Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice
Identifieur interne : 000352 ( PascalFrancis/Corpus ); précédent : 000351; suivant : 000353Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice
Auteurs : K. K. Papas ; C. K. Colton ; R. A. Nelson ; P. R. Rozak ; E. S. Avgoustiniatos ; W. E. Iii Scott ; G. M. Wildey ; A. Pisania ; G. C. Weir ; B. J. HeringSource :
- American journal of transplantation [ 1600-6135 ] ; 2007.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.
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Format Inist (serveur)
NO : | PASCAL 07-0165894 INIST |
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ET : | Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice |
AU : | PAPAS (K. K.); COLTON (C. K.); NELSON (R. A.); ROZAK (P. R.); AVGOUSTINIATOS (E. S.); SCOTT (W. E. III); WILDEY (G. M.); PISANIA (A.); WEIR (G. C.); HERING (B. J.) |
AF : | Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota/Minneapolis, MN/Etats-Unis (1 aut., 4 aut., 5 aut., 6 aut., 7 aut., 10 aut.); Department of Chemical Engineering, Massachusetts Institute of Technology/Cambridge, MA/Etats-Unis (2 aut., 8 aut.); Administrative and Bioinformatics Coordinating Center (ABCC) for the Islet Cell Resource (ICR) Center Consortium, City of Hope National Medical Center and Beckman Research Institute/Duarte, CA/Etats-Unis (3 aut.); Joslin Diabetes Center, Harvard Medical School/Boston, MA/Etats-Unis (9 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | American journal of transplantation; ISSN 1600-6135; Royaume-Uni; Da. 2007; Vol. 7; No. 3; Pp. 707-713; Bibl. 24 ref. |
LA : | Anglais |
EA : | There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation. |
CC : | 002B25; 002B30A01A; 002B21E01A |
FD : | Mesure; Homme; Ilot Langerhans; Diabète; Consommation oxygène; Taux; Epidémiologie; DNA; Facteur prédictif; Prédiction; Réversibilité; Animal; Souris; Contrôle qualité |
FG : | Rodentia; Mammalia; Vertebrata; Pancréas endocrine; Endocrinopathie |
ED : | Measurement; Human; Langerhans islet; Diabetes mellitus; Oxygen consumption; Rate; Epidemiology; DNA; Predictive factor; Prediction; Reversibility; Animal; Mouse; Quality control |
EG : | Rodentia; Mammalia; Vertebrata; Endocrine pancreas; Endocrinopathy |
SD : | Medida; Hombre; Isla Langerhans; Diabetes; Consumo oxígeno; Tasa; Epidemiología; DNA; Factor predictivo; Predicción; Reversibilidad; Animal; Ratón; Control calidad |
LO : | INIST-27587.354000159369750280 |
ID : | 07-0165894 |
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Pascal:07-0165894Le document en format XML
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<term>Langerhans islet</term>
<term>Measurement</term>
<term>Mouse</term>
<term>Oxygen consumption</term>
<term>Prediction</term>
<term>Predictive factor</term>
<term>Quality control</term>
<term>Rate</term>
<term>Reversibility</term>
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<term>Ilot Langerhans</term>
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<front><div type="abstract" xml:lang="en">There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.</div>
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<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Diabète</s0>
<s2>NM</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Diabetes mellitus</s0>
<s2>NM</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Diabetes</s0>
<s2>NM</s2>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Consommation oxygène</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Oxygen consumption</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Consumo oxígeno</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Taux</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Rate</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Tasa</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Epidémiologie</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Epidemiology</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Epidemiología</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>DNA</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>DNA</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>DNA</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Facteur prédictif</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Predictive factor</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Factor predictivo</s0>
<s5>11</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Prédiction</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Prediction</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Predicción</s0>
<s5>12</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Réversibilité</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Reversibility</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Reversibilidad</s0>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Animal</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Animal</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Animal</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Souris</s0>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Mouse</s0>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Ratón</s0>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Contrôle qualité</s0>
<s5>20</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Quality control</s0>
<s5>20</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Control calidad</s0>
<s5>20</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Pancréas endocrine</s0>
<s5>37</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Endocrine pancreas</s0>
<s5>37</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Páncreas endocrino</s0>
<s5>37</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Endocrinopathie</s0>
<s5>38</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Endocrinopathy</s0>
<s5>38</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Endocrinopatía</s0>
<s5>38</s5>
</fC07>
<fN21><s1>106</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 07-0165894 INIST</NO>
<ET>Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice</ET>
<AU>PAPAS (K. K.); COLTON (C. K.); NELSON (R. A.); ROZAK (P. R.); AVGOUSTINIATOS (E. S.); SCOTT (W. E. III); WILDEY (G. M.); PISANIA (A.); WEIR (G. C.); HERING (B. J.)</AU>
<AF>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota/Minneapolis, MN/Etats-Unis (1 aut., 4 aut., 5 aut., 6 aut., 7 aut., 10 aut.); Department of Chemical Engineering, Massachusetts Institute of Technology/Cambridge, MA/Etats-Unis (2 aut., 8 aut.); Administrative and Bioinformatics Coordinating Center (ABCC) for the Islet Cell Resource (ICR) Center Consortium, City of Hope National Medical Center and Beckman Research Institute/Duarte, CA/Etats-Unis (3 aut.); Joslin Diabetes Center, Harvard Medical School/Boston, MA/Etats-Unis (9 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>American journal of transplantation; ISSN 1600-6135; Royaume-Uni; Da. 2007; Vol. 7; No. 3; Pp. 707-713; Bibl. 24 ref.</SO>
<LA>Anglais</LA>
<EA>There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.</EA>
<CC>002B25; 002B30A01A; 002B21E01A</CC>
<FD>Mesure; Homme; Ilot Langerhans; Diabète; Consommation oxygène; Taux; Epidémiologie; DNA; Facteur prédictif; Prédiction; Réversibilité; Animal; Souris; Contrôle qualité</FD>
<FG>Rodentia; Mammalia; Vertebrata; Pancréas endocrine; Endocrinopathie</FG>
<ED>Measurement; Human; Langerhans islet; Diabetes mellitus; Oxygen consumption; Rate; Epidemiology; DNA; Predictive factor; Prediction; Reversibility; Animal; Mouse; Quality control</ED>
<EG>Rodentia; Mammalia; Vertebrata; Endocrine pancreas; Endocrinopathy</EG>
<SD>Medida; Hombre; Isla Langerhans; Diabetes; Consumo oxígeno; Tasa; Epidemiología; DNA; Factor predictivo; Predicción; Reversibilidad; Animal; Ratón; Control calidad</SD>
<LO>INIST-27587.354000159369750280</LO>
<ID>07-0165894</ID>
</server>
</inist>
</record>
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