten J" first="Jan" last="Schrooten">Jan Schrooten</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author></analytic><series><title level="j">BioResearch Open Access</title><idno type="ISSN">2164-7844</idno><idno type="eISSN">2164-7860</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Functionalization of tissue engineering scaffolds with <italic>in vitro</italic>–generated bone-like extracellular matrix (ECM) represents an effective biomimetic approach to promote osteogenic differentiation of stem cells <italic>in vitro</italic>. However, the bone-forming capacity of these constructs (seeded with or without cells) is so far not apparent. In this study, we aimed at developing a mineralizing culture condition to biofunctionalize three-dimensional (3D) porous scaffolds with highly mineralized ECM in order to produce devitalized, osteoinductive mineralized carriers for human periosteal-derived progenitors (hPDCs). For this, three medium formulations [i.e., growth medium only (BM1), with ascorbic acid (BM2), and with ascorbic acid and dexamethasone (BM3)] supplemented with calcium (Ca<sup>2+</sup>) and phosphate (PO<sub>4</sub><sup>3−</sup>) ions simultaneously as mineralizing source were investigated. The results showed that, besides the significant impacts on enhancing cell proliferation (the highest in BM3 condition), the formulated mineralizing media differentially regulated the osteochondro-related gene markers in a medium-dependent manner (e.g., significant upregulation of <italic>BMP2</italic>, bone sialoprotein, osteocalcin, and <italic>Wnt5a</italic> in BM2 condition). This has resulted in distinguished cell populations that were identifiable by specific gene signatures as demonstrated by the principle component analysis. Through devitalization, mineralized carriers with apatite crystal structures unique to each medium condition (by X-ray diffraction and SEM analysis) were obtained. Quantitatively, BM3 condition produced carriers with the highest mineral and collagen contents as well as human-specific VEGF proteins, followed by BM2 and BM1 conditions. Encouragingly, all mineralized carriers (after reseeded with hPDCs) induced bone formation after 8 weeks of subcutaneous implantation in nude mice models, with BM2-carriers inducing the highest bone volume, and the lowest in the BM3 condition (as quantitated by nano-computed tomography [nano-CT]). Histological analysis revealed different bone formation patterns, either bone ossicles containing bone marrow surrounding the scaffold struts (in BM2) or bone apposition directly on the struts' surface (in BM1 and BM3). In conclusion, we have presented experimental data on the feasibility to produce devitalized osteoinductive mineralized carriers by functionalizing 3D porous scaffolds with an <italic>in vitro</italic> cell-made mineralized matrix under the mineralizing culture conditions.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ma, Px" uniqKey="Ma P">PX Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ingber, De" uniqKey="Ingber D">DE Ingber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grayson, Wl" uniqKey="Grayson W">WL Grayson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benders, Ke" uniqKey="Benders K">KE Benders</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Badylak, Sf" uniqKey="Badylak S">SF Badylak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arenas Herrera, Je" uniqKey="Arenas Herrera J">JE Arenas-Herrera</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ott, Hc" uniqKey="Ott H">HC Ott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosos, Gi" uniqKey="Drosos G">GI Drosos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dinopoulos, Ht" uniqKey="Dinopoulos H">HT Dinopoulos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harakas, Nk" uniqKey="Harakas N">NK Harakas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hinze, Mc" uniqKey="Hinze M">MC Hinze</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Urist, Mr" uniqKey="Urist M">MR Urist</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Datta, N" uniqKey="Datta N">N Datta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pham, Qp" uniqKey="Pham Q">QP Pham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Antebi, B" uniqKey="Antebi B">B Antebi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pham, Qp" uniqKey="Pham Q">QP Pham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sadr, N" uniqKey="Sadr N">N Sadr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boonrungsiman, S" uniqKey="Boonrungsiman S">S Boonrungsiman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Golub, Ee" uniqKey="Golub E">EE Golub</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Colfen, H" uniqKey="Colfen H">H Colfen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thula, Tt" uniqKey="Thula T">TT Thula</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barrere, F" uniqKey="Barrere F">F Barrere</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bigi, A" uniqKey="Bigi A">A Bigi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lopez Heredia, Ma" uniqKey="Lopez Heredia M">MA Lopez-Heredia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miao, S" uniqKey="Miao S">S Miao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsui, Yc" uniqKey="Tsui Y">YC Tsui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fu, Q" uniqKey="Fu Q">Q Fu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nguyen, Hq" uniqKey="Nguyen H">HQ Nguyen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Landis, Wj" uniqKey="Landis W">WJ Landis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, H" uniqKey="Yuan H">H Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chai, Yc" uniqKey="Chai Y">YC Chai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chai, Yc" uniqKey="Chai Y">YC Chai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Bael, S" uniqKey="Van Bael S">S Van Bael</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eyckmans, J" uniqKey="Eyckmans J">J Eyckmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chai, Yc" uniqKey="Chai Y">YC Chai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bleek, K" uniqKey="Bleek K">K Bleek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sittichockechaiwut, A" uniqKey="Sittichockechaiwut A">A Sittichockechaiwut</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chai, Yc" uniqKey="Chai Y">YC Chai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roberts, Sj" uniqKey="Roberts S">SJ Roberts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bradt, Jh" uniqKey="Bradt J">JH Bradt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mcculloch, Ca" uniqKey="Mcculloch C">CA McCulloch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roberts, Sj" uniqKey="Roberts S">SJ Roberts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ganss, B" uniqKey="Ganss B">B Ganss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Malaval, L" uniqKey="Malaval L">L Malaval</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zilberberg, L" uniqKey="Zilberberg L">L Zilberberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Komori, T" uniqKey="Komori T">T Komori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chatterjea, A" uniqKey="Chatterjea A">A Chatterjea</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Piters, E" uniqKey="Piters E">E Piters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takada, I" uniqKey="Takada I">I Takada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Topol, L" uniqKey="Topol L">L Topol</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sapir Koren, R" uniqKey="Sapir Koren R">R Sapir-Koren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zelzer, E" uniqKey="Zelzer E">E Zelzer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, H" uniqKey="Lu H">H Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, M" uniqKey="He M">M He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Crapo, Pm" uniqKey="Crapo P">PM Crapo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haykal, S" uniqKey="Haykal S">S Haykal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Langenbach, F" uniqKey="Langenbach F">F Langenbach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nudelman, F" uniqKey="Nudelman F">F Nudelman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Serafim, A" uniqKey="Serafim A">A Serafim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kerchkhofs, G" uniqKey="Kerchkhofs G">G Kerchkhofs</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thimm, Bw" uniqKey="Thimm B">BW Thimm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wozney, Jm" uniqKey="Wozney J">JM Wozney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wozney, Jm" uniqKey="Wozney J">JM Wozney</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biores Open Access</journal-id><journal-id journal-id-type="iso-abbrev">Biores Open Access</journal-id><journal-id journal-id-type="publisher-id">biores</journal-id><journal-title-group><journal-title>BioResearch Open Access</journal-title></journal-title-group><issn pub-type="ppub">2164-7844</issn><issn pub-type="epub">2164-7860</issn><publisher><publisher-name>Mary Ann Liebert, Inc.</publisher-name><publisher-loc>140 Huguenot Street, 3rd FloorNew Rochelle, NY 10801USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25469312</article-id><article-id pub-id-type="pmc">4245878</article-id><article-id pub-id-type="publisher-id">10.1089/biores.2014.0050</article-id><article-id pub-id-type="doi">10.1089/biores.2014.0050</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Research Articles</subject></subj-group></article-categories><title-group><article-title><italic>In Vivo</italic> Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chai</surname><given-names>Yoke Chin</given-names></name><xref ref-type="aff" rid="aff1"><sup>1,</sup></xref><xref ref-type="aff" rid="aff2"><sup>2,</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Geris</surname><given-names>Liesbet</given-names></name><xref ref-type="aff" rid="aff3"><sup>3,</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bolander</surname><given-names>Johanna</given-names></name><xref ref-type="aff" rid="aff1"><sup>1,</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pyka</surname><given-names>Grzegorz</given-names></name><xref ref-type="aff" rid="aff3"><sup>3,</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Van Bael</surname><given-names>Simon</given-names></name><xref ref-type="aff" rid="aff3"><sup>3,</sup></xref><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Luyten</surname><given-names>Frank P.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1,</sup></xref><xref ref-type="aff" rid="aff3"><sup>3,</sup></xref><xref ref-type="author-notes" rid="fn1"><sup>*</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Schrooten</surname><given-names>Jan</given-names></name><xref ref-type="aff" rid="aff3"><sup>3,</sup></xref><xref ref-type="aff" rid="aff5"><sup>5,</sup></xref><xref ref-type="author-notes" rid="fn1"><sup>*</sup></xref></contrib><aff id="aff1"><label><sup>1</sup></label>Tissue Engineering Laboratory,<institution>Skeletal Biology and Engineering Research Center</institution>, KU Leuven, Leuven,<country>Belgium</country>.</aff><aff id="aff2"><label><sup>2</sup></label>Department of Biomedical Engineering, Faculty of Engineering,<institution>University of Malaya</institution>, Kuala Lumpur,<country>Malaysia</country>.</aff><aff id="aff3"><label><sup>3</sup></label>Prometheus, Division of Skeletal Tissue Engineering,<institution>KU Leuven</institution>, Leuven,<country>Belgium</country>.</aff><aff id="aff4"><label><sup>4</sup></label>Biomechanics Research Unit,<institution>University of Liege</institution>, Liege,<country>Belgium</country>.</aff><aff id="aff5"><label><sup>5</sup></label>Department of Materials Engineering,<institution>KU Leuven</institution>, Heverlee,<country>Belgium</country>.</aff><aff id="aff6"><label><sup>6</sup></label>Division of Production Engineering, Machine Design and Automation, Department of Mechanical Engineering,<institution>KU Leuven</institution>, Heverlee,<country>Belgium</country>.</aff></contrib-group><author-notes><fn id="fn1" fn-type="eqaul"><label><sup>*</sup></label><p>Shared senior authorship: Jan Schrooten and Frank P. Luyten.</p></fn><corresp><addr-line>Address correspondence to:</addr-line><addr-line><italic>Jan Schrooten, PhD</italic></addr-line><addr-line><italic>Prometheus</italic></addr-line><addr-line><italic>Division of Skeletal Tissue Engineering</italic></addr-line><addr-line><italic>Department of Materials Engineering</italic></addr-line><institution><italic>KU Leuven</italic></institution><addr-line><italic>Kasteelpark Arenberg 44</italic></addr-line><addr-line><italic>Bus 2450</italic></addr-line><addr-line><italic>3001 Heverlee</italic></addr-line><country>Belgium</country><break></break><italic>E-mail:</italic><email xlink:href="mailto:jan.schrooten@mtm.kuleuven.be">jan.schrooten@mtm.kuleuven.be</email></corresp></author-notes><pub-date pub-type="ppub"><day>01</day><month>12</month><year>2014</year><pmc-comment>string-date: December 2014</pmc-comment>
</pub-date><pub-date pub-type="pmc-release"><day>01</day><month>12</month><year>2014</year><pmc-comment>string-date: December 2014</pmc-comment>
</pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>3</volume><issue>6</issue><fpage>265</fpage><lpage>277</lpage><permissions><copyright-statement>Copyright 2014, Mary Ann Liebert, Inc.</copyright-statement><copyright-year>2014</copyright-year></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="biores.2014.0050.pdf"></self-uri><abstract><title>Abstract</title><p>Functionalization of tissue engineering scaffolds with <italic>in vitro</italic>–generated bone-like extracellular matrix (ECM) represents an effective biomimetic approach to promote osteogenic differentiation of stem cells <italic>in vitro</italic>. However, the bone-forming capacity of these constructs (seeded with or without cells) is so far not apparent. In this study, we aimed at developing a mineralizing culture condition to biofunctionalize three-dimensional (3D) porous scaffolds with highly mineralized ECM in order to produce devitalized, osteoinductive mineralized carriers for human periosteal-derived progenitors (hPDCs). For this, three medium formulations [i.e., growth medium only (BM1), with ascorbic acid (BM2), and with ascorbic acid and dexamethasone (BM3)] supplemented with calcium (Ca<sup>2+</sup>) and phosphate (PO<sub>4</sub><sup>3−</sup>) ions simultaneously as mineralizing source were investigated. The results showed that, besides the significant impacts on enhancing cell proliferation (the highest in BM3 condition), the formulated mineralizing media differentially regulated the osteochondro-related gene markers in a medium-dependent manner (e.g., significant upregulation of <italic>BMP2</italic>, bone sialoprotein, osteocalcin, and <italic>Wnt5a</italic> in BM2 condition). This has resulted in distinguished cell populations that were identifiable by specific gene signatures as demonstrated by the principle component analysis. Through devitalization, mineralized carriers with apatite crystal structures unique to each medium condition (by X-ray diffraction and SEM analysis) were obtained. Quantitatively, BM3 condition produced carriers with the highest mineral and collagen contents as well as human-specific VEGF proteins, followed by BM2 and BM1 conditions. Encouragingly, all mineralized carriers (after reseeded with hPDCs) induced bone formation after 8 weeks of subcutaneous implantation in nude mice models, with BM2-carriers inducing the highest bone volume, and the lowest in the BM3 condition (as quantitated by nano-computed tomography [nano-CT]). Histological analysis revealed different bone formation patterns, either bone ossicles containing bone marrow surrounding the scaffold struts (in BM2) or bone apposition directly on the struts' surface (in BM1 and BM3). In conclusion, we have presented experimental data on the feasibility to produce devitalized osteoinductive mineralized carriers by functionalizing 3D porous scaffolds with an <italic>in vitro</italic> cell-made mineralized matrix under the mineralizing culture conditions.</p></abstract><kwd-group kwd-group-type="author"><title><bold>Key words:</bold> </title><kwd>biomineralization</kwd><kwd>bone regeneration</kwd><kwd>devitalization</kwd><kwd>mineralized extracellular matrix</kwd><kwd>osteoinductive carriers</kwd></kwd-group><counts><fig-count count="7"></fig-count><table-count count="2"></table-count><ref-count count="65"></ref-count><page-count count="13"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000338 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000338 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:4245878
|texte= In Vivo Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:25469312" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a OpenAccessBelV2
| This area was generated with Dilib version V0.6.25. |  |