Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine
Identifieur interne : 001510 ( Istex/Corpus ); précédent : 001509; suivant : 001511Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine
Auteurs : Galle Cassin ; Stphane Mari ; Catherine Curie ; Jean-Franois Briat ; Pierre CzernicSource :
- Journal of Experimental Botany [ 0022-0957 ] ; 2009-02-02.
Abstract
Nicotianamine (NA) is a non-protein amino acid derivative synthesized from S-adenosyl L-methionine able to bind several metal ions such as iron, copper, manganese, zinc, or nickel. In plants, NA appears to be involved in iron availability and is essential for the plant to complete its biological cycle. In graminaceous plants, NA is also the precursor in the biosynthesis of phytosiderophores. Arabidopsis lines accumulating 4- and 100-fold more NA than wild-type plants were used in order to evaluate the impact of such an NA overaccumulation on iron homeostasis. The expression of iron-regulated genes including the IRT1/FRO2 iron uptake system is highly induced at the transcript level under both iron-sufficient and iron-deficient conditions. Nevertheless, NA overaccumulation does not interfere with the iron uptake mechanisms since the iron levels are similar in the NA-overaccumulating line and wild-type plants in both roots and leaves under both sufficient and deficient conditions. This observation also suggests that the translocation of iron from the root to the shoot is not affected in the NA-overaccumulating line. However, NA overaccumulation triggers an enhanced sensitivity to iron starvation, associated with a decrease in iron availability. This study draws attention to a particular phenotype where NA in excess paradoxically leads to iron deficiency, probably because of an increase of the NA apoplastic pool sequestering iron. This finding strengthens the notion that extracellular NA in the apoplast could be a major checkpoint to control plant iron homeostasis.
Url:
DOI: 10.1093/jxb/erp007
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine</title><author><name sortKey="Cassin, Galle" sort="Cassin, Galle" uniqKey="Cassin G" first="Galle" last="Cassin">Galle Cassin</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Mari, Stphane" sort="Mari, Stphane" uniqKey="Mari S" first="Stphane" last="Mari">Stphane Mari</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Curie, Catherine" sort="Curie, Catherine" uniqKey="Curie C" first="Catherine" last="Curie">Catherine Curie</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Briat, Jean Franois" sort="Briat, Jean Franois" uniqKey="Briat J" first="Jean-Franois" last="Briat">Jean-Franois Briat</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Czernic, Pierre" sort="Czernic, Pierre" uniqKey="Czernic P" first="Pierre" last="Czernic">Pierre Czernic</name><affiliation><mods:affiliation>E-mail: czernic@univ-montp2.fr</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4F90002DC87B248DB06913E35C6A2778BA7C438A</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1093/jxb/erp007</idno><idno type="url">https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001510</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine</title><author><name sortKey="Cassin, Galle" sort="Cassin, Galle" uniqKey="Cassin G" first="Galle" last="Cassin">Galle Cassin</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Mari, Stphane" sort="Mari, Stphane" uniqKey="Mari S" first="Stphane" last="Mari">Stphane Mari</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Curie, Catherine" sort="Curie, Catherine" uniqKey="Curie C" first="Catherine" last="Curie">Catherine Curie</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Briat, Jean Franois" sort="Briat, Jean Franois" uniqKey="Briat J" first="Jean-Franois" last="Briat">Jean-Franois Briat</name><affiliation><mods:affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</mods:affiliation></affiliation></author><author><name sortKey="Czernic, Pierre" sort="Czernic, Pierre" uniqKey="Czernic P" first="Pierre" last="Czernic">Pierre Czernic</name><affiliation><mods:affiliation>E-mail: czernic@univ-montp2.fr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-02-02">2009-02-02</date><biblScope unit="volume">60</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="1249">1249</biblScope><biblScope unit="page" to="1259">1259</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">4F90002DC87B248DB06913E35C6A2778BA7C438A</idno><idno type="DOI">10.1093/jxb/erp007</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Nicotianamine (NA) is a non-protein amino acid derivative synthesized from S-adenosyl L-methionine able to bind several metal ions such as iron, copper, manganese, zinc, or nickel. In plants, NA appears to be involved in iron availability and is essential for the plant to complete its biological cycle. In graminaceous plants, NA is also the precursor in the biosynthesis of phytosiderophores. Arabidopsis lines accumulating 4- and 100-fold more NA than wild-type plants were used in order to evaluate the impact of such an NA overaccumulation on iron homeostasis. The expression of iron-regulated genes including the IRT1/FRO2 iron uptake system is highly induced at the transcript level under both iron-sufficient and iron-deficient conditions. Nevertheless, NA overaccumulation does not interfere with the iron uptake mechanisms since the iron levels are similar in the NA-overaccumulating line and wild-type plants in both roots and leaves under both sufficient and deficient conditions. This observation also suggests that the translocation of iron from the root to the shoot is not affected in the NA-overaccumulating line. However, NA overaccumulation triggers an enhanced sensitivity to iron starvation, associated with a decrease in iron availability. This study draws attention to a particular phenotype where NA in excess paradoxically leads to iron deficiency, probably because of an increase of the NA apoplastic pool sequestering iron. This finding strengthens the notion that extracellular NA in the apoplast could be a major checkpoint to control plant iron homeostasis.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Galle Cassin</name><affiliations><json:string>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</json:string></affiliations></json:item><json:item><name>Stphane Mari</name><affiliations><json:string>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</json:string></affiliations></json:item><json:item><name>Catherine Curie</name><affiliations><json:string>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</json:string></affiliations></json:item><json:item><name>Jean-Franois Briat</name><affiliations><json:string>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</json:string></affiliations></json:item><json:item><name>Pierre Czernic</name><affiliations><json:string>E-mail: czernic@univ-montp2.fr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Research Papers</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Apoplast</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>iron homeostasis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>nicotianamine</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>Nicotianamine (NA) is a non-protein amino acid derivative synthesized from S-adenosyl L-methionine able to bind several metal ions such as iron, copper, manganese, zinc, or nickel. In plants, NA appears to be involved in iron availability and is essential for the plant to complete its biological cycle. In graminaceous plants, NA is also the precursor in the biosynthesis of phytosiderophores. Arabidopsis lines accumulating 4- and 100-fold more NA than wild-type plants were used in order to evaluate the impact of such an NA overaccumulation on iron homeostasis. The expression of iron-regulated genes including the IRT1/FRO2 iron uptake system is highly induced at the transcript level under both iron-sufficient and iron-deficient conditions. Nevertheless, NA overaccumulation does not interfere with the iron uptake mechanisms since the iron levels are similar in the NA-overaccumulating line and wild-type plants in both roots and leaves under both sufficient and deficient conditions. This observation also suggests that the translocation of iron from the root to the shoot is not affected in the NA-overaccumulating line. However, NA overaccumulation triggers an enhanced sensitivity to iron starvation, associated with a decrease in iron availability. This study draws attention to a particular phenotype where NA in excess paradoxically leads to iron deficiency, probably because of an increase of the NA apoplastic pool sequestering iron. This finding strengthens the notion that extracellular NA in the apoplast could be a major checkpoint to control plant iron homeostasis.</abstract><qualityIndicators><score>7.82</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 791 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>1587</abstractCharCount><pdfWordCount>6454</pdfWordCount><pdfCharCount>38926</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>235</abstractWordCount></qualityIndicators><title>Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine</title><genre><json:string>research-article</json:string></genre><host><volume>60</volume><publisherId><json:string>exbotj</json:string></publisherId><pages><last>1259</last><first>1249</first></pages><issn><json:string>0022-0957</json:string></issn><issue>4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1460-2431</json:string></eissn><title>Journal of Experimental Botany</title></host><categories><wos><json:string>PLANT SCIENCES</json:string></wos></categories><publicationDate>2009</publicationDate><copyrightDate>2009</copyrightDate><doi><json:string>10.1093/jxb/erp007</json:string></doi><id>4F90002DC87B248DB06913E35C6A2778BA7C438A</id><score>0.22619614</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability status="free"><p>Open Access</p></availability><date>2009-02-02</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine</title><author xml:id="author-1"><persName><forename type="first">Galle</forename><surname>Cassin</surname></persName><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">Stphane</forename><surname>Mari</surname></persName><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">Catherine</forename><surname>Curie</surname></persName><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Jean-Franois</forename><surname>Briat</surname></persName><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation></author><author xml:id="author-5" corresp="yes"><persName><forename type="first">Pierre</forename><surname>Czernic</surname></persName><email>czernic@univ-montp2.fr</email></author></analytic><monogr><title level="j">Journal of Experimental Botany</title><idno type="pISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-02-02"></date><biblScope unit="volume">60</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="1249">1249</biblScope><biblScope unit="page" to="1259">1259</biblScope></imprint></monogr><idno type="istex">4F90002DC87B248DB06913E35C6A2778BA7C438A</idno><idno type="DOI">10.1093/jxb/erp007</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009-02-02</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Nicotianamine (NA) is a non-protein amino acid derivative synthesized from S-adenosyl L-methionine able to bind several metal ions such as iron, copper, manganese, zinc, or nickel. In plants, NA appears to be involved in iron availability and is essential for the plant to complete its biological cycle. In graminaceous plants, NA is also the precursor in the biosynthesis of phytosiderophores. Arabidopsis lines accumulating 4- and 100-fold more NA than wild-type plants were used in order to evaluate the impact of such an NA overaccumulation on iron homeostasis. The expression of iron-regulated genes including the IRT1/FRO2 iron uptake system is highly induced at the transcript level under both iron-sufficient and iron-deficient conditions. Nevertheless, NA overaccumulation does not interfere with the iron uptake mechanisms since the iron levels are similar in the NA-overaccumulating line and wild-type plants in both roots and leaves under both sufficient and deficient conditions. This observation also suggests that the translocation of iron from the root to the shoot is not affected in the NA-overaccumulating line. However, NA overaccumulation triggers an enhanced sensitivity to iron starvation, associated with a decrease in iron availability. This study draws attention to a particular phenotype where NA in excess paradoxically leads to iron deficiency, probably because of an increase of the NA apoplastic pool sequestering iron. This finding strengthens the notion that extracellular NA in the apoplast could be a major checkpoint to control plant iron homeostasis.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Research Papers</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>Apoplast</term></item><item><term>iron homeostasis</term></item><item><term>nicotianamine</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2009-02-02">Created</change><change when="2009-02-02">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">jexbot</journal-id><journal-id journal-id-type="publisher-id">exbotj</journal-id><journal-title>Journal of Experimental Botany</journal-title><issn pub-type="epub">1460-2431</issn><issn pub-type="ppub">0022-0957</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/jxb/erp007</article-id><article-categories><subj-group><subject>Research Papers</subject></subj-group></article-categories><title-group><article-title>Increased sensitivity to iron deficiency in <italic>Arabidopsis thaliana</italic> overaccumulating nicotianamine</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Cassin</surname><given-names>Gaëlle</given-names></name></contrib><contrib contrib-type="author"><name><surname>Mari</surname><given-names>Stéphane</given-names></name></contrib><contrib contrib-type="author"><name><surname>Curie</surname><given-names>Catherine</given-names></name></contrib><contrib contrib-type="author"><name><surname>Briat</surname><given-names>Jean-François</given-names></name></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Czernic</surname><given-names>Pierre</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff>Biochimie et Physiologie Moléculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Université Montpellier 2, École Nationale Supérieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</aff><author-notes><corresp id="cor1"><label>*</label>To whom correspondence should be addressed. E-mail: <email>czernic@univ-montp2.fr</email></corresp></author-notes><pub-date pub-type="epub-ppub"><month>3</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>2</day><month>2</month><year>2009</year></pub-date><volume>60</volume><issue>4</issue><fpage>1249</fpage><lpage>1259</lpage><history><date date-type="received"><day>14</day><month>11</month><year>2008</year></date><date date-type="rev-recd"><day>19</day><month>12</month><year>2008</year></date><date date-type="accepted"><day>22</day><month>12</month><year>2008</year></date></history><permissions><copyright-statement>© 2009 The Author(s).</copyright-statement><copyright-year>2009</copyright-year><license license-type="open-access"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</p><p>This paper is available online free of all access charges (see <ext-link ext-link-type="uri" xlink:href="http://jxb.oxfordjournals.org/open_access.html">http://jxb.oxfordjournals.org/open_access.html</ext-link> for further details)</p></license></permissions><abstract><p>Nicotianamine (NA) is a non-protein amino acid derivative synthesized from <italic>S</italic>-adenosyl <sc>L</sc>-methionine able to bind several metal ions such as iron, copper, manganese, zinc, or nickel. In plants, NA appears to be involved in iron availability and is essential for the plant to complete its biological cycle. In graminaceous plants, NA is also the precursor in the biosynthesis of phytosiderophores. <italic>Arabidopsis</italic> lines accumulating 4- and 100-fold more NA than wild-type plants were used in order to evaluate the impact of such an NA overaccumulation on iron homeostasis. The expression of iron-regulated genes including the IRT1/FRO2 iron uptake system is highly induced at the transcript level under both iron-sufficient and iron-deficient conditions. Nevertheless, NA overaccumulation does not interfere with the iron uptake mechanisms since the iron levels are similar in the NA-overaccumulating line and wild-type plants in both roots and leaves under both sufficient and deficient conditions. This observation also suggests that the translocation of iron from the root to the shoot is not affected in the NA-overaccumulating line. However, NA overaccumulation triggers an enhanced sensitivity to iron starvation, associated with a decrease in iron availability. This study draws attention to a particular phenotype where NA in excess paradoxically leads to iron deficiency, probably because of an increase of the NA apoplastic pool sequestering iron. This finding strengthens the notion that extracellular NA in the apoplast could be a major checkpoint to control plant iron homeostasis.</p></abstract><kwd-group><kwd>Apoplast</kwd><kwd>iron homeostasis</kwd><kwd>nicotianamine</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine</title></titleInfo><name type="personal"><namePart type="given">Galle</namePart><namePart type="family">Cassin</namePart><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stphane</namePart><namePart type="family">Mari</namePart><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Catherine</namePart><namePart type="family">Curie</namePart><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean-Franois</namePart><namePart type="family">Briat</namePart><affiliation>Biochimie et Physiologie Molculaire des Plantes. Centre National de la Recherche Scientifique (UMR 5004), Institut National de la Recherche Agronomique, Universit Montpellier 2, cole Nationale Suprieure d'Agronomie, 2 Place Viala, F-34060 Montpellier cedex 2, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Pierre</namePart><namePart type="family">Czernic</namePart><affiliation>E-mail: czernic@univ-montp2.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><topic>Research Papers</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2009-02-02</dateIssued><dateCreated encoding="w3cdtf">2009-02-02</dateCreated><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Nicotianamine (NA) is a non-protein amino acid derivative synthesized from S-adenosyl L-methionine able to bind several metal ions such as iron, copper, manganese, zinc, or nickel. In plants, NA appears to be involved in iron availability and is essential for the plant to complete its biological cycle. In graminaceous plants, NA is also the precursor in the biosynthesis of phytosiderophores. Arabidopsis lines accumulating 4- and 100-fold more NA than wild-type plants were used in order to evaluate the impact of such an NA overaccumulation on iron homeostasis. The expression of iron-regulated genes including the IRT1/FRO2 iron uptake system is highly induced at the transcript level under both iron-sufficient and iron-deficient conditions. Nevertheless, NA overaccumulation does not interfere with the iron uptake mechanisms since the iron levels are similar in the NA-overaccumulating line and wild-type plants in both roots and leaves under both sufficient and deficient conditions. This observation also suggests that the translocation of iron from the root to the shoot is not affected in the NA-overaccumulating line. However, NA overaccumulation triggers an enhanced sensitivity to iron starvation, associated with a decrease in iron availability. This study draws attention to a particular phenotype where NA in excess paradoxically leads to iron deficiency, probably because of an increase of the NA apoplastic pool sequestering iron. This finding strengthens the notion that extracellular NA in the apoplast could be a major checkpoint to control plant iron homeostasis.</abstract><subject><genre>keywords</genre><topic>Apoplast</topic><topic>iron homeostasis</topic><topic>nicotianamine</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Experimental Botany</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-0957</identifier><identifier type="eISSN">1460-2431</identifier><identifier type="PublisherID">exbotj</identifier><identifier type="PublisherID-hwp">jexbot</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>60</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>1249</start><end>1259</end></extent></part></relatedItem><identifier type="istex">4F90002DC87B248DB06913E35C6A2778BA7C438A</identifier><identifier type="DOI">10.1093/jxb/erp007</identifier><accessCondition type="use and reproduction" contentType="open-access">This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/tiff</mimetype><extension>tiff</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/covers/tiff</uri></json:item><json:item><original>true</original><mimetype>text/html</mimetype><extension>html</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/covers/html</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/annexes/gif</uri></json:item><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/annexes/pdf</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/4F90002DC87B248DB06913E35C6A2778BA7C438A/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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