Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice
Identifieur interne : 000F38 ( Main/Exploration ); précédent : 000F37; suivant : 000F39Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice
Auteurs : K. K. Papas [États-Unis] ; C. K. Colton [États-Unis] ; R. A. Nelson [États-Unis] ; P. R. Rozak [États-Unis] ; E. S. Avgoustiniatos [États-Unis] ; W. E. Iii Scott [États-Unis] ; G. M. Wildey [États-Unis] ; A. Pisania [États-Unis] ; G. C. Weir [États-Unis] ; B. J. Hering [États-Unis]Source :
- American journal of transplantation [ 1600-6135 ] ; 2007.
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- Pascal (Inist)
- Wicri :
English descriptors
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Abstract
There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.
Affiliations:
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term>
<term>DNA</term>
<term>Diabetes mellitus</term>
<term>Epidemiology</term>
<term>Human</term>
<term>Langerhans islet</term>
<term>Measurement</term>
<term>Mouse</term>
<term>Oxygen consumption</term>
<term>Prediction</term>
<term>Predictive factor</term>
<term>Quality control</term>
<term>Rate</term>
<term>Reversibility</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Mesure</term>
<term>Homme</term>
<term>Ilot Langerhans</term>
<term>Diabète</term>
<term>Consommation oxygène</term>
<term>Taux</term>
<term>Epidémiologie</term>
<term>DNA</term>
<term>Facteur prédictif</term>
<term>Prédiction</term>
<term>Réversibilité</term>
<term>Animal</term>
<term>Souris</term>
<term>Contrôle qualité</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
<term>Diabète</term>
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<front><div type="abstract" xml:lang="en">There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.</div>
</front>
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