Genetic engineering of rice capable of synthesizing fructans and enhancing chilling tolerance
Identifieur interne : 001467 ( Istex/Curation ); précédent : 001466; suivant : 001468Genetic engineering of rice capable of synthesizing fructans and enhancing chilling tolerance
Auteurs : Akira Kawakami [Japon] ; Yutaka Sato [Japon] ; Midori Yoshida [Japon]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2008-03-03.
Abstract
Fructans are water-soluble fructose oligomers and polymers that are based on sucrose, and have been implicated in protecting plants against water stress. Rice (Oryza sativa L.) is highly sensitive to chilling temperatures, and is not able to synthesize fructans. Two wheat fructan-synthesizing enzymes, sucrose:sucrose 1-fructosyltransferase, encoded by wft2, or sucrose:fructan 6-fructosyltransferase, encoded by wft1, were introduced into rice plants, and rice transformants that accumulate fructans were successfully obtained. The mature leaf blades of transgenic rice lines with wft2 or wft1 accumulated 16.2 mg g1 FW of oligo- and polysaccharides mainly composed of inulin oligomers of more than DP7, and 3.7 mg g1 FW of oligo- and polysaccharides, mainly composed of phlein oligomers of more than DP15, respectively. The transgenic rice seedlings with wft2 accumulated significantly higher concentrations of oligo- and polysaccharides than non-transgenic rice seedlings, and exhibited enhanced chilling tolerance. The oligo- and polysaccharide concentrations of seedlings expressing wft1 were obviously lower than those of lines expressing wft2, and no correlation between oligo- and polysaccharide concentrations and chilling tolerance was detected in wft1-expressing rice lines. The results suggest that transgenic rice lines expressing wheat-derived fructosyltransferase genes accumulated large amounts of fructans in mature leaf blades and exhibited enhanced chilling tolerance at the seedling stage. This is the first report owing that fructan accumulation enhanced tolerance to non-freezing low temperatures.
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DOI: 10.1093/jxb/erm367
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Genetic engineering of rice capable of synthesizing fructans and enhancing chilling tolerance</title><author><name sortKey="Kawakami, Akira" sort="Kawakami, Akira" uniqKey="Kawakami A" first="Akira" last="Kawakami">Akira Kawakami</name><affiliation wicri:level="1"><mods:affiliation>E-mail: akirak@affrc.go.jp</mods:affiliation><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Sato, Yutaka" sort="Sato, Yutaka" uniqKey="Sato Y" first="Yutaka" last="Sato">Yutaka Sato</name><affiliation wicri:level="1"><mods:affiliation>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555</wicri:regionArea></affiliation></author><author><name sortKey="Yoshida, Midori" sort="Yoshida, Midori" uniqKey="Yoshida M" first="Midori" last="Yoshida">Midori Yoshida</name><affiliation wicri:level="1"><mods:affiliation>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7C5E9F2151B3619719CDA062907E775525236B54</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1093/jxb/erm367</idno><idno type="url">https://api.istex.fr/document/7C5E9F2151B3619719CDA062907E775525236B54/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001514</idno><idno type="wicri:Area/Istex/Curation">001467</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Genetic engineering of rice capable of synthesizing fructans and enhancing chilling tolerance</title><author><name sortKey="Kawakami, Akira" sort="Kawakami, Akira" uniqKey="Kawakami A" first="Akira" last="Kawakami">Akira Kawakami</name><affiliation wicri:level="1"><mods:affiliation>E-mail: akirak@affrc.go.jp</mods:affiliation><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Sato, Yutaka" sort="Sato, Yutaka" uniqKey="Sato Y" first="Yutaka" last="Sato">Yutaka Sato</name><affiliation wicri:level="1"><mods:affiliation>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555</wicri:regionArea></affiliation></author><author><name sortKey="Yoshida, Midori" sort="Yoshida, Midori" uniqKey="Yoshida M" first="Midori" last="Yoshida">Midori Yoshida</name><affiliation wicri:level="1"><mods:affiliation>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Hokkaido 062-8555</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2008-03-03">2008-03-03</date><biblScope unit="volume">59</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="793">793</biblScope><biblScope unit="page" to="802">802</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">7C5E9F2151B3619719CDA062907E775525236B54</idno><idno type="DOI">10.1093/jxb/erm367</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Fructans are water-soluble fructose oligomers and polymers that are based on sucrose, and have been implicated in protecting plants against water stress. Rice (Oryza sativa L.) is highly sensitive to chilling temperatures, and is not able to synthesize fructans. Two wheat fructan-synthesizing enzymes, sucrose:sucrose 1-fructosyltransferase, encoded by wft2, or sucrose:fructan 6-fructosyltransferase, encoded by wft1, were introduced into rice plants, and rice transformants that accumulate fructans were successfully obtained. The mature leaf blades of transgenic rice lines with wft2 or wft1 accumulated 16.2 mg g1 FW of oligo- and polysaccharides mainly composed of inulin oligomers of more than DP7, and 3.7 mg g1 FW of oligo- and polysaccharides, mainly composed of phlein oligomers of more than DP15, respectively. The transgenic rice seedlings with wft2 accumulated significantly higher concentrations of oligo- and polysaccharides than non-transgenic rice seedlings, and exhibited enhanced chilling tolerance. The oligo- and polysaccharide concentrations of seedlings expressing wft1 were obviously lower than those of lines expressing wft2, and no correlation between oligo- and polysaccharide concentrations and chilling tolerance was detected in wft1-expressing rice lines. The results suggest that transgenic rice lines expressing wheat-derived fructosyltransferase genes accumulated large amounts of fructans in mature leaf blades and exhibited enhanced chilling tolerance at the seedling stage. This is the first report owing that fructan accumulation enhanced tolerance to non-freezing low temperatures.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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