Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice
Identifieur interne : 000F38 ( Main/Exploration ); précédent : 000F37; suivant : 000F39Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice
Auteurs : K. K. Papas [États-Unis] ; C. K. Colton [États-Unis] ; R. A. Nelson [États-Unis] ; P. R. Rozak [États-Unis] ; E. S. Avgoustiniatos [États-Unis] ; W. E. Iii Scott [États-Unis] ; G. M. Wildey [États-Unis] ; A. Pisania [États-Unis] ; G. C. Weir [États-Unis] ; B. J. Hering [États-Unis]Source :
- American journal of transplantation [ 1600-6135 ] ; 2007.
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Abstract
There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.
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Iii Scott</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Wildey, G M" sort="Wildey, G M" uniqKey="Wildey G" first="G. M." last="Wildey">G. M. Wildey</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Pisania, A" sort="Pisania, A" uniqKey="Pisania A" first="A." last="Pisania">A. Pisania</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Chemical Engineering, Massachusetts Institute of Technology</s1><s2>Cambridge, MA</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Weir, G C" sort="Weir, G C" uniqKey="Weir G" first="G. C." last="Weir">G. C. Weir</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Joslin Diabetes Center, Harvard Medical School</s1><s2>Boston, MA</s2><s3>USA</s3><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Hering, B J" sort="Hering, B J" uniqKey="Hering B" first="B. J." last="Hering">B. J. Hering</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0165894</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0165894 INIST</idno><idno type="RBID">Pascal:07-0165894</idno><idno type="wicri:Area/PascalFrancis/Corpus">000352</idno><idno type="wicri:Area/PascalFrancis/Curation">000434</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000280</idno><idno type="wicri:doubleKey">1600-6135:2007:Papas K:human:islet:oxygen</idno><idno type="wicri:Area/Main/Merge">000F51</idno><idno type="wicri:Area/Main/Curation">000F38</idno><idno type="wicri:Area/Main/Exploration">000F38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice</title><author><name sortKey="Papas, K K" sort="Papas, K K" uniqKey="Papas K" first="K. K." last="Papas">K. K. Papas</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Colton, C K" sort="Colton, C K" uniqKey="Colton C" first="C. K." last="Colton">C. K. Colton</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Chemical Engineering, Massachusetts Institute of Technology</s1><s2>Cambridge, MA</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Nelson, R A" sort="Nelson, R A" uniqKey="Nelson R" first="R. A." last="Nelson">R. A. Nelson</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Administrative and Bioinformatics Coordinating Center (ABCC) for the Islet Cell Resource (ICR) Center Consortium, City of Hope National Medical Center and Beckman Research Institute</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Rozak, P R" sort="Rozak, P R" uniqKey="Rozak P" first="P. R." last="Rozak">P. R. Rozak</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Avgoustiniatos, E S" sort="Avgoustiniatos, E S" uniqKey="Avgoustiniatos E" first="E. S." last="Avgoustiniatos">E. S. Avgoustiniatos</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Scott, W E Iii" sort="Scott, W E Iii" uniqKey="Scott W" first="W. E. Iii" last="Scott">W. E. Iii Scott</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Wildey, G M" sort="Wildey, G M" uniqKey="Wildey G" first="G. M." last="Wildey">G. M. Wildey</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Pisania, A" sort="Pisania, A" uniqKey="Pisania A" first="A." last="Pisania">A. Pisania</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Chemical Engineering, Massachusetts Institute of Technology</s1><s2>Cambridge, MA</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Weir, G C" sort="Weir, G C" uniqKey="Weir G" first="G. C." last="Weir">G. C. Weir</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Joslin Diabetes Center, Harvard Medical School</s1><s2>Boston, MA</s2><s3>USA</s3><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Hering, B J" sort="Hering, B J" uniqKey="Hering B" first="B. J." last="Hering">B. J. Hering</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1><s2>Minneapolis, MN</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Minnesota</region></placeName></affiliation></author></analytic><series><title level="j" type="main">American journal of transplantation</title><idno type="ISSN">1600-6135</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">American journal of transplantation</title><idno type="ISSN">1600-6135</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term><term>DNA</term><term>Diabetes mellitus</term><term>Epidemiology</term><term>Human</term><term>Langerhans islet</term><term>Measurement</term><term>Mouse</term><term>Oxygen consumption</term><term>Prediction</term><term>Predictive factor</term><term>Quality control</term><term>Rate</term><term>Reversibility</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Mesure</term><term>Homme</term><term>Ilot Langerhans</term><term>Diabète</term><term>Consommation oxygène</term><term>Taux</term><term>Epidémiologie</term><term>DNA</term><term>Facteur prédictif</term><term>Prédiction</term><term>Réversibilité</term><term>Animal</term><term>Souris</term><term>Contrôle qualité</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Diabète</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Massachusetts</li><li>Minnesota</li></region></list><tree><country name="États-Unis"><region name="Minnesota"><name sortKey="Papas, K K" sort="Papas, K K" uniqKey="Papas K" first="K. K." last="Papas">K. K. Papas</name></region><name sortKey="Avgoustiniatos, E S" sort="Avgoustiniatos, E S" uniqKey="Avgoustiniatos E" first="E. S." last="Avgoustiniatos">E. S. Avgoustiniatos</name><name sortKey="Colton, C K" sort="Colton, C K" uniqKey="Colton C" first="C. K." last="Colton">C. K. Colton</name><name sortKey="Hering, B J" sort="Hering, B J" uniqKey="Hering B" first="B. J." last="Hering">B. J. Hering</name><name sortKey="Nelson, R A" sort="Nelson, R A" uniqKey="Nelson R" first="R. A." last="Nelson">R. A. Nelson</name><name sortKey="Pisania, A" sort="Pisania, A" uniqKey="Pisania A" first="A." last="Pisania">A. Pisania</name><name sortKey="Rozak, P R" sort="Rozak, P R" uniqKey="Rozak P" first="P. R." last="Rozak">P. R. Rozak</name><name sortKey="Scott, W E Iii" sort="Scott, W E Iii" uniqKey="Scott W" first="W. E. Iii" last="Scott">W. E. Iii Scott</name><name sortKey="Weir, G C" sort="Weir, G C" uniqKey="Weir G" first="G. C." last="Weir">G. C. Weir</name><name sortKey="Wildey, G M" sort="Wildey, G M" uniqKey="Wildey G" first="G. M." last="Wildey">G. M. Wildey</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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