Phylogenetic and molecular analysis of hydrogen-producing green algae
Identifieur interne : 001511 ( Istex/Corpus ); précédent : 001510; suivant : 001512Phylogenetic and molecular analysis of hydrogen-producing green algae
Auteurs : Matthew Timmins ; Skye R. Thomas-Hall ; Aaron Darling ; Eugene Zhang ; Ben Hankamer ; Ute C. Marx ; Peer M. SchenkSource :
- Journal of Experimental Botany [ 0022-0957 ] ; 2009-04-02.
Abstract
A select set of microalgae are reported to be able to catalyse photobiological H2 production from water. Based on the model organism Chlamydomonas reinhardtii, a method was developed for the screening of naturally occurring H2-producing microalgae. By purging algal cultures with N2 in the dark and subsequent illumination, it is possible to rapidly induce photobiological H2 evolution. Using NMR spectroscopy for metabolic profiling in C. reinhardtii, acetate, formate, and ethanol were found to be key compounds contributing to metabolic variance during the assay. This procedure can be used to test algal species existing as axenic or mixed cultures for their ability to produce H2. Using this system, five algal isolates capable of H2 production were identified in various aquatic systems. A phylogenetic tree was constructed using ribosomal sequence data of green unicellular algae to determine if there were taxonomic patterns of H2 production. H2-producing algal species were seen to be dispersed amongst most clades, indicating an H2-producing capacity preceded evolution of the phylum Chlorophyta.
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DOI: 10.1093/jxb/erp052
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Based on the model organism Chlamydomonas reinhardtii, a method was developed for the screening of naturally occurring H2-producing microalgae. By purging algal cultures with N2 in the dark and subsequent illumination, it is possible to rapidly induce photobiological H2 evolution. Using NMR spectroscopy for metabolic profiling in C. reinhardtii, acetate, formate, and ethanol were found to be key compounds contributing to metabolic variance during the assay. This procedure can be used to test algal species existing as axenic or mixed cultures for their ability to produce H2. Using this system, five algal isolates capable of H2 production were identified in various aquatic systems. A phylogenetic tree was constructed using ribosomal sequence data of green unicellular algae to determine if there were taxonomic patterns of H2 production. H2-producing algal species were seen to be dispersed amongst most clades, indicating an H2-producing capacity preceded evolution of the phylum Chlorophyta.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Matthew Timmins</name><affiliations><json:string>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</json:string></affiliations></json:item><json:item><name>Skye R. 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type="first">Aaron</forename><surname>Darling</surname></persName><affiliation>Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation></author><author xml:id="author-4"><persName><forename type="first">Eugene</forename><surname>Zhang</surname></persName><affiliation>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation></author><author xml:id="author-5"><persName><forename type="first">Ben</forename><surname>Hankamer</surname></persName><affiliation>Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation></author><author xml:id="author-6"><persName><forename type="first">Ute C.</forename><surname>Marx</surname></persName><affiliation>Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><affiliation>SRC for Functional and Applied Genomics, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation></author><author xml:id="author-7" corresp="yes"><persName><forename type="first">Peer M.</forename><surname>Schenk</surname></persName><email>p.schenk@uq.edu.au</email><affiliation>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation></author></analytic><monogr><title level="j">Journal of Experimental Botany</title><idno type="pISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-04-02"></date><biblScope unit="volume">60</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1691">1691</biblScope><biblScope unit="page" to="1702">1702</biblScope></imprint></monogr><idno type="istex">84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2</idno><idno 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H2-producing algal species were seen to be dispersed amongst most clades, indicating an H2-producing capacity preceded evolution of the phylum Chlorophyta.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Research Papers</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>Biohydrogen</term></item><item><term>Chlamydomonas reinhardtii</term></item><item><term>Chlorophyta</term></item><item><term>photobiological H2 production</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2009-04-02">Created</change><change when="2009-04-02">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">jexbot</journal-id><journal-id journal-id-type="publisher-id">exbotj</journal-id><journal-title>Journal of Experimental Botany</journal-title><issn pub-type="epub">1460-2431</issn><issn pub-type="ppub">0022-0957</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/jxb/erp052</article-id><article-categories><subj-group><subject>Research Papers</subject></subj-group></article-categories><title-group><article-title>Phylogenetic and molecular analysis of hydrogen-producing green algae</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Timmins</surname><given-names>Matthew</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Thomas-Hall</surname><given-names>Skye R.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Darling</surname><given-names>Aaron</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Eugene</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hankamer</surname><given-names>Ben</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Marx</surname><given-names>Ute C.</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Schenk</surname><given-names>Peer M.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1"><label>1</label>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</aff><aff id="aff2"><label>2</label>Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia</aff><aff id="aff3"><label>3</label>SRC for Functional and Applied Genomics, The University of Queensland, St Lucia, Queensland 4072, Australia</aff><author-notes><corresp id="cor1"><label>*</label>To whom correspondence should be addressed: E-mail: <email>p.schenk@uq.edu.au</email></corresp></author-notes><pub-date pub-type="epub-ppub"><month>4</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>2</day><month>4</month><year>2009</year></pub-date><volume>60</volume><issue>6</issue><fpage>1691</fpage><lpage>1702</lpage><history><date date-type="received"><day>10</day><month>10</month><year>2008</year></date><date date-type="rev-recd"><day>28</day><month>1</month><year>2009</year></date><date date-type="accepted"><day>29</day><month>1</month><year>2009</year></date></history><permissions><copyright-statement>© 2009 The Author(s).</copyright-statement><copyright-year>2009</copyright-year><license license-type="open-access"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</p><p>This paper is available online free of all access charges (see <ext-link ext-link-type="uri" xlink:href="http://jxb.oxfordjournals.org/open_access.html">http://jxb.oxfordjournals.org/open_access.html</ext-link> for further details)</p></license></permissions><abstract><p>A select set of microalgae are reported to be able to catalyse photobiological H<sub>2</sub> production from water. Based on the model organism <italic>Chlamydomonas reinhardtii</italic>, a method was developed for the screening of naturally occurring H<sub>2</sub>-producing microalgae. By purging algal cultures with N<sub>2</sub> in the dark and subsequent illumination, it is possible to rapidly induce photobiological H<sub>2</sub> evolution. Using NMR spectroscopy for metabolic profiling in <italic>C</italic>. <italic>reinhardtii</italic>, acetate, formate, and ethanol were found to be key compounds contributing to metabolic variance during the assay. This procedure can be used to test algal species existing as axenic or mixed cultures for their ability to produce H<sub>2</sub>. Using this system, five algal isolates capable of H<sub>2</sub> production were identified in various aquatic systems. A phylogenetic tree was constructed using ribosomal sequence data of green unicellular algae to determine if there were taxonomic patterns of H<sub>2</sub> production. H<sub>2</sub>-producing algal species were seen to be dispersed amongst most clades, indicating an H<sub>2</sub>-producing capacity preceded evolution of the phylum Chlorophyta.</p></abstract><kwd-group><kwd>Biohydrogen</kwd><kwd><italic>Chlamydomonas reinhardtii</italic></kwd><kwd>Chlorophyta</kwd><kwd>photobiological H<sub>2</sub> production</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Phylogenetic and molecular analysis of hydrogen-producing green algae</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Phylogenetic and molecular analysis of hydrogen-producing green algae</title></titleInfo><name type="personal"><namePart type="given">Matthew</namePart><namePart type="family">Timmins</namePart><affiliation>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Skye R.</namePart><namePart type="family">Thomas-Hall</namePart><affiliation>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Aaron</namePart><namePart type="family">Darling</namePart><affiliation>Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eugene</namePart><namePart type="family">Zhang</namePart><affiliation>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ben</namePart><namePart type="family">Hankamer</namePart><affiliation>Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ute C.</namePart><namePart type="family">Marx</namePart><affiliation>Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><affiliation>SRC for Functional and Applied Genomics, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Peer M.</namePart><namePart type="family">Schenk</namePart><affiliation>School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia</affiliation><affiliation>E-mail: p.schenk@uq.edu.au</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><topic>Research Papers</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2009-04-02</dateIssued><dateCreated encoding="w3cdtf">2009-04-02</dateCreated><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>A select set of microalgae are reported to be able to catalyse photobiological H2 production from water. Based on the model organism Chlamydomonas reinhardtii, a method was developed for the screening of naturally occurring H2-producing microalgae. By purging algal cultures with N2 in the dark and subsequent illumination, it is possible to rapidly induce photobiological H2 evolution. Using NMR spectroscopy for metabolic profiling in C. reinhardtii, acetate, formate, and ethanol were found to be key compounds contributing to metabolic variance during the assay. This procedure can be used to test algal species existing as axenic or mixed cultures for their ability to produce H2. Using this system, five algal isolates capable of H2 production were identified in various aquatic systems. A phylogenetic tree was constructed using ribosomal sequence data of green unicellular algae to determine if there were taxonomic patterns of H2 production. H2-producing algal species were seen to be dispersed amongst most clades, indicating an H2-producing capacity preceded evolution of the phylum Chlorophyta.</abstract><subject><genre>keywords</genre><topic>Biohydrogen</topic><topic>Chlamydomonas reinhardtii</topic><topic>Chlorophyta</topic><topic>photobiological H2 production</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Experimental Botany</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-0957</identifier><identifier type="eISSN">1460-2431</identifier><identifier type="PublisherID">exbotj</identifier><identifier type="PublisherID-hwp">jexbot</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>60</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>1691</start><end>1702</end></extent></part></relatedItem><identifier type="istex">84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2</identifier><identifier type="DOI">10.1093/jxb/erp052</identifier><accessCondition type="use and reproduction" contentType="open-access">This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/tiff</mimetype><extension>tiff</extension><uri>https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/covers/tiff</uri></json:item><json:item><original>true</original><mimetype>text/html</mimetype><extension>html</extension><uri>https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/covers/html</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/annexes/gif</uri></json:item><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/annexes/pdf</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/84A4AE566756BB6CBC7CC7D6B9F34ADC308F8DE2/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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