Assessment of Tissue-Engineered Islet Graft Viability by Fluorine Magnetic Resonance Spectroscopy
Identifieur interne : 000128 ( PascalFrancis/Checkpoint ); précédent : 000127; suivant : 000129Assessment of Tissue-Engineered Islet Graft Viability by Fluorine Magnetic Resonance Spectroscopy
Auteurs : T. M. Suszynski [États-Unis] ; E. S. Avgoustiniatos [États-Unis] ; S. A. Stein [États-Unis] ; E. J. Falde [États-Unis] ; B. E. Hammer [États-Unis] ; K. K. Papas [États-Unis]Source :
- Transplantation proceedings [ 0041-1345 ] ; 2011.
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Abstract
Introduction. Despite significant progress in the last decade, islet transplantation remains an experimental therapy for a limited number of patients with type 1 diabetes. Tissue-engineered approaches may provide promising alternatives to the current clinical protocol and would benefit greatly from concurrent development of graft quality assessment techniques. This study was designed to evaluate whether viability of tissue-engineered islet grafts can be assessed using fluorine magnetic resonance spectroscopy (19F-MRS), by the noninvasive measurement of oxygen partial pressure (pO2) and the subsequent calculation of islet oxygen consumption rate (OCR). Methods. Scaffolds composed of porcine plasma were seeded with human islets and perfluorodecalin. Each graft was covered with the same volume of culture media in a Petri dish. Four scaffolds were seeded with various numbers (0-8000) of islet equivalents (IE) aliquoted from the same preparation. After randomizing run order, grafts were examined by 19F-MRS at 37°C using a 5T spectrometer and a single-loop surface coil placed underneath. A standard inversion recovery sequence was used to obtain characteristic 19F spin-lattice relaxation times (T1), which were converted to steady-state average pO2 estimates using a previously determined linear calibration (R2 = 1.000). Each condition was assessed using replicate 19F-MRS measurements (n = 6-8). Results. Grafts exhibited IE dose-dependent increases in T1 and decreases in pO2 estimates. From the difference between scaffold pO2 estimates and ambient pO2, the islet preparation OCR was calculated to be 95 ± 12 (mean ± standard error of the mean) nmol/(min . mg DNA) using theoretical modeling. This value compared well with OCR values measured using established methods for human islet preparations. Conclusions. 19F-MRS can be used for noninvasive pre- and possibly posttransplant assessment of tissue-engineered islet graft viability by estimating the amount of viable, oxygen-consuming tissue in a scaffold.
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<term>NMR spectrometry</term>
<term>Tissue culture</term>
<term>Tissue engineering</term>
<term>Transplantation</term>
<term>Treatment</term>
<term>Viability</term>
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<term>Culture tissu</term>
<term>Génie tissulaire</term>
<term>Spectrométrie RMN</term>
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<front><div type="abstract" xml:lang="en">Introduction. Despite significant progress in the last decade, islet transplantation remains an experimental therapy for a limited number of patients with type 1 diabetes. Tissue-engineered approaches may provide promising alternatives to the current clinical protocol and would benefit greatly from concurrent development of graft quality assessment techniques. This study was designed to evaluate whether viability of tissue-engineered islet grafts can be assessed using fluorine magnetic resonance spectroscopy (<sup>19</sup>
F-MRS), by the noninvasive measurement of oxygen partial pressure (pO<sub>2</sub>
) and the subsequent calculation of islet oxygen consumption rate (OCR). Methods. Scaffolds composed of porcine plasma were seeded with human islets and perfluorodecalin. Each graft was covered with the same volume of culture media in a Petri dish. Four scaffolds were seeded with various numbers (0-8000) of islet equivalents (IE) aliquoted from the same preparation. After randomizing run order, grafts were examined by <sup>19</sup>
F-MRS at 37°C using a 5T spectrometer and a single-loop surface coil placed underneath. A standard inversion recovery sequence was used to obtain characteristic <sup>19</sup>
F spin-lattice relaxation times (T1), which were converted to steady-state average pO<sub>2</sub>
estimates using a previously determined linear calibration (R<sup>2</sup>
= 1.000). Each condition was assessed using replicate <sup>19</sup>
F-MRS measurements (n = 6-8). Results. Grafts exhibited IE dose-dependent increases in T1 and decreases in pO<sub>2</sub>
estimates. From the difference between scaffold pO<sub>2</sub>
estimates and ambient pO<sub>2</sub>
, the islet preparation OCR was calculated to be 95 ± 12 (mean ± standard error of the mean) nmol/(min . mg DNA) using theoretical modeling. This value compared well with OCR values measured using established methods for human islet preparations. Conclusions. <sup>19</sup>
F-MRS can be used for noninvasive pre- and possibly posttransplant assessment of tissue-engineered islet graft viability by estimating the amount of viable, oxygen-consuming tissue in a scaffold.</div>
</front>
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<fA08 i1="01" i2="1" l="ENG"><s1>Assessment of Tissue-Engineered Islet Graft Viability by Fluorine Magnetic Resonance Spectroscopy</s1>
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<fC01 i1="01" l="ENG"><s0>Introduction. Despite significant progress in the last decade, islet transplantation remains an experimental therapy for a limited number of patients with type 1 diabetes. Tissue-engineered approaches may provide promising alternatives to the current clinical protocol and would benefit greatly from concurrent development of graft quality assessment techniques. This study was designed to evaluate whether viability of tissue-engineered islet grafts can be assessed using fluorine magnetic resonance spectroscopy (<sup>19</sup>
F-MRS), by the noninvasive measurement of oxygen partial pressure (pO<sub>2</sub>
) and the subsequent calculation of islet oxygen consumption rate (OCR). Methods. Scaffolds composed of porcine plasma were seeded with human islets and perfluorodecalin. Each graft was covered with the same volume of culture media in a Petri dish. Four scaffolds were seeded with various numbers (0-8000) of islet equivalents (IE) aliquoted from the same preparation. After randomizing run order, grafts were examined by <sup>19</sup>
F-MRS at 37°C using a 5T spectrometer and a single-loop surface coil placed underneath. A standard inversion recovery sequence was used to obtain characteristic <sup>19</sup>
F spin-lattice relaxation times (T1), which were converted to steady-state average pO<sub>2</sub>
estimates using a previously determined linear calibration (R<sup>2</sup>
= 1.000). Each condition was assessed using replicate <sup>19</sup>
F-MRS measurements (n = 6-8). Results. Grafts exhibited IE dose-dependent increases in T1 and decreases in pO<sub>2</sub>
estimates. From the difference between scaffold pO<sub>2</sub>
estimates and ambient pO<sub>2</sub>
, the islet preparation OCR was calculated to be 95 ± 12 (mean ± standard error of the mean) nmol/(min . mg DNA) using theoretical modeling. This value compared well with OCR values measured using established methods for human islet preparations. Conclusions. <sup>19</sup>
F-MRS can be used for noninvasive pre- and possibly posttransplant assessment of tissue-engineered islet graft viability by estimating the amount of viable, oxygen-consuming tissue in a scaffold.</s0>
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<fC07 i1="02" i2="X" l="ENG"><s0>Surgery</s0>
<s5>38</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Cirugía</s0>
<s5>38</s5>
</fC07>
<fN21><s1>002</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>World Congress of the International Pancreas and Islet Transplant Association (IPITA)</s1>
<s3>CZE</s3>
<s4>2011-06-01</s4>
</fA30>
</pR>
</standard>
</inist>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Minnesota</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Minnesota"><name sortKey="Suszynski, T M" sort="Suszynski, T M" uniqKey="Suszynski T" first="T. M." last="Suszynski">T. M. Suszynski</name>
</region>
<name sortKey="Avgoustiniatos, E S" sort="Avgoustiniatos, E S" uniqKey="Avgoustiniatos E" first="E. S." last="Avgoustiniatos">E. S. Avgoustiniatos</name>
<name sortKey="Falde, E J" sort="Falde, E J" uniqKey="Falde E" first="E. J." last="Falde">E. J. Falde</name>
<name sortKey="Hammer, B E" sort="Hammer, B E" uniqKey="Hammer B" first="B. E." last="Hammer">B. E. Hammer</name>
<name sortKey="Papas, K K" sort="Papas, K K" uniqKey="Papas K" first="K. K" last="Papas">K. K. Papas</name>
<name sortKey="Stein, S A" sort="Stein, S A" uniqKey="Stein S" first="S. A." last="Stein">S. A. Stein</name>
</country>
</tree>
</affiliations>
</record>
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