Proteome profiling of early seed development in Cunninghamia lanceolata (Lamb.) Hook
Identifieur interne : 001466 ( Istex/Curation ); précédent : 001465; suivant : 001467Proteome profiling of early seed development in Cunninghamia lanceolata (Lamb.) Hook
Auteurs : Jisen Shi [République populaire de Chine] ; Yan Zhen [République populaire de Chine] ; Ren-Hua Zheng [République populaire de Chine]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2010-05.
Abstract
Knowledge of the proteome of the early gymnosperm embryo could provide important information for optimizing plant cloning procedures and for establishing platforms for research into plant development/regulation and in vitro transgenic studies. Compared with angiosperms, it is more difficult to induce somatic embryogenesis in gymnosperms; success in this endeavour could be increased, however, if proteomic information was available on the complex, dynamic, and multistage processes of gymnosperm embryogenesis in vivo. A proteomic analysis of Chinese fir seeds in six developmental stages was carried out during early embryogenesis. Proteins were extracted from seeds dissected from immature cones and separated by two-dimensional difference gel electrophoresis. Analysis with DeCyder 6.5 software revealed 136 spots that differed in kinetics of appearance. Analysis by liquid chromatography coupled to tandem mass spectrometry and MALDI-TOF mass spectrometry identified proteins represented by 71 of the spots. Functional annotation of these seed proteins revealed their involvement in programmed cell death and chromatin modification, indicating that the proteins may play a central role in determining the number of zygotic embryos generated and controlling embryo patterning and shape remodelling. The analysis also revealed other proteins involved in carbon metabolism, methionine metabolism, energy production, protein storage, synthesis and stabilization, disease/defence, the cytoskeleton, and embryo development. The comprehensive protein expression profiles generated by our study provide new insights into the complex developmental processes in the seeds of the Chinese fir.
Url:
DOI: 10.1093/jxb/erq066
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001513
Links to Exploration step
ISTEX:FC4E1CB7E07AE6214DDDF8F3D1F0BEDE3A8FA0C0Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Proteome profiling of early seed development in Cunninghamia lanceolata (Lamb.) Hook</title><author><name sortKey="Shi, Jisen" sort="Shi, Jisen" uniqKey="Shi J" first="Jisen" last="Shi">Jisen Shi</name><affiliation wicri:level="1"><mods:affiliation>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: jshi@njfu.edu.cn</mods:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation></author><author><name sortKey="Zhen, Yan" sort="Zhen, Yan" uniqKey="Zhen Y" first="Yan" last="Zhen">Yan Zhen</name><affiliation wicri:level="1"><mods:affiliation>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037</wicri:regionArea></affiliation></author><author><name sortKey="Zheng, Ren Hua" sort="Zheng, Ren Hua" uniqKey="Zheng R" first="Ren-Hua" last="Zheng">Ren-Hua Zheng</name><affiliation wicri:level="1"><mods:affiliation>Fujian Provincial Academic of Forestry Science, Fuzhou 350003, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Fujian Provincial Academic of Forestry Science, Fuzhou 350003</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FC4E1CB7E07AE6214DDDF8F3D1F0BEDE3A8FA0C0</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1093/jxb/erq066</idno><idno type="url">https://api.istex.fr/document/FC4E1CB7E07AE6214DDDF8F3D1F0BEDE3A8FA0C0/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001513</idno><idno type="wicri:Area/Istex/Curation">001466</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Proteome profiling of early seed development in Cunninghamia lanceolata (Lamb.) Hook</title><author><name sortKey="Shi, Jisen" sort="Shi, Jisen" uniqKey="Shi J" first="Jisen" last="Shi">Jisen Shi</name><affiliation wicri:level="1"><mods:affiliation>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: jshi@njfu.edu.cn</mods:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation></author><author><name sortKey="Zhen, Yan" sort="Zhen, Yan" uniqKey="Zhen Y" first="Yan" last="Zhen">Yan Zhen</name><affiliation wicri:level="1"><mods:affiliation>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Forest Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037</wicri:regionArea></affiliation></author><author><name sortKey="Zheng, Ren Hua" sort="Zheng, Ren Hua" uniqKey="Zheng R" first="Ren-Hua" last="Zheng">Ren-Hua Zheng</name><affiliation wicri:level="1"><mods:affiliation>Fujian Provincial Academic of Forestry Science, Fuzhou 350003, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Fujian Provincial Academic of Forestry Science, Fuzhou 350003</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2010-05">2010-05</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="2367">2367</biblScope><biblScope unit="page" to="2381">2381</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">FC4E1CB7E07AE6214DDDF8F3D1F0BEDE3A8FA0C0</idno><idno type="DOI">10.1093/jxb/erq066</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Knowledge of the proteome of the early gymnosperm embryo could provide important information for optimizing plant cloning procedures and for establishing platforms for research into plant development/regulation and in vitro transgenic studies. Compared with angiosperms, it is more difficult to induce somatic embryogenesis in gymnosperms; success in this endeavour could be increased, however, if proteomic information was available on the complex, dynamic, and multistage processes of gymnosperm embryogenesis in vivo. A proteomic analysis of Chinese fir seeds in six developmental stages was carried out during early embryogenesis. Proteins were extracted from seeds dissected from immature cones and separated by two-dimensional difference gel electrophoresis. Analysis with DeCyder 6.5 software revealed 136 spots that differed in kinetics of appearance. Analysis by liquid chromatography coupled to tandem mass spectrometry and MALDI-TOF mass spectrometry identified proteins represented by 71 of the spots. Functional annotation of these seed proteins revealed their involvement in programmed cell death and chromatin modification, indicating that the proteins may play a central role in determining the number of zygotic embryos generated and controlling embryo patterning and shape remodelling. The analysis also revealed other proteins involved in carbon metabolism, methionine metabolism, energy production, protein storage, synthesis and stabilization, disease/defence, the cytoskeleton, and embryo development. The comprehensive protein expression profiles generated by our study provide new insights into the complex developmental processes in the seeds of the Chinese fir.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001466 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001466 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:FC4E1CB7E07AE6214DDDF8F3D1F0BEDE3A8FA0C0
|texte= Proteome profiling of early seed development in Cunninghamia lanceolata (Lamb.) Hook
}}
| This area was generated with Dilib version V0.6.25. |  |