OcrV1, PascalFrancis, Corpus, bibRecord, 000352

Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice

Identifieur interne : 000352 ( PascalFrancis/Corpus ); précédent : 000351; suivant : 000353

Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice

Auteurs : K. K. Papas ; C. K. Colton ; R. A. Nelson ; P. R. Rozak ; E. S. Avgoustiniatos ; W. E. Iii Scott ; G. M. Wildey ; A. Pisania ; G. C. Weir ; B. J. Hering

Source :

American journal of transplantation [ 1600-6135 ] ; 2007.

RBID : Pascal:07-0165894

Descripteurs français

Pascal (Inist)
- Mesure, Homme, Ilot Langerhans, Diabète, Consommation oxygène, Taux, Epidémiologie, DNA, Facteur prédictif, Prédiction, Réversibilité, Animal, Souris, Contrôle qualité.

English descriptors

KwdEn :
- Animal, DNA, Diabetes mellitus, Epidemiology, Human, Langerhans islet, Measurement, Mouse, Oxygen consumption, Prediction, Predictive factor, Quality control, Rate, Reversibility.

Abstract

There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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Format Inist (serveur)

NO :	PASCAL 07-0165894 INIST
ET :	Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice
AU :	PAPAS (K. K.); COLTON (C. K.); NELSON (R. A.); ROZAK (P. R.); AVGOUSTINIATOS (E. S.); SCOTT (W. E. III); WILDEY (G. M.); PISANIA (A.); WEIR (G. C.); HERING (B. J.)
AF :	Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota/Minneapolis, MN/Etats-Unis (1 aut., 4 aut., 5 aut., 6 aut., 7 aut., 10 aut.); Department of Chemical Engineering, Massachusetts Institute of Technology/Cambridge, MA/Etats-Unis (2 aut., 8 aut.); Administrative and Bioinformatics Coordinating Center (ABCC) for the Islet Cell Resource (ICR) Center Consortium, City of Hope National Medical Center and Beckman Research Institute/Duarte, CA/Etats-Unis (3 aut.); Joslin Diabetes Center, Harvard Medical School/Boston, MA/Etats-Unis (9 aut.)
DT :	Publication en série; Niveau analytique
SO :	American journal of transplantation; ISSN 1600-6135; Royaume-Uni; Da. 2007; Vol. 7; No. 3; Pp. 707-713; Bibl. 24 ref.
LA :	Anglais
EA :	There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.
CC :	002B25; 002B30A01A; 002B21E01A
FD :	Mesure; Homme; Ilot Langerhans; Diabète; Consommation oxygène; Taux; Epidémiologie; DNA; Facteur prédictif; Prédiction; Réversibilité; Animal; Souris; Contrôle qualité
FG :	Rodentia; Mammalia; Vertebrata; Pancréas endocrine; Endocrinopathie
ED :	Measurement; Human; Langerhans islet; Diabetes mellitus; Oxygen consumption; Rate; Epidemiology; DNA; Predictive factor; Prediction; Reversibility; Animal; Mouse; Quality control
EG :	Rodentia; Mammalia; Vertebrata; Endocrine pancreas; Endocrinopathy
SD :	Medida; Hombre; Isla Langerhans; Diabetes; Consumo oxígeno; Tasa; Epidemiología; DNA; Factor predictivo; Predicción; Reversibilidad; Animal; Ratón; Control calidad
LO :	INIST-27587.354000159369750280
ID :	07-0165894

Links to Exploration step

Pascal:07-0165894

Le document en format XML

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<author><name sortKey="Pisania, A" sort="Pisania, A" uniqKey="Pisania A" first="A." last="Pisania">A. Pisania</name>
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<series><title level="j" type="main">American journal of transplantation</title>
<idno type="ISSN">1600-6135</idno>
<imprint><date when="2007">2007</date>
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<term>DNA</term>
<term>Diabetes mellitus</term>
<term>Epidemiology</term>
<term>Human</term>
<term>Langerhans islet</term>
<term>Measurement</term>
<term>Mouse</term>
<term>Oxygen consumption</term>
<term>Prediction</term>
<term>Predictive factor</term>
<term>Quality control</term>
<term>Rate</term>
<term>Reversibility</term>
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<term>Ilot Langerhans</term>
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<term>Taux</term>
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<front><div type="abstract" xml:lang="en">There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.</div>
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<fA11 i1="10" i2="1"><s1>HERING (B. J.)</s1>
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<fA14 i1="01"><s1>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota</s1>
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<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
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<sZ>8 aut.</sZ>
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<s5>18</s5>
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<s5>18</s5>
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<s5>18</s5>
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<s5>19</s5>
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<ET>Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice</ET>
<AU>PAPAS (K. K.); COLTON (C. K.); NELSON (R. A.); ROZAK (P. R.); AVGOUSTINIATOS (E. S.); SCOTT (W. E. III); WILDEY (G. M.); PISANIA (A.); WEIR (G. C.); HERING (B. J.)</AU>
<AF>Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota/Minneapolis, MN/Etats-Unis (1 aut., 4 aut., 5 aut., 6 aut., 7 aut., 10 aut.); Department of Chemical Engineering, Massachusetts Institute of Technology/Cambridge, MA/Etats-Unis (2 aut., 8 aut.); Administrative and Bioinformatics Coordinating Center (ABCC) for the Islet Cell Resource (ICR) Center Consortium, City of Hope National Medical Center and Beckman Research Institute/Duarte, CA/Etats-Unis (3 aut.); Joslin Diabetes Center, Harvard Medical School/Boston, MA/Etats-Unis (9 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>American journal of transplantation; ISSN 1600-6135; Royaume-Uni; Da. 2007; Vol. 7; No. 3; Pp. 707-713; Bibl. 24 ref.</SO>
<LA>Anglais</LA>
<EA>There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.</EA>
<CC>002B25; 002B30A01A; 002B21E01A</CC>
<FD>Mesure; Homme; Ilot Langerhans; Diabète; Consommation oxygène; Taux; Epidémiologie; DNA; Facteur prédictif; Prédiction; Réversibilité; Animal; Souris; Contrôle qualité</FD>
<FG>Rodentia; Mammalia; Vertebrata; Pancréas endocrine; Endocrinopathie</FG>
<ED>Measurement; Human; Langerhans islet; Diabetes mellitus; Oxygen consumption; Rate; Epidemiology; DNA; Predictive factor; Prediction; Reversibility; Animal; Mouse; Quality control</ED>
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Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice

Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice

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